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The map label for this gene is gsiB [H]

Identifier: 170750863

GI number: 170750863

Start: 4754029

End: 4755645

Strand: Direct

Name: gsiB [H]

Synonym: Mrad2831_4474

Alternate gene names: 170750863

Gene position: 4754029-4755645 (Clockwise)

Preceding gene: 170750862

Following gene: 170750864

Centisome position: 78.22

GC content: 66.42

Gene sequence:

>1617_bases
TTGTCCCGTTCAGCCCTGAAGATTCTCGCCCGCGGCGTGTCCGCGGTCGCGGTCGCGACGTTCGCGTTCGCCGCGCTGCC
CGCGCAGGCCGCCAACGTCTTCCGCTTCGCCTTCCAGGGTGACCTGAAATCCCTCGATCCCTACTCCCTCAAGGAGAGCT
TCACGGAAGGGATGCAGGAGGCGGCCTACGAGCCCCTCGTCACCCTCGACAAGAACCTGAAATTCGCGCCCGCCCTCGCG
GAATCCTGGGAGACGCCCGAGCCGACGCGCTGGCGCTTCCACCTGCGCAAGAACGTCAAGTTCCACGACGGCTCGCCGTT
CACCGCCGACGACGTGATCTTCTCCGCCCAGCGCGTCCGCGCGCCGGGCTCGAACTTCACGACCAACGTGCCCGCCGACG
CCGAGTTCGTGAAGGTCGACGACTACACCGTCGACATGGTGCTGAAGAAGCCGAACCCCATCGCCATCGCCCAGTTCCCG
ACCTGGGTTATCATGTCGAAAGCGTGGTCCGAGAAGAACGGCGTCGTCCAGCCGACGCCGCCCAGCGCCACCAGCCCGAG
CTACGCGACGCTCCACGAGAACGGCACCGGCCCGTTCGTGATCGCCGAGCACCAGCCCGGCGTGAAGACCGTCTTCAAGA
AGTTCGACGGCTACTGGGGCAAGGTCGAGTCGAACCTCGACGAGGCGGTGCTGACCACCATCGCCAACCCGGCGACCCGC
GTCGCGGCGCTGCTCTCCGGCGAGGTCGACTGGATCGACCCCGTGCCGCTGCAGGACCAGCAGCGGGTCAACGCCAGCGG
CACCGCCACCGTCATGGCCGGCCCTGAACTCCGCACGATCTTCCTCGGCATGGACCAGGACCGGGACGAGCTCAAGGATT
CGAGCGTCAAGGGCAAGAACCCGTTCAAGGACATCAAGGTCCGCGAGGCCTTCTACCTCGCCATCGACGAGGACACGATC
GCCAAGCGCGTGATGCGCGGGCAGGCGGTGCCCTCCGCGCTGATGATCGCCCCGGCCCTCTACGACCGCGGCGCCGAGTT
CAAGCGCCCGGCCACCGACCTGAAGAAGGCCAAGGAGCTGATGGCCCAAGCGGGCTACCCGGACGGGTTCTCGCTCACCA
TGGACTGCCCCAACGACCGCTACGTCAACGACGAGGCGATCTGCCAGGCGGTGGTCTCGATGCTGGCGCGTATCAACGTC
AAGGTGAACCTGAACGCCCAGCCGAAGGCCAAGTACTTCGCCAAGGTTCTGGCGCCGAACTACGACACCTCGTTCTACCT
GCTCGGCTGGACCCCGTCCTCGCTCGACAGCCACAACATCCTCTACGAGATCGTCGGCTGCCGGAAGCCCGGCGACAAGT
CGGGCCGCGGCGGCTGGAACCTCGCGGGCTACTGCGATCCGAAGATCGACGAGATCGCCGACAAGGTCGAGGGCGAGACC
GACAAGACCAAGCGCGACGCCCTGATCAAGGAGGGCTTCGACGTCCTCAACGCCGATTGGGGCTACATCCCGCTGCACCA
GCAGGCGCTCGCCTGGGGCGTCTCCAAGAAGGTCCACCTGACCCAGCGCGCCGACAACCTGCTCCTGCTCTACTGGGTGT
CGAAGGACCCGCAGTAG

Upstream 100 bases:

>100_bases
GCCTTCGTCTTGTATACAGCTGGTCCGAAGCTTGCTGTCGCCGCGGTGGACACCGCCGGGGCTGCCGGCCGGTGGCGCAT
TCACGGTCTCGGAGATCCGC

Downstream 100 bases:

>100_bases
GAGCGCGGCGTGCTCGCCTTCCTGCTGCGCCGGGTGATCCAGGCGGCCGCGGTCCTCGCGGTCGTCGGGCTCATCGCCTT
CGCGATGTTCCGGTTCGCGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MSRSALKILARGVSAVAVATFAFAALPAQAANVFRFAFQGDLKSLDPYSLKESFTEGMQEAAYEPLVTLDKNLKFAPALA
ESWETPEPTRWRFHLRKNVKFHDGSPFTADDVIFSAQRVRAPGSNFTTNVPADAEFVKVDDYTVDMVLKKPNPIAIAQFP
TWVIMSKAWSEKNGVVQPTPPSATSPSYATLHENGTGPFVIAEHQPGVKTVFKKFDGYWGKVESNLDEAVLTTIANPATR
VAALLSGEVDWIDPVPLQDQQRVNASGTATVMAGPELRTIFLGMDQDRDELKDSSVKGKNPFKDIKVREAFYLAIDEDTI
AKRVMRGQAVPSALMIAPALYDRGAEFKRPATDLKKAKELMAQAGYPDGFSLTMDCPNDRYVNDEAICQAVVSMLARINV
KVNLNAQPKAKYFAKVLAPNYDTSFYLLGWTPSSLDSHNILYEIVGCRKPGDKSGRGGWNLAGYCDPKIDEIADKVEGET
DKTKRDALIKEGFDVLNADWGYIPLHQQALAWGVSKKVHLTQRADNLLLLYWVSKDPQ

Sequences:

>Translated_538_residues
MSRSALKILARGVSAVAVATFAFAALPAQAANVFRFAFQGDLKSLDPYSLKESFTEGMQEAAYEPLVTLDKNLKFAPALA
ESWETPEPTRWRFHLRKNVKFHDGSPFTADDVIFSAQRVRAPGSNFTTNVPADAEFVKVDDYTVDMVLKKPNPIAIAQFP
TWVIMSKAWSEKNGVVQPTPPSATSPSYATLHENGTGPFVIAEHQPGVKTVFKKFDGYWGKVESNLDEAVLTTIANPATR
VAALLSGEVDWIDPVPLQDQQRVNASGTATVMAGPELRTIFLGMDQDRDELKDSSVKGKNPFKDIKVREAFYLAIDEDTI
AKRVMRGQAVPSALMIAPALYDRGAEFKRPATDLKKAKELMAQAGYPDGFSLTMDCPNDRYVNDEAICQAVVSMLARINV
KVNLNAQPKAKYFAKVLAPNYDTSFYLLGWTPSSLDSHNILYEIVGCRKPGDKSGRGGWNLAGYCDPKIDEIADKVEGET
DKTKRDALIKEGFDVLNADWGYIPLHQQALAWGVSKKVHLTQRADNLLLLYWVSKDPQ
>Mature_537_residues
SRSALKILARGVSAVAVATFAFAALPAQAANVFRFAFQGDLKSLDPYSLKESFTEGMQEAAYEPLVTLDKNLKFAPALAE
SWETPEPTRWRFHLRKNVKFHDGSPFTADDVIFSAQRVRAPGSNFTTNVPADAEFVKVDDYTVDMVLKKPNPIAIAQFPT
WVIMSKAWSEKNGVVQPTPPSATSPSYATLHENGTGPFVIAEHQPGVKTVFKKFDGYWGKVESNLDEAVLTTIANPATRV
AALLSGEVDWIDPVPLQDQQRVNASGTATVMAGPELRTIFLGMDQDRDELKDSSVKGKNPFKDIKVREAFYLAIDEDTIA
KRVMRGQAVPSALMIAPALYDRGAEFKRPATDLKKAKELMAQAGYPDGFSLTMDCPNDRYVNDEAICQAVVSMLARINVK
VNLNAQPKAKYFAKVLAPNYDTSFYLLGWTPSSLDSHNILYEIVGCRKPGDKSGRGGWNLAGYCDPKIDEIADKVEGETD
KTKRDALIKEGFDVLNADWGYIPLHQQALAWGVSKKVHLTQRADNLLLLYWVSKDPQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=523, Percent_Identity=25.2390057361377, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1789966, Length=522, Percent_Identity=25.4789272030651, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787495, Length=496, Percent_Identity=23.7903225806452, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789397, Length=532, Percent_Identity=24.4360902255639, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789887, Length=530, Percent_Identity=22.4528301886792, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787762, Length=442, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787551, Length=534, Percent_Identity=22.8464419475655, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081878, Length=420, Percent_Identity=22.3809523809524, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59435; Mature: 59304

