Click here to switch to the map view.

The map label for this gene is arsB [H]

Identifier: 170750611

GI number: 170750611

Start: 4470479

End: 4471747

Strand: Reverse

Name: arsB [H]

Synonym: Mrad2831_4221

Alternate gene names: 170750611

Gene position: 4471747-4470479 (Counterclockwise)

Preceding gene: 170750612

Following gene: 170750610

Centisome position: 73.57

GC content: 72.58

Gene sequence:

>1269_bases
ATGGGCGCGCTGACGCTGACCCCCAACATCGCGACCTGGACGATCGCCGCCCTCGCGACCCTCGGCGTCATCCTGCGGCC
GTTCTCGTGGCCGGAGGCGATCTGGGCCGTGCTCGGCGCGGGCGCCCTCGTGGCCCTCGGGCTGCTCGCCCCGGCCACCG
CCTGGGACGGCATCCTGAAGGGGACCGACGTCTACCTGTTCCTCGTCGGCATGATGCTGATGTCGGAGGTCGCCCGGAAG
GAGGGCCTGTTCGACTGGCTCGCGTCGATCGCCGTGCGCGCCGCCAGGGGCTCGGCGACCCGGCTGTTTACCCTCGTGTA
CCTCGTCGGCACCGTCGTCACGGTGTTCCTGTCGAACGACGCCTGCGCGGTGGTGCTGACGCCGGCCGTCTACGCGGCCA
CCAAGGCCGCCAAGGTGGAGAACCCCCTCCCCTACCTGTTCATCTGCGCCTTCATCGCCAACGCGGCGAGCTTCGTGCTG
CCGATCTCGAACCCGGCCAACCTCGTGGTCTTCGCCGAGCACATGCCGCCGCTGGTGCGCTGGCTCGGCCTGTTCGCCCT
GCCCTCGCTGCTGGCCATCGCGACGACCTACGCGGTGCTGCGGCTGACCCAGGCGAAGACCCTGCGCGGGCAGCAGGTCG
CCACCGACGTCGAGGCCGTGGCGCTGTCCCGCACCGGCCTCGTGGCGGGGATCGGCATCGTGGCGACCGGCGGCGTGCTG
ATCGCCGCCTCGGCGCTCGGCCTCGACCTCGGCCTGCCGACCTTCGTGGCCGGCCTCGCCACCACGGTCGTCGTGCTGCT
GCTCAAGCGCGGCGGCCTCGTCGAGGTGGCCAAGGACGTGTCGTGGAGCGTGCTGCCGCTGGTGGCCGGGCTGTTCGTCC
TGGTGGAGGCGCTGGAGAAGACCGGCGTGCTCGCCATGCTGGCGGACCTGCTCAAGGGCTACGCCCACGCCGCCCCGGAG
GCCACCGCCTGGGGGGCGGGCGGCCTGGTCGCGGTGCTGTGCAACCTCGTCAACAACCTGCCGGCCGGGCTGATCGCGGG
CGCCGCCGTGCAGACCGCCCACGTGCCCGAGAAGGTGGCGGGCGCGATCCTGATCGGCGTCGACCTCGGGCCGAACCTGT
CGGTGACGGGCTCGCTCGCCACGATCCTCTGGCTCACCGCCATCCGCCGCGAGGGCCAGGATGTCGGCGCCTGGACCTTC
CTCAAGCTCGGCCTGCTGGTGATGCCGCCGGCCCTCGCGCTGGCGCTCGCCGGCCTGCTCCTCGTCTGA

Upstream 100 bases:

>100_bases
CTCGGGCCGAACCGCAACGGCCGGGACTGGTAGCCGGTCCCGACCAAATCGCGGCACACCGCACCGCACGATCCACCCCA
CCAGAGGACCACCGCAACCG

Downstream 100 bases:

>100_bases
CCCTCCCTCCCCGCCAACCCGAAGGTCCGCGCGCGTGACGGCTGCCCTCCTCTATCCCTTCATCCTGGTGGCGGGCGCGC
TGCAGGCGATCGGCAACGCC

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MGALTLTPNIATWTIAALATLGVILRPFSWPEAIWAVLGAGALVALGLLAPATAWDGILKGTDVYLFLVGMMLMSEVARK
EGLFDWLASIAVRAARGSATRLFTLVYLVGTVVTVFLSNDACAVVLTPAVYAATKAAKVENPLPYLFICAFIANAASFVL
PISNPANLVVFAEHMPPLVRWLGLFALPSLLAIATTYAVLRLTQAKTLRGQQVATDVEAVALSRTGLVAGIGIVATGGVL
IAASALGLDLGLPTFVAGLATTVVVLLLKRGGLVEVAKDVSWSVLPLVAGLFVLVEALEKTGVLAMLADLLKGYAHAAPE
ATAWGAGGLVAVLCNLVNNLPAGLIAGAAVQTAHVPEKVAGAILIGVDLGPNLSVTGSLATILWLTAIRREGQDVGAWTF
LKLGLLVMPPALALALAGLLLV

Sequences:

>Translated_422_residues
MGALTLTPNIATWTIAALATLGVILRPFSWPEAIWAVLGAGALVALGLLAPATAWDGILKGTDVYLFLVGMMLMSEVARK
EGLFDWLASIAVRAARGSATRLFTLVYLVGTVVTVFLSNDACAVVLTPAVYAATKAAKVENPLPYLFICAFIANAASFVL
PISNPANLVVFAEHMPPLVRWLGLFALPSLLAIATTYAVLRLTQAKTLRGQQVATDVEAVALSRTGLVAGIGIVATGGVL
IAASALGLDLGLPTFVAGLATTVVVLLLKRGGLVEVAKDVSWSVLPLVAGLFVLVEALEKTGVLAMLADLLKGYAHAAPE
ATAWGAGGLVAVLCNLVNNLPAGLIAGAAVQTAHVPEKVAGAILIGVDLGPNLSVTGSLATILWLTAIRREGQDVGAWTF
LKLGLLVMPPALALALAGLLLV
>Mature_421_residues
GALTLTPNIATWTIAALATLGVILRPFSWPEAIWAVLGAGALVALGLLAPATAWDGILKGTDVYLFLVGMMLMSEVARKE
GLFDWLASIAVRAARGSATRLFTLVYLVGTVVTVFLSNDACAVVLTPAVYAATKAAKVENPLPYLFICAFIANAASFVLP
ISNPANLVVFAEHMPPLVRWLGLFALPSLLAIATTYAVLRLTQAKTLRGQQVATDVEAVALSRTGLVAGIGIVATGGVLI
AASALGLDLGLPTFVAGLATTVVVLLLKRGGLVEVAKDVSWSVLPLVAGLFVLVEALEKTGVLAMLADLLKGYAHAAPEA
TAWGAGGLVAVLCNLVNNLPAGLIAGAAVQTAHVPEKVAGAILIGVDLGPNLSVTGSLATILWLTAIRREGQDVGAWTFL
KLGLLVMPPALALALAGLLLV

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=403, Percent_Identity=25.3101736972705, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 43380; Mature: 43249

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGALTLTPNIATWTIAALATLGVILRPFSWPEAIWAVLGAGALVALGLLAPATAWDGILK
CCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTDVYLFLVGMMLMSEVARKEGLFDWLASIAVRAARGSATRLFTLVYLVGTVVTVFLSND
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
ACAVVLTPAVYAATKAAKVENPLPYLFICAFIANAASFVLPISNPANLVVFAEHMPPLVR
CEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHEEEECCCCCCEEEEHHCCCHHHH
WLGLFALPSLLAIATTYAVLRLTQAKTLRGQQVATDVEAVALSRTGLVAGIGIVATGGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHH
IAASALGLDLGLPTFVAGLATTVVVLLLKRGGLVEVAKDVSWSVLPLVAGLFVLVEALEK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGVLAMLADLLKGYAHAAPEATAWGAGGLVAVLCNLVNNLPAGLIAGAAVQTAHVPEKVA
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
GAILIGVDLGPNLSVTGSLATILWLTAIRREGQDVGAWTFLKLGLLVMPPALALALAGLL
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LV
HC
>Mature Secondary Structure 
GALTLTPNIATWTIAALATLGVILRPFSWPEAIWAVLGAGALVALGLLAPATAWDGILK
CCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTDVYLFLVGMMLMSEVARKEGLFDWLASIAVRAARGSATRLFTLVYLVGTVVTVFLSND
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
ACAVVLTPAVYAATKAAKVENPLPYLFICAFIANAASFVLPISNPANLVVFAEHMPPLVR
CEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHEEEECCCCCCEEEEHHCCCHHHH
WLGLFALPSLLAIATTYAVLRLTQAKTLRGQQVATDVEAVALSRTGLVAGIGIVATGGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHH
IAASALGLDLGLPTFVAGLATTVVVLLLKRGGLVEVAKDVSWSVLPLVAGLFVLVEALEK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGVLAMLADLLKGYAHAAPEATAWGAGGLVAVLCNLVNNLPAGLIAGAAVQTAHVPEKVA
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
GAILIGVDLGPNLSVTGSLATILWLTAIRREGQDVGAWTFLKLGLLVMPPALALALAGLL
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LV
HC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA