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The map label for this gene is trmD [H]

Identifier: 170750022

GI number: 170750022

Start: 3848869

End: 3849633

Strand: Direct

Name: trmD [H]

Synonym: Mrad2831_3623

Alternate gene names: 170750022

Gene position: 3848869-3849633 (Clockwise)

Preceding gene: 170750020

Following gene: 170750024

Centisome position: 63.33

GC content: 76.08

Gene sequence:

>765_bases
ATGACGCGCGCGGACACGCCCTGGCGGGCCACGATCCTCACCCTCTACCCGGAGATGTTCCCGGGCCCGCTGGGCCTCTC
CCTGTCGGGCGACGCCTTGGCGCGGGGCGACTGGACCCTGGAGGCCCGCAACATCCGCGAGCACGGCCTCGGCCGCCACC
GCTCGGTCGACGACACGCCGGCTGGCGGCGGGGCCGGGATGGTCCTGCGCTGCGACGTGCTCGCAGCCGCCATCGACGCC
GCGGCCGTGGACGCCGCGACCGGGGCCGACGACCCGCGCCCGCGCCTGCTCATGTCGCCCCGCGGCCGGCCGCTCACCCA
GGCGCGCGTGCGCGACCTCGCGGCGGGACCGGGCGCGCTGATCGTCTGCGGCCGCTTCGAGGGCGTCGACGAGCGCGTGA
TCGCCGGGCGCGGCCTGGAGGAGGTCTCGATCGGCGACTACGTGCTGTCCGGCGGCGAGATCGCCGCCCTGGTGGTGCTG
GATGCGTGCGTGCGGCTCCTGCCCGGCGTGATGGGCAAGATGGAATCCGGCGTCGAGGAGAGCTTCGAGGGCGGGCTCCT
CGAGTACCCGCACTACACGCGCCCCCGCGACTGGGAGGGCCGCGCGATCCCGGACGTCCTGTCCAGCGGCAACCACGCCG
CCATCGCCCGCTGGCGGCGGGCGGAGGCCGAGCGGATCACCGCCGAGCGCCGCCCCGATCTGCGCGACGGGTCTGTGGCC
GGTCAGGCCGGCTCGAGGTCGATCACCGGGCCGTCGCGGTCGTAA

Upstream 100 bases:

>100_bases
CGAGGAGGGCGAGGGCGCGGATCATGGCCGACAACCCGCGGGGCGCGTCCGCGGTGCCGGGCCCGTTGACCGTGCCGCAC
CCCGCGCGCCATCAAGCGCC

Downstream 100 bases:

>100_bases
TCCAGCGGGTCGAGCTGCAGCGGCCGCGGCACGACGCGCGGTGTCCGGCTCGCCAGCACGATGCGGGGTATTAAGGCCCC
AATAACCAAGAGTAGAACGG

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTRADTPWRATILTLYPEMFPGPLGLSLSGDALARGDWTLEARNIREHGLGRHRSVDDTPAGGGAGMVLRCDVLAAAIDA
AAVDAATGADDPRPRLLMSPRGRPLTQARVRDLAAGPGALIVCGRFEGVDERVIAGRGLEEVSIGDYVLSGGEIAALVVL
DACVRLLPGVMGKMESGVEESFEGGLLEYPHYTRPRDWEGRAIPDVLSSGNHAAIARWRRAEAERITAERRPDLRDGSVA
GQAGSRSITGPSRS

Sequences:

>Translated_254_residues
MTRADTPWRATILTLYPEMFPGPLGLSLSGDALARGDWTLEARNIREHGLGRHRSVDDTPAGGGAGMVLRCDVLAAAIDA
AAVDAATGADDPRPRLLMSPRGRPLTQARVRDLAAGPGALIVCGRFEGVDERVIAGRGLEEVSIGDYVLSGGEIAALVVL
DACVRLLPGVMGKMESGVEESFEGGLLEYPHYTRPRDWEGRAIPDVLSSGNHAAIARWRRAEAERITAERRPDLRDGSVA
GQAGSRSITGPSRS
>Mature_253_residues
TRADTPWRATILTLYPEMFPGPLGLSLSGDALARGDWTLEARNIREHGLGRHRSVDDTPAGGGAGMVLRCDVLAAAIDAA
AVDAATGADDPRPRLLMSPRGRPLTQARVRDLAAGPGALIVCGRFEGVDERVIAGRGLEEVSIGDYVLSGGEIAALVVLD
ACVRLLPGVMGKMESGVEESFEGGLLEYPHYTRPRDWEGRAIPDVLSSGNHAAIARWRRAEAERITAERRPDLRDGSVAG
QAGSRSITGPSRS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=235, Percent_Identity=42.1276595744681, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26970; Mature: 26839

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRADTPWRATILTLYPEMFPGPLGLSLSGDALARGDWTLEARNIREHGLGRHRSVDDTP
CCCCCCCCEEEHEEECHHHCCCCCCCCCCCCEEECCCEEEEECCHHHHCCCCCCCCCCCC
AGGGAGMVLRCDVLAAAIDAAAVDAATGADDPRPRLLMSPRGRPLTQARVRDLAAGPGAL
CCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCEE
IVCGRFEGVDERVIAGRGLEEVSIGDYVLSGGEIAALVVLDACVRLLPGVMGKMESGVEE
EEEECCCCCCHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFEGGLLEYPHYTRPRDWEGRAIPDVLSSGNHAAIARWRRAEAERITAERRPDLRDGSVA
HHCCCCEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GQAGSRSITGPSRS
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TRADTPWRATILTLYPEMFPGPLGLSLSGDALARGDWTLEARNIREHGLGRHRSVDDTP
CCCCCCCEEEHEEECHHHCCCCCCCCCCCCEEECCCEEEEECCHHHHCCCCCCCCCCCC
AGGGAGMVLRCDVLAAAIDAAAVDAATGADDPRPRLLMSPRGRPLTQARVRDLAAGPGAL
CCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCEE
IVCGRFEGVDERVIAGRGLEEVSIGDYVLSGGEIAALVVLDACVRLLPGVMGKMESGVEE
EEEECCCCCCHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFEGGLLEYPHYTRPRDWEGRAIPDVLSSGNHAAIARWRRAEAERITAERRPDLRDGSVA
HHCCCCEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GQAGSRSITGPSRS
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]