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSALKILARGVSAVAVATFAFAALPAQAANVFRFAFQGDLKSLDPYSLKESFTEGMQE
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHH
AAYEPLVTLDKNLKFAPALAESWETPEPTRWRFHLRKNVKFHDGSPFTADDVIFSAQRVR
HHCCCEEEECCCCEECCHHHHCCCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHC
APGSNFTTNVPADAEFVKVDDYTVDMVLKKPNPIAIAQFPTWVIMSKAWSEKNGVVQPTP
CCCCCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEECCCCEEEEEHHHCCCCCCCCCCC
PSATSPSYATLHENGTGPFVIAEHQPGVKTVFKKFDGYWGKVESNLDEAVLTTIANPATR
CCCCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHCCHHHH
VAALLSGEVDWIDPVPLQDQQRVNASGTATVMAGPELRTIFLGMDQDRDELKDSSVKGKN
HHHHHCCCCCCCCCCCCCCHHCCCCCCCEEEEECCCEEEEEEECCCCHHHHHCCCCCCCC
PFKDIKVREAFYLAIDEDTIAKRVMRGQAVPSALMIAPALYDRGAEFKRPATDLKKAKEL
CCCCCEEEEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
MAQAGYPDGFSLTMDCPNDRYVNDEAICQAVVSMLARINVKVNLNAQPKAKYFAKVLAPN
HHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCC
YDTSFYLLGWTPSSLDSHNILYEIVGCRKPGDKSGRGGWNLAGYCDPKIDEIADKVEGET
CCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
DKTKRDALIKEGFDVLNADWGYIPLHQQALAWGVSKKVHLTQRADNLLLLYWVSKDPQ
CHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCC
>Mature Secondary Structure 
SRSALKILARGVSAVAVATFAFAALPAQAANVFRFAFQGDLKSLDPYSLKESFTEGMQE
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHH
AAYEPLVTLDKNLKFAPALAESWETPEPTRWRFHLRKNVKFHDGSPFTADDVIFSAQRVR
HHCCCEEEECCCCEECCHHHHCCCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHC
APGSNFTTNVPADAEFVKVDDYTVDMVLKKPNPIAIAQFPTWVIMSKAWSEKNGVVQPTP
CCCCCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEECCCCEEEEEHHHCCCCCCCCCCC
PSATSPSYATLHENGTGPFVIAEHQPGVKTVFKKFDGYWGKVESNLDEAVLTTIANPATR
CCCCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHCCHHHH
VAALLSGEVDWIDPVPLQDQQRVNASGTATVMAGPELRTIFLGMDQDRDELKDSSVKGKN
HHHHHCCCCCCCCCCCCCCHHCCCCCCCEEEEECCCEEEEEEECCCCHHHHHCCCCCCCC
PFKDIKVREAFYLAIDEDTIAKRVMRGQAVPSALMIAPALYDRGAEFKRPATDLKKAKEL
CCCCCEEEEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
MAQAGYPDGFSLTMDCPNDRYVNDEAICQAVVSMLARINVKVNLNAQPKAKYFAKVLAPN
HHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCC
YDTSFYLLGWTPSSLDSHNILYEIVGCRKPGDKSGRGGWNLAGYCDPKIDEIADKVEGET
CCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
DKTKRDALIKEGFDVLNADWGYIPLHQQALAWGVSKKVHLTQRADNLLLLYWVSKDPQ
CHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA