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The map label for this gene is mepA [H]

Identifier: 170750861

GI number: 170750861

Start: 4749164

End: 4750507

Strand: Direct

Name: mepA [H]

Synonym: Mrad2831_4472

Alternate gene names: 170750861

Gene position: 4749164-4750507 (Clockwise)

Preceding gene: 170750860

Following gene: 170750862

Centisome position: 78.14

GC content: 72.54

Gene sequence:

>1344_bases
ATGCGCGGGCCCATGCTCCGCGCCAGCCTCAGCAGACGCATGCTTTCAGGAGCGTCGGTAATCGTGACCCGCACTTTGTC
CGCCAGAATCCCGTCGTCGTTCCGCGCCTCGGCCCGCGCGACCGCCCTCCTGCCCGCGGCCCTGCTCCTGCTGGGCGTCG
CCGCCTGCAACCCGAAGCAGGTCGCCGCGCCGACCCCGCCGGTGCCGGAGGTCGGGTTCGTCAAGGTGGAGCCCCAGGCG
ATCCCCTACCTGCGCGACCTGCCGGGCCGCGTCGCGCCGATGCGCATCGCCGAGGTCCGCTCCCGGGTCTCCGGGCTCGT
GGTCAAGCGGCTCTTCGAGCAGGGCAGCCAGGTCAAGGAGGGCGACATCCTCTACAAGATCGACCCGGCGCCCTACGAGG
TCGAGCTCGCCAGCGCCGAAGCGGCCCTCGCCCGCCAGGAGGCCGCCCTGGTGCTCGCCCGCCAGCAGGCGGACCGGCTC
GAGCAGCTGCTCTCCCGCGCCACCGCGAGCCAGGCGCAGTACGACGCCGCCTTCGCGGCCAAGAAGCAGGCGGAGGCCGA
GGTGGCCGGCGCCAAGGCGACCCGCGACCGGGCGCAGCTCAACCTCGGCTGGACCGACGTGCGCGCGCCGATCACCGGCC
GCATCGGCCGGGCGCTGCTCACCGAGGGTACCCTGATCGAGCCGGGCTCCACCGGCGCGCTCGCCACGATCCAGCAGCTC
GACCCGATCTACGTCGACATCACCCAGTCGGTTGGCGAACTGAACCGCCTGCGCCGGGACCTCGCCAGCGGCGAGCTGGC
GCGGCTCGAGGACAACACCGCCAACGTCCACCTGATCATGGACGACGGCTCGCTCTACCCGCTGGCCGGTCGGCTGCTGT
TCTCGGACGTCACCGCCGATCCGAGCACCGGCCAGGTGACCCTGCGGGTCCAGTTCCCGAACCCGCACGACGAGCTGTTC
CCCGGCATGTACGTGCGGGCGCGGATCAAGCAGGGCATCGATTCCGACGCCATCGCGGTGCCGCAGCAGGCGATCCAGCG
CACCGACGACGGCCGCGCCGAGGTCTGGATCGTGCGCGCCGACGAGACCGTGATGCGCCAGCCCGTCGAGGTCGGGCCGG
TGGTCGGGCAGAACTGGCTGATCCGTTCGGGCCTCAAGGCCGGCGAGCGCGTCATCATCGACGGCTTCCAGAAGATCACC
GTCGGCGCCAAGGTGAAGCCCCTCGACCAGACCCCGGTCCACGGCGAGACCGGACCGAAGCACGACACCGACGAGGCCCC
CGACGTGCCGGGCGACAAGGCGGAGGTCGCCCCGCGGGCCGCCGCCGTCCAGCAGCGCCGCTGA

Upstream 100 bases:

>100_bases
GGCCTGACGCGCGCCCCGGTCTCGCGGCCGGCATGAAGGCGCGCTCGTCCTGATCTTCGTCTGACCGGCCCCGTGACCGG
TCCCGGGATCTGACTCGACG

Downstream 100 bases:

>100_bases
GTCCGGGAGAGCCCGATCATGGCACGCTTCTTCATCGACCGCCCGGTCTTCGCCTGGGTGGTGGCGCTGTTCATCTGCCT
GGGCGGCGCCCTCGCGATCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MRGPMLRASLSRRMLSGASVIVTRTLSARIPSSFRASARATALLPAALLLLGVAACNPKQVAAPTPPVPEVGFVKVEPQA
IPYLRDLPGRVAPMRIAEVRSRVSGLVVKRLFEQGSQVKEGDILYKIDPAPYEVELASAEAALARQEAALVLARQQADRL
EQLLSRATASQAQYDAAFAAKKQAEAEVAGAKATRDRAQLNLGWTDVRAPITGRIGRALLTEGTLIEPGSTGALATIQQL
DPIYVDITQSVGELNRLRRDLASGELARLEDNTANVHLIMDDGSLYPLAGRLLFSDVTADPSTGQVTLRVQFPNPHDELF
PGMYVRARIKQGIDSDAIAVPQQAIQRTDDGRAEVWIVRADETVMRQPVEVGPVVGQNWLIRSGLKAGERVIIDGFQKIT
VGAKVKPLDQTPVHGETGPKHDTDEAPDVPGDKAEVAPRAAAVQQRR

Sequences:

>Translated_447_residues
MRGPMLRASLSRRMLSGASVIVTRTLSARIPSSFRASARATALLPAALLLLGVAACNPKQVAAPTPPVPEVGFVKVEPQA
IPYLRDLPGRVAPMRIAEVRSRVSGLVVKRLFEQGSQVKEGDILYKIDPAPYEVELASAEAALARQEAALVLARQQADRL
EQLLSRATASQAQYDAAFAAKKQAEAEVAGAKATRDRAQLNLGWTDVRAPITGRIGRALLTEGTLIEPGSTGALATIQQL
DPIYVDITQSVGELNRLRRDLASGELARLEDNTANVHLIMDDGSLYPLAGRLLFSDVTADPSTGQVTLRVQFPNPHDELF
PGMYVRARIKQGIDSDAIAVPQQAIQRTDDGRAEVWIVRADETVMRQPVEVGPVVGQNWLIRSGLKAGERVIIDGFQKIT
VGAKVKPLDQTPVHGETGPKHDTDEAPDVPGDKAEVAPRAAAVQQRR
>Mature_447_residues
MRGPMLRASLSRRMLSGASVIVTRTLSARIPSSFRASARATALLPAALLLLGVAACNPKQVAAPTPPVPEVGFVKVEPQA
IPYLRDLPGRVAPMRIAEVRSRVSGLVVKRLFEQGSQVKEGDILYKIDPAPYEVELASAEAALARQEAALVLARQQADRL
EQLLSRATASQAQYDAAFAAKKQAEAEVAGAKATRDRAQLNLGWTDVRAPITGRIGRALLTEGTLIEPGSTGALATIQQL
DPIYVDITQSVGELNRLRRDLASGELARLEDNTANVHLIMDDGSLYPLAGRLLFSDVTADPSTGQVTLRVQFPNPHDELF
PGMYVRARIKQGIDSDAIAVPQQAIQRTDDGRAEVWIVRADETVMRQPVEVGPVVGQNWLIRSGLKAGERVIIDGFQKIT
VGAKVKPLDQTPVHGETGPKHDTDEAPDVPGDKAEVAPRAAAVQQRR

Specific function: The periplasmic linker protein component of an organic solvent and antibiotic efflux pump; confers resistance to toluene, hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin and tetracycline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=374, Percent_Identity=46.524064171123, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1786668, Length=364, Percent_Identity=45.3296703296703, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1789929, Length=385, Percent_Identity=38.4415584415584, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI87082036, Length=349, Percent_Identity=30.945558739255, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI87081951, Length=134, Percent_Identity=31.3432835820896, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48149; Mature: 48149

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGPMLRASLSRRMLSGASVIVTRTLSARIPSSFRASARATALLPAALLLLGVAACNPKQ
CCCCCHHHHHHHHHHCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VAAPTPPVPEVGFVKVEPQAIPYLRDLPGRVAPMRIAEVRSRVSGLVVKRLFEQGSQVKE
CCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GDILYKIDPAPYEVELASAEAALARQEAALVLARQQADRLEQLLSRATASQAQYDAAFAA
CCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKQAEAEVAGAKATRDRAQLNLGWTDVRAPITGRIGRALLTEGTLIEPGSTGALATIQQL
HHHHHHHHHCCHHCCCHHEEECCHHHCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHC
DPIYVDITQSVGELNRLRRDLASGELARLEDNTANVHLIMDDGSLYPLAGRLLFSDVTAD
CCEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCHHHHEEEECCCCC
PSTGQVTLRVQFPNPHDELFPGMYVRARIKQGIDSDAIAVPQQAIQRTDDGRAEVWIVRA
CCCCEEEEEEECCCCHHHHCCCHHHHHHHHCCCCCCHHHCCHHHHHCCCCCCEEEEEEEC
DETVMRQPVEVGPVVGQNWLIRSGLKAGERVIIDGFQKITVGAKVKPLDQTPVHGETGPK
CHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEEEECCEEEEECCEECCCCCCCCCCCCCCC
HDTDEAPDVPGDKAEVAPRAAAVQQRR
CCCCCCCCCCCCCHHHCHHHHHHHCCC
>Mature Secondary Structure
MRGPMLRASLSRRMLSGASVIVTRTLSARIPSSFRASARATALLPAALLLLGVAACNPKQ
CCCCCHHHHHHHHHHCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VAAPTPPVPEVGFVKVEPQAIPYLRDLPGRVAPMRIAEVRSRVSGLVVKRLFEQGSQVKE
CCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GDILYKIDPAPYEVELASAEAALARQEAALVLARQQADRLEQLLSRATASQAQYDAAFAA
CCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKQAEAEVAGAKATRDRAQLNLGWTDVRAPITGRIGRALLTEGTLIEPGSTGALATIQQL
HHHHHHHHHCCHHCCCHHEEECCHHHCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHC
DPIYVDITQSVGELNRLRRDLASGELARLEDNTANVHLIMDDGSLYPLAGRLLFSDVTAD
CCEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCHHHHEEEECCCCC
PSTGQVTLRVQFPNPHDELFPGMYVRARIKQGIDSDAIAVPQQAIQRTDDGRAEVWIVRA
CCCCEEEEEEECCCCHHHHCCCHHHHHHHHCCCCCCHHHCCHHHHHCCCCCCEEEEEEEC
DETVMRQPVEVGPVVGQNWLIRSGLKAGERVIIDGFQKITVGAKVKPLDQTPVHGETGPK
CHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEEEECCEEEEECCEECCCCCCCCCCCCCCC
HDTDEAPDVPGDKAEVAPRAAAVQQRR
CCCCCCCCCCCCCHHHCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9827328 [H]