Click here to switch to the map view.

The map label for this gene is glcD [H]

Identifier: 170750714

GI number: 170750714

Start: 4581006

End: 4582439

Strand: Reverse

Name: glcD [H]

Synonym: Mrad2831_4325

Alternate gene names: 170750714

Gene position: 4582439-4581006 (Counterclockwise)

Preceding gene: 170750716

Following gene: 170750709

Centisome position: 75.4

GC content: 70.64

Gene sequence:

>1434_bases
ATGACCATCCGCTTCCCCGATCCCGATCCCGCGATCCTCGCCCGCCGCGAGGCGATCCTGGCCGGCCTCGCGCCCCTGGT
GGCGCCGGAGGCGCTGGTGGTGAGCGAGGACGAGCGCCGGGCCTTCGAGACCGACGGGCTGACCGCGTACCGGCAGATGC
CGCTGGCCGTGGTCCTGCCCTCCACCACCGCGGAGGTCTCCGCCGTGATGGCCTATTGCCACGCCAACGGCGTGCGGGTC
GTGCCCCGGGGCGCCGGCACGTCGCTCGCGGGCGGCGCCATCGCGCAGGCCGACGCGATCATCCTGGGCGTCGGCAAGAT
GACCAAGGTCCTCGACATGGACTTCGCCAACCGCACCGCCCGGGTGCAGAGCGGCATCACCAACCTCGCCATCTCCGGGG
CGGTCAGCCACGAGGGCTTCTTCTACGCCCCCGACCCGTCGAGCCAGCTCGCCTGCACGATCGCCGGCAACATCGCGATG
AATTCCGGCGGCGCGCACTGTCTGAAATACGGCGTGACCACCAACAACCTGCTCGGCGTCACCCTGGTGCTCAACGACGG
CACCGTCGTGGAGATCGGCGGCCAGCACCTCGACAGCGGCGGCTACGACCTGCTCGGCCTCGTCTGCGGCTCGGAGGGGC
AGCTCGGCATCGTCACCGAGGCGACCGTGCGGATCCTGCGCGCCGCCGAGGGCGCGCGCCCGGTGCTGGTCGGCTTCTCG
AGGGTCGAGGACGCGGGCGACTGCGTCGCGGCGATTATCGCGGCCGGGATCATCCCGGTGGCGATCGAGTACATGGACCG
CGAGGCGATCCTCATCACCGAGGACTTCGCCCAGGCCGGCTATCCCCGCGACGCCGAGGCGATGCTGATCATCGAGGTCG
AGGGCTCGGACGCCGAGTGCGACGCCATGCTGGCGCGGATCGAGACGATCGCCCGGGATTTCCGCCCGACCAGCGTGCGC
GTCTCGCGCTCGGAGGCGGAGTCGGCCGCGATCTGGAAGGGGCGCAAGTCGGCCTTCGGGGCCACCGGCCGGATCTCCGA
CTACATCTGCATGGACGGCACGATCCCCACCGGCCAGCTCGGGCCGGTGCTGGAGCGGATCGGGGCGATCTGCGCCGAGA
AGGGCCTGCGGGTCGCCAACGTGTTCCACGCGGGCGACGGCAACCTGCACCCGCTGATCCTGTTCGACATCAACCGGCCC
GGCGAGCTGCAGAAGGCCGAGGCCGCGGGCGACGAGATCCTGAAGCTCTGCGTCGAGGTCGGCGGCTGCCTGACCGGTGA
GCACGGCGTCGGGATCGAGAAGCGCGAGCTGATGCGCTTCCAGTACGATCAGGTCGATCTCGAGCAGCAGATGCGGGTCA
AGGCGGTGTTCGATCCGGACTGGATGCTGAACCCCGCCAAGGTGTTCCCGCTGGACGGGCGCCTCGCGGCCTGA

Upstream 100 bases:

>100_bases
GGACGACGCAGCGCCGCACTCTGGTTGAAGGCGCGGCCGCCGAGGTCCATGCTGCCCGCACACCGTCCCGCGCCGCGGGC
CCCAGGAGGGCACGAGCCCT

Downstream 100 bases:

>100_bases
ACCGGCCCGCGCGGCGTGGATGAGCCGGACCGCGCGGCGGGGTCCGGCCGAGGTGCAGGCCAGGACGCACGGCTAGGACG
TACGGCCGAGGCGTCCGGAG

Product: FAD linked oxidase domain-containing protein

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTIRFPDPDPAILARREAILAGLAPLVAPEALVVSEDERRAFETDGLTAYRQMPLAVVLPSTTAEVSAVMAYCHANGVRV
VPRGAGTSLAGGAIAQADAIILGVGKMTKVLDMDFANRTARVQSGITNLAISGAVSHEGFFYAPDPSSQLACTIAGNIAM
NSGGAHCLKYGVTTNNLLGVTLVLNDGTVVEIGGQHLDSGGYDLLGLVCGSEGQLGIVTEATVRILRAAEGARPVLVGFS
RVEDAGDCVAAIIAAGIIPVAIEYMDREAILITEDFAQAGYPRDAEAMLIIEVEGSDAECDAMLARIETIARDFRPTSVR
VSRSEAESAAIWKGRKSAFGATGRISDYICMDGTIPTGQLGPVLERIGAICAEKGLRVANVFHAGDGNLHPLILFDINRP
GELQKAEAAGDEILKLCVEVGGCLTGEHGVGIEKRELMRFQYDQVDLEQQMRVKAVFDPDWMLNPAKVFPLDGRLAA

Sequences:

>Translated_477_residues
MTIRFPDPDPAILARREAILAGLAPLVAPEALVVSEDERRAFETDGLTAYRQMPLAVVLPSTTAEVSAVMAYCHANGVRV
VPRGAGTSLAGGAIAQADAIILGVGKMTKVLDMDFANRTARVQSGITNLAISGAVSHEGFFYAPDPSSQLACTIAGNIAM
NSGGAHCLKYGVTTNNLLGVTLVLNDGTVVEIGGQHLDSGGYDLLGLVCGSEGQLGIVTEATVRILRAAEGARPVLVGFS
RVEDAGDCVAAIIAAGIIPVAIEYMDREAILITEDFAQAGYPRDAEAMLIIEVEGSDAECDAMLARIETIARDFRPTSVR
VSRSEAESAAIWKGRKSAFGATGRISDYICMDGTIPTGQLGPVLERIGAICAEKGLRVANVFHAGDGNLHPLILFDINRP
GELQKAEAAGDEILKLCVEVGGCLTGEHGVGIEKRELMRFQYDQVDLEQQMRVKAVFDPDWMLNPAKVFPLDGRLAA
>Mature_476_residues
TIRFPDPDPAILARREAILAGLAPLVAPEALVVSEDERRAFETDGLTAYRQMPLAVVLPSTTAEVSAVMAYCHANGVRVV
PRGAGTSLAGGAIAQADAIILGVGKMTKVLDMDFANRTARVQSGITNLAISGAVSHEGFFYAPDPSSQLACTIAGNIAMN
SGGAHCLKYGVTTNNLLGVTLVLNDGTVVEIGGQHLDSGGYDLLGLVCGSEGQLGIVTEATVRILRAAEGARPVLVGFSR
VEDAGDCVAAIIAAGIIPVAIEYMDREAILITEDFAQAGYPRDAEAMLIIEVEGSDAECDAMLARIETIARDFRPTSVRV
SRSEAESAAIWKGRKSAFGATGRISDYICMDGTIPTGQLGPVLERIGAICAEKGLRVANVFHAGDGNLHPLILFDINRPG
ELQKAEAAGDEILKLCVEVGGCLTGEHGVGIEKRELMRFQYDQVDLEQQMRVKAVFDPDWMLNPAKVFPLDGRLAA

