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The map label for this gene is tuf [H]

Identifier: 170750245

GI number: 170750245

Start: 4097898

End: 4099088

Strand: Reverse

Name: tuf [H]

Synonym: Mrad2831_3847

Alternate gene names: 170750245

Gene position: 4099088-4097898 (Counterclockwise)

Preceding gene: 170750247

Following gene: 170750244

Centisome position: 67.44

GC content: 67.25

Gene sequence:

>1191_bases
ATGGGCAAGGAAAAGTTCTCCCGCACCAAGCCGCACTGCAACATTGGCACGATCGGCCACGTCGATCACGGCAAGACGTC
TCTGACGGCGGCGATCACGAAGGTTCTGGCCGAGACGGGCGGGGCGACGTTCACGGCCTACGACCAGATCGACAAGGCGC
CTGAGGAGAAGGCGCGCGGGATCACGATCTCGACGGCGCACGTGGAGTACGAGACGCAGAACCGTCACTACGCGCACGTC
GACTGCCCCGGCCACGCCGACTACGTGAAGAACATGATCACGGGCGCGGCGCAGATGGACGGCGCGATCCTGGTTGTGTC
GGCGGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTGGCCCGCCAGGTCGGCGTGCCGGCGCTGGTGGTGT
TCCTCAACAAGGTCGACATGGTCGACGACGAGGAGCTGCTGGAGCTGGTCGAGCTCGAGGTGCGCGAGCTGCTGTCGAAG
TACGACTTCCCCGGCGACGACATCCCGATCACCAAGGGCTCGGCGCTGATGGCGCTCGAGGACAAGGAGCCGAAGATCGG
CAAGGAGGCGGTCCTGGCGCTGATGGCGACCGTGGACGCCTACATCCCGCAGCCGGAGCGTCCGATCGACATGCCGTTCC
TGATGCCGATCGAGGACGTGTTCTCGATCTCGGGCCGCGGCACGGTGGTGACGGGTCGCGTCGAGCGCGGCATCGTGAAG
GTGGGCGAGGAAGTCGAGATCGTCGGCATCCGGGCGACCACCAAGACGACGGTGACCGGCGTCGAGATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCGGGCGACAACGTCGGCGTGCTGCTGCGCGGCACGAAGCGCGAGGACGTGGAGCGCGGCCAGG
TGGTGTGCAAGCCGGGTTCGGTGAAGCCGCACTCGAAGTTCAAGGCCGAGGCGTACATCCTGACCAAGGAGGAGGGCGGC
CGCCACACGCCGTTCTTCACCAACTACCGGCCGCAGTTCTACTTCCGGACCACGGACGTGACCGGGATCTGCACGCTCCC
CGAGGGCACCGAGATGGTGATGCCGGGCGACAACGTGACCATGGACGTGGCGCTGATCGTGCCGGTGGCCATGGAGGAGA
AGCTGCGCTTCGCCATCCGCGAGGGCGGCCGCACCGTCGGCGCCGGCGTCGTCGCCGCCATCAACGACTGA

Upstream 100 bases:

>100_bases
ACCCTCTAAGCGGACCGCGTTTTTCGCCCCTGTCGGAACTTCGGGGCGGCTGACCAGGATTCGGGGCCCTTGCGTGGTCC
CCGCCGCAGGAATGGGAATC

Downstream 100 bases:

>100_bases
GCCCGCTGCCGGGGCGGCGCGCGCCGCCCCGCCTCTCCGGCGCCGGTCCCCGTCTCGACGGGGGCCGGCGTCGTCGTTTC
GGGGGGCAGAGCCGATCGGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MGKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSK
YDFPGDDIPITKGSALMALEDKEPKIGKEAVLALMATVDAYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVK
VGEEVEIVGIRATTKTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHSKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGICTLPEGTEMVMPGDNVTMDVALIVPVAMEEKLRFAIREGGRTVGAGVVAAIND

Sequences:

>Translated_396_residues
MGKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSK
YDFPGDDIPITKGSALMALEDKEPKIGKEAVLALMATVDAYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVK
VGEEVEIVGIRATTKTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHSKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGICTLPEGTEMVMPGDNVTMDVALIVPVAMEEKLRFAIREGGRTVGAGVVAAIND
>Mature_395_residues
GKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSKY
DFPGDDIPITKGSALMALEDKEPKIGKEAVLALMATVDAYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVKV
GEEVEIVGIRATTKTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHSKFKAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGICTLPEGTEMVMPGDNVTMDVALIVPVAMEEKLRFAIREGGRTVGAGVVAAIND

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=389, Percent_Identity=57.3264781491003, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=30.8888888888889, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=29.5871559633028, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=29.5871559633028, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4503475, Length=455, Percent_Identity=29.6703296703297, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI194018522, Length=324, Percent_Identity=29.6296296296296, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI194018520, Length=324, Percent_Identity=29.6296296296296, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI194097354, Length=324, Percent_Identity=29.6296296296296, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI46094014, Length=321, Percent_Identity=30.2180685358255, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=28.3018867924528, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI157426893, Length=287, Percent_Identity=26.4808362369338, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=31.8181818181818, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=76.9035532994924, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=76.9035532994924, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI2367247, Length=382, Percent_Identity=30.1047120418848, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI48994988, Length=276, Percent_Identity=32.2463768115942, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789108, Length=321, Percent_Identity=28.3489096573209, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.3834586466165, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=42.9305912596401, Blast_Score=327, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=463, Percent_Identity=31.9654427645788, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=463, Percent_Identity=31.9654427645788, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=444, Percent_Identity=29.5045045045045, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=28.4403669724771, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=28.2110091743119, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=27.8409090909091, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=29.5302013422819, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=39.6103896103896, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=28.2051282051282, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=394, Percent_Identity=66.751269035533, Blast_Score=539, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=448, Percent_Identity=30.8035714285714, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319594, Length=448, Percent_Identity=30.8035714285714, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=27.0655270655271, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=27.8592375366569, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=267, Percent_Identity=26.5917602996255, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=180, Percent_Identity=30.5555555555556, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=26.8595041322314, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=59.846547314578, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=59.846547314578, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.21875, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=31.140350877193, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=31.140350877193, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=31.140350877193, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=31.140350877193, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=30.7017543859649, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=30.7017543859649, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=30.8139534883721, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=30.9210526315789, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45550900, Length=335, Percent_Identity=28.955223880597, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=25.7142857142857, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=25.7142857142857, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43119; Mature: 42988

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARG
CCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSKYDFPGDDIPITKGSALMALE
EEHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEE
DKEPKIGKEAVLALMATVDAYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECHHHCCHHH
VGEEVEIVGIRATTKTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGS
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
VKPHSKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGICTLPEGTEMVMPGDNVT
CCCCCCCCEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MDVALIVPVAMEEKLRFAIREGGRTVGAGVVAAIND
EEEEEEEEHHHHHHHHHHHHHCCCEECCEEEEEECC
>Mature Secondary Structure 
GKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEEKARG
CCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSKYDFPGDDIPITKGSALMALE
EEHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEE
DKEPKIGKEAVLALMATVDAYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECHHHCCHHH
VGEEVEIVGIRATTKTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGS
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
VKPHSKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGICTLPEGTEMVMPGDNVT
CCCCCCCCEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MDVALIVPVAMEEKLRFAIREGGRTVGAGVVAAIND
EEEEEEEEHHHHHHHHHHHHHCCCEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA