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The map label for this gene is sseA [H]

Identifier: 170750196

GI number: 170750196

Start: 4039815

End: 4040660

Strand: Direct

Name: sseA [H]

Synonym: Mrad2831_3798

Alternate gene names: 170750196

Gene position: 4039815-4040660 (Clockwise)

Preceding gene: 170750194

Following gene: 170750197

Centisome position: 66.47

GC content: 75.18

Gene sequence:

>846_bases
ATGGCCCAAGCGCCCCTCGTGTCACCCGAATGGCTGCACGACCGCCTCGCGGCCCCCGACATCGTCGTGCTCGACACCTC
CTGGTACCTCCCGGCCGCGGGCCGCGATCCCGAGGCCGAATTCCGCGCGGCCCACATCCCCGGCGCCCGCCGCTTCGATC
TCGACGCCATGAGCGACACCGAGTCGAGCCTGCCGCACATGCTCCCGCGGCCCGAGGTGTTCGCGGCGCGGATGCGCGCG
CTCGGTATCGGCGACGGCATGCAGATCGTGGTCTACGACGGACAGGGGCTGTTCTCGGCCCCGCGGGTCTGGTGGATGCT
CAAGGCCTTCGGCGTGCGCGACGCGCTGGTCCTGGACGGCGGCCTGCCGGCCTGGGTGGCGGCGGGCTACCCGACCGAGG
AGGGCGAGCCGCGCCCGCACGAGCGCCGGCACTTCACCGCCCGCCTCGACCACGGCGCCGTGGCGGACGCGGACGACGTC
GCCCGGGCCCTCGAGACCGGCTCGGCGCAGGTGGTCGATGCCCGCTCGGGCACGCGGTTCCGCGGCGAGGAGCCGGAGCC
CCGCCCCGGTGTCCGGCCGGGGCACATGCCCGGCGCCCGCAACCTGCACTACGCGGCCCTCCAGCGCGACGGCCGGATGA
AGAGCCCCGAGGAGCTGCAGGCCGTCTTCGCCGAGAACGGCGTCGATCCGGACCGGCCGATCGTGACCACCTGCGGCTCC
GGCGTGACCGCGGCGATCATCGCGCTGGCGCTGGAGACCATGGGCCGTCCCGCCCGGGGCCTCTACGACGGCTCCTGGTC
GGAATGGGGCGCCGACCCGGCCCGGGCGGTTGCGACGGGTTCCTGA

Upstream 100 bases:

>100_bases
CCGCTCGAACGCCGCCGCCCCGCGGCGGCCCGGACGTGCAACGCGCTGCGACCTGTGGTTCTGTGAGCCCGCAACGCGAA
CAGAGCCGGATCCGCCGACC

Downstream 100 bases:

>100_bases
GAGGCAGCCTCCCGAACCGGCCCGGCGGCCCGTCCGCTCGGGGCTGGTCACGCGTCGCCTTGAATGCTCCGTGATCGATT
TCGATATATCGAATGCGCCT

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MAQAPLVSPEWLHDRLAAPDIVVLDTSWYLPAAGRDPEAEFRAAHIPGARRFDLDAMSDTESSLPHMLPRPEVFAARMRA
LGIGDGMQIVVYDGQGLFSAPRVWWMLKAFGVRDALVLDGGLPAWVAAGYPTEEGEPRPHERRHFTARLDHGAVADADDV
ARALETGSAQVVDARSGTRFRGEEPEPRPGVRPGHMPGARNLHYAALQRDGRMKSPEELQAVFAENGVDPDRPIVTTCGS
GVTAAIIALALETMGRPARGLYDGSWSEWGADPARAVATGS

Sequences:

>Translated_281_residues
MAQAPLVSPEWLHDRLAAPDIVVLDTSWYLPAAGRDPEAEFRAAHIPGARRFDLDAMSDTESSLPHMLPRPEVFAARMRA
LGIGDGMQIVVYDGQGLFSAPRVWWMLKAFGVRDALVLDGGLPAWVAAGYPTEEGEPRPHERRHFTARLDHGAVADADDV
ARALETGSAQVVDARSGTRFRGEEPEPRPGVRPGHMPGARNLHYAALQRDGRMKSPEELQAVFAENGVDPDRPIVTTCGS
GVTAAIIALALETMGRPARGLYDGSWSEWGADPARAVATGS
>Mature_280_residues
AQAPLVSPEWLHDRLAAPDIVVLDTSWYLPAAGRDPEAEFRAAHIPGARRFDLDAMSDTESSLPHMLPRPEVFAARMRAL
GIGDGMQIVVYDGQGLFSAPRVWWMLKAFGVRDALVLDGGLPAWVAAGYPTEEGEPRPHERRHFTARLDHGAVADADDVA
RALETGSAQVVDARSGTRFRGEEPEPRPGVRPGHMPGARNLHYAALQRDGRMKSPEELQAVFAENGVDPDRPIVTTCGSG
VTAAIIALALETMGRPARGLYDGSWSEWGADPARAVATGS

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI61835204, Length=271, Percent_Identity=46.4944649446494, Blast_Score=234, Evalue=5e-62,
Organism=Homo sapiens, GI194473681, Length=271, Percent_Identity=46.4944649446494, Blast_Score=234, Evalue=5e-62,
Organism=Homo sapiens, GI194473668, Length=271, Percent_Identity=46.4944649446494, Blast_Score=234, Evalue=5e-62,
Organism=Homo sapiens, GI17402865, Length=272, Percent_Identity=42.6470588235294, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI87082121, Length=272, Percent_Identity=45.5882352941176, Blast_Score=237, Evalue=8e-64,
Organism=Escherichia coli, GI87081967, Length=295, Percent_Identity=27.4576271186441, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71997283, Length=309, Percent_Identity=29.4498381877023, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17561888, Length=288, Percent_Identity=27.7777777777778, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI115534702, Length=240, Percent_Identity=29.5833333333333, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17559150, Length=262, Percent_Identity=24.0458015267176, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543836, Length=294, Percent_Identity=25.8503401360544, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543838, Length=294, Percent_Identity=25.8503401360544, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324825, Length=286, Percent_Identity=33.2167832167832, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30329; Mature: 30198

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAPLVSPEWLHDRLAAPDIVVLDTSWYLPAAGRDPEAEFRAAHIPGARRFDLDAMSDT
CCCCCCCCHHHHHHHHCCCCEEEEECCEECCCCCCCCCCCEEEECCCCCCEECCCCCCCC
ESSLPHMLPRPEVFAARMRALGIGDGMQIVVYDGQGLFSAPRVWWMLKAFGVRDALVLDG
HHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECC
GLPAWVAAGYPTEEGEPRPHERRHFTARLDHGAVADADDVARALETGSAQVVDARSGTRF
CCCHHEECCCCCCCCCCCCHHHHCEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCC
RGEEPEPRPGVRPGHMPGARNLHYAALQRDGRMKSPEELQAVFAENGVDPDRPIVTTCGS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCC
GVTAAIIALALETMGRPARGLYDGSWSEWGADPARAVATGS
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHEECCC
>Mature Secondary Structure 
AQAPLVSPEWLHDRLAAPDIVVLDTSWYLPAAGRDPEAEFRAAHIPGARRFDLDAMSDT
CCCCCCCHHHHHHHHCCCCEEEEECCEECCCCCCCCCCCEEEECCCCCCEECCCCCCCC
ESSLPHMLPRPEVFAARMRALGIGDGMQIVVYDGQGLFSAPRVWWMLKAFGVRDALVLDG
HHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECC
GLPAWVAAGYPTEEGEPRPHERRHFTARLDHGAVADADDVARALETGSAQVVDARSGTRF
CCCHHEECCCCCCCCCCCCHHHHCEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCC
RGEEPEPRPGVRPGHMPGARNLHYAALQRDGRMKSPEELQAVFAENGVDPDRPIVTTCGS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCC
GVTAAIIALALETMGRPARGLYDGSWSEWGADPARAVATGS
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]