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The map label for this gene is emrA [H]

Identifier: 170750012

GI number: 170750012

Start: 3839414

End: 3840682

Strand: Direct

Name: emrA [H]

Synonym: Mrad2831_3613

Alternate gene names: 170750012

Gene position: 3839414-3840682 (Clockwise)

Preceding gene: 170750009

Following gene: 170750013

Centisome position: 63.17

GC content: 71.63

Gene sequence:

>1269_bases
ATGTTTGACGATCAGCGGCACCTCTCCGAGGACGAGGCGCGCCCGCGGCCGGCCGACGGCCCGGCCGTCGCGCGCGAACG
CGACGTGCTCGATCTCGGCCGCACCGAGCCGGCCGCCGATCGCGGGGCCGACCGCGAGGCCGCGCGCAAGCCCGGCACGG
AGGGGGCCGACGACGCGCCCGAGGCGCAGGACGGGCAGGCGGGAGACGACGCCGAGGATGACGACGACGGCGCGCGGCGC
CCCAGCGTCCTGCGGCGCCACCCCGTCGCGTTCCTGCTCGGCGGCCTCGCGGTCGTCGCGCTCTGCGTCGGGGTGTTCTT
CTACTGGCTGCTCTACATGCATCCCTACGAGAGCACCGACGACGCCTTCGTCGACGCGCGCAGCATCTCGATCCAGCCGA
AGGTCGGCGGCTACCTGGTGGACGTGCCGGTCACCGACAACCAGCACGTCGAGGCCGGCCAGATCCTGTTCCAGGTCTTC
CAGCGCGACTACGACATCGCCCTCGACCAGGCGAAGGCGCAGGTCGCCGCCGACGAGGCCGGCATCAAGAACATCGACGC
GCAGATCCAGGCGCAGTACGCCAACATCGACGTGTCCAAGGCGCAGGTCGCCACCGCCGAGGCGGCGCTGAAATTCGCCC
AGGAGGACGCGGCCCGCTACAAGGACCTCGCCGAGCGCGGCTCCGGCTCGGTCCAGCAGTCGCAGTCGGCGACCTCGACG
CTGCAGCAGCGGCAGGCGGCCGTGCAGAGCGCCAATGCCAGCGTGGTCGCCGCGCAGAAGCAGGTCGGCTCGCTCCAGGC
CCAGAAGGCCTCGACCCAGGCGCAGCTCGGCCGCGACCGGGCGCAGGTCGAGCAGGCCGAGCTGAACCTCGGCTACACGA
CGGTGCGCGCCGTGCAGGGCGGCCGCATCGTGCGGCTCACCGGCGGCGTCGGCCAGCTCGCGCAGGCCGGCCAGACCCTG
GCGATGTTCGTGCCCGACGACATCTGGGTGACGGCGAACTTCAAGGAGACGCAGATCACCGACATGCGGCAGGGCCAGCC
GGTCGACATCGAGATCGACGCCTATCCCGGCCGCAAGATCCAGGGCACCGTGGCCTCGGTGCAGCCCGGCTCGGGCACCG
CCTTCAGCCTGCTGCCGGCGCAGAACGCCACCGGCAACTACGTGAAGGTGACCCAGCGCATCCCGGTCAAGATCGTGGTC
AAGAACTGGCCGACGGACGTGTCGATCGGCCCGGGCATGTCGATCGTCCCGACCGTCACGGTGCGGTGA

Upstream 100 bases:

>100_bases
GTGGAACCTTGAGCCTGCGGCCGGCTTTTGCTGCCTGAGGCGGGCGATGCGACAAGCCCGGTCACCCCCGGGTGGCCGGC
CCATCGGGCGACGACTTCCC

Downstream 100 bases:

>100_bases
GCGCCGTGGCCGGCCAGGCGAGCGCGGCCGCGCCCGGACGCAAGCCGGGTGCGGGGCAGGCGAGAGGAAGCAAGGCCGCG
TCCGGCAAGGCCCCGGGCGG

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MFDDQRHLSEDEARPRPADGPAVARERDVLDLGRTEPAADRGADREAARKPGTEGADDAPEAQDGQAGDDAEDDDDGARR
PSVLRRHPVAFLLGGLAVVALCVGVFFYWLLYMHPYESTDDAFVDARSISIQPKVGGYLVDVPVTDNQHVEAGQILFQVF
QRDYDIALDQAKAQVAADEAGIKNIDAQIQAQYANIDVSKAQVATAEAALKFAQEDAARYKDLAERGSGSVQQSQSATST
LQQRQAAVQSANASVVAAQKQVGSLQAQKASTQAQLGRDRAQVEQAELNLGYTTVRAVQGGRIVRLTGGVGQLAQAGQTL
AMFVPDDIWVTANFKETQITDMRQGQPVDIEIDAYPGRKIQGTVASVQPGSGTAFSLLPAQNATGNYVKVTQRIPVKIVV
KNWPTDVSIGPGMSIVPTVTVR

Sequences:

>Translated_422_residues
MFDDQRHLSEDEARPRPADGPAVARERDVLDLGRTEPAADRGADREAARKPGTEGADDAPEAQDGQAGDDAEDDDDGARR
PSVLRRHPVAFLLGGLAVVALCVGVFFYWLLYMHPYESTDDAFVDARSISIQPKVGGYLVDVPVTDNQHVEAGQILFQVF
QRDYDIALDQAKAQVAADEAGIKNIDAQIQAQYANIDVSKAQVATAEAALKFAQEDAARYKDLAERGSGSVQQSQSATST
LQQRQAAVQSANASVVAAQKQVGSLQAQKASTQAQLGRDRAQVEQAELNLGYTTVRAVQGGRIVRLTGGVGQLAQAGQTL
AMFVPDDIWVTANFKETQITDMRQGQPVDIEIDAYPGRKIQGTVASVQPGSGTAFSLLPAQNATGNYVKVTQRIPVKIVV
KNWPTDVSIGPGMSIVPTVTVR
>Mature_422_residues
MFDDQRHLSEDEARPRPADGPAVARERDVLDLGRTEPAADRGADREAARKPGTEGADDAPEAQDGQAGDDAEDDDDGARR
PSVLRRHPVAFLLGGLAVVALCVGVFFYWLLYMHPYESTDDAFVDARSISIQPKVGGYLVDVPVTDNQHVEAGQILFQVF
QRDYDIALDQAKAQVAADEAGIKNIDAQIQAQYANIDVSKAQVATAEAALKFAQEDAARYKDLAERGSGSVQQSQSATST
LQQRQAAVQSANASVVAAQKQVGSLQAQKASTQAQLGRDRAQVEQAELNLGYTTVRAVQGGRIVRLTGGVGQLAQAGQTL
AMFVPDDIWVTANFKETQITDMRQGQPVDIEIDAYPGRKIQGTVASVQPGSGTAFSLLPAQNATGNYVKVTQRIPVKIVV
KNWPTDVSIGPGMSIVPTVTVR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=332, Percent_Identity=33.7349397590361, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789041, Length=311, Percent_Identity=33.7620578778135, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790519, Length=307, Percent_Identity=32.5732899022801, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081951, Length=330, Percent_Identity=26.969696969697, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790024, Length=282, Percent_Identity=25.177304964539, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790012, Length=283, Percent_Identity=24.7349823321555, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45223; Mature: 45223

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDDQRHLSEDEARPRPADGPAVARERDVLDLGRTEPAADRGADREAARKPGTEGADDAP
CCCCHHCCCCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCC
EAQDGQAGDDAEDDDDGARRPSVLRRHPVAFLLGGLAVVALCVGVFFYWLLYMHPYESTD
CCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DAFVDARSISIQPKVGGYLVDVPVTDNQHVEAGQILFQVFQRDYDIALDQAKAQVAADEA
CCEEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GIKNIDAQIQAQYANIDVSKAQVATAEAALKFAQEDAARYKDLAERGSGSVQQSQSATST
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LQQRQAAVQSANASVVAAQKQVGSLQAQKASTQAQLGRDRAQVEQAELNLGYTTVRAVQG
HHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEECC
GRIVRLTGGVGQLAQAGQTLAMFVPDDIWVTANFKETQITDMRQGQPVDIEIDAYPGRKI
CEEEEEECCHHHHHHCCCEEEEEECCCEEEEECCCCCCHHHCCCCCCEEEEEECCCCCEE
QGTVASVQPGSGTAFSLLPAQNATGNYVKVTQRIPVKIVVKNWPTDVSIGPGMSIVPTVT
EEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCEECCEEE
VR
EC
>Mature Secondary Structure
MFDDQRHLSEDEARPRPADGPAVARERDVLDLGRTEPAADRGADREAARKPGTEGADDAP
CCCCHHCCCCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCC
EAQDGQAGDDAEDDDDGARRPSVLRRHPVAFLLGGLAVVALCVGVFFYWLLYMHPYESTD
CCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DAFVDARSISIQPKVGGYLVDVPVTDNQHVEAGQILFQVFQRDYDIALDQAKAQVAADEA
CCEEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GIKNIDAQIQAQYANIDVSKAQVATAEAALKFAQEDAARYKDLAERGSGSVQQSQSATST
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LQQRQAAVQSANASVVAAQKQVGSLQAQKASTQAQLGRDRAQVEQAELNLGYTTVRAVQG
HHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEECC
GRIVRLTGGVGQLAQAGQTLAMFVPDