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The map label for this gene is ispG [H]

Identifier: 170750328

GI number: 170750328

Start: 4180766

End: 4182085

Strand: Direct

Name: ispG [H]

Synonym: Mrad2831_3931

Alternate gene names: 170750328

Gene position: 4180766-4182085 (Clockwise)

Preceding gene: 170750319

Following gene: 170750329

Centisome position: 68.79

GC content: 70.45

Gene sequence:

>1320_bases
ATGGAAGCCACCCGTTCCGAAATCCCCGCCGACAACCCGCTGGCCGGCGGCGGCCCGGAGATCGCCGGCCCCGCCCCGCG
CCACCGCACCGTCGGCGTCACGGTCGGCGAGGGCGACGGCGCGGTCACGGTCGGCGGCGGCGCCCCGATCGTCGTCCAGT
CGATGACCAACACCGACACGGCCGACATCGACGCCACGGTTGCGCAGGTCGCCCAGCTCGCGCGGGTCGGCTCGGAGCTC
GTGCGGATCACGGTCGACCGCGACGAGGCGGCCGCCGCGGTGCCGAAGATCCGCGAGCGCCTCGACCGGATCGGCGTCCA
CGTGCCGCTGGTGGGCGACTTCCACTACATCGGCCACAAGCTGCTCGCCGACCACCCGGCCTGCGCCGAGGCGCTGGCCA
AGTACCGGATCAACCCCGGCAACGTCGGATTCAAGGACAAGAAGGACACGCAGTTCTCGTCGATCATCGAGCAGGCGATC
AAGCACAACAAGACCGTGCGGATCGGCGCCAACTGGGGCTCCCTCGACGGCGAGCTGCTCACCCACCTGATGGACGAGAA
CGCCAAGGCGCCGCGGCCGATCGACGCCCGCGCGGTGATGCGCGAGGCGATGGTCCGCTCGGCGATCAGCTCGGCCGACC
GCGCCGTGGAGCTCGGCCTGCCGCAGAACAAGATCATCCTCTCGGCCAAGGTCTCGGCGGTGCAGGACCTGATCGCCGTC
TATCGCGAGGTGGCGCGCCGCTCGGACTACGCGATCCATCTCGGCCTCACCGAGGCCGGCATGGGCTCGAAGGGCCTCGT
GGCGGCCTCGGCCGCCATCGGCGTGCTGCTCCAGGAGGGCATCGGCGACACGATCCGGTACTCGCTGACGCCCGAGCCGG
GCGGCGACCGGACCGTCGAGGTCAAGGCCAGCCAGGAACTCCTGCAGACCATGGGGTTCCGCACCTTCGTGCCGATGGTG
GCGGCCTGCCCGGGCTGCGGCCGCACGACCTCGACCACGTTCCAGGAGCTGGCCCGCGACATCCAGAACTGGATCGTCAC
CTCGATGCCGGAGTGGAAGAAGACCTATCCGGGCGTCGAGGGGCTCAACGTCGCCGTGATGGGCTGCATCGTGAACGGCC
CGGGCGAGTCGAAGCACGCCGATATCGGCATCTCGCTGCCCGGGACCGGCGAGACCCCGACCGCGCCGGTCTTCATCGAC
GGCAAGAAGGCGATGACGCTCCGCGGCCCGACGCTGGCCAAGGACTTCGAGACGATCGTCATCGACTACATCGAGCGCCG
ATTCGGCCAGCAGGGCGGCACCCGCACCGCCGCCGAGTGA

Upstream 100 bases:

>100_bases
GCCCCGAAGTTCCGGCGAGGCGTGTCCCCGGGACCGGCCCGGCAGCCGTCACGGCTCCGGCACCCGCGAGGCGCGCCCGC
GAAAGTCCACGATGGAAGTC

Downstream 100 bases:

>100_bases
GGAGCGGGGTGGGCCGACGAGCACGCCCCCCCGCGACGGAGGACGCGGCGCCCCGGACCGCGATTCCGGGTCGCCGCGGG
CGGGTCCGGATCCCGGGCGC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MEATRSEIPADNPLAGGGPEIAGPAPRHRTVGVTVGEGDGAVTVGGGAPIVVQSMTNTDTADIDATVAQVAQLARVGSEL
VRITVDRDEAAAAVPKIRERLDRIGVHVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDTQFSSIIEQAI
KHNKTVRIGANWGSLDGELLTHLMDENAKAPRPIDARAVMREAMVRSAISSADRAVELGLPQNKIILSAKVSAVQDLIAV
YREVARRSDYAIHLGLTEAGMGSKGLVAASAAIGVLLQEGIGDTIRYSLTPEPGGDRTVEVKASQELLQTMGFRTFVPMV
AACPGCGRTTSTTFQELARDIQNWIVTSMPEWKKTYPGVEGLNVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFID
GKKAMTLRGPTLAKDFETIVIDYIERRFGQQGGTRTAAE

Sequences:

>Translated_439_residues
MEATRSEIPADNPLAGGGPEIAGPAPRHRTVGVTVGEGDGAVTVGGGAPIVVQSMTNTDTADIDATVAQVAQLARVGSEL
VRITVDRDEAAAAVPKIRERLDRIGVHVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDTQFSSIIEQAI
KHNKTVRIGANWGSLDGELLTHLMDENAKAPRPIDARAVMREAMVRSAISSADRAVELGLPQNKIILSAKVSAVQDLIAV
YREVARRSDYAIHLGLTEAGMGSKGLVAASAAIGVLLQEGIGDTIRYSLTPEPGGDRTVEVKASQELLQTMGFRTFVPMV
AACPGCGRTTSTTFQELARDIQNWIVTSMPEWKKTYPGVEGLNVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFID
GKKAMTLRGPTLAKDFETIVIDYIERRFGQQGGTRTAAE
>Mature_439_residues
MEATRSEIPADNPLAGGGPEIAGPAPRHRTVGVTVGEGDGAVTVGGGAPIVVQSMTNTDTADIDATVAQVAQLARVGSEL
VRITVDRDEAAAAVPKIRERLDRIGVHVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDTQFSSIIEQAI
KHNKTVRIGANWGSLDGELLTHLMDENAKAPRPIDARAVMREAMVRSAISSADRAVELGLPQNKIILSAKVSAVQDLIAV
YREVARRSDYAIHLGLTEAGMGSKGLVAASAAIGVLLQEGIGDTIRYSLTPEPGGDRTVEVKASQELLQTMGFRTFVPMV
AACPGCGRTTSTTFQELARDIQNWIVTSMPEWKKTYPGVEGLNVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFID
GKKAMTLRGPTLAKDFETIVIDYIERRFGQQGGTRTAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=399, Percent_Identity=32.8320802005013, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46540; Mature: 46540

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATRSEIPADNPLAGGGPEIAGPAPRHRTVGVTVGEGDGAVTVGGGAPIVVQSMTNTDT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCEEEEECCCCCC
ADIDATVAQVAQLARVGSELVRITVDRDEAAAAVPKIRERLDRIGVHVPLVGDFHYIGHK
CHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHH
LLADHPACAEALAKYRINPGNVGFKDKKDTQFSSIIEQAIKHNKTVRIGANWGSLDGELL
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
THLMDENAKAPRPIDARAVMREAMVRSAISSADRAVELGLPQNKIILSAKVSAVQDLIAV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEEHHHHHHHHHHHH
YREVARRSDYAIHLGLTEAGMGSKGLVAASAAIGVLLQEGIGDTIRYSLTPEPGGDRTVE
HHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEE
VKASQELLQTMGFRTFVPMVAACPGCGRTTSTTFQELARDIQNWIVTSMPEWKKTYPGVE
EHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
GLNVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFIDGKKAMTLRGPTLAKDFETIV
CCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHH
IDYIERRFGQQGGTRTAAE
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEATRSEIPADNPLAGGGPEIAGPAPRHRTVGVTVGEGDGAVTVGGGAPIVVQSMTNTDT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCEEEEECCCCCC
ADIDATVAQVAQLARVGSELVRITVDRDEAAAAVPKIRERLDRIGVHVPLVGDFHYIGHK
CHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHH
LLADHPACAEALAKYRINPGNVGFKDKKDTQFSSIIEQAIKHNKTVRIGANWGSLDGELL
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
THLMDENAKAPRPIDARAVMREAMVRSAISSADRAVELGLPQNKIILSAKVSAVQDLIAV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEEHHHHHHHHHHHH
YREVARRSDYAIHLGLTEAGMGSKGLVAASAAIGVLLQEGIGDTIRYSLTPEPGGDRTVE
HHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEE
VKASQELLQTMGFRTFVPMVAACPGCGRTTSTTFQELARDIQNWIVTSMPEWKKTYPGVE
EHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
GLNVAVMGCIVNGPGESKHADIGISLPGTGETPTAPVFIDGKKAMTLRGPTLAKDFETIV
CCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHH
IDYIERRFGQQGGTRTAAE
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA