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The map label for this gene is ephD [H]

Identifier: 170750303

GI number: 170750303

Start: 4154785

End: 4155627

Strand: Reverse

Name: ephD [H]

Synonym: Mrad2831_3906

Alternate gene names: 170750303

Gene position: 4155627-4154785 (Counterclockwise)

Preceding gene: 170750305

Following gene: 170750302

Centisome position: 68.37

GC content: 74.02

Gene sequence:

>843_bases
ATGAGCCAGCACCCCGCGATCAGCCCCGGTCGCAACGCGGTCGTCACCGGGGCTGCGAGCGGCATCGGCCTCGCCGCCGC
GCAGGCCTTCGCCCGGGCCGGCATGAACGTCTGGCTCGCCGACCTGCCGGGCCCGGCCCTGGACGCGGCCCGAGCCGCCG
TCGCCGAACTCGCCGCCGTCGAGGTGCGCGCCGTGCCCACCGACGTGTCGGACCGCGGCGCCGTCGAGGCCCTGGCGGCC
GCCGTCGCCAAGGGCGGGCCGCCCGCCCTGGTGATGCTGAATGCCGGGATCGAGGCCGGCGGAAAGCTGTTCTCCGACGC
CGACACCTGGGCGCGGATCCTGGGCACGAATCTCGGTGGCGTGGTCAACGGCATCCACGCCTTCGCGCCCGGCATGATCG
CGGGCGGCAGGCCCGGCGCGATCGTCGTCACGGGCTCGAAGCAGGGCATCACCACCCCGCCGGGCAACGCGCCCTACAAC
GTCTCGAAGGCGGGCGTGAAGGCGGTCACCGAGGCGCTGGCTCACGAGCTGCGCAACCGGGACGGCTGCCGCACCACCGC
GCACCTGCTGATCCCGGGCTTCGTCTACACGGGCCTCACCCGGGCGCGCGGCGTCGCCGAGAAGCCCGCGGCGGCCTGGA
CGCCCGACGAGACCGTGGCCTTCATGCTGGAGCGCCTCGCGGCCGGCGACTTCTACATCCTCTGCCCCGACAACGAGACC
ACCCGGGCCCAGGACGAGAAGCGGATCGCCTGGGCGTCCGGCGACATCGTCGAGAACCGGCCCGCCCTCTCGCGCTGGCA
TCCGGACTACGCCGAGGCGTTCAAGCGGTACATGGAGGGGTGA

Upstream 100 bases:

>100_bases
CCGCTGGTACTGCCGGGGCCGGGGCCAGGATCGGGACGCGATCTCGCCTTCCTCGCGGGGGCGCCCCATCTGATGGACGC
CCGTCAGCCCAGGATCCGTC

Downstream 100 bases:

>100_bases
CCCACGCGGCCTCGCGCTGCCCTGGGTTGCTTCGCTGCGCTCGCAAGGACGGAGCGTGGCAGCGGGGGCTTGCCCGGACC
TGTCCCGGCCGTCCCCTGAA

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSQHPAISPGRNAVVTGAASGIGLAAAQAFARAGMNVWLADLPGPALDAARAAVAELAAVEVRAVPTDVSDRGAVEALAA
AVAKGGPPALVMLNAGIEAGGKLFSDADTWARILGTNLGGVVNGIHAFAPGMIAGGRPGAIVVTGSKQGITTPPGNAPYN
VSKAGVKAVTEALAHELRNRDGCRTTAHLLIPGFVYTGLTRARGVAEKPAAAWTPDETVAFMLERLAAGDFYILCPDNET
TRAQDEKRIAWASGDIVENRPALSRWHPDYAEAFKRYMEG

Sequences:

>Translated_280_residues
MSQHPAISPGRNAVVTGAASGIGLAAAQAFARAGMNVWLADLPGPALDAARAAVAELAAVEVRAVPTDVSDRGAVEALAA
AVAKGGPPALVMLNAGIEAGGKLFSDADTWARILGTNLGGVVNGIHAFAPGMIAGGRPGAIVVTGSKQGITTPPGNAPYN
VSKAGVKAVTEALAHELRNRDGCRTTAHLLIPGFVYTGLTRARGVAEKPAAAWTPDETVAFMLERLAAGDFYILCPDNET
TRAQDEKRIAWASGDIVENRPALSRWHPDYAEAFKRYMEG
>Mature_279_residues
SQHPAISPGRNAVVTGAASGIGLAAAQAFARAGMNVWLADLPGPALDAARAAVAELAAVEVRAVPTDVSDRGAVEALAAA
VAKGGPPALVMLNAGIEAGGKLFSDADTWARILGTNLGGVVNGIHAFAPGMIAGGRPGAIVVTGSKQGITTPPGNAPYNV
SKAGVKAVTEALAHELRNRDGCRTTAHLLIPGFVYTGLTRARGVAEKPAAAWTPDETVAFMLERLAAGDFYILCPDNETT
RAQDEKRIAWASGDIVENRPALSRWHPDYAEAFKRYMEG

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI20149619, Length=202, Percent_Identity=32.1782178217822, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787335, Length=197, Percent_Identity=30.9644670050761, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082100, Length=221, Percent_Identity=33.0316742081448, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=195, Percent_Identity=33.8461538461538, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=192, Percent_Identity=32.2916666666667, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI23397609, Length=213, Percent_Identity=32.3943661971831, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665243, Length=231, Percent_Identity=32.4675324675325, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24639444, Length=224, Percent_Identity=31.25, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221331218, Length=222, Percent_Identity=32.8828828828829, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357041, Length=217, Percent_Identity=29.4930875576037, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 28898; Mature: 28767

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHPAISPGRNAVVTGAASGIGLAAAQAFARAGMNVWLADLPGPALDAARAAVAELAAV
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHH
EVRAVPTDVSDRGAVEALAAAVAKGGPPALVMLNAGIEAGGKLFSDADTWARILGTNLGG
HEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHH
VVNGIHAFAPGMIAGGRPGAIVVTGSKQGITTPPGNAPYNVSKAGVKAVTEALAHELRNR
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
DGCRTTAHLLIPGFVYTGLTRARGVAEKPAAAWTPDETVAFMLERLAAGDFYILCPDNET
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
TRAQDEKRIAWASGDIVENRPALSRWHPDYAEAFKRYMEG
CCCCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQHPAISPGRNAVVTGAASGIGLAAAQAFARAGMNVWLADLPGPALDAARAAVAELAAV
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHH
EVRAVPTDVSDRGAVEALAAAVAKGGPPALVMLNAGIEAGGKLFSDADTWARILGTNLGG
HEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHH
VVNGIHAFAPGMIAGGRPGAIVVTGSKQGITTPPGNAPYNVSKAGVKAVTEALAHELRNR
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
DGCRTTAHLLIPGFVYTGLTRARGVAEKPAAAWTPDETVAFMLERLAAGDFYILCPDNET
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
TRAQDEKRIAWASGDIVENRPALSRWHPDYAEAFKRYMEG
CCCCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]