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The map label for this gene is tilS [H]

Identifier: 170750723

GI number: 170750723

Start: 4590178

End: 4591215

Strand: Direct

Name: tilS [H]

Synonym: Mrad2831_4334

Alternate gene names: 170750723

Gene position: 4590178-4591215 (Clockwise)

Preceding gene: 170750722

Following gene: 170750724

Centisome position: 75.52

GC content: 80.64

Gene sequence:

>1038_bases
GTGCTCCTCGCCGTCTCGGGCGGTCCCGACTCGAGCGCCCTGATGCACGCCGCCGCGCGCCTGCGCGACGCGACCGGCGC
GTCCCTGCGGGTCGCCACCGTGGATCACGGCCTGCGGTCGGAATCGGGCGCGGAGGCCGAGGCCGTCGGCCGCGCCGCCC
GGGCGCTGGGCCTGCCCCACGCAATCCTGACCTGGGCGGGCGCGAAGCCCGGCACCGGCCTGCAGGACGCGGCCCGCAGG
GCCCGCTACGCCCTGCTCGCCGCCCATGCCGAGGCGGTCGGCGCCGGTCTCGTGCTGACGGGCCACACGCGGGACGACCA
GGCCGAGACGGTGCTGATCCGGCTCGCGGCCGGCAGCGGCCCCGCGGGGCTCGCCGGCATGCGCGCCGAGCGCGACCTCG
GCCCCGGCCTCGCCCTCGGGCGCCCGTTCCTGCACCTGCCGAAGGCGAGCCTGGTGGCGTGGTGCGCGGCGCGCGGCATC
GCCTACGCGCGCGACCCCGCCAACGGCGATCCGCGCTTCGCCCGCGGCCGCCTGCGGGCGGCCTGGCCGGCCCTGGAGCG
CGAGGGCCTGACCCGGGCGCGCCTCGCCCGGCTCGCCGAGCGGGCCGCCCGCGACGAGGCCGCGCTCCGGAGCGCGGCCG
AGCAGGCCCTCGACGGGGCGCGGCGGTCCGGGCCGGAGACGGGCGCGGAGCTGCGCCTCGACGGGCGCGCCCTGGCGGCC
CTGCCGGACGCCGTGGCCCTGCGCTGCCTCGATCTCGCCCTGGCCCGGGCCGGCGCGGCGCCGCGCCGCCTCGAGCGCCT
CGAGACCCTCGTCCTCGAATCGCTCCTGCCGGCCCTGCGCCGCGGGGAGCCGGTCCGCCGCACCCTGGCGGGGTTCCTCG
TCTCCGCCGACGCGGGCGGGACCGTGAGCCTCGCCCCGGCGCCGCCGCGCCGGCACGGGCCCGCAGGTGCTGCCCTCGCC
GCAGGGTCCCCCGACTTACTTGGCAAGGGGGAGCCCCCCGCCTACATTGGCGAGGTGTGCACGGATGGACCGGAATAG

Upstream 100 bases:

>100_bases
GCGGCGCCTGAACGCCTCGGGCGATCTGAGCGACGCGGGCGACCCGGTCCTCGGCGCCCTCCGCCCCTGGCTCGACCCGG
CCGCGCGCGGCGGGCGGGGC

Downstream 100 bases:

>100_bases
CCGCGTGGCAGGTCCGTCCTGGGGGTCGCACACGCCAGGGATTGATCGATGAACCCGAATTTTCGCAACTTTGCCTTGTG
GGTCGTCATCTTCCTGCTGG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MLLAVSGGPDSSALMHAAARLRDATGASLRVATVDHGLRSESGAEAEAVGRAARALGLPHAILTWAGAKPGTGLQDAARR
ARYALLAAHAEAVGAGLVLTGHTRDDQAETVLIRLAAGSGPAGLAGMRAERDLGPGLALGRPFLHLPKASLVAWCAARGI
AYARDPANGDPRFARGRLRAAWPALEREGLTRARLARLAERAARDEAALRSAAEQALDGARRSGPETGAELRLDGRALAA
LPDAVALRCLDLALARAGAAPRRLERLETLVLESLLPALRRGEPVRRTLAGFLVSADAGGTVSLAPAPPRRHGPAGAALA
AGSPDLLGKGEPPAYIGEVCTDGPE

Sequences:

>Translated_345_residues
MLLAVSGGPDSSALMHAAARLRDATGASLRVATVDHGLRSESGAEAEAVGRAARALGLPHAILTWAGAKPGTGLQDAARR
ARYALLAAHAEAVGAGLVLTGHTRDDQAETVLIRLAAGSGPAGLAGMRAERDLGPGLALGRPFLHLPKASLVAWCAARGI
AYARDPANGDPRFARGRLRAAWPALEREGLTRARLARLAERAARDEAALRSAAEQALDGARRSGPETGAELRLDGRALAA
LPDAVALRCLDLALARAGAAPRRLERLETLVLESLLPALRRGEPVRRTLAGFLVSADAGGTVSLAPAPPRRHGPAGAALA
AGSPDLLGKGEPPAYIGEVCTDGPE
>Mature_345_residues
MLLAVSGGPDSSALMHAAARLRDATGASLRVATVDHGLRSESGAEAEAVGRAARALGLPHAILTWAGAKPGTGLQDAARR
ARYALLAAHAEAVGAGLVLTGHTRDDQAETVLIRLAAGSGPAGLAGMRAERDLGPGLALGRPFLHLPKASLVAWCAARGI
AYARDPANGDPRFARGRLRAAWPALEREGLTRARLARLAERAARDEAALRSAAEQALDGARRSGPETGAELRLDGRALAA
LPDAVALRCLDLALARAGAAPRRLERLETLVLESLLPALRRGEPVRRTLAGFLVSADAGGTVSLAPAPPRRHGPAGAALA
AGSPDLLGKGEPPAYIGEVCTDGPE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=209, Percent_Identity=34.4497607655502, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35358; Mature: 35358

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAVSGGPDSSALMHAAARLRDATGASLRVATVDHGLRSESGAEAEAVGRAARALGLPH
CEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCH
AILTWAGAKPGTGLQDAARRARYALLAAHAEAVGAGLVLTGHTRDDQAETVLIRLAAGSG
HHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCC
PAGLAGMRAERDLGPGLALGRPFLHLPKASLVAWCAARGIAYARDPANGDPRFARGRLRA
CCCCCCCCHHHCCCCCHHHCCCHHHCCHHHHHHHHHHCCCEEECCCCCCCCHHHHCCHHH
AWPALEREGLTRARLARLAERAARDEAALRSAAEQALDGARRSGPETGAELRLDGRALAA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHH
LPDAVALRCLDLALARAGAAPRRLERLETLVLESLLPALRRGEPVRRTLAGFLVSADAGG
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCC
TVSLAPAPPRRHGPAGAALAAGSPDLLGKGEPPAYIGEVCTDGPE
EEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MLLAVSGGPDSSALMHAAARLRDATGASLRVATVDHGLRSESGAEAEAVGRAARALGLPH
CEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCH
AILTWAGAKPGTGLQDAARRARYALLAAHAEAVGAGLVLTGHTRDDQAETVLIRLAAGSG
HHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCC
PAGLAGMRAERDLGPGLALGRPFLHLPKASLVAWCAARGIAYARDPANGDPRFARGRLRA
CCCCCCCCHHHCCCCCHHHCCCHHHCCHHHHHHHHHHCCCEEECCCCCCCCHHHHCCHHH
AWPALEREGLTRARLARLAERAARDEAALRSAAEQALDGARRSGPETGAELRLDGRALAA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHH
LPDAVALRCLDLALARAGAAPRRLERLETLVLESLLPALRRGEPVRRTLAGFLVSADAGG
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCC
TVSLAPAPPRRHGPAGAALAAGSPDLLGKGEPPAYIGEVCTDGPE
EEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA