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The map label for this gene is emrB [H]

Identifier: 170750013

GI number: 170750013

Start: 3840679

End: 3842343

Strand: Direct

Name: emrB [H]

Synonym: Mrad2831_3614

Alternate gene names: 170750013

Gene position: 3840679-3842343 (Clockwise)

Preceding gene: 170750012

Following gene: 170750019

Centisome position: 63.19

GC content: 68.65

Gene sequence:

>1665_bases
GTGAGCGCCGTGGCCGGCCAGGCGAGCGCGGCCGCGCCCGGACGCAAGCCGGGTGCGGGGCAGGCGAGAGGAAGCAAGGC
CGCGTCCGGCAAGGCCCCGGGCGGCCACAATCCGTGGCTGATCGCGGGCGTGGTGGCGCTGGCGACCTTCATGGAGGTGC
TCGACACCACCATCGCCAACGTGGCCCTGCGCTACATCTCGGGCGGGCTCGCGGTCGGCCCCGACGAGGCGGCCTGGGTG
GTGACGAGCTACCTCGTCGCCAACGCGATCACCCTGACCGCGTCGAGCTTCCTGGCCAAGCGCTACGGCCGCAAGGCCTT
CTTCATGTGGTCGGTGGCGCTGTTCACCGCCTCGTCGATCCTGTGCGGCTTCGCCTGGAGCCTGGAATCCCTGCTGCTGT
TCCGGGTGATGCAGGGGCTCGGCGGCGGCGGCATGGCGCCGCTCGCGCAGTCGATCCTGGCCGATTCCTTCCCGCCGGCG
AAGCGCGGCCAGGCCTTCGCGCTCTACGGGGTCGCCATCGTGGTGGCGCCGGTGATCGGCCCGACGCTCGGCGGCTGGCT
CTCCGACAACGCTTCCTGGCACTGGTGCTTCCTGATCAACGGGCCGGTGGGCGTGATCGCCCTCGGGCTGATGTACTGGC
TGATCGAGGACAGCGACGCGGCCAAGAAGGAGCGCCGCGCCCTGGTCCGCAAGGGCATCCGCTTCGACATCGTCGGCTTC
GTGCTGGTCGCCCTGTTCCTCGGCTCGCTGGAGGTGATCCTCGACGAGGGCCAGCGGAAGGACTGGTTCGGCACCTCGAC
GCTGATGAACGTCTCCGGCGTGCTGATGGTGGTCTCGTTCATCGCCATGATCCCGTGGCTGCTGACCCGCGAGAACCCGG
TCGTCGACCTCCGCCTGATCGGCAACCGCCAGTTCGGGTCGTGCTTCCTGGTGATGATGTTCACCGGGGCGATCCTGATC
GCCACCACGCAGTTCATCCCGCAGATCACGCAGGAATATTACGGCTACACCGCCACCCTGTCGGGCCTCGTCCTCGGCCC
GGGCGGCATCGTCACGGTGGTGATGATGTTCCTCACCGGCCGGATCTCGTCCTACGTCCAGCCGAAATGGCTGATCGCCA
CCGGCATGACGATCGTGGCGCTCGGCATGTACGACCTGACCCGGATCGACGGCAGCACGACCTTCGCGTTCTTCGCGTGG
TCGCGGGTCTATATCGGCATCGGCCTGCCGATGGTGTTCCTGTCGATCACCTCGGCCTCGTACGAGGGCATCCGCAAGGA
CCAGACCGACCAGGCCTCGGCGCTGATCAACGTCGCCCGCAACGTCGGCGGCTCGATGGGCGTCTCCCTGGCGCAGAACA
TCCTGGCCTACCGGAGCCAGTTCCATCAGAGCCGCCTGGTGGAGAGCGTCAACCCGGCCTCGCCGGCCTACCAGGAGACC
ATGCGGCAGGCGACGCAGTACTTCTCCGAGCACGGCTACGCCGGTGTCGAGGCCCAGAACCAGGCGACGGCGTGGATCGG
GAGCGTGCTCAGCACCCAGGTGGCCTACTGGGCCTATATCGACGTGTTCTGGGTGCTCGGCCTCGTCGCCGCCTGCGCGG
TCCCGCTGGCCCTGAGCCTGAAGAGCGTCAAGCTCGGCGGTGGGGCGCCGGCCGGGGGGCACTGA

Upstream 100 bases:

>100_bases
AGGTGACCCAGCGCATCCCGGTCAAGATCGTGGTCAAGAACTGGCCGACGGACGTGTCGATCGGCCCGGGCATGTCGATC
GTCCCGACCGTCACGGTGCG

Downstream 100 bases:

>100_bases
GGGCGGCGGCCGCGGCCGGGACGGCGCCCGGATTCCGGACGCGCCCTCGGCGCGCGGACCGGCCCCCGAGCCCTAGCCCG
GATCGTCGGCGGGCTTCTCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSAVAGQASAAAPGRKPGAGQARGSKAASGKAPGGHNPWLIAGVVALATFMEVLDTTIANVALRYISGGLAVGPDEAAWV
VTSYLVANAITLTASSFLAKRYGRKAFFMWSVALFTASSILCGFAWSLESLLLFRVMQGLGGGGMAPLAQSILADSFPPA
KRGQAFALYGVAIVVAPVIGPTLGGWLSDNASWHWCFLINGPVGVIALGLMYWLIEDSDAAKKERRALVRKGIRFDIVGF
VLVALFLGSLEVILDEGQRKDWFGTSTLMNVSGVLMVVSFIAMIPWLLTRENPVVDLRLIGNRQFGSCFLVMMFTGAILI
ATTQFIPQITQEYYGYTATLSGLVLGPGGIVTVVMMFLTGRISSYVQPKWLIATGMTIVALGMYDLTRIDGSTTFAFFAW
SRVYIGIGLPMVFLSITSASYEGIRKDQTDQASALINVARNVGGSMGVSLAQNILAYRSQFHQSRLVESVNPASPAYQET
MRQATQYFSEHGYAGVEAQNQATAWIGSVLSTQVAYWAYIDVFWVLGLVAACAVPLALSLKSVKLGGGAPAGGH

Sequences:

>Translated_554_residues
MSAVAGQASAAAPGRKPGAGQARGSKAASGKAPGGHNPWLIAGVVALATFMEVLDTTIANVALRYISGGLAVGPDEAAWV
VTSYLVANAITLTASSFLAKRYGRKAFFMWSVALFTASSILCGFAWSLESLLLFRVMQGLGGGGMAPLAQSILADSFPPA
KRGQAFALYGVAIVVAPVIGPTLGGWLSDNASWHWCFLINGPVGVIALGLMYWLIEDSDAAKKERRALVRKGIRFDIVGF
VLVALFLGSLEVILDEGQRKDWFGTSTLMNVSGVLMVVSFIAMIPWLLTRENPVVDLRLIGNRQFGSCFLVMMFTGAILI
ATTQFIPQITQEYYGYTATLSGLVLGPGGIVTVVMMFLTGRISSYVQPKWLIATGMTIVALGMYDLTRIDGSTTFAFFAW
SRVYIGIGLPMVFLSITSASYEGIRKDQTDQASALINVARNVGGSMGVSLAQNILAYRSQFHQSRLVESVNPASPAYQET
MRQATQYFSEHGYAGVEAQNQATAWIGSVLSTQVAYWAYIDVFWVLGLVAACAVPLALSLKSVKLGGGAPAGGH
>Mature_553_residues
SAVAGQASAAAPGRKPGAGQARGSKAASGKAPGGHNPWLIAGVVALATFMEVLDTTIANVALRYISGGLAVGPDEAAWVV
TSYLVANAITLTASSFLAKRYGRKAFFMWSVALFTASSILCGFAWSLESLLLFRVMQGLGGGGMAPLAQSILADSFPPAK
RGQAFALYGVAIVVAPVIGPTLGGWLSDNASWHWCFLINGPVGVIALGLMYWLIEDSDAAKKERRALVRKGIRFDIVGFV
LVALFLGSLEVILDEGQRKDWFGTSTLMNVSGVLMVVSFIAMIPWLLTRENPVVDLRLIGNRQFGSCFLVMMFTGAILIA
TTQFIPQITQEYYGYTATLSGLVLGPGGIVTVVMMFLTGRISSYVQPKWLIATGMTIVALGMYDLTRIDGSTTFAFFAWS
RVYIGIGLPMVFLSITSASYEGIRKDQTDQASALINVARNVGGSMGVSLAQNILAYRSQFHQSRLVESVNPASPAYQETM
RQATQYFSEHGYAGVEAQNQATAWIGSVLSTQVAYWAYIDVFWVLGLVAACAVPLALSLKSVKLGGGAPAGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=487, Percent_Identity=35.7289527720739, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1788710, Length=487, Percent_Identity=33.264887063655, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI1790195, Length=438, Percent_Identity=25.1141552511416, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87081983, Length=416, Percent_Identity=31.0096153846154, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788392, Length=212, Percent_Identity=36.3207547169811, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87081723, Length=189, Percent_Identity=29.6296296296296, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=558, Percent_Identity=22.7598566308244, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=159, Percent_Identity=28.9308176100629, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=30.0751879699248, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=336, Percent_Identity=24.1071428571429, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58988; Mature: 58857

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVAGQASAAAPGRKPGAGQARGSKAASGKAPGGHNPWLIAGVVALATFMEVLDTTIAN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VALRYISGGLAVGPDEAAWVVTSYLVANAITLTASSFLAKRYGRKAFFMWSVALFTASSI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LCGFAWSLESLLLFRVMQGLGGGGMAPLAQSILADSFPPAKRGQAFALYGVAIVVAPVIG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHC
PTLGGWLSDNASWHWCFLINGPVGVIALGLMYWLIEDSDAAKKERRALVRKGIRFDIVGF
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
VLVALFLGSLEVILDEGQRKDWFGTSTLMNVSGVLMVVSFIAMIPWLLTRENPVVDLRLI
HHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
GNRQFGSCFLVMMFTGAILIATTQFIPQITQEYYGYTATLSGLVLGPGGIVTVVMMFLTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEECCCHHHHHHHHHHHH
RISSYVQPKWLIATGMTIVALGMYDLTRIDGSTTFAFFAWSRVYIGIGLPMVFLSITSAS
HHHHHCCCCEEHHCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHH
YEGIRKDQTDQASALINVARNVGGSMGVSLAQNILAYRSQFHQSRLVESVNPASPAYQET
HCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MRQATQYFSEHGYAGVEAQNQATAWIGSVLSTQVAYWAYIDVFWVLGLVAACAVPLALSL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSVKLGGGAPAGGH
HHEEECCCCCCCCC
>Mature Secondary Structure 
SAVAGQASAAAPGRKPGAGQARGSKAASGKAPGGHNPWLIAGVVALATFMEVLDTTIAN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VALRYISGGLAVGPDEAAWVVTSYLVANAITLTASSFLAKRYGRKAFFMWSVALFTASSI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LCGFAWSLESLLLFRVMQGLGGGGMAPLAQSILADSFPPAKRGQAFALYGVAIVVAPVIG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHC
PTLGGWLSDNASWHWCFLINGPVGVIALGLMYWLIEDSDAAKKERRALVRKGIRFDIVGF
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
VLVALFLGSLEVILDEGQRKDWFGTSTLMNVSGVLMVVSFIAMIPWLLTRENPVVDLRLI
HHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
GNRQFGSCFLVMMFTGAILIATTQFIPQITQEYYGYTATLSGLVLGPGGIVTVVMMFLTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEECCCHHHHHHHHHHHH
RISSYVQPKWLIATGMTIVALGMYDLTRIDGSTTFAFFAWSRVYIGIGLPMVFLSITSAS
HHHHHCCCCEEHHCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHH
YEGIRKDQTDQASALINVARNVGGSMGVSLAQNILAYRSQFHQSRLVESVNPASPAYQET
HCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MRQATQYFSEHGYAGVEAQNQATAWIGSVLSTQVAYWAYIDVFWVLGLVAACAVPLALSL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSVKLGGGAPAGGH
HHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]