Click here to switch to the map view.

The map label for this gene is tilS/hprT [H]

Identifier: 170750393

GI number: 170750393

Start: 4247777

End: 4248343

Strand: Direct

Name: tilS/hprT [H]

Synonym: Mrad2831_3996

Alternate gene names: 170750393

Gene position: 4247777-4248343 (Clockwise)

Preceding gene: 170750392

Following gene: 170750394

Centisome position: 69.89

GC content: 70.02

Gene sequence:

>567_bases
ATGAGCATCCCTGAACGGCGCGTCCGGACCCTGTTCGACGAGGCGGCCATCGCCAAGCGCAACGACGAGCTCGCCCGGGA
GATCCTCGCCGCGCGGCCCGAGAACCTCCTCGTCGTGGCAGTGCTCAAGGGCAGCTTCATGTTCGCGGCCGACCTGCTCC
GGTCGCTGCACCGGGTCGGGCTCGCCCCGCAGGTCGAGTTCGTGCACCTGTCGAGCTACCGCACCGGGACCGTCTCCAGC
GGGCAGGTCGAGATCCTGCGCGACGTGCAGAGCGAGGTGCGCGGGCGGGACGTGCTGCTCGTGGACGACATCCTGGAATC
GGGCCGGACCGTCGTCTTCGCCAAGGACCTGCTGATGGCCCGCGGCGCCCGGCGGGTCCTCACCGCGGTGCTGCTGGAGA
AGCCCGGCAAGCGCGCCGTGACGATCGACGCGGATTTCGTCGGCTTCACCTGTCCCGACGTGTTCGTCGTCGGCTACGGG
ATGGACGCCGCCCACGCCTTCCGCCAGCTGCCCTTCGTGGGCGTGGTCGATTACGGCGACGGCGACCCGGACCTGTTCGA
CGCTTGA

Upstream 100 bases:

>100_bases
TTGGCGGATCCGGAGTTCCCCGCGCGCGCCCGGGACGCTAAGACCGTCTTCGAGCGCGGCGTCGGATCCCGCCGCGCCAA
CGGGCTTCGGGACACGAGAG

Downstream 100 bases:

>100_bases
CCGCTCCCGCAGCGCGGCATTTTCGCCGCGCGCCGCGACCCGAAGGACAATTCGGCCTGCGACGACTTGACAGACGGGGA
ATTCGTCTGTGTCTGTCGGC

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSIPERRVRTLFDEAAIAKRNDELAREILAARPENLLVVAVLKGSFMFAADLLRSLHRVGLAPQVEFVHLSSYRTGTVSS
GQVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMARGARRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYG
MDAAHAFRQLPFVGVVDYGDGDPDLFDA

Sequences:

>Translated_188_residues
MSIPERRVRTLFDEAAIAKRNDELAREILAARPENLLVVAVLKGSFMFAADLLRSLHRVGLAPQVEFVHLSSYRTGTVSS
GQVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMARGARRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYG
MDAAHAFRQLPFVGVVDYGDGDPDLFDA
>Mature_187_residues
SIPERRVRTLFDEAAIAKRNDELAREILAARPENLLVVAVLKGSFMFAADLLRSLHRVGLAPQVEFVHLSSYRTGTVSSG
QVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMARGARRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYGM
DAAHAFRQLPFVGVVDYGDGDPDLFDA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=176, Percent_Identity=34.0909090909091, Blast_Score=88, Evalue=4e-18,
Organism=Homo sapiens, GI9910262, Length=175, Percent_Identity=30.8571428571429, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI87081689, Length=172, Percent_Identity=39.5348837209302, Blast_Score=133, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17510817, Length=171, Percent_Identity=36.2573099415205, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20645; Mature: 20514

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPERRVRTLFDEAAIAKRNDELAREILAARPENLLVVAVLKGSFMFAADLLRSLHRVG
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
LAPQVEFVHLSSYRTGTVSSGQVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMA
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCCEEEEHHHHHHH
RGARRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYGMDAAHAFRQLPFVGVVDYGD
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHCCEEEEEECCC
GDPDLFDA
CCCCCCCC
>Mature Secondary Structure 
SIPERRVRTLFDEAAIAKRNDELAREILAARPENLLVVAVLKGSFMFAADLLRSLHRVG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
LAPQVEFVHLSSYRTGTVSSGQVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMA
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCCEEEEHHHHHHH
RGARRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYGMDAAHAFRQLPFVGVVDYGD
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHCCEEEEEECCC
GDPDLFDA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]