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The map label for this gene is lipB [H]

Identifier: 170750203

GI number: 170750203

Start: 4047095

End: 4047826

Strand: Reverse

Name: lipB [H]

Synonym: Mrad2831_3805

Alternate gene names: 170750203

Gene position: 4047826-4047095 (Counterclockwise)

Preceding gene: 170750205

Following gene: 170750201

Centisome position: 66.6

GC content: 73.77

Gene sequence:

>732_bases
TTGGTAAACGCGCCCCGCCTCACGGTCAGCCCGCACGCGTTCCCGAAGCGGCCCGGCACCGACCCGGTCGCCGGCCCGGT
GGGCTGGCGCGTGAGCGACGCGCCGGTGGAGTACGCGGCCGCCCTCGCCTGGATGGAGGCGCGCGCGGAGGCGATCGCCC
GCGGCGCGGCGCCCGAATGCGTCTGGCTGCTGGAGCACCCGCCGCTCTACACCGCGGGCACGTCGGCCCGGCCCGAGGAT
CTCGTGGCGCCGGACCGCTTCCCGGTCCACGCGACGGGGCGGGGCGGGCAGTACACCTATCACGGCCCCGGCCAGCGGGT
GGCCTACGTGATGCTCGACCTCGACCGGCGCAGCCGCGACCTGCGCGCCTACGTGGCGAGCCTGGAGGCGTGGCTGATCG
AGACCCTGGCCGCCTTCAACGTCCGCGCCGAGCGCCGCGAGGACCGGGTCGGGGTCTGGGTGCGCCGACCCGAGAAGGGG
CCCGGCGTCGAGGACAAGATCGCGGCGATCGGCATCCGGGTCCGCCGCTGGGTCAGCTTCCACGGCGTCAGCCTGAACGT
GGAGCCCGACCTCGCGCATTTCGACGGCATCGTGCCGTGCGGCGTCCGGGAGCACGGCGTGACGAGTCTCGTCGATCTGG
GCCTGCCGGTGAGCATGGCCGAGGTCGACATGCAGCTCCGCGCCGCCTTCGAGGACATTTTCGGTGCGACCGCGATCGAG
GCGGATTTCTGA

Upstream 100 bases:

>100_bases
TCGCCGCCGGTCCGGTCCGGGCAAGCCTTCGCAGCAAGCCCTTGCAACCGCCGGCCGCGGGTGTTGAAGGCTTCGACCAT
CGACGCAGGATGGTGAACTC

Downstream 100 bases:

>100_bases
TCGGGCGCGCCGCCGAAGCCTCCGGCCGCGCCGATCAGAAATCCGCGTGCAGTCGCGAGGCGAAGAAGTGTGCCGGGCCG
CGGTCGCGGTTGTATCCGGG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MVNAPRLTVSPHAFPKRPGTDPVAGPVGWRVSDAPVEYAAALAWMEARAEAIARGAAPECVWLLEHPPLYTAGTSARPED
LVAPDRFPVHATGRGGQYTYHGPGQRVAYVMLDLDRRSRDLRAYVASLEAWLIETLAAFNVRAERREDRVGVWVRRPEKG
PGVEDKIAAIGIRVRRWVSFHGVSLNVEPDLAHFDGIVPCGVREHGVTSLVDLGLPVSMAEVDMQLRAAFEDIFGATAIE
ADF

Sequences:

>Translated_243_residues
MVNAPRLTVSPHAFPKRPGTDPVAGPVGWRVSDAPVEYAAALAWMEARAEAIARGAAPECVWLLEHPPLYTAGTSARPED
LVAPDRFPVHATGRGGQYTYHGPGQRVAYVMLDLDRRSRDLRAYVASLEAWLIETLAAFNVRAERREDRVGVWVRRPEKG
PGVEDKIAAIGIRVRRWVSFHGVSLNVEPDLAHFDGIVPCGVREHGVTSLVDLGLPVSMAEVDMQLRAAFEDIFGATAIE
ADF
>Mature_243_residues
MVNAPRLTVSPHAFPKRPGTDPVAGPVGWRVSDAPVEYAAALAWMEARAEAIARGAAPECVWLLEHPPLYTAGTSARPED
LVAPDRFPVHATGRGGQYTYHGPGQRVAYVMLDLDRRSRDLRAYVASLEAWLIETLAAFNVRAERREDRVGVWVRRPEKG
PGVEDKIAAIGIRVRRWVSFHGVSLNVEPDLAHFDGIVPCGVREHGVTSLVDLGLPVSMAEVDMQLRAAFEDIFGATAIE
ADF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=183, Percent_Identity=39.8907103825137, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI87081767, Length=141, Percent_Identity=44.6808510638298, Blast_Score=127, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323268, Length=180, Percent_Identity=30.5555555555556, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21355483, Length=180, Percent_Identity=37.2222222222222, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26611; Mature: 26611

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNAPRLTVSPHAFPKRPGTDPVAGPVGWRVSDAPVEYAAALAWMEARAEAIARGAAPEC
CCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VWLLEHPPLYTAGTSARPEDLVAPDRFPVHATGRGGQYTYHGPGQRVAYVMLDLDRRSRD
EEEECCCCEEECCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCEEEEEEEECCCCHHH
LRAYVASLEAWLIETLAAFNVRAERREDRVGVWVRRPEKGPGVEDKIAAIGIRVRRWVSF
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEE
HGVSLNVEPDLAHFDGIVPCGVREHGVTSLVDLGLPVSMAEVDMQLRAAFEDIFGATAIE
CCEEEECCCCHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEE
ADF
CCC
>Mature Secondary Structure
MVNAPRLTVSPHAFPKRPGTDPVAGPVGWRVSDAPVEYAAALAWMEARAEAIARGAAPEC
CCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VWLLEHPPLYTAGTSARPEDLVAPDRFPVHATGRGGQYTYHGPGQRVAYVMLDLDRRSRD
EEEECCCCEEECCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCEEEEEEEECCCCHHH
LRAYVASLEAWLIETLAAFNVRAERREDRVGVWVRRPEKGPGVEDKIAAIGIRVRRWVSF
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEE
HGVSLNVEPDLAHFDGIVPCGVREHGVTSLVDLGLPVSMAEVDMQLRAAFEDIFGATAIE
CCEEEECCCCHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEE
ADF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA