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The map label for this gene is gcvA [H]

Identifier: 170750804

GI number: 170750804

Start: 4692037

End: 4692924

Strand: Reverse

Name: gcvA [H]

Synonym: Mrad2831_4415

Alternate gene names: 170750804

Gene position: 4692924-4692037 (Counterclockwise)

Preceding gene: 170750806

Following gene: 170750803

Centisome position: 77.21

GC content: 76.58

Gene sequence:

>888_bases
GTGCATCGTCGTTTGCCGCCGCTGAACGCGCTGAAAGCCTTCGAGGCGGCCGCCCGGCACGGCAGCTTCACGCGCGCGGC
GGACGAGCTGCGGGTCACCCACGGGGCGGTCAGCCGGCACGTGCAGACGCTGGAGGGCTGGCTCGGGCTGCCGCTGTTCG
AGCGCCACAACCGGCGCGTGGTCCTCACCGAGGCGGGCAGCGCCTACGCCACCGAGATCGGCGCCGCCCTCGACCGCATC
GCCCTGGCGACGGCGCGCCAGGCCGAGCGCGGCCGGCCGCGCCTGCTGCGCGTGAACGCGCTCGCCACCTTCACCCTGCG
CTGGCTGATCCCGCGCCTGTCGGGCTTCCAGCGGGCCAACCCGTCGATCGAGGTGCGGCTGACGACCTCGAACGTGCCCC
TCGCCAGCGTGGCCGAGCCGTTCGACGTGGCGATCCGGGGCGGCCCGGACAGCCGCCCGGGCCACCGCGCGGCGCCGTTC
CTGTCCGAGCGGCGCATTCCCGTCTGCAGCCCGGACCTGCTGCGGCGGCTGCCGCTCCGGGTGCCGGAGGATCTCCGGCA
GCACACGCTCCTGCACGCCGCGACGCTGCCGCGGGTCTGGCCCGACTGGCTGCGGGCGGCGGGCGTCCCGGACCTGGCGC
CGCGGGCCACGGTGACGCTGGAACACTTCTACCTGACCCTTCAGGCCGCCCTGGACGGGCTCGGCGTCGCCATGGGGCCC
GAGCGGCTGATCGCCGACGACGTCGCGGCCGGGCGCCTGACCCTGCCCTTCGCTGGGCCGGCCCTGCCGGCCCGGAGCTA
CTACGCCTACGTCCCGGAACCCCGGGCGGAGGACCCGGCGGTGCGGGCCTTCTGCGGCTGGCTCGCCGAGGCCGCGCGGC
AGCCGTGA

Upstream 100 bases:

>100_bases
CGGACATCCAGCCATCCTTCGTGGTTCCGTCACAGGATGGCCGCCTGGTCCGTCGCTGACAAACGACTTATGCTGACACT
CGTGTGAGGAAACCTGACCC

Downstream 100 bases:

>100_bases
GCGCCGCTGTCGGACGGCGCGCCGCCCGGATGGCTTCGCGGCGCGCGCGATGACGATCTCGGCTTGGCGGCCGCCGCCGT
CCGGCGCATGGATCGGATCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MHRRLPPLNALKAFEAAARHGSFTRAADELRVTHGAVSRHVQTLEGWLGLPLFERHNRRVVLTEAGSAYATEIGAALDRI
ALATARQAERGRPRLLRVNALATFTLRWLIPRLSGFQRANPSIEVRLTTSNVPLASVAEPFDVAIRGGPDSRPGHRAAPF
LSERRIPVCSPDLLRRLPLRVPEDLRQHTLLHAATLPRVWPDWLRAAGVPDLAPRATVTLEHFYLTLQAALDGLGVAMGP
ERLIADDVAAGRLTLPFAGPALPARSYYAYVPEPRAEDPAVRAFCGWLAEAARQP

Sequences:

>Translated_295_residues
MHRRLPPLNALKAFEAAARHGSFTRAADELRVTHGAVSRHVQTLEGWLGLPLFERHNRRVVLTEAGSAYATEIGAALDRI
ALATARQAERGRPRLLRVNALATFTLRWLIPRLSGFQRANPSIEVRLTTSNVPLASVAEPFDVAIRGGPDSRPGHRAAPF
LSERRIPVCSPDLLRRLPLRVPEDLRQHTLLHAATLPRVWPDWLRAAGVPDLAPRATVTLEHFYLTLQAALDGLGVAMGP
ERLIADDVAAGRLTLPFAGPALPARSYYAYVPEPRAEDPAVRAFCGWLAEAARQP
>Mature_295_residues
MHRRLPPLNALKAFEAAARHGSFTRAADELRVTHGAVSRHVQTLEGWLGLPLFERHNRRVVLTEAGSAYATEIGAALDRI
ALATARQAERGRPRLLRVNALATFTLRWLIPRLSGFQRANPSIEVRLTTSNVPLASVAEPFDVAIRGGPDSRPGHRAAPF
LSERRIPVCSPDLLRRLPLRVPEDLRQHTLLHAATLPRVWPDWLRAAGVPDLAPRATVTLEHFYLTLQAALDGLGVAMGP
ERLIADDVAAGRLTLPFAGPALPARSYYAYVPEPRAEDPAVRAFCGWLAEAARQP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=41.156462585034, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=32.2916666666667, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=29.4915254237288, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=30.952380952381, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=28.2894736842105, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787128, Length=285, Percent_Identity=27.0175438596491, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=163, Percent_Identity=34.3558282208589, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787589, Length=298, Percent_Identity=28.1879194630872, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081978, Length=284, Percent_Identity=27.4647887323944, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=259, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32401; Mature: 32401

Theoretical pI: Translated: 11.18; Mature: 11.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRRLPPLNALKAFEAAARHGSFTRAADELRVTHGAVSRHVQTLEGWLGLPLFERHNRRV
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEE
VLTEAGSAYATEIGAALDRIALATARQAERGRPRLLRVNALATFTLRWLIPRLSGFQRAN
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCC
PSIEVRLTTSNVPLASVAEPFDVAIRGGPDSRPGHRAAPFLSERRIPVCSPDLLRRLPLR
CCEEEEEEECCCCHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCCHHHHHHCCCC
VPEDLRQHTLLHAATLPRVWPDWLRAAGVPDLAPRATVTLEHFYLTLQAALDGLGVAMGP
CCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCC
ERLIADDVAAGRLTLPFAGPALPARSYYAYVPEPRAEDPAVRAFCGWLAEAARQP
HHHHHHHCCCCEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHRRLPPLNALKAFEAAARHGSFTRAADELRVTHGAVSRHVQTLEGWLGLPLFERHNRRV
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEE
VLTEAGSAYATEIGAALDRIALATARQAERGRPRLLRVNALATFTLRWLIPRLSGFQRAN
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCC
PSIEVRLTTSNVPLASVAEPFDVAIRGGPDSRPGHRAAPFLSERRIPVCSPDLLRRLPLR
CCEEEEEEECCCCHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCCHHHHHHCCCC
VPEDLRQHTLLHAATLPRVWPDWLRAAGVPDLAPRATVTLEHFYLTLQAALDGLGVAMGP
CCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCC
ERLIADDVAAGRLTLPFAGPALPARSYYAYVPEPRAEDPAVRAFCGWLAEAARQP
HHHHHHHCCCCEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]