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The map label for this gene is mtkB [H]

Identifier: 170750064

GI number: 170750064

Start: 3896327

End: 3897217

Strand: Reverse

Name: mtkB [H]

Synonym: Mrad2831_3665

Alternate gene names: 170750064

Gene position: 3897217-3896327 (Counterclockwise)

Preceding gene: 170750065

Following gene: 170750063

Centisome position: 64.12

GC content: 69.47

Gene sequence:

>891_bases
ATGAGCATCCTCATCGACGAGACCACCCCGATCATCGTCCAGGGCATCACGGGCGACAAGGGCACCTTCCACGCCAAGGA
GATGCTCGACTACGGCACGAACGTCGTCGGCGGCGTCACCCCGGGCAAGGGTGGGCGCACCCATCTCGGCCTGCCGGTGT
TCAACACCGTCAAGGAAGCGGTGGCGGCGACCGGGGCCACGACCTCGATCACCTTCGTGGCGCCCCCCTTCGCGGCCGAC
GCGATCATGGAGGCGGCCGATGCCGGGCTGCGGCTGGTCTGCTCGATCACCGACGGGATCCCCGCCCAGGACATGATGCG
GGTGAAGCGCTACCTCTACCGCTATCCCAAGGAGAAGCGGACGATGGTGGTCGGCCCGAACTGCGCCGGCATCATCTCGC
CCGGCAAGGCCATGCTCGGCATCATGCCCGGCCACATCTACCTCCCAGGCAATGTCGGGGTGATCTCGCGCTCGGGCACG
CTCGGCTACGAGGCGGCCTCCCAGCTCAAGGAGCTGGGGCTCGGGATCTCGACCTCGGTGGGCATCGGCGGCGACCCGAT
CAACGGCTCGTCCTTCCTCGACCACCTCGCCCTGTTCGAGACCGACCCGGACACCGACGCGGTGCTGATGATCGGCGAGA
TCGGCGGCCCGCAGGAGGCCGAGGCCTCGGCCTGGATCCGCGAGAACATGTCGAAGCCGGTGATCGGGTTCGTGGCCGGA
CTCACGGCTCCGAAGGGGCGCCGCATGGGGCACGCCGGCGCGATCATCTCGGCGGCCGGCGACAGCGCCGCCGAGAAGGC
CGAGATCATGCGCTCGTACGGGCTGACCGTAGCGCCGGATCCGGGCTCGTTCGGCCAGACGGTGGCCGACGTGCTCGCCC
GGGCGGCGTAG

Upstream 100 bases:

>100_bases
CTGGATCTCATCACCGCCGACACCCTGGCGGAGGCGGCCAAGAAGGCGGTCGACGCCTGCGCCGCCGCCAAGACCCAGCA
CTGAGAGGGGAGACCGCGCC

Downstream 100 bases:

>100_bases
CCGCGCTGCACCCCTTCCCCCTCTGCGGGGGAGGGTGCCCCCGGATGCGCGGCGGGAGAGGGGCAGCGCGACGGTGCGGG
CTTTCAGCTCCCGTCGCGAC

Product: succinyl-CoA synthetase subunit alpha

Products: NA

Alternate protein names: MTK-alpha; Malate thiokinase; Malyl-CoA synthetase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSILIDETTPIIVQGITGDKGTFHAKEMLDYGTNVVGGVTPGKGGRTHLGLPVFNTVKEAVAATGATTSITFVAPPFAAD
AIMEAADAGLRLVCSITDGIPAQDMMRVKRYLYRYPKEKRTMVVGPNCAGIISPGKAMLGIMPGHIYLPGNVGVISRSGT
LGYEAASQLKELGLGISTSVGIGGDPINGSSFLDHLALFETDPDTDAVLMIGEIGGPQEAEASAWIRENMSKPVIGFVAG
LTAPKGRRMGHAGAIISAAGDSAAEKAEIMRSYGLTVAPDPGSFGQTVADVLARAA

Sequences:

>Translated_296_residues
MSILIDETTPIIVQGITGDKGTFHAKEMLDYGTNVVGGVTPGKGGRTHLGLPVFNTVKEAVAATGATTSITFVAPPFAAD
AIMEAADAGLRLVCSITDGIPAQDMMRVKRYLYRYPKEKRTMVVGPNCAGIISPGKAMLGIMPGHIYLPGNVGVISRSGT
LGYEAASQLKELGLGISTSVGIGGDPINGSSFLDHLALFETDPDTDAVLMIGEIGGPQEAEASAWIRENMSKPVIGFVAG
LTAPKGRRMGHAGAIISAAGDSAAEKAEIMRSYGLTVAPDPGSFGQTVADVLARAA
>Mature_295_residues
SILIDETTPIIVQGITGDKGTFHAKEMLDYGTNVVGGVTPGKGGRTHLGLPVFNTVKEAVAATGATTSITFVAPPFAADA
IMEAADAGLRLVCSITDGIPAQDMMRVKRYLYRYPKEKRTMVVGPNCAGIISPGKAMLGIMPGHIYLPGNVGVISRSGTL
GYEAASQLKELGLGISTSVGIGGDPINGSSFLDHLALFETDPDTDAVLMIGEIGGPQEAEASAWIRENMSKPVIGFVAGL
TAPKGRRMGHAGAIISAAGDSAAEKAEIMRSYGLTVAPDPGSFGQTVADVLARAA

Specific function: Unknown

COG id: COG0074

COG function: function code C; Succinyl-CoA synthetase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the succinate/malate CoA ligase alpha subunit family [H]

Homologues:

Organism=Homo sapiens, GI109452591, Length=290, Percent_Identity=53.7931034482759, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI38569423, Length=261, Percent_Identity=27.9693486590038, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI38569421, Length=261, Percent_Identity=27.9693486590038, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1786949, Length=292, Percent_Identity=55.8219178082192, Blast_Score=335, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17550194, Length=292, Percent_Identity=51.027397260274, Blast_Score=276, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI17553126, Length=295, Percent_Identity=48.4745762711864, Blast_Score=272, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17557344, Length=254, Percent_Identity=27.1653543307087, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17551266, Length=254, Percent_Identity=26.3779527559055, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324716, Length=301, Percent_Identity=46.1794019933555, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI21356559, Length=297, Percent_Identity=45.1178451178451, Blast_Score=272, Evalue=2e-73,
Organism=Drosophila melanogaster, GI28574296, Length=297, Percent_Identity=46.4646464646465, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI281363475, Length=317, Percent_Identity=24.9211356466877, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281363473, Length=317, Percent_Identity=24.9211356466877, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281363477, Length=317, Percent_Identity=24.9211356466877, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017440
- InterPro:   IPR003781
- InterPro:   IPR005810
- InterPro:   IPR005811
- InterPro:   IPR016040
- InterPro:   IPR016102 [H]

Pfam domain/function: PF02629 CoA_binding; PF00549 Ligase_CoA [H]

EC number: =6.2.1.9 [H]

Molecular weight: Translated: 30515; Mature: 30384

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01216 SUCCINYL_COA_LIG_1 ; PS00399 SUCCINYL_COA_LIG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILIDETTPIIVQGITGDKGTFHAKEMLDYGTNVVGGVTPGKGGRTHLGLPVFNTVKEA
CEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCEECCCCCCCCCCEEECCCHHHHHHHH
VAATGATTSITFVAPPFAADAIMEAADAGLRLVCSITDGIPAQDMMRVKRYLYRYPKEKR
HHHCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
TMVVGPNCAGIISPGKAMLGIMPGHIYLPGNVGVISRSGTLGYEAASQLKELGLGISTSV
EEEECCCCCEEECCCCEEEEECCCEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCCCCC
GIGGDPINGSSFLDHLALFETDPDTDAVLMIGEIGGPQEAEASAWIRENMSKPVIGFVAG
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHH
LTAPKGRRMGHAGAIISAAGDSAAEKAEIMRSYGLTVAPDPGSFGQTVADVLARAA
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SILIDETTPIIVQGITGDKGTFHAKEMLDYGTNVVGGVTPGKGGRTHLGLPVFNTVKEA
EEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCEECCCCCCCCCCEEECCCHHHHHHHH
VAATGATTSITFVAPPFAADAIMEAADAGLRLVCSITDGIPAQDMMRVKRYLYRYPKEKR
HHHCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
TMVVGPNCAGIISPGKAMLGIMPGHIYLPGNVGVISRSGTLGYEAASQLKELGLGISTSV
EEEECCCCCEEECCCCEEEEECCCEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCCCCC
GIGGDPINGSSFLDHLALFETDPDTDAVLMIGEIGGPQEAEASAWIRENMSKPVIGFVAG
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHH
LTAPKGRRMGHAGAIISAAGDSAAEKAEIMRSYGLTVAPDPGSFGQTVADVLARAA
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961516 [H]