Click here to switch to the map view.

The map label for this gene is czcB [H]

Identifier: 170750085

GI number: 170750085

Start: 3921833

End: 3923155

Strand: Direct

Name: czcB [H]

Synonym: Mrad2831_3686

Alternate gene names: 170750085

Gene position: 3921833-3923155 (Clockwise)

Preceding gene: 170750081

Following gene: 170750086

Centisome position: 64.53

GC content: 71.35

Gene sequence:

>1323_bases
ATGGACGCCCGAAATCCGCTGGACCAGGACGAGGCCGTCACCGTGCCCGCGCGCGGGCGCCGGTGGCCCGGCCTGCTGGT
CGCCGTCGTGCTGCTCGCGCTCGCCCTCGGCGCCGCCTTCGCGTTTCCCGACCGGACCCGCGAGACCGTCGCGCACCTCG
TCGCGGCGGTGACCGGGCTGTTCGCCCCGGGCAACCCCGACCCGGGCATCGACGTCGAGAAGGCCGGTCCGGTGGAGCGG
GTGTTCCGGCCGTCCAAGGAGCAGCGCGCCGCCTTCGAGATCCTGCCTGTCGCCAACCTCGTGTTCCGGCCCGAAGGCAC
GGCCGAGGGACGCATCGCCCTCAACGAGGACGACAACGTCCCGGTGGTCTCGCCCTATGCCGGCCGGGTGACGAAGGTCC
TGGTCCGCGCCGGCGACGACGTGAAGGCCGGCGACGTGCTGCTGACGCTCGAGGCCACCGACATGGTCCAGGCGCAGAAC
GACTATCAGGCGGCCGTGAACAACCTCCAGAAGCAGCAGGCGCTCCTGAAGCTCGACCAGACCATCGCGCAGCGCCAGCA
GGAGCTGTTCCAGGCCCACGCGACGGCGCTGAAGGACTACCAGTCGGCGCAGAACGACGTCATCGCCGCCCAGAGCGACC
TGAAGACCGCCGAGGGCGCCCTCGACGCGGTGAAGAGCCGCCTGCGCCTGTTGGGCAAGACCGACGCCGAGATCGCCGCC
TTCGAGAAGAAGGGCACGATGAGCGCCGAGACGCAGATCCGGGCGCCGATCGCCGGCACGATCGTCCAGCGCAAGGTCGG
CATCGGACAGTACCTCTCCGCCTCCAGCGACCCGGTCTTCGTGATCGGCGACCTGTCGACGGTCTGGCTCGTGGCCAACG
TCCGCGAGAGCGAGATCCCGAAGATCCGCATCGGCCAGGAGGTCGAGGCGAAGGTGGCGGGCTTCGGCGCGCGGGTGTTC
AAGGCCCGGGTGAACTACATGGCCTCCAGCCTCGACCCGGCGACGCGGCGGCTCGCCGTGCGCAGCGAGGTCGACAACCC
GGACCGGGTGCTGCGGCCGGAGATGTTCGCCACCTACACGATCATCACCGGCAAGGGCGAGCCGAGCCCGAGCGTGCCGG
TGGCCGCCATCGTGTACGAGGGCGACCGCACCCACGTCTGGGTGGCCCGGCCGGACGGCGCCGTGGAGGCGCGCGACATC
AAGCTCGGCCTGATCAACGGCGACAACGCCCAGGTCGTGCAGGGCCTGCGCGCGGGCGAGCAGGTCGTCACCCGGGGGGC
CCTGTTCATCGACCGGGCGGCCACCGGCGACAAGGCGTCCTGA

Upstream 100 bases:

>100_bases
AAAGCACCAGCGACCGCATTGATGTAGGCGTCGCCCGGTCACGAATCGTACTCTCGTCACGCAGCCCGGACTCTCGGCCC
GGACCTAGAAGATCATCCGC

Downstream 100 bases:

>100_bases
GCGGGGATCCGGTCCGATGAACGCCCTGATCGCCTTCTCGCTGCGCCAGCGCGTGCTGGTGGTGCTGCTGTTCGTCGTGA
TGCTCGGCGTCGGCTACGGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MDARNPLDQDEAVTVPARGRRWPGLLVAVVLLALALGAAFAFPDRTRETVAHLVAAVTGLFAPGNPDPGIDVEKAGPVER
VFRPSKEQRAAFEILPVANLVFRPEGTAEGRIALNEDDNVPVVSPYAGRVTKVLVRAGDDVKAGDVLLTLEATDMVQAQN
DYQAAVNNLQKQQALLKLDQTIAQRQQELFQAHATALKDYQSAQNDVIAAQSDLKTAEGALDAVKSRLRLLGKTDAEIAA
FEKKGTMSAETQIRAPIAGTIVQRKVGIGQYLSASSDPVFVIGDLSTVWLVANVRESEIPKIRIGQEVEAKVAGFGARVF
KARVNYMASSLDPATRRLAVRSEVDNPDRVLRPEMFATYTIITGKGEPSPSVPVAAIVYEGDRTHVWVARPDGAVEARDI
KLGLINGDNAQVVQGLRAGEQVVTRGALFIDRAATGDKAS

Sequences:

>Translated_440_residues
MDARNPLDQDEAVTVPARGRRWPGLLVAVVLLALALGAAFAFPDRTRETVAHLVAAVTGLFAPGNPDPGIDVEKAGPVER
VFRPSKEQRAAFEILPVANLVFRPEGTAEGRIALNEDDNVPVVSPYAGRVTKVLVRAGDDVKAGDVLLTLEATDMVQAQN
DYQAAVNNLQKQQALLKLDQTIAQRQQELFQAHATALKDYQSAQNDVIAAQSDLKTAEGALDAVKSRLRLLGKTDAEIAA
FEKKGTMSAETQIRAPIAGTIVQRKVGIGQYLSASSDPVFVIGDLSTVWLVANVRESEIPKIRIGQEVEAKVAGFGARVF
KARVNYMASSLDPATRRLAVRSEVDNPDRVLRPEMFATYTIITGKGEPSPSVPVAAIVYEGDRTHVWVARPDGAVEARDI
KLGLINGDNAQVVQGLRAGEQVVTRGALFIDRAATGDKAS
>Mature_440_residues
MDARNPLDQDEAVTVPARGRRWPGLLVAVVLLALALGAAFAFPDRTRETVAHLVAAVTGLFAPGNPDPGIDVEKAGPVER
VFRPSKEQRAAFEILPVANLVFRPEGTAEGRIALNEDDNVPVVSPYAGRVTKVLVRAGDDVKAGDVLLTLEATDMVQAQN
DYQAAVNNLQKQQALLKLDQTIAQRQQELFQAHATALKDYQSAQNDVIAAQSDLKTAEGALDAVKSRLRLLGKTDAEIAA
FEKKGTMSAETQIRAPIAGTIVQRKVGIGQYLSASSDPVFVIGDLSTVWLVANVRESEIPKIRIGQEVEAKVAGFGARVF
KARVNYMASSLDPATRRLAVRSEVDNPDRVLRPEMFATYTIITGKGEPSPSVPVAAIVYEGDRTHVWVARPDGAVEARDI
KLGLINGDNAQVVQGLRAGEQVVTRGALFIDRAATGDKAS

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=344, Percent_Identity=25.8720930232558, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789041, Length=287, Percent_Identity=28.2229965156794, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786787, Length=324, Percent_Identity=25.6172839506173, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 47236; Mature: 47236

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARNPLDQDEAVTVPARGRRWPGLLVAVVLLALALGAAFAFPDRTRETVAHLVAAVTGL
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FAPGNPDPGIDVEKAGPVERVFRPSKEQRAAFEILPVANLVFRPEGTAEGRIALNEDDNV
CCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHEEEEEHHHEEECCCCCCCCEEEECCCCCC
PVVSPYAGRVTKVLVRAGDDVKAGDVLLTLEATDMVQAQNDYQAAVNNLQKQQALLKLDQ
CEECCCHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TIAQRQQELFQAHATALKDYQSAQNDVIAAQSDLKTAEGALDAVKSRLRLLGKTDAEIAA
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHEE
FEKKGTMSAETQIRAPIAGTIVQRKVGIGQYLSASSDPVFVIGDLSTVWLVANVRESEIP
ECCCCCCCCCCEECCCHHHHHHHHHCCCCHHHCCCCCCEEEEECCCEEEEEEECCCCCCC
KIRIGQEVEAKVAGFGARVFKARVNYMASSLDPATRRLAVRSEVDNPDRVLRPEMFATYT
EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCCCEEEEE
IITGKGEPSPSVPVAAIVYEGDRTHVWVARPDGAVEARDIKLGLINGDNAQVVQGLRAGE
EEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHH
QVVTRGALFIDRAATGDKAS
HHHHCCCEEEEECCCCCCCC
>Mature Secondary Structure
MDARNPLDQDEAVTVPARGRRWPGLLVAVVLLALALGAAFAFPDRTRETVAHLVAAVTGL
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FAPGNPDPGIDVEKAGPVERVFRPSKEQRAAFEILPVANLVFRPEGTAEGRIALNEDDNV
CCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHEEEEEHHHEEECCCCCCCCEEEECCCCCC
PVVSPYAGRVTKVLVRAGDDVKAGDVLLTLEATDMVQAQNDYQAAVNNLQKQQALLKLDQ
CEECCCHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TIAQRQQELFQAHATALKDYQSAQNDVIAAQSDLKTAEGALDAVKSRLRLLGKTDAEIAA
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHEE
FEKKGTMSAETQIRAPIAGTIVQRKVGIGQYLSASSDPVFVIGDLSTVWLVANVRESEIP
ECCCCCCCCCCEECCCHHHHHHHHHCCCCHHHCCCCCCEEEEECCCEEEEEEECCCCCCC
KIRIGQEVEAKVAGFGARVFKARVNYMASSLDPATRRLAVRSEVDNPDRVLRPEMFATYT
EEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCCCEEEEE
IITGKGEPSPSVPVAAIVYEGDRTHVWVARPDGAVEARDIKLGLINGDNAQVVQGLRAGE
EEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHH
QVVTRGALFIDRAATGDKAS
HHHHCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]