Click here to switch to the map view.

The map label for this gene is metK

Identifier: 170750882

GI number: 170750882

Start: 4773502

End: 4774680

Strand: Direct

Name: metK

Synonym: Mrad2831_4493

Alternate gene names: 170750882

Gene position: 4773502-4774680 (Clockwise)

Preceding gene: 170750881

Following gene: 170750883

Centisome position: 78.54

GC content: 70.14

Gene sequence:

>1179_bases
ATGCCGCGTTCCAACTACCTGTTCACCAGCGAGTCCGTGTCCGAGGGACACCCCGACAAGGTCTGCGACAGGATCTCGGA
CACCGTGGTCGACGCCTACCTCGCGGCGATGCCGGAGGCGCGGCTCGGCGTCGAGACGCTCGCCACGACCAACCGCATCG
TCATCGCCGGCGAGGTGCGCGGACCCGATTCGGTGACCTTCAAGGACCTCGAGGCCCTTACCCGCGAGGCCGTGAAGGAC
ATCGGCTACGAGCAGTCGGGCTTCCACTGGAAGAACAACGACGTCGCGATCCACCTGCACGCCCAGTCGGCCGACATCGC
CCAGGGCGTGGACGCGGCCGGCAACAAGGACGAGGGCGCGGGCGACCAGGGCATCATGTTCGGCTACGCCGCCGACGAGA
CGCCCGAGCTGATGCCGGCGCCGATCTTCTACGCCCACAAGATCCTCAAGGACCTCGCCGACGCCCGCAAGGCGAGGCAG
GGCGACGCCGCCAAGCTCGGCCCCGACGCCAAGAGCCAGGTTACGGTCCGCTACGAGAACGGCCGCCCGGTCGAGGTCAC
CCAGATCGTGCTGTCGACCCAGCACCTCGACGAGTCCCTCGACTCCGCGGACGTGCGCGCCATCGTCGAGCCCTACATCC
TGAAGGCGCTGCCGCAGGGCTGGGTCAACGAGGGCACGGTCTGGCACGTGAACCCGACCGGCAAGTTCGTGATCGGCGGC
CCGGACGGCGACGCCGGCCTCACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCGCGGCCCCGCACGGCGGCGGCGC
GTTCTCCGGCAAGGACCCGACGAAGGTCGACCGCTCGGCGGCCTACGCGGCGCGCTACCTCGCCAAGAACGTGGTGGCCG
CGGGCCTCGCCAGCCGCGCCACGATCCAGCTCGCCTACGCGATCGGCGTCTCGAAGCCGCTCTCGATCTACGTGGACCTG
CACGGCACCGGCACGGTCGACGAGGCCAAGCTCGAGGCCGTGCTGATGGACGCCATGGACCTGTCGCCCCGCGGCATCCG
TACGGCGCTCAGCCTGAACAAGCCGATCTACGCGCGCACCTCGGCCTACGGCCATTTCGGCCGCGCCCCCGAGGCCGACG
GCGGCTTCTCGTGGGAGCGCACCGACCTCGCCGGCAAGCTGAAGTCGGCGCTGGCCTGA

Upstream 100 bases:

>100_bases
TCCATCTTGACCGCCTCCCGGTCGGGTGCGAGAACCCCGGCCCCATGAAGGGTCAAACGCCGCGATCGCGACGGCGTACC
TCTGCAGCCGAGGCCGTCCG

Downstream 100 bases:

>100_bases
GGGCGGCCTCCGCGCGGCCCCGGGCCGACGCGCGGGCCGAGATCGGCGGCGCGCCGGGGCGACGTGAACGCGGGGCGGGC
GGTGCGCTGAGCGCCGCCCG

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPRSNYLFTSESVSEGHPDKVCDRISDTVVDAYLAAMPEARLGVETLATTNRIVIAGEVRGPDSVTFKDLEALTREAVKD
IGYEQSGFHWKNNDVAIHLHAQSADIAQGVDAAGNKDEGAGDQGIMFGYAADETPELMPAPIFYAHKILKDLADARKARQ
GDAAKLGPDAKSQVTVRYENGRPVEVTQIVLSTQHLDESLDSADVRAIVEPYILKALPQGWVNEGTVWHVNPTGKFVIGG
PDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLASRATIQLAYAIGVSKPLSIYVDL
HGTGTVDEAKLEAVLMDAMDLSPRGIRTALSLNKPIYARTSAYGHFGRAPEADGGFSWERTDLAGKLKSALA

Sequences:

>Translated_392_residues
MPRSNYLFTSESVSEGHPDKVCDRISDTVVDAYLAAMPEARLGVETLATTNRIVIAGEVRGPDSVTFKDLEALTREAVKD
IGYEQSGFHWKNNDVAIHLHAQSADIAQGVDAAGNKDEGAGDQGIMFGYAADETPELMPAPIFYAHKILKDLADARKARQ
GDAAKLGPDAKSQVTVRYENGRPVEVTQIVLSTQHLDESLDSADVRAIVEPYILKALPQGWVNEGTVWHVNPTGKFVIGG
PDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLASRATIQLAYAIGVSKPLSIYVDL
HGTGTVDEAKLEAVLMDAMDLSPRGIRTALSLNKPIYARTSAYGHFGRAPEADGGFSWERTDLAGKLKSALA
>Mature_391_residues
PRSNYLFTSESVSEGHPDKVCDRISDTVVDAYLAAMPEARLGVETLATTNRIVIAGEVRGPDSVTFKDLEALTREAVKDI
GYEQSGFHWKNNDVAIHLHAQSADIAQGVDAAGNKDEGAGDQGIMFGYAADETPELMPAPIFYAHKILKDLADARKARQG
DAAKLGPDAKSQVTVRYENGRPVEVTQIVLSTQHLDESLDSADVRAIVEPYILKALPQGWVNEGTVWHVNPTGKFVIGGP
DGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLASRATIQLAYAIGVSKPLSIYVDLH
GTGTVDEAKLEAVLMDAMDLSPRGIRTALSLNKPIYARTSAYGHFGRAPEADGGFSWERTDLAGKLKSALA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=383, Percent_Identity=51.9582245430809, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI4557737, Length=381, Percent_Identity=50.1312335958005, Blast_Score=336, Evalue=3e-92,
Organism=Escherichia coli, GI1789311, Length=389, Percent_Identity=51.9280205655527, Blast_Score=380, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=49.8714652956298, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=49.6143958868895, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25145633, Length=397, Percent_Identity=49.1183879093199, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17551082, Length=384, Percent_Identity=47.65625, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32565905, Length=387, Percent_Identity=45.9948320413437, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=48.3679525222552, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI25145635, Length=345, Percent_Identity=47.8260869565217, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=49.8349834983498, Blast_Score=254, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI32565909, Length=92, Percent_Identity=47.8260869565217, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320710, Length=384, Percent_Identity=48.1770833333333, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6323209, Length=384, Percent_Identity=48.1770833333333, Blast_Score=328, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24580527, Length=386, Percent_Identity=48.7046632124352, Blast_Score=318, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24580525, Length=386, Percent_Identity=48.7046632124352, Blast_Score=318, Evalue=3e-87,
Organism=Drosophila melanogaster, GI45552159, Length=384, Percent_Identity=48.9583333333333, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24580529, Length=384, Percent_Identity=48.9583333333333, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24580537, Length=384, Percent_Identity=48.9583333333333, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24580533, Length=384, Percent_Identity=48.9583333333333, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24580535, Length=384, Percent_Identity=48.9583333333333, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24580531, Length=384, Percent_Identity=48.9583333333333, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24580541, Length=383, Percent_Identity=46.7362924281984, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24580539, Length=123, Percent_Identity=45.5284552845528, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_METRJ (B1M4Z8)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001757142.1
- ProteinModelPortal:   B1M4Z8
- SMR:   B1M4Z8
- GeneID:   6140559
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_4493
- HOGENOM:   HBG443662
- OMA:   QDGFHWK
- ProtClustDB:   CLSK2331649
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41702; Mature: 41571

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSNYLFTSESVSEGHPDKVCDRISDTVVDAYLAAMPEARLGVETLATTNRIVIAGEVR
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCEEEEEEECC
GPDSVTFKDLEALTREAVKDIGYEQSGFHWKNNDVAIHLHAQSADIAQGVDAAGNKDEGA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCHHHHCCCCCCCCCCCC
GDQGIMFGYAADETPELMPAPIFYAHKILKDLADARKARQGDAAKLGPDAKSQVTVRYEN
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
GRPVEVTQIVLSTQHLDESLDSADVRAIVEPYILKALPQGWVNEGTVWHVNPTGKFVIGG
CCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEC
PDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLASRA
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TIQLAYAIGVSKPLSIYVDLHGTGTVDEAKLEAVLMDAMDLSPRGIRTALSLNKPIYART
EEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEC
SAYGHFGRAPEADGGFSWERTDLAGKLKSALA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PRSNYLFTSESVSEGHPDKVCDRISDTVVDAYLAAMPEARLGVETLATTNRIVIAGEVR
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCEEEEEEECC
GPDSVTFKDLEALTREAVKDIGYEQSGFHWKNNDVAIHLHAQSADIAQGVDAAGNKDEGA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCHHHHCCCCCCCCCCCC
GDQGIMFGYAADETPELMPAPIFYAHKILKDLADARKARQGDAAKLGPDAKSQVTVRYEN
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
GRPVEVTQIVLSTQHLDESLDSADVRAIVEPYILKALPQGWVNEGTVWHVNPTGKFVIGG
CCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEC
PDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGLASRA
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TIQLAYAIGVSKPLSIYVDLHGTGTVDEAKLEAVLMDAMDLSPRGIRTALSLNKPIYART
EEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEC
SAYGHFGRAPEADGGFSWERTDLAGKLKSALA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA