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The map label for this gene is yfcG [H]

Identifier: 170750826

GI number: 170750826

Start: 4718573

End: 4719193

Strand: Direct

Name: yfcG [H]

Synonym: Mrad2831_4437

Alternate gene names: 170750826

Gene position: 4718573-4719193 (Clockwise)

Preceding gene: 170750825

Following gene: 170750827

Centisome position: 77.64

GC content: 71.34

Gene sequence:

>621_bases
ATGATCGATCTGTACGCCTGGAACACCCCCAACGGCCGGAAGATCAGCGTGGCGCTGGAGGAGATGGCGCTGCCCTACCG
GGTCATCCCGGTCGACATCACCCAGGGCGCGCAGCACGCGCCGGACTTCCTGGCGATCAGCCCGAACAACCGCATCCCCG
CCATCGTCGATCCGGACGGGCCGGACGGGTCGCCGATCCCGGTCTTCGAGTCCGGCGCGATCCTGCTCTACCTCGCCGAG
AAGACCGGCCGCTTCCTGCCCGAGGACCGGCGGCAGCGCGTCGCCGCCCTGGAATGGCTGATGTGGCAGATGGGCGGCTT
CGGGCCCATGCCCGGCCAGGTTCACCACTTCCTGACCGTCGCCGAGGCCGACCGCGCCTACGGGCTCGCCCGCTACAGCA
CGGAGACGCACCGGCTCTACGGCGTGCTGGACCGGCGCCTCGCGCAGGTCGCGTACGTCGCGGGACCGCTCTCGGTGGCG
GATTTCGCGATCCTCGGCTGGGCGTGGCGCCACGAGCGCCATCAGGTCGACCTCGCCGACTACCCGCAGGTCCGGCGCTG
GTACGACCGGATGATGGCGCGCCCCGCCGTGCAGCGGGGCTTCGCCGTCGCGCTGAGCTGA

Upstream 100 bases:

>100_bases
CCGGAGCCCGAGGCGGCCGCGGTCGCGGCCTGAGCGCCGGCCGAATTGCCGGACCCCGCGGCCGGCCCTAGCTTCCCTCC
GCACGGCGGAGGCGCCCGCG

Downstream 100 bases:

>100_bases
GGCGGGGCGTCCCTGGTCCGGCGCTTGCGACGCGGAGGTCCACCGTTCGGGGAGTGACGATGAGGCGACGCACCTTCCTC
GCCGCCGGGGCCGCGGCCCT

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MIDLYAWNTPNGRKISVALEEMALPYRVIPVDITQGAQHAPDFLAISPNNRIPAIVDPDGPDGSPIPVFESGAILLYLAE
KTGRFLPEDRRQRVAALEWLMWQMGGFGPMPGQVHHFLTVAEADRAYGLARYSTETHRLYGVLDRRLAQVAYVAGPLSVA
DFAILGWAWRHERHQVDLADYPQVRRWYDRMMARPAVQRGFAVALS

Sequences:

>Translated_206_residues
MIDLYAWNTPNGRKISVALEEMALPYRVIPVDITQGAQHAPDFLAISPNNRIPAIVDPDGPDGSPIPVFESGAILLYLAE
KTGRFLPEDRRQRVAALEWLMWQMGGFGPMPGQVHHFLTVAEADRAYGLARYSTETHRLYGVLDRRLAQVAYVAGPLSVA
DFAILGWAWRHERHQVDLADYPQVRRWYDRMMARPAVQRGFAVALS
>Mature_206_residues
MIDLYAWNTPNGRKISVALEEMALPYRVIPVDITQGAQHAPDFLAISPNNRIPAIVDPDGPDGSPIPVFESGAILLYLAE
KTGRFLPEDRRQRVAALEWLMWQMGGFGPMPGQVHHFLTVAEADRAYGLARYSTETHRLYGVLDRRLAQVAYVAGPLSVA
DFAILGWAWRHERHQVDLADYPQVRRWYDRMMARPAVQRGFAVALS

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=206, Percent_Identity=51.4563106796116, Blast_Score=202, Evalue=9e-54,
Organism=Escherichia coli, GI87082195, Length=215, Percent_Identity=37.6744186046512, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI87081789, Length=207, Percent_Identity=29.4685990338164, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324100, Length=228, Percent_Identity=32.0175438596491, Blast_Score=108, Evalue=8e-25,
Organism=Drosophila melanogaster, GI17864592, Length=183, Percent_Identity=28.9617486338798, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571670, Length=195, Percent_Identity=27.1794871794872, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17864594, Length=189, Percent_Identity=29.1005291005291, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17933730, Length=192, Percent_Identity=27.6041666666667, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864596, Length=192, Percent_Identity=27.6041666666667, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 23209; Mature: 23209

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYAWNTPNGRKISVALEEMALPYRVIPVDITQGAQHAPDFLAISPNNRIPAIVDPDG
CEEEEEECCCCCCEEEEEEHHHCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEECCCC
PDGSPIPVFESGAILLYLAEKTGRFLPEDRRQRVAALEWLMWQMGGFGPMPGQVHHFLTV
CCCCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEE
AEADRAYGLARYSTETHRLYGVLDRRLAQVAYVAGPLSVADFAILGWAWRHERHQVDLAD
ECCHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCC
YPQVRRWYDRMMARPAVQRGFAVALS
CHHHHHHHHHHHHCHHHHCCEEEEEC
>Mature Secondary Structure
MIDLYAWNTPNGRKISVALEEMALPYRVIPVDITQGAQHAPDFLAISPNNRIPAIVDPDG
CEEEEEECCCCCCEEEEEEHHHCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEECCCC
PDGSPIPVFESGAILLYLAEKTGRFLPEDRRQRVAALEWLMWQMGGFGPMPGQVHHFLTV
CCCCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEE
AEADRAYGLARYSTETHRLYGVLDRRLAQVAYVAGPLSVADFAILGWAWRHERHQVDLAD
ECCHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCC
YPQVRRWYDRMMARPAVQRGFAVALS
CHHHHHHHHHHHHCHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]