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The map label for this gene is ruvB [H]

Identifier: 170750825

GI number: 170750825

Start: 4717471

End: 4718505

Strand: Direct

Name: ruvB [H]

Synonym: Mrad2831_4436

Alternate gene names: 170750825

Gene position: 4717471-4718505 (Clockwise)

Preceding gene: 170750824

Following gene: 170750826

Centisome position: 77.62

GC content: 72.17

Gene sequence:

>1035_bases
ATGGCGGCGCCGATCAAGACGGCCCGCCCGGGACCGGTCCCGGGCGCGGAGGCGACCGCCGCGGAGGCGGATCGGACGAT
CCGCCCTCTGAGCCTGTCGGAGTTCATCGGTCAGCGTGCGGCGCGTGCGAACATGCAGGTGTTCATCGAGGCTGCGAAGA
AGACCGGTCAGGCGCTCGACCACGTGCTGTTCGTCGGTCCGCCGGGGCTGGGCAAGACGACGCTGGCCCAGATCGTGGCG
CGCGAGCTGGGCGTGAACTTCCGCTCGACCTCCGGGCCGGTGATCGCCAAGGCCGGCGACCTGGCCGCGCAGCTCACCAA
CCTGGAGGAGCGCGACGTCCTGTTCATCGACGAGATCCACCGGCTCAACCCGGCGGTCGAGGAGATCCTCTACCCGGCCA
TGGAGGATTACCAGCTCGACCTGATCATCGGCGAGGGCCCGGCGGCGCGCTCGGTCAAGATCGAGCTGCCGAAATTCACC
CTGGTCGGCGCCACGACGCGGGCGGGGCTGCTGACGACGCCGCTGCGCGACCGGTTCGGGATCCCGATCCGGCTGGAGTT
CTACGAGATCGACGAGCTGGAGCAGATCGTGGCGCGCGGCGCGCGGGTCCTGGGCTTGGGCATGTCGGCGGAGGGCGCCA
ACGAGATCGCCCGGCGGGCGCGGGGCACGCCGCGGATCGCGGGGCGGCTGTTGCGGCGGGTGCGGGATTTCGCGGTCGTG
GCGGAGGCCGAGACGGTGACGCGGGCGATCGCCGACCGGGCGCTGCAGCTCCTGGACGTGGACGGGGCGGGTCTGGACGT
GATGGACCGCAAGTACCTGAGCCTGATCGCGCGCGCGTTCGGGGGCGGCCCGGTGGGGATCGAGACCATCGGCGCGGCCT
TGTCGGAGCCGCGCGACGCCATCGAGGACATCATCGAGCCCTACCTGATCCAGCGCGGCTTCGTGCAGCGCACGCCCCGC
GGCCGCGTCCTCACACGACACGCGTACCGGCATCTCGGCCTGCCGGAGCCCGAGGCGGCCGCGGTCGCGGCCTGA

Upstream 100 bases:

>100_bases
GTGGCGGGTGAGCGTGCACGACCTCTCGGGCCGGCGGGTCCTGGTGCAGCCCTTCGTGCAGCCCTTCGAGAAGGCGTCGG
GTCGCGCCAGGGCGGCCTGA

Downstream 100 bases:

>100_bases
GCGCCGGCCGAATTGCCGGACCCCGCGGCCGGCCCTAGCTTCCCTCCGCACGGCGGAGGCGCCCGCGATGATCGATCTGT
ACGCCTGGAACACCCCCAAC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAAPIKTARPGPVPGAEATAAEADRTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVA
RELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFT
LVGATTRAGLLTTPLRDRFGIPIRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVV
AEAETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDAIEDIIEPYLIQRGFVQRTPR
GRVLTRHAYRHLGLPEPEAAAVAA

Sequences:

>Translated_344_residues
MAAPIKTARPGPVPGAEATAAEADRTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVA
RELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFT
LVGATTRAGLLTTPLRDRFGIPIRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVV
AEAETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDAIEDIIEPYLIQRGFVQRTPR
GRVLTRHAYRHLGLPEPEAAAVAA
>Mature_343_residues
AAPIKTARPGPVPGAEATAAEADRTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALDHVLFVGPPGLGKTTLAQIVAR
ELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTL
VGATTRAGLLTTPLRDRFGIPIRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVVA
EAETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDAIEDIIEPYLIQRGFVQRTPRG
RVLTRHAYRHLGLPEPEAAAVAA

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=316, Percent_Identity=65.5063291139241, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1787119, Length=146, Percent_Identity=33.5616438356164, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37067; Mature: 36936

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPIKTARPGPVPGAEATAAEADRTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALD
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVLFVGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIH
CEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCEEEECHHHHHHHHCCCCCCEEHHHHHH
RLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLVGATTRAGLLTTPLRDRFG
HCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCEEECCHHHHCC
IPIRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVV
CCEEEEEECHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AEAETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDA
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHH
IEDIIEPYLIQRGFVQRTPRGRVLTRHAYRHLGLPEPEAAAVAA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
AAPIKTARPGPVPGAEATAAEADRTIRPLSLSEFIGQRAARANMQVFIEAAKKTGQALD
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVLFVGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAAQLTNLEERDVLFIDEIH
CEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCEEEECHHHHHHHHCCCCCCEEHHHHHH
RLNPAVEEILYPAMEDYQLDLIIGEGPAARSVKIELPKFTLVGATTRAGLLTTPLRDRFG
HCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCEEECCHHHHCC
IPIRLEFYEIDELEQIVARGARVLGLGMSAEGANEIARRARGTPRIAGRLLRRVRDFAVV
CCEEEEEECHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AEAETVTRAIADRALQLLDVDGAGLDVMDRKYLSLIARAFGGGPVGIETIGAALSEPRDA
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHH
IEDIIEPYLIQRGFVQRTPRGRVLTRHAYRHLGLPEPEAAAVAA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]