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The map label for this gene is oprM [H]

Identifier: 170750705

GI number: 170750705

Start: 4566064

End: 4567599

Strand: Reverse

Name: oprM [H]

Synonym: Mrad2831_4316

Alternate gene names: 170750705

Gene position: 4567599-4566064 (Counterclockwise)

Preceding gene: 170750706

Following gene: 170750704

Centisome position: 75.15

GC content: 72.46

Gene sequence:

>1536_bases
ATGGGGTTCGGGACGGGCGGCGCGCTTGCGACCGATCGTCGGCTTCACGCCCTCGCCGCCCTGCTCCTCGCCGCCCCCCT
CATGACCGGCTGCGTGGTCGGGCCCGACTACACCCGGCCCTCCGTGGAGACACCGCTCGCCTACAAGCAGGGCGGCGTCC
GGGAGGACAGCCCGGCCTACGTCGCGGCGCGCAGGAAGGGCTGGCGCGAGGCCCGGCCGAGCGACGCCGTCGAGCGCGGC
GACTGGTGGCGGGTGTTCAAGGATCCGGCTCTCGACCGCCTGATCCGCCTCGTCGACGTCGACAACCAGTCCCTCCGGCA
GGCGGTGGCGAATTACCGGCAGGCCCGGGCGCTCGTCGCCTCCGCGCAGGCCGCCCTGTACCCGACCGTGATCGGCGCGC
CGAGCATCACCCGGACCGGCAGCGGCACCGCCACGCGCACCAACGTGTCGCTGCTGGGCCAGGCAAGCTGGGAGCTCGAC
CTGTTCGGCGGCACCCGCCGTACCATCGAGAGCGAGGCGGCCCTGGCGCAGTCCGACGCCGCCACGATCGCGCTGACCCG
GCTGAGTATCCAGGCCGAGATCGCGGCCGATTACCTGACGGTGCGCTACGCCGACTCCCTCCAGAAGGTGCTCGACGAGA
ATGTCGAGAACTTCAAGAGAACCCTGGCGATCACCGAGAACCAGTACGCCGCCGGCGTGGCGGCGCGCTCCGACGTGATC
ACCGCGCAGACCCAGGTGCAGACCATCGAGGCCCAGGCCATCGCGGTGCGGCTGACCCGGGTGCAGTACGTCAACGCCAT
CGCCACGCTGATCGGGCGCCCGCCCTCGGAGGTGTCGATCCCGGTGGCCGGCATCCCGCGCAACCCGCCGGGCGTGCCGG
TGGGCATCCCCGGCGACCTGCTGGAGCGGCGCCCCGACGTGGCCCAGGCCGAGCGCCTCGTCCAGGCGCAGAGCGAGCGC
ATCGGCGTCGCGGTGGCGGCCTTCTTCCCCACCGTGACGATCTCGGCCCAGGGCGGCATCTCGGGAGCGACCCGCAATGG
CCTGCTCTCGGCGGCCAACCAAGTCTGGTCGGTGGCGGCCTCCGGCAGCGAGGTGCTGTTCGACGGCGGCGCCCGCACGG
CGGCCCTGCGCTCGGCCGAGGCCGGCTACGACTCGGCGGTGGCCAATTACCGGCAGGTGGTGCTGGCCGCCTTCGCGGAG
GTCGAGAACCAGATGGCGGCCGTGCGTATCCTCGGCCAGCAGCAGCGGGCCCAGGACACCGCGGTGGACCTGTCGCGCAA
GGCCGTGGAGATCACGCTCAACGAGTACCGGGCCGGTACCCAGAACTTCACCACGGTGGTGACCGCCCAGAACCTGCTCG
TGAACAACGAGGTCAACGCCCTCCAAGTGCGGCTGAGCCGCTTCACCGCGGCGGTCACGCTGATCCGGGCACTCGGCGGC
GGCTGGGACGTGCGCGCCCTGCCGAGCGATTCCGAGCTGAAGGGTCCGAGCCTGCCGATCGACCGCGGCGGCCAGGCCGT
CCGCCCCGACGAGTAG

Upstream 100 bases:

>100_bases
TCCCGAGGTGCTGCGTCGCGGCCTCGAAGGAGGCCTGCGGGGATCGCGCGGCCTGCCGGGAGGCTCTTTCGAGGCCTCCG
CCACGCTTCGGCACCTCCGG

Downstream 100 bases:

>100_bases
CGGCCGGCGGGCCGCGTGATGGTGCGGCCCGGAGCGTCCAACCCGCACCCTCGTCCTGAGCGGTGACCGTGGTCATCGTG
CAGCGGCCGGACGCTCGCGA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MGFGTGGALATDRRLHALAALLLAAPLMTGCVVGPDYTRPSVETPLAYKQGGVREDSPAYVAARRKGWREARPSDAVERG
DWWRVFKDPALDRLIRLVDVDNQSLRQAVANYRQARALVASAQAALYPTVIGAPSITRTGSGTATRTNVSLLGQASWELD
LFGGTRRTIESEAALAQSDAATIALTRLSIQAEIAADYLTVRYADSLQKVLDENVENFKRTLAITENQYAAGVAARSDVI
TAQTQVQTIEAQAIAVRLTRVQYVNAIATLIGRPPSEVSIPVAGIPRNPPGVPVGIPGDLLERRPDVAQAERLVQAQSER
IGVAVAAFFPTVTISAQGGISGATRNGLLSAANQVWSVAASGSEVLFDGGARTAALRSAEAGYDSAVANYRQVVLAAFAE
VENQMAAVRILGQQQRAQDTAVDLSRKAVEITLNEYRAGTQNFTTVVTAQNLLVNNEVNALQVRLSRFTAAVTLIRALGG
GWDVRALPSDSELKGPSLPIDRGGQAVRPDE

Sequences:

>Translated_511_residues
MGFGTGGALATDRRLHALAALLLAAPLMTGCVVGPDYTRPSVETPLAYKQGGVREDSPAYVAARRKGWREARPSDAVERG
DWWRVFKDPALDRLIRLVDVDNQSLRQAVANYRQARALVASAQAALYPTVIGAPSITRTGSGTATRTNVSLLGQASWELD
LFGGTRRTIESEAALAQSDAATIALTRLSIQAEIAADYLTVRYADSLQKVLDENVENFKRTLAITENQYAAGVAARSDVI
TAQTQVQTIEAQAIAVRLTRVQYVNAIATLIGRPPSEVSIPVAGIPRNPPGVPVGIPGDLLERRPDVAQAERLVQAQSER
IGVAVAAFFPTVTISAQGGISGATRNGLLSAANQVWSVAASGSEVLFDGGARTAALRSAEAGYDSAVANYRQVVLAAFAE
VENQMAAVRILGQQQRAQDTAVDLSRKAVEITLNEYRAGTQNFTTVVTAQNLLVNNEVNALQVRLSRFTAAVTLIRALGG
GWDVRALPSDSELKGPSLPIDRGGQAVRPDE
>Mature_510_residues
GFGTGGALATDRRLHALAALLLAAPLMTGCVVGPDYTRPSVETPLAYKQGGVREDSPAYVAARRKGWREARPSDAVERGD
WWRVFKDPALDRLIRLVDVDNQSLRQAVANYRQARALVASAQAALYPTVIGAPSITRTGSGTATRTNVSLLGQASWELDL
FGGTRRTIESEAALAQSDAATIALTRLSIQAEIAADYLTVRYADSLQKVLDENVENFKRTLAITENQYAAGVAARSDVIT
AQTQVQTIEAQAIAVRLTRVQYVNAIATLIGRPPSEVSIPVAGIPRNPPGVPVGIPGDLLERRPDVAQAERLVQAQSERI
GVAVAAFFPTVTISAQGGISGATRNGLLSAANQVWSVAASGSEVLFDGGARTAALRSAEAGYDSAVANYRQVVLAAFAEV
ENQMAAVRILGQQQRAQDTAVDLSRKAVEITLNEYRAGTQNFTTVVTAQNLLVNNEVNALQVRLSRFTAAVTLIRALGGG
WDVRALPSDSELKGPSLPIDRGGQAVRPDE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=474, Percent_Identity=27.8481012658228, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI2367348, Length=487, Percent_Identity=22.17659137577, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54345; Mature: 54214

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFGTGGALATDRRLHALAALLLAAPLMTGCVVGPDYTRPSVETPLAYKQGGVREDSPAY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHCCCCCCCCCHH
VAARRKGWREARPSDAVERGDWWRVFKDPALDRLIRLVDVDNQSLRQAVANYRQARALVA
HHHHHCCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SAQAALYPTVIGAPSITRTGSGTATRTNVSLLGQASWELDLFGGTRRTIESEAALAQSDA
HHHHHHHHHEECCCCCEECCCCCCEECCEEEEECCCEEEEECCCCCHHHHHHHHHHHCCC
ATIALTRLSIQAEIAADYLTVRYADSLQKVLDENVENFKRTLAITENQYAAGVAARSDVI
CEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHCHHHCCCEE
TAQTQVQTIEAQAIAVRLTRVQYVNAIATLIGRPPSEVSIPVAGIPRNPPGVPVGIPGDL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCHHH
LERRPDVAQAERLVQAQSERIGVAVAAFFPTVTISAQGGISGATRNGLLSAANQVWSVAA
HHCCCCHHHHHHHHHHHHHHHCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHC
SGSEVLFDGGARTAALRSAEAGYDSAVANYRQVVLAAFAEVENQMAAVRILGQQQRAQDT
CCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AVDLSRKAVEITLNEYRAGTQNFTTVVTAQNLLVNNEVNALQVRLSRFTAAVTLIRALGG
HHHHCCCEEEEEEHHHCCCCCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHCC
GWDVRALPSDSELKGPSLPIDRGGQAVRPDE
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GFGTGGALATDRRLHALAALLLAAPLMTGCVVGPDYTRPSVETPLAYKQGGVREDSPAY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHCCCCCCCCCHH
VAARRKGWREARPSDAVERGDWWRVFKDPALDRLIRLVDVDNQSLRQAVANYRQARALVA
HHHHHCCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SAQAALYPTVIGAPSITRTGSGTATRTNVSLLGQASWELDLFGGTRRTIESEAALAQSDA
HHHHHHHHHEECCCCCEECCCCCCEECCEEEEECCCEEEEECCCCCHHHHHHHHHHHCCC
ATIALTRLSIQAEIAADYLTVRYADSLQKVLDENVENFKRTLAITENQYAAGVAARSDVI
CEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHCHHHCCCEE
TAQTQVQTIEAQAIAVRLTRVQYVNAIATLIGRPPSEVSIPVAGIPRNPPGVPVGIPGDL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCHHH
LERRPDVAQAERLVQAQSERIGVAVAAFFPTVTISAQGGISGATRNGLLSAANQVWSVAA
HHCCCCHHHHHHHHHHHHHHHCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHC
SGSEVLFDGGARTAALRSAEAGYDSAVANYRQVVLAAFAEVENQMAAVRILGQQQRAQDT
CCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AVDLSRKAVEITLNEYRAGTQNFTTVVTAQNLLVNNEVNALQVRLSRFTAAVTLIRALGG
HHHHCCCEEEEEEHHHCCCCCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHCC
GWDVRALPSDSELKGPSLPIDRGGQAVRPDE
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]