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The map label for this gene is amtB [H]

Identifier: 170750025

GI number: 170750025

Start: 3851308

End: 3852807

Strand: Reverse

Name: amtB [H]

Synonym: Mrad2831_3626

Alternate gene names: 170750025

Gene position: 3852807-3851308 (Counterclockwise)

Preceding gene: 170750026

Following gene: 170750023

Centisome position: 63.39

GC content: 68.13

Gene sequence:

>1500_bases
ATGAAACTTCGTCATGCCCTCATGCTCGGCTTGGGAGGGGCGGCGATCGCCGCCCTCCTGATCGAGCCGTCCCTCGCGCA
GACCCCGACACCGGAAGCCGCCTCCCCGCCGCCCTCGCCGATCCCCAACAAGGGTGACACGGCCTGGATGATGATCTCGT
CGGCCCTGGTGCTGATGATGAGCATCCCCGGCCTCGCGCTGTTCTACGGCGGCCTCGTGCGCACCAAGAACATGCTGTCG
GTGCTGACCCAGGTGTTCGCGATCGTGTCGATCGTCGGCCTCCTCTGGGTGTTCTACGGCTACAGCCTCGCCTTCACGAG
CGGCGGCGGCCTCAACGACTTCGTCGGCGGCTTCTCGAAGGCGTTCCTGAAGGGCGTCGACGCGAACTCGACGGTCGCGA
CCTTCTCGAACGGCGTCGTGATCCCGGAATACGCCTATCTGTGCTTCCAGATGACGTTCGCCATGATCACGCCCGCGCTG
ATCGTCGGCGCCTTCGCCGAGCGGATGAAGTTCTCCGCGCTGGTGGTGTTCACGATCCTCTGGGTCACGATCATCTACTT
CCCGATGGCCCACATGGTCTGGTACTGGGCCGGCCCGGACGCCGTCGGCAACGCCGCCAAGGCTCTGGCCGCCGCCACCG
ACGACGCCTCGAAGGCGACCGCCCAGGCGGCCCTCGACGCGGTCAACAAGGATGCCGGCTTCCTCTTCCAGAAGGGCGCC
CTCGACTTCGCCGGCGGCACCGTGGTGCACATCAACGCGGGCATCGCCGGCTTCGTCGGCTGCCTGATCATGGGCAAGCG
CATCGGCTACGGCCGTGACCTGCTCGCCCCGCACTCCCTGACCATGACGATGATCGGCGCCTCGCTGCTGTGGGTCGGCT
GGTTCGGCTTCAACGCCGGCTCGAACCTCGAGTCCAACGGCACCGCCGCGCTCGCGATGCTCAACACCTTCGTGGCGACC
TGCGGCGCAGCGGTGGCGTGGCTGTTCGCCGAGTGGGCCCTCAAGGGCAAGCCGTCCCTGCTCGGCATGCTGTCGGGCGC
GGTCGCCGGCCTCGTCGCGGTCACCCCGGCCTCCGGCTTCGCCGGCCCGATGGGCTCGATCGTGCTGGGCCTCGTCGCCG
GCGTGGTCTGCTTCGTGATGTGCTCCACCGTGAAGAACGCGCTGGGCTACGACGACTCGCTGGACGTCTTCGGCGTCCAC
TGCGTCGGCGGCATCCTGGGCGCGCTGGCGACCGGCATCCTGGTCGACCCGAACCTCGGCGGCGTCGGCATCCCCGACTA
CACCTCGAAGCCGGGCGAGCTGGCCGTCGGTGCCTACGACATGATGAGCCAGCTCATCGTCCAGGCCGAGGCGGTCGGCC
TGACCCTGCTGTGGTCGGGCATCGGCTCGGCGATCCTCTACAAGCTCGTCGACGTGACGATCGGCCTGCGCGTCACGCAG
GAGGAGGAGCGCGAGGGTCTCGACATCGCCGATCACGGCGAGCGCGCCTACAACTACTGA

Upstream 100 bases:

>100_bases
CGACGCCGACGCCCTCTGATCCGCGGCCCGGCGCGTCCGGGCCCGCCGATCGCCAGACCTCCTGAAACAATACGGACTTG
AGACCGGGAGCCACGTGCCC

Downstream 100 bases:

>100_bases
GACGGACGGCGCGGCCCTGCCGGGCCGCGTCTCCCGAACGAGAAAACCCCGGCTCCGCGAGGGCCGGGGTTTTCCTTTGC
GTCAGGCCGTCTCGCTTCGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKLRHALMLGLGGAAIAALLIEPSLAQTPTPEAASPPPSPIPNKGDTAWMMISSALVLMMSIPGLALFYGGLVRTKNMLS
VLTQVFAIVSIVGLLWVFYGYSLAFTSGGGLNDFVGGFSKAFLKGVDANSTVATFSNGVVIPEYAYLCFQMTFAMITPAL
IVGAFAERMKFSALVVFTILWVTIIYFPMAHMVWYWAGPDAVGNAAKALAAATDDASKATAQAALDAVNKDAGFLFQKGA
LDFAGGTVVHINAGIAGFVGCLIMGKRIGYGRDLLAPHSLTMTMIGASLLWVGWFGFNAGSNLESNGTAALAMLNTFVAT
CGAAVAWLFAEWALKGKPSLLGMLSGAVAGLVAVTPASGFAGPMGSIVLGLVAGVVCFVMCSTVKNALGYDDSLDVFGVH
CVGGILGALATGILVDPNLGGVGIPDYTSKPGELAVGAYDMMSQLIVQAEAVGLTLLWSGIGSAILYKLVDVTIGLRVTQ
EEEREGLDIADHGERAYNY

Sequences:

>Translated_499_residues
MKLRHALMLGLGGAAIAALLIEPSLAQTPTPEAASPPPSPIPNKGDTAWMMISSALVLMMSIPGLALFYGGLVRTKNMLS
VLTQVFAIVSIVGLLWVFYGYSLAFTSGGGLNDFVGGFSKAFLKGVDANSTVATFSNGVVIPEYAYLCFQMTFAMITPAL
IVGAFAERMKFSALVVFTILWVTIIYFPMAHMVWYWAGPDAVGNAAKALAAATDDASKATAQAALDAVNKDAGFLFQKGA
LDFAGGTVVHINAGIAGFVGCLIMGKRIGYGRDLLAPHSLTMTMIGASLLWVGWFGFNAGSNLESNGTAALAMLNTFVAT
CGAAVAWLFAEWALKGKPSLLGMLSGAVAGLVAVTPASGFAGPMGSIVLGLVAGVVCFVMCSTVKNALGYDDSLDVFGVH
CVGGILGALATGILVDPNLGGVGIPDYTSKPGELAVGAYDMMSQLIVQAEAVGLTLLWSGIGSAILYKLVDVTIGLRVTQ
EEEREGLDIADHGERAYNY
>Mature_499_residues
MKLRHALMLGLGGAAIAALLIEPSLAQTPTPEAASPPPSPIPNKGDTAWMMISSALVLMMSIPGLALFYGGLVRTKNMLS
VLTQVFAIVSIVGLLWVFYGYSLAFTSGGGLNDFVGGFSKAFLKGVDANSTVATFSNGVVIPEYAYLCFQMTFAMITPAL
IVGAFAERMKFSALVVFTILWVTIIYFPMAHMVWYWAGPDAVGNAAKALAAATDDASKATAQAALDAVNKDAGFLFQKGA
LDFAGGTVVHINAGIAGFVGCLIMGKRIGYGRDLLAPHSLTMTMIGASLLWVGWFGFNAGSNLESNGTAALAMLNTFVAT
CGAAVAWLFAEWALKGKPSLLGMLSGAVAGLVAVTPASGFAGPMGSIVLGLVAGVVCFVMCSTVKNALGYDDSLDVFGVH
CVGGILGALATGILVDPNLGGVGIPDYTSKPGELAVGAYDMMSQLIVQAEAVGLTLLWSGIGSAILYKLVDVTIGLRVTQ
EEEREGLDIADHGERAYNY

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=475, Percent_Identity=46.5263157894737, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17550182, Length=480, Percent_Identity=27.7083333333333, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=475, Percent_Identity=28, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=459, Percent_Identity=24.1830065359477, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=494, Percent_Identity=23.8866396761134, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325396, Length=462, Percent_Identity=31.8181818181818, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321559, Length=460, Percent_Identity=30.4347826086957, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324187, Length=467, Percent_Identity=30.1927194860814, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI161078314, Length=458, Percent_Identity=22.707423580786, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51789; Mature: 51789

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRHALMLGLGGAAIAALLIEPSLAQTPTPEAASPPPSPIPNKGDTAWMMISSALVLMM
CCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SIPGLALFYGGLVRTKNMLSVLTQVFAIVSIVGLLWVFYGYSLAFTSGGGLNDFVGGFSK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHH
AFLKGVDANSTVATFSNGVVIPEYAYLCFQMTFAMITPALIVGAFAERMKFSALVVFTIL
HHHHCCCCCCCEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVTIIYFPMAHMVWYWAGPDAVGNAAKALAAATDDASKATAQAALDAVNKDAGFLFQKGA
HHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCC
LDFAGGTVVHINAGIAGFVGCLIMGKRIGYGRDLLAPHSLTMTMIGASLLWVGWFGFNAG
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
SNLESNGTAALAMLNTFVATCGAAVAWLFAEWALKGKPSLLGMLSGAVAGLVAVTPASGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCC
AGPMGSIVLGLVAGVVCFVMCSTVKNALGYDDSLDVFGVHCVGGILGALATGILVDPNLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCC
GVGIPDYTSKPGELAVGAYDMMSQLIVQAEAVGLTLLWSGIGSAILYKLVDVTIGLRVTQ
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECC
EEEREGLDIADHGERAYNY
HHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLRHALMLGLGGAAIAALLIEPSLAQTPTPEAASPPPSPIPNKGDTAWMMISSALVLMM
CCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SIPGLALFYGGLVRTKNMLSVLTQVFAIVSIVGLLWVFYGYSLAFTSGGGLNDFVGGFSK
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHH
AFLKGVDANSTVATFSNGVVIPEYAYLCFQMTFAMITPALIVGAFAERMKFSALVVFTIL
HHHHCCCCCCCEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVTIIYFPMAHMVWYWAGPDAVGNAAKALAAATDDASKATAQAALDAVNKDAGFLFQKGA
HHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCC
LDFAGGTVVHINAGIAGFVGCLIMGKRIGYGRDLLAPHSLTMTMIGASLLWVGWFGFNAG
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
SNLESNGTAALAMLNTFVATCGAAVAWLFAEWALKGKPSLLGMLSGAVAGLVAVTPASGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCC
AGPMGSIVLGLVAGVVCFVMCSTVKNALGYDDSLDVFGVHCVGGILGALATGILVDPNLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCC
GVGIPDYTSKPGELAVGAYDMMSQLIVQAEAVGLTLLWSGIGSAILYKLVDVTIGLRVTQ
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECC
EEEREGLDIADHGERAYNY
HHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]