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The map label for this gene is hyuA [H]

Identifier: 170750338

GI number: 170750338

Start: 4192769

End: 4194196

Strand: Direct

Name: hyuA [H]

Synonym: Mrad2831_3941

Alternate gene names: 170750338

Gene position: 4192769-4194196 (Clockwise)

Preceding gene: 170750337

Following gene: 170750339

Centisome position: 68.98

GC content: 71.78

Gene sequence:

>1428_bases
ATGCCCGAATTCGATCTTGCCATCCGGGGCGGCACGGTGGTCACGGCGAGCGACACGGTCCGGGCCGATGTCGGCGTGCG
CGGCGGCCGGATCGTCGCGGTGGCCGAGGGCATCGCCGACGCGGCCCGGGTGATCGACGCCTCCGGCCTGCTGGTGCTGC
CCGGCGGCATCGACAGCCACGTCCACATCGCCCAGGAGTCCGGCCCCGGCATCGTCATGGCCGACGATTTCGCCAGCGCC
ACCCGGGCGGCGGCGGCCGGCGGCAACACCTGCGTGATGCCCTTCGCGCTCCAGCCCCGGGGCGCCTCCCTGCGCGGTGC
GGTCGACGCCTACAGGGCCCGGGCCGAGGGCCAGTGCCACGTCGACGTGGCGATCCACATGATCATCTCGGACCCGAGCG
AGGCGGTGCTCGGCCAGGAGCTGCCGGCTCTGGTCGCCGACGGCTACACCTCGTTCAAGGTCTTCATGACCTACGACGAC
CTCGTCCTGAACGACCGCCAGCTCCTCGACGTGTTCCACGTCGCGCGGCGGCAGGGCGCCCGGGTCATGGTCCACGCCGA
GGGCTACGACGCGATCCGCTACCTCAGCGAGCGGCTGGAGCGGGCGGGCGAGACCAGGCCCTACGGCCACGCCCTGTCGC
GCCCCGAGATCGTCGAGCGGGAGGCGGCGCACCGGGCGATCAGCCACGCCGAGCTGATCGACCTGCCGATCGTCATCGTC
CACGTCTCGGGCCGCGAGGCCACCGAGCAGATCGCCTGGGCGCAGGCGCGGGGCCTGAAGATCCGGGCCGAGACCTGCCC
GCAATACCTGACGCTGACGGCCGAGGACATGAAGGGACTCGGCCAGGAGGATCTCATGGCGGGGGCGAAGTACGTCTGCT
CGCCGCCGACGCGGGACGTCGAGAGCCAGGAGGCGGTCTGGTCGGGGATCCGGCGCGGGATCTTCGACGTGGTCTCCTCC
GACCATTGCCCGTTCCGCTACGACGACCCGCAGGGCAAGCTGAACCCGCGGGGTAAGACCTCCTTCCGCTGGATCCCGAA
CGGCATCCCCGGCATCGAGACGCGGCTGCCGGTGTTCTTCTCGGAAGGGGTCTCGAAGGGGCGGATCAGCCTCAACCAGT
TCGCCGCGCTGACCGCCACCAACCACGCCAAGCTGTACGGGCTCTACCCGCGCAAGGGCTCGATCGCTCCGGGCTTCGAC
GCCGACCTCACGCTTTGGGATCCGAACCGGCGCGAGACCATCCGCCAGGCGAACCTGCACCACGGCTCCGACTACACGCC
GTGGGAAGGCTTCGACGTCACCGGCTGGCCGGTGGTGACGATCGCGGGCGGGCAGGTGATCGCCGAGGACGGCCGGGTCC
TGTCCGAGCCGGGCCGCGGGCGGGTGCTCGACCGGGCCGTCGACCCGGCCGAGCGGCGGGACGCCTGA

Upstream 100 bases:

>100_bases
GGCCGCCTGTGACCTCCTGCCCCGCCGACCCCGTCCGCCCGAATCGGCGCGGACCGTGCATCGGCCCGGCGCGCCCGTTG
GCGCGGAAGGAGTGAGCGGC

Downstream 100 bases:

>100_bases
ACGACGCGCTGGCCCGCCGCTTGCCTGCAGCCCCCGGGGGGAGAACCGCATGGACGTCGAGCTGATCGGCCTGACCAAGC
GCTACGGCGCGGCCACCGCG

Product: phenylhydantoinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MPEFDLAIRGGTVVTASDTVRADVGVRGGRIVAVAEGIADAARVIDASGLLVLPGGIDSHVHIAQESGPGIVMADDFASA
TRAAAAGGNTCVMPFALQPRGASLRGAVDAYRARAEGQCHVDVAIHMIISDPSEAVLGQELPALVADGYTSFKVFMTYDD
LVLNDRQLLDVFHVARRQGARVMVHAEGYDAIRYLSERLERAGETRPYGHALSRPEIVEREAAHRAISHAELIDLPIVIV
HVSGREATEQIAWAQARGLKIRAETCPQYLTLTAEDMKGLGQEDLMAGAKYVCSPPTRDVESQEAVWSGIRRGIFDVVSS
DHCPFRYDDPQGKLNPRGKTSFRWIPNGIPGIETRLPVFFSEGVSKGRISLNQFAALTATNHAKLYGLYPRKGSIAPGFD
ADLTLWDPNRRETIRQANLHHGSDYTPWEGFDVTGWPVVTIAGGQVIAEDGRVLSEPGRGRVLDRAVDPAERRDA

Sequences:

>Translated_475_residues
MPEFDLAIRGGTVVTASDTVRADVGVRGGRIVAVAEGIADAARVIDASGLLVLPGGIDSHVHIAQESGPGIVMADDFASA
TRAAAAGGNTCVMPFALQPRGASLRGAVDAYRARAEGQCHVDVAIHMIISDPSEAVLGQELPALVADGYTSFKVFMTYDD
LVLNDRQLLDVFHVARRQGARVMVHAEGYDAIRYLSERLERAGETRPYGHALSRPEIVEREAAHRAISHAELIDLPIVIV
HVSGREATEQIAWAQARGLKIRAETCPQYLTLTAEDMKGLGQEDLMAGAKYVCSPPTRDVESQEAVWSGIRRGIFDVVSS
DHCPFRYDDPQGKLNPRGKTSFRWIPNGIPGIETRLPVFFSEGVSKGRISLNQFAALTATNHAKLYGLYPRKGSIAPGFD
ADLTLWDPNRRETIRQANLHHGSDYTPWEGFDVTGWPVVTIAGGQVIAEDGRVLSEPGRGRVLDRAVDPAERRDA
>Mature_474_residues
PEFDLAIRGGTVVTASDTVRADVGVRGGRIVAVAEGIADAARVIDASGLLVLPGGIDSHVHIAQESGPGIVMADDFASAT
RAAAAGGNTCVMPFALQPRGASLRGAVDAYRARAEGQCHVDVAIHMIISDPSEAVLGQELPALVADGYTSFKVFMTYDDL
VLNDRQLLDVFHVARRQGARVMVHAEGYDAIRYLSERLERAGETRPYGHALSRPEIVEREAAHRAISHAELIDLPIVIVH
VSGREATEQIAWAQARGLKIRAETCPQYLTLTAEDMKGLGQEDLMAGAKYVCSPPTRDVESQEAVWSGIRRGIFDVVSSD
HCPFRYDDPQGKLNPRGKTSFRWIPNGIPGIETRLPVFFSEGVSKGRISLNQFAALTATNHAKLYGLYPRKGSIAPGFDA
DLTLWDPNRRETIRQANLHHGSDYTPWEGFDVTGWPVVTIAGGQVIAEDGRVLSEPGRGRVLDRAVDPAERRDA

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503051, Length=469, Percent_Identity=35.181236673774, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI62422571, Length=469, Percent_Identity=35.181236673774, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI190194363, Length=469, Percent_Identity=35.3944562899787, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI4503375, Length=469, Percent_Identity=36.4605543710021, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI4503377, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=5e-68,
Organism=Homo sapiens, GI19923821, Length=460, Percent_Identity=36.0869565217391, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4503379, Length=463, Percent_Identity=33.4773218142549, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI18105007, Length=422, Percent_Identity=27.2511848341232, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI87082175, Length=462, Percent_Identity=37.4458874458874, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1786722, Length=454, Percent_Identity=31.7180616740088, Blast_Score=199, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17539558, Length=470, Percent_Identity=34.468085106383, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI71989490, Length=468, Percent_Identity=35.042735042735, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI86575075, Length=465, Percent_Identity=28.8172043010753, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI193204318, Length=421, Percent_Identity=23.7529691211401, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322218, Length=436, Percent_Identity=28.6697247706422, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI221377917, Length=471, Percent_Identity=37.3673036093418, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17137462, Length=469, Percent_Identity=34.9680170575693, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24644287, Length=299, Percent_Identity=39.4648829431438, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24644289, Length=225, Percent_Identity=41.3333333333333, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24642586, Length=419, Percent_Identity=27.9236276849642, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI18859883, Length=477, Percent_Identity=26.4150943396226, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059
- InterPro:   IPR011612 [H]

Pfam domain/function: PF01979 Amidohydro_1; PF00449 Urease_alpha [H]

EC number: 3.5.2.2

Molecular weight: Translated: 51470; Mature: 51338

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEFDLAIRGGTVVTASDTVRADVGVRGGRIVAVAEGIADAARVIDASGLLVLPGGIDSH
CCCCEEEEECCEEEEECCCEEEECCCCCCEEEEEECCHHHHHHHCCCCCEEEECCCCCCC
VHIAQESGPGIVMADDFASATRAAAAGGNTCVMPFALQPRGASLRGAVDAYRARAEGQCH
EEEEECCCCEEEEECCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEE
VDVAIHMIISDPSEAVLGQELPALVADGYTSFKVFMTYDDLVLNDRQLLDVFHVARRQGA
EEEEEEEEECCCHHHHHHHHCCHHHHCCCCEEEEEEEECCEEECCHHHHHHHHHHHHCCC
RVMVHAEGYDAIRYLSERLERAGETRPYGHALSRPEIVEREAAHRAISHAELIDLPIVIV
EEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
HVSGREATEQIAWAQARGLKIRAETCPQYLTLTAEDMKGLGQEDLMAGAKYVCSPPTRDV
EECCCHHHHHHHHHHHCCCEEECCCCCCEEEEEHHHHHCCCHHHHHHCCCEEECCCCCCC
ESQEAVWSGIRRGIFDVVSSDHCPFRYDDPQGKLNPRGKTSFRWIPNGIPGIETRLPVFF
CHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHH
SEGVSKGRISLNQFAALTATNHAKLYGLYPRKGSIAPGFDADLTLWDPNRRETIRQANLH
HCCCCCCCEEHHHEEEEEECCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCC
HGSDYTPWEGFDVTGWPVVTIAGGQVIAEDGRVLSEPGRGRVLDRAVDPAERRDA
CCCCCCCCCCCCCCCCEEEEEECCEEEECCCCEECCCCCCCCHHHCCCCHHHCCC
>Mature Secondary Structure 
PEFDLAIRGGTVVTASDTVRADVGVRGGRIVAVAEGIADAARVIDASGLLVLPGGIDSH
CCCEEEEECCEEEEECCCEEEECCCCCCEEEEEECCHHHHHHHCCCCCEEEECCCCCCC
VHIAQESGPGIVMADDFASATRAAAAGGNTCVMPFALQPRGASLRGAVDAYRARAEGQCH
EEEEECCCCEEEEECCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEE
VDVAIHMIISDPSEAVLGQELPALVADGYTSFKVFMTYDDLVLNDRQLLDVFHVARRQGA
EEEEEEEEECCCHHHHHHHHCCHHHHCCCCEEEEEEEECCEEECCHHHHHHHHHHHHCCC
RVMVHAEGYDAIRYLSERLERAGETRPYGHALSRPEIVEREAAHRAISHAELIDLPIVIV
EEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
HVSGREATEQIAWAQARGLKIRAETCPQYLTLTAEDMKGLGQEDLMAGAKYVCSPPTRDV
EECCCHHHHHHHHHHHCCCEEECCCCCCEEEEEHHHHHCCCHHHHHHCCCEEECCCCCCC
ESQEAVWSGIRRGIFDVVSSDHCPFRYDDPQGKLNPRGKTSFRWIPNGIPGIETRLPVFF
CHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHH
SEGVSKGRISLNQFAALTATNHAKLYGLYPRKGSIAPGFDADLTLWDPNRRETIRQANLH
HCCCCCCCEEHHHEEEEEECCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCC
HGSDYTPWEGFDVTGWPVVTIAGGQVIAEDGRVLSEPGRGRVLDRAVDPAERRDA
CCCCCCCCCCCCCCCCEEEEEECCEEEECCCCEECCCCCCCCHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA