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The map label for this gene is yegE [H]

Identifier: 170750733

GI number: 170750733

Start: 4602782

End: 4603891

Strand: Reverse

Name: yegE [H]

Synonym: Mrad2831_4344

Alternate gene names: 170750733

Gene position: 4603891-4602782 (Counterclockwise)

Preceding gene: 170750736

Following gene: 170750731

Centisome position: 75.75

GC content: 78.2

Gene sequence:

>1110_bases
ATGACGACCGCGCGTTCCCTGGCCCTGGTGAAATCGCCCCGCGACCGATCCGTCCGCGGGCGCGCCGACCCGTCGGCCGC
GGACGAGGCGGTCGCCGAGGCCGCGGCCGCCTGCGCCCTGCAGCGGGCGATCATCCGCCAGCAGGCCGGCCTGCTGGCGC
GGCAGGAGCGGGTCCTGGCGGACGCCGGGATGGGCCTGTGGTCCTGCCGCCTGCACGACGGTGCCCTCGACTGGTCCGGC
GGCGTCCACGACCTGTTCGGCGTTCCCCGAGGCGCGGCCCTGCATCGGCCCCGGATCCTGGACCTCTACGAGCCGGCCTC
GCGGCGCCTGCTGGAGACGCTCCGGCACCGGGCGATCGCCCGGGGCGGCGCCTTCGCGCTCGACGCCGAGGTGCTGGCCG
GAGGCCGGCGACGCTGGATCCGGATCCGCGCCGCGGTCGAGTCCGAGGACGGGCGCCCGACCCGCCTCTTCGGAACCAAG
CAGGACGTCACCGAGGAGATCGGCCGGCTGGCCGAGCTGAGCCGGCGCGCCGAGTGCGACCCGCTGACCGGGCTGGCGAA
CCGCCGCGGCTTCGACGCGCAGTTCGCGGCGCAGTGCGCGGCACAGTTCCGGACCGGGACGGCCGGCTCCGGTCCGGAGC
CGCTCGGCGCCCTGCTGCTGATCGACCTCGACGGGTTCAAGCCGGTCAACGACATCCACGGCCACGCGGCCGGCGACCTG
TGCCTCAGGCGCGCGGCCGCGCGGCTGGCCGCCGTCTGCCGGGACGCGACCCTCGTGGCCCGCATCGGCGGCGACGAGTT
CGCCGTGCTCCTGCGGGCGCCCGTCGACCCGTCCGGGCTGGTCGAGCGCGGACGGCGGATCGTGGCCGCCTTGAGCCGAC
CGGTCCCGTATCGCGGCCACGGGCTGCGGATCGGTGCCTCCGTGGGGATCGCCGTGGGGACGGCCGCGGGGACGGCCGCG
GGCGGCGGCCCCGCCCGGCTGTTCGGGCGGGCGGACGCGGCGCTCTACGCCGCCAAGGACGCGGGCGGGAACACCGTCCG
GATCGCCCGCGGCGGCGGCCGGTCAGGCGCCGGCGCTGCGCAGACCGCGGTCGCGCGGGGGCCGGGCTGA

Upstream 100 bases:

>100_bases
GACGGCCCTGCCGCCCGGGCGGCATATAGGCGTTGCCGGGCGGCCGCGCATCGGACGATTAGGGCTTCCTCAAAGGCCTT
GGGTCTAAAACCGCACACCG

Downstream 100 bases:

>100_bases
TTCGCCACCCTCGGGGAGCGCGGCCGGCTGGATGAAGCGGCTGGCGATCTCGCCCATGGCGCTCTGCATCGTGTCGAGGC
CCTGCACGGTGACGATGCTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTTARSLALVKSPRDRSVRGRADPSAADEAVAEAAAACALQRAIIRQQAGLLARQERVLADAGMGLWSCRLHDGALDWSG
GVHDLFGVPRGAALHRPRILDLYEPASRRLLETLRHRAIARGGAFALDAEVLAGGRRRWIRIRAAVESEDGRPTRLFGTK
QDVTEEIGRLAELSRRAECDPLTGLANRRGFDAQFAAQCAAQFRTGTAGSGPEPLGALLLIDLDGFKPVNDIHGHAAGDL
CLRRAAARLAAVCRDATLVARIGGDEFAVLLRAPVDPSGLVERGRRIVAALSRPVPYRGHGLRIGASVGIAVGTAAGTAA
GGGPARLFGRADAALYAAKDAGGNTVRIARGGGRSGAGAAQTAVARGPG

Sequences:

>Translated_369_residues
MTTARSLALVKSPRDRSVRGRADPSAADEAVAEAAAACALQRAIIRQQAGLLARQERVLADAGMGLWSCRLHDGALDWSG
GVHDLFGVPRGAALHRPRILDLYEPASRRLLETLRHRAIARGGAFALDAEVLAGGRRRWIRIRAAVESEDGRPTRLFGTK
QDVTEEIGRLAELSRRAECDPLTGLANRRGFDAQFAAQCAAQFRTGTAGSGPEPLGALLLIDLDGFKPVNDIHGHAAGDL
CLRRAAARLAAVCRDATLVARIGGDEFAVLLRAPVDPSGLVERGRRIVAALSRPVPYRGHGLRIGASVGIAVGTAAGTAA
GGGPARLFGRADAALYAAKDAGGNTVRIARGGGRSGAGAAQTAVARGPG
>Mature_368_residues
TTARSLALVKSPRDRSVRGRADPSAADEAVAEAAAACALQRAIIRQQAGLLARQERVLADAGMGLWSCRLHDGALDWSGG
VHDLFGVPRGAALHRPRILDLYEPASRRLLETLRHRAIARGGAFALDAEVLAGGRRRWIRIRAAVESEDGRPTRLFGTKQ
DVTEEIGRLAELSRRAECDPLTGLANRRGFDAQFAAQCAAQFRTGTAGSGPEPLGALLLIDLDGFKPVNDIHGHAAGDLC
LRRAAARLAAVCRDATLVARIGGDEFAVLLRAPVDPSGLVERGRRIVAALSRPVPYRGHGLRIGASVGIAVGTAAGTAAG
GGPARLFGRADAALYAAKDAGGNTVRIARGGGRSGAGAAQTAVARGPG

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=39.0374331550802, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081881, Length=249, Percent_Identity=32.5301204819277, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=178, Percent_Identity=33.7078651685393, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=32.5581395348837, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693134, Length=213, Percent_Identity=32.3943661971831, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=29.7297297297297, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788956, Length=193, Percent_Identity=32.6424870466321, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787541, Length=321, Percent_Identity=29.9065420560748, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786584, Length=193, Percent_Identity=29.0155440414508, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013655 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF05231 MASE1; PF08447 PAS_3; PF08448 PAS_4 [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38415; Mature: 38284

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTARSLALVKSPRDRSVRGRADPSAADEAVAEAAAACALQRAIIRQQAGLLARQERVLA
CCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAGMGLWSCRLHDGALDWSGGVHDLFGVPRGAALHRPRILDLYEPASRRLLETLRHRAIA
HCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
RGGAFALDAEVLAGGRRRWIRIRAAVESEDGRPTRLFGTKQDVTEEIGRLAELSRRAECD
CCCCEEECHHHHCCCCEEEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCC
PLTGLANRRGFDAQFAAQCAAQFRTGTAGSGPEPLGALLLIDLDGFKPVNDIHGHAAGDL
CCCCHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCHHHHH
CLRRAAARLAAVCRDATLVARIGGDEFAVLLRAPVDPSGLVERGRRIVAALSRPVPYRGH
HHHHHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
GLRIGASVGIAVGTAAGTAAGGGPARLFGRADAALYAAKDAGGNTVRIARGGGRSGAGAA
CEEECCCCCEEEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCHH
QTAVARGPG
HHHHCCCCC
>Mature Secondary Structure 
TTARSLALVKSPRDRSVRGRADPSAADEAVAEAAAACALQRAIIRQQAGLLARQERVLA
CCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAGMGLWSCRLHDGALDWSGGVHDLFGVPRGAALHRPRILDLYEPASRRLLETLRHRAIA
HCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
RGGAFALDAEVLAGGRRRWIRIRAAVESEDGRPTRLFGTKQDVTEEIGRLAELSRRAECD
CCCCEEECHHHHCCCCEEEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCC
PLTGLANRRGFDAQFAAQCAAQFRTGTAGSGPEPLGALLLIDLDGFKPVNDIHGHAAGDL
CCCCHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCHHHHH
CLRRAAARLAAVCRDATLVARIGGDEFAVLLRAPVDPSGLVERGRRIVAALSRPVPYRGH
HHHHHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
GLRIGASVGIAVGTAAGTAAGGGPARLFGRADAALYAAKDAGGNTVRIARGGGRSGAGAA
CEEECCCCCEEEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCHH
QTAVARGPG
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 6094528; 7984428 [H]