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The map label for this gene is amaB [H]

Identifier: 170750780

GI number: 170750780

Start: 4660397

End: 4661683

Strand: Direct

Name: amaB [H]

Synonym: Mrad2831_4391

Alternate gene names: 170750780

Gene position: 4660397-4661683 (Clockwise)

Preceding gene: 170750778

Following gene: 170750781

Centisome position: 76.68

GC content: 75.84

Gene sequence:

>1287_bases
ATGGACCGCACGGTGGACCGCATCAATCTCCCGCTCTCGCAGGACCGGCTCTGGGCCAGCCTGATGGACCTCGCCCGGAT
CGGCGCGCTGCCCAACGGCGGCAACGACCGGCAGGCCCTGACCGACCGCGACGCCGAGGGGCGCGCCCTGTTCCGGCGCT
GGGGCGAGGCGGTGGGGCTGACGCTCACCGTGGACCGGGTCGGCACCATGATCTTCCGCCGTCCCGGGCGGGATCCGAGC
CGCAGGCCCGTGGCGATCGGCAGCCACCTCGACACCCAGCCCACCGGGGGCAAGTTCGACGGGCCGCTCGGCGTGCTCGC
CGGGCTGGAGATCATGCGCGCCCTGCACGAGGCCGGGATCGAGACCGAGGCGCCGCTCCTGCTGATCAACTGGTGCAACG
AGGAGGGCGCGCGCTTCGCGCCGCCGATGAGCGGCAGCGGCGTCGCCATGGGCATCGTCCCGGAGGCCGACATCCTGGCG
ACCCGGGACCTCGCGGGCCACGCCTTCGGCGACGAACTGCGGCGCATCGGCTGGCAGGGGACGGCGGACCCGGCCGCCCT
GCGGGAGATCGGCGCCTATTTCGAGCTCCACATCGAGCAGGGCAAGCGCCTGGAGGATGCGGGCCTCACCATCGGGGTGG
TCGACCGGGCGCTCGCCCAGATCTGGTACGAGATCACGGTGCTCGGCGAGGAGGCCCATGCCGGCAGCCCCATGGCGGGC
CGGCGCGACGCGATGATGGCCGCGGCGGCGCTGATCGCGTCGCTGGAGGGCATCGCGGAGGCGGCGGTCGGCGCGGGCGG
GGAGCGCGGCCGCGCCACCGTCGGCGTGCTGCGGGCGGAGCCGGCGAGCCGCAACATCACGCCCGGCCGGGTCTGGTTCA
GCCTCGACACCCGCCACGGCGACACCGCGCAGCTGGAGGCGATGGGCGCGCAGATCCGGGCCCGGGCCGACGCCCTGGCG
GCGGAGCGCGGCGTCGCCGTCCAGGTCGCGGAGTTCTGGCGGGCGCCGCCGACCCCGTTCGACCCGGTCCTGGTGGACCG
CGTCCAGGCGGCGGCCGAGGCGCGGGGCCATGCCTGGACGCGGATGCCGACCGCGATCTACCACGACGCCGTCTACTGCG
CCCGCGCGGTGCCGGCGGCCCTGGTGTTCTGCCCCTGCCACGGCGGGATCAGCCACAACGAGGCCGAGAGCATCACGCCG
GGCTGGGCCGGGGCCGGTCTGGAGGTCCTGGCCGACGCGGTGCTGGCGACGGCGGGCCTCGCCGGTCCTCGGGCCGGGCA
GGCCTGA

Upstream 100 bases:

>100_bases
CGGGGACGCGCGCCGGGCCTGCCCTGGTGCGGGTTGCCGGTGCGCCGTAACCTGCACCTTCCGGTAAGCATTCGGCCGAG
AGGCCCGCAGCAGAGACGCC

Downstream 100 bases:

>100_bases
GCGCGGCGCCGCCGAGCGCGCACGAACGGAGACTGGGTTCTCGCATGTCCGACGACAACGTCATCCGCCCCGCCTTCGGC
GTCCCCCGCAATCCGGCGCC

Product: amidase

Products: NA

Alternate protein names: L-carbamoylase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MDRTVDRINLPLSQDRLWASLMDLARIGALPNGGNDRQALTDRDAEGRALFRRWGEAVGLTLTVDRVGTMIFRRPGRDPS
RRPVAIGSHLDTQPTGGKFDGPLGVLAGLEIMRALHEAGIETEAPLLLINWCNEEGARFAPPMSGSGVAMGIVPEADILA
TRDLAGHAFGDELRRIGWQGTADPAALREIGAYFELHIEQGKRLEDAGLTIGVVDRALAQIWYEITVLGEEAHAGSPMAG
RRDAMMAAAALIASLEGIAEAAVGAGGERGRATVGVLRAEPASRNITPGRVWFSLDTRHGDTAQLEAMGAQIRARADALA
AERGVAVQVAEFWRAPPTPFDPVLVDRVQAAAEARGHAWTRMPTAIYHDAVYCARAVPAALVFCPCHGGISHNEAESITP
GWAGAGLEVLADAVLATAGLAGPRAGQA

Sequences:

>Translated_428_residues
MDRTVDRINLPLSQDRLWASLMDLARIGALPNGGNDRQALTDRDAEGRALFRRWGEAVGLTLTVDRVGTMIFRRPGRDPS
RRPVAIGSHLDTQPTGGKFDGPLGVLAGLEIMRALHEAGIETEAPLLLINWCNEEGARFAPPMSGSGVAMGIVPEADILA
TRDLAGHAFGDELRRIGWQGTADPAALREIGAYFELHIEQGKRLEDAGLTIGVVDRALAQIWYEITVLGEEAHAGSPMAG
RRDAMMAAAALIASLEGIAEAAVGAGGERGRATVGVLRAEPASRNITPGRVWFSLDTRHGDTAQLEAMGAQIRARADALA
AERGVAVQVAEFWRAPPTPFDPVLVDRVQAAAEARGHAWTRMPTAIYHDAVYCARAVPAALVFCPCHGGISHNEAESITP
GWAGAGLEVLADAVLATAGLAGPRAGQA
>Mature_428_residues
MDRTVDRINLPLSQDRLWASLMDLARIGALPNGGNDRQALTDRDAEGRALFRRWGEAVGLTLTVDRVGTMIFRRPGRDPS
RRPVAIGSHLDTQPTGGKFDGPLGVLAGLEIMRALHEAGIETEAPLLLINWCNEEGARFAPPMSGSGVAMGIVPEADILA
TRDLAGHAFGDELRRIGWQGTADPAALREIGAYFELHIEQGKRLEDAGLTIGVVDRALAQIWYEITVLGEEAHAGSPMAG
RRDAMMAAAALIASLEGIAEAAVGAGGERGRATVGVLRAEPASRNITPGRVWFSLDTRHGDTAQLEAMGAQIRARADALA
AERGVAVQVAEFWRAPPTPFDPVLVDRVQAAAEARGHAWTRMPTAIYHDAVYCARAVPAALVFCPCHGGISHNEAESITP
GWAGAGLEVLADAVLATAGLAGPRAGQA

Specific function: Involved In The Anaerobic Utilization Of Allantoin. Converts Allantoate To (S)-Ureidoglycolate And Ammonia. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=393, Percent_Identity=30.5343511450382, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.87 [H]

Molecular weight: Translated: 45285; Mature: 45285

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00200 RIESKE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRTVDRINLPLSQDRLWASLMDLARIGALPNGGNDRQALTDRDAEGRALFRRWGEAVGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCE
TLTVDRVGTMIFRRPGRDPSRRPVAIGSHLDTQPTGGKFDGPLGVLAGLEIMRALHEAGI
EEEHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
ETEAPLLLINWCNEEGARFAPPMSGSGVAMGIVPEADILATRDLAGHAFGDELRRIGWQG
CCCCCEEEEEECCCCCCEECCCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCC
TADPAALREIGAYFELHIEQGKRLEDAGLTIGVVDRALAQIWYEITVLGEEAHAGSPMAG
CCCHHHHHHHHHHEEEEECCCCEEHHCCCEEHHHHHHHHHHHHHEEEEECCCCCCCCCCC
RRDAMMAAAALIASLEGIAEAAVGAGGERGRATVGVLRAEPASRNITPGRVWFSLDTRHG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCC
DTAQLEAMGAQIRARADALAAERGVAVQVAEFWRAPPTPFDPVLVDRVQAAAEARGHAWT
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHH
RMPTAIYHDAVYCARAVPAALVFCPCHGGISHNEAESITPGWAGAGLEVLADAVLATAGL
HCCHHHHHHHHHHHHHHCHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
AGPRAGQA
CCCCCCCC
>Mature Secondary Structure
MDRTVDRINLPLSQDRLWASLMDLARIGALPNGGNDRQALTDRDAEGRALFRRWGEAVGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCE
TLTVDRVGTMIFRRPGRDPSRRPVAIGSHLDTQPTGGKFDGPLGVLAGLEIMRALHEAGI
EEEHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
ETEAPLLLINWCNEEGARFAPPMSGSGVAMGIVPEADILATRDLAGHAFGDELRRIGWQG
CCCCCEEEEEECCCCCCEECCCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCC
TADPAALREIGAYFELHIEQGKRLEDAGLTIGVVDRALAQIWYEITVLGEEAHAGSPMAG
CCCHHHHHHHHHHEEEEECCCCEEHHCCCEEHHHHHHHHHHHHHEEEEECCCCCCCCCCC
RRDAMMAAAALIASLEGIAEAAVGAGGERGRATVGVLRAEPASRNITPGRVWFSLDTRHG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCC
DTAQLEAMGAQIRARADALAAERGVAVQVAEFWRAPPTPFDPVLVDRVQAAAEARGHAWT
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHH
RMPTAIYHDAVYCARAVPAALVFCPCHGGISHNEAESITPGWAGAGLEVLADAVLATAGL
HCCHHHHHHHHHHHHHHCHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
AGPRAGQA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9023955; 8285691 [H]