Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 170750341

GI number: 170750341

Start: 4196604

End: 4197539

Strand: Direct

Name: potB [H]

Synonym: Mrad2831_3944

Alternate gene names: 170750341

Gene position: 4196604-4197539 (Clockwise)

Preceding gene: 170750340

Following gene: 170750342

Centisome position: 69.05

GC content: 68.7

Gene sequence:

>936_bases
ATGACCGATCTCGCCCTGCCCGCCGCCGCGGCCGCCGAGCGGACCGCGGCGCCCGCCGTCGCGGCGCGCCCCCGCCGCCG
CGCCCTCGCCTATCTCCAGGCCGCCCCGCTGGCCCTCGTCTTCCTGATCTTCCTCGTGGTCCCGCTGGTGCTCACCGGGA
TCGTCTCGCTCTGGGAGTACAACGAGTACGAGATCATCCCGGCGCTCACCCTTCAGAACTACGCCGACGTGTTCGACGGC
TGCCTGTCGGCCGACCTCTGCACCACGGTGCGCACCTATCTCTCGACGGTGAAGTTCGTCGTGCTGACGCTGGCGATCAC
CCTGCCGCTCGGCTTCGCCGTCGCGTACTTCCTGGCCTTCCACGTCCGCTCGGGCACGGTGCGGATGGGCCTGTTCCTGC
TCTGCACGATCCCGTTCTGGACCTCGAACGTGATCCGCATGATCTCGTGGATCCCGCTGCTCGGCCGCAACGGCCTCGTC
AACGACACGCTGCGCAGCCTCGGGCTGATCCGCGCGCCGATCGAGGGCCTGCTCTACTCCGACTTCGCGGTGGTCCTGGC
ATTCGTGCACCTCGACACGGTGTTCATGATCGTGCCGATCTTCAACAGCCTCGCGCGCATCGACCGGCGGCTGATCGAGG
CGGCGCGCGACGGCGGCGCGTCCGGCGCGCAGATCCTCTGGAACGTGATCCTGCCGCTCGCCAAGCCCGGCATCGCCATC
GGGACGATCTTCGTGACGACGCTGGTGATGGGCGACTTCGTCACCGTCGGCGTCATGGGCGGCCAGCAGATCGCCAGCGT
CGGCAAGGTGATCCAGGTGCAGATGTCCTACCTGCAGTTTCCGGCCGCCGCCGCCAACGCCGTGGTGCTGCTCGCCGCGG
TGCTGCTGATGATCCTGGGGCTCACCCGGATGATCGACCTCCGGCGGGAGCTGTAG

Upstream 100 bases:

>100_bases
GAGTTCATCGCGGCGTGAGGGCGGTGGGCGCCGCCGCCTCTCCCCGCCCCCTCCCCCGCGGAGGGGGGCGGGATGCATCG
GCGCGCGGGGGGCGAATCCG

Downstream 100 bases:

>100_bases
CGATGGACCAGCCCCGCGGCCGCGCCTTCTACCTGCTGGCGGCGGTCTTCGCCCTCTACGTCCTGTTCCTCTACGGGCCG
ACCCTGACGATCCTGGCGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTDLALPAAAAAERTAAPAVAARPRRRALAYLQAAPLALVFLIFLVVPLVLTGIVSLWEYNEYEIIPALTLQNYADVFDG
CLSADLCTTVRTYLSTVKFVVLTLAITLPLGFAVAYFLAFHVRSGTVRMGLFLLCTIPFWTSNVIRMISWIPLLGRNGLV
NDTLRSLGLIRAPIEGLLYSDFAVVLAFVHLDTVFMIVPIFNSLARIDRRLIEAARDGGASGAQILWNVILPLAKPGIAI
GTIFVTTLVMGDFVTVGVMGGQQIASVGKVIQVQMSYLQFPAAAANAVVLLAAVLLMILGLTRMIDLRREL

Sequences:

>Translated_311_residues
MTDLALPAAAAAERTAAPAVAARPRRRALAYLQAAPLALVFLIFLVVPLVLTGIVSLWEYNEYEIIPALTLQNYADVFDG
CLSADLCTTVRTYLSTVKFVVLTLAITLPLGFAVAYFLAFHVRSGTVRMGLFLLCTIPFWTSNVIRMISWIPLLGRNGLV
NDTLRSLGLIRAPIEGLLYSDFAVVLAFVHLDTVFMIVPIFNSLARIDRRLIEAARDGGASGAQILWNVILPLAKPGIAI
GTIFVTTLVMGDFVTVGVMGGQQIASVGKVIQVQMSYLQFPAAAANAVVLLAAVLLMILGLTRMIDLRREL
>Mature_310_residues
TDLALPAAAAAERTAAPAVAARPRRRALAYLQAAPLALVFLIFLVVPLVLTGIVSLWEYNEYEIIPALTLQNYADVFDGC
LSADLCTTVRTYLSTVKFVVLTLAITLPLGFAVAYFLAFHVRSGTVRMGLFLLCTIPFWTSNVIRMISWIPLLGRNGLVN
DTLRSLGLIRAPIEGLLYSDFAVVLAFVHLDTVFMIVPIFNSLARIDRRLIEAARDGGASGAQILWNVILPLAKPGIAIG
TIFVTTLVMGDFVTVGVMGGQQIASVGKVIQVQMSYLQFPAAAANAVVLLAAVLLMILGLTRMIDLRREL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=246, Percent_Identity=33.739837398374, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787080, Length=249, Percent_Identity=31.3253012048193, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787713, Length=236, Percent_Identity=30.5084745762712, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33662; Mature: 33531

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLALPAAAAAERTAAPAVAARPRRRALAYLQAAPLALVFLIFLVVPLVLTGIVSLWEY
CCCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEYEIIPALTLQNYADVFDGCLSADLCTTVRTYLSTVKFVVLTLAITLPLGFAVAYFLAF
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVRSGTVRMGLFLLCTIPFWTSNVIRMISWIPLLGRNGLVNDTLRSLGLIRAPIEGLLYS
HHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DFAVVLAFVHLDTVFMIVPIFNSLARIDRRLIEAARDGGASGAQILWNVILPLAKPGIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
GTIFVTTLVMGDFVTVGVMGGQQIASVGKVIQVQMSYLQFPAAAANAVVLLAAVLLMILG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LTRMIDLRREL
HHHHHHHHHCC
>Mature Secondary Structure 
TDLALPAAAAAERTAAPAVAARPRRRALAYLQAAPLALVFLIFLVVPLVLTGIVSLWEY
CCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEYEIIPALTLQNYADVFDGCLSADLCTTVRTYLSTVKFVVLTLAITLPLGFAVAYFLAF
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVRSGTVRMGLFLLCTIPFWTSNVIRMISWIPLLGRNGLVNDTLRSLGLIRAPIEGLLYS
HHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DFAVVLAFVHLDTVFMIVPIFNSLARIDRRLIEAARDGGASGAQILWNVILPLAKPGIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
GTIFVTTLVMGDFVTVGVMGGQQIASVGKVIQVQMSYLQFPAAAANAVVLLAAVLLMILG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LTRMIDLRREL
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]