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=428, Percent_Identity=27.5700934579439, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI37595754, Length=451, Percent_Identity=26.3858093126386, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI119964728, Length=453, Percent_Identity=28.476821192053, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI4501993, Length=471, Percent_Identity=22.5053078556263, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1789351, Length=462, Percent_Identity=48.9177489177489, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1787977, Length=518, Percent_Identity=24.5173745173745, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI48994907, Length=512, Percent_Identity=22.265625, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=476, Percent_Identity=26.2605042016807, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71992381, Length=473, Percent_Identity=25.7928118393235, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17534361, Length=476, Percent_Identity=27.3109243697479, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71992392, Length=362, Percent_Identity=26.5193370165746, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17556096, Length=289, Percent_Identity=22.1453287197232, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320027, Length=434, Percent_Identity=29.0322580645161, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320023, Length=466, Percent_Identity=25.9656652360515, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320764, Length=481, Percent_Identity=24.5322245322245, Blast_Score=149, Evalue=7e-37,
Organism=Drosophila melanogaster, GI18921117, Length=423, Percent_Identity=26.7139479905437, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24639277, Length=423, Percent_Identity=26.7139479905437, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24639275, Length=423, Percent_Identity=26.7139479905437, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24653753, Length=476, Percent_Identity=22.4789915966387, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50403; Mature: 50272

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRFPDPDPAILARREAILAGLAPLVAPEALVVSEDERRAFETDGLTAYRQMPLAVVLP
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCHHHHHCCEEEEEC
STTAEVSAVMAYCHANGVRVVPRGAGTSLAGGAIAQADAIILGVGKMTKVLDMDFANRTA
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHCCCEEEEECCHHHHHHHCCCCCCHH
RVQSGITNLAISGAVSHEGFFYAPDPSSQLACTIAGNIAMNSGGAHCLKYGVTTNNLLGV
HHHCCCCCEEEECEECCCCEEECCCCCCCEEEEEECCEEECCCCCEEEEECCCCCCEEEE
TLVLNDGTVVEIGGQHLDSGGYDLLGLVCGSEGQLGIVTEATVRILRAAEGARPVLVGFS
EEEECCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCEEEEEHH
RVEDAGDCVAAIIAAGIIPVAIEYMDREAILITEDFAQAGYPRDAEAMLIIEVEGSDAEC
HCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEEEEEEECCCCCHH
DAMLARIETIARDFRPTSVRVSRSEAESAAIWKGRKSAFGATGRISDYICMDGTIPTGQL
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCH
GPVLERIGAICAEKGLRVANVFHAGDGNLHPLILFDINRPGELQKAEAAGDEILKLCVEV
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
GGCLTGEHGVGIEKRELMRFQYDQVDLEQQMRVKAVFDPDWMLNPAKVFPLDGRLAA
CCCEECCCCCCCCHHHHHHHHCCCCCHHHHCEEEEEECCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TIRFPDPDPAILARREAILAGLAPLVAPEALVVSEDERRAFETDGLTAYRQMPLAVVLP
EEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCHHHHHCCEEEEEC
STTAEVSAVMAYCHANGVRVVPRGAGTSLAGGAIAQADAIILGVGKMTKVLDMDFANRTA
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHCCCEEEEECCHHHHHHHCCCCCCHH
RVQSGITNLAISGAVSHEGFFYAPDPSSQLACTIAGNIAMNSGGAHCLKYGVTTNNLLGV
HHHCCCCCEEEECEECCCCEEECCCCCCCEEEEEECCEEECCCCCEEEEECCCCCCEEEE
TLVLNDGTVVEIGGQHLDSGGYDLLGLVCGSEGQLGIVTEATVRILRAAEGARPVLVGFS
EEEECCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCEEEEEHH
RVEDAGDCVAAIIAAGIIPVAIEYMDREAILITEDFAQAGYPRDAEAMLIIEVEGSDAEC
HCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEEEEEEECCCCCHH
DAMLARIETIARDFRPTSVRVSRSEAESAAIWKGRKSAFGATGRISDYICMDGTIPTGQL
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCH
GPVLERIGAICAEKGLRVANVFHAGDGNLHPLILFDINRPGELQKAEAAGDEILKLCVEV
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
GGCLTGEHGVGIEKRELMRFQYDQVDLEQQMRVKAVFDPDWMLNPAKVFPLDGRLAA
CCCEECCCCCCCCHHHHHHHHCCCCCHHHHCEEEEEECCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]