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The map label for this gene is nplT [H]

Identifier: 170750852

GI number: 170750852

Start: 4741962

End: 4743290

Strand: Reverse

Name: nplT [H]

Synonym: Mrad2831_4463

Alternate gene names: 170750852

Gene position: 4743290-4741962 (Counterclockwise)

Preceding gene: 170750855

Following gene: 170750849

Centisome position: 78.04

GC content: 74.57

Gene sequence:

>1329_bases
GTGGCGTCCTGGATCGAACAGACTGTCTGGTGGCAGGTCTTTCCCCTCGGCTTCGTCGGGGCCCCCCAGGAGGCCGAGCC
CGCCGCCCTGGTCACGCACCGCCTCTCCCACCTGCACGGCTGGCTCGACTACGCGGTCGAGCTCGGCGCGTCCGGGCTGG
CGCTCGGGCCGATCTTCGCGGCCTCCACGCACGGCTACGACACGGTCGACCATTTCCGCATCGACCCGCGCCTGGGCGAC
GAGGCCGATTTCGACGCGCTGATCGCGGCGGCCCGGGCCCGCGGCCTCCGGGTCCTGCTCGACGGCGTGTTCAACCATGT
CGGGCGGGCCCACCCCGCCTTCCAGGACGTCCTGCGGCACGGGCCCGGATCCGAGCGGGCCGGCTGGTTCAGGCTGTCCT
GGCCGCGGGGCTCCGGCCCGGGCATCGAGCCGGACTACGCCTGCTTCGAGGGGCACCGGCAGCTCGTCACGCTCAACCAC
GACGATCCGCGCGTCGCCGACCACGTCGCCGCCGTCATGGGCCACTGGATCGCCCGGGGCGCCGACGGCTGGCGGCTGGA
CGCGGCCTACGCGGTGCCGCCGCGCTTCTGGGCGCGCGTCCTGCGGCAGGTCCGCCTCGCGCATCCGGAGGCCTACGTTG
TCGGCGAGGTGATCCACGGCGATTACGCCCGGATCGTGCAGGACAGCGGCCTGGACGCGGTGACCCAGTACGAGCTGTGG
AAGGCGATCTGGAGCGCCCTCAACGATCGCAACCTGTTCGAGCTGGCTCACGCCCTGGACCGGCACAACGGCTATCTCGA
CCGGTTCGCGCCCCTGACCTTCGTGGGCAACCACGACGTGACGCGGATCGCGAGCCGGCTCACGGACGAGCGGCACCTGC
CGCACGCGCTGGCGATCCTGCTGACCTGCGGCGGGACGCCCTCGATCTACGCCGGCGACGAGCAGGCCTTCCGCGGCGTG
AAGGAGGAGCGGGTCGGCGGCGACGACGCCATCCGGCCGGCCTTCCCGCCGACGCCCGCGGATCTCGCCCCGTACGGCTG
GCCGGTCTACCGGCTGCACCAGGATCTGATCGCCCTCCGGCGGCGCCAGGGCGGGCTGCACCGCGCGCGGGCGCGGACGC
TGCACCTCGCCAAGGCAGAGCTCGTGCTCGCGCTGGGCGACGGGCGCGCGCGGGTGATGACGGCCCTGAACCTCGCCGAC
GCGGCCGCGACGCTGCCGGTGCCGGAGGCCCGGGCGATCGAGGCCGGCGGCGGCGACCTCGCCGAGGCCGGCGGCGCCGG
GGCCCGCGTGACCCTGCCGGCCCACGGCTGGGCCATCCTGAGCGTCTGA

Upstream 100 bases:

>100_bases
GGCCGCGGCCGGGGCGGCCAAGATCCCGCTCCTGCACCGGGGCCTGCAACCATCGCGGCACGACGGGGTTCGGTCCCGAT
TGGCAGTGGACGGAGGTCGG

Downstream 100 bases:

>100_bases
CCGTGCGGCGCACCGGGTCGGCGCACCGGGTCGGCGAAGGACCGGCCGCCGCCGGCCCCGAGCCCTAGCTGGTGCGGCGG
GCCGTCCAGCGCCCCGCGCA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MASWIEQTVWWQVFPLGFVGAPQEAEPAALVTHRLSHLHGWLDYAVELGASGLALGPIFAASTHGYDTVDHFRIDPRLGD
EADFDALIAAARARGLRVLLDGVFNHVGRAHPAFQDVLRHGPGSERAGWFRLSWPRGSGPGIEPDYACFEGHRQLVTLNH
DDPRVADHVAAVMGHWIARGADGWRLDAAYAVPPRFWARVLRQVRLAHPEAYVVGEVIHGDYARIVQDSGLDAVTQYELW
KAIWSALNDRNLFELAHALDRHNGYLDRFAPLTFVGNHDVTRIASRLTDERHLPHALAILLTCGGTPSIYAGDEQAFRGV
KEERVGGDDAIRPAFPPTPADLAPYGWPVYRLHQDLIALRRRQGGLHRARARTLHLAKAELVLALGDGRARVMTALNLAD
AAATLPVPEARAIEAGGGDLAEAGGAGARVTLPAHGWAILSV

Sequences:

>Translated_442_residues
MASWIEQTVWWQVFPLGFVGAPQEAEPAALVTHRLSHLHGWLDYAVELGASGLALGPIFAASTHGYDTVDHFRIDPRLGD
EADFDALIAAARARGLRVLLDGVFNHVGRAHPAFQDVLRHGPGSERAGWFRLSWPRGSGPGIEPDYACFEGHRQLVTLNH
DDPRVADHVAAVMGHWIARGADGWRLDAAYAVPPRFWARVLRQVRLAHPEAYVVGEVIHGDYARIVQDSGLDAVTQYELW
KAIWSALNDRNLFELAHALDRHNGYLDRFAPLTFVGNHDVTRIASRLTDERHLPHALAILLTCGGTPSIYAGDEQAFRGV
KEERVGGDDAIRPAFPPTPADLAPYGWPVYRLHQDLIALRRRQGGLHRARARTLHLAKAELVLALGDGRARVMTALNLAD
AAATLPVPEARAIEAGGGDLAEAGGAGARVTLPAHGWAILSV
>Mature_441_residues
ASWIEQTVWWQVFPLGFVGAPQEAEPAALVTHRLSHLHGWLDYAVELGASGLALGPIFAASTHGYDTVDHFRIDPRLGDE
ADFDALIAAARARGLRVLLDGVFNHVGRAHPAFQDVLRHGPGSERAGWFRLSWPRGSGPGIEPDYACFEGHRQLVTLNHD
DPRVADHVAAVMGHWIARGADGWRLDAAYAVPPRFWARVLRQVRLAHPEAYVVGEVIHGDYARIVQDSGLDAVTQYELWK
AIWSALNDRNLFELAHALDRHNGYLDRFAPLTFVGNHDVTRIASRLTDERHLPHALAILLTCGGTPSIYAGDEQAFRGVK
EERVGGDDAIRPAFPPTPADLAPYGWPVYRLHQDLIALRRRQGGLHRARARTLHLAKAELVLALGDGRARVMTALNLADA
AATLPVPEARAIEAGGGDLAEAGGAGARVTLPAHGWAILSV

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=399, Percent_Identity=26.0651629072682, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790687, Length=372, Percent_Identity=26.8817204301075, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 48245; Mature: 48114

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASWIEQTVWWQVFPLGFVGAPQEAEPAALVTHRLSHLHGWLDYAVELGASGLALGPIFA
CCHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHE
ASTHGYDTVDHFRIDPRLGDEADFDALIAAARARGLRVLLDGVFNHVGRAHPAFQDVLRH
ECCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHC
GPGSERAGWFRLSWPRGSGPGIEPDYACFEGHRQLVTLNHDDPRVADHVAAVMGHWIARG
CCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
ADGWRLDAAYAVPPRFWARVLRQVRLAHPEAYVVGEVIHGDYARIVQDSGLDAVTQYELW
CCCCEECCEEECCHHHHHHHHHHHHHCCCCCEEEHHHHCCCHHHHHHCCCCCHHHHHHHH
KAIWSALNDRNLFELAHALDRHNGYLDRFAPLTFVGNHDVTRIASRLTDERHLPHALAIL
HHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHH
LTCGGTPSIYAGDEQAFRGVKEERVGGDDAIRPAFPPTPADLAPYGWPVYRLHQDLIALR
EECCCCCCEECCCHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RRQGGLHRARARTLHLAKAELVLALGDGRARVMTALNLADAAATLPVPEARAIEAGGGDL
HHCCCHHHHHHHHHEEEEEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCEEECCCCCH
AEAGGAGARVTLPAHGWAILSV
HHCCCCCCEEEECCCCEEEEEC
>Mature Secondary Structure 
ASWIEQTVWWQVFPLGFVGAPQEAEPAALVTHRLSHLHGWLDYAVELGASGLALGPIFA
CHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHE
ASTHGYDTVDHFRIDPRLGDEADFDALIAAARARGLRVLLDGVFNHVGRAHPAFQDVLRH
ECCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHC
GPGSERAGWFRLSWPRGSGPGIEPDYACFEGHRQLVTLNHDDPRVADHVAAVMGHWIARG
CCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
ADGWRLDAAYAVPPRFWARVLRQVRLAHPEAYVVGEVIHGDYARIVQDSGLDAVTQYELW
CCCCEECCEEECCHHHHHHHHHHHHHCCCCCEEEHHHHCCCHHHHHHCCCCCHHHHHHHH
KAIWSALNDRNLFELAHALDRHNGYLDRFAPLTFVGNHDVTRIASRLTDERHLPHALAIL
HHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHH
LTCGGTPSIYAGDEQAFRGVKEERVGGDDAIRPAFPPTPADLAPYGWPVYRLHQDLIALR
EECCCCCCEECCCHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RRQGGLHRARARTLHLAKAELVLALGDGRARVMTALNLADAAATLPVPEARAIEAGGGDL
HHCCCHHHHHHHHHEEEEEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCEEECCCCCH
AEAGGAGARVTLPAHGWAILSV
HHCCCCCCEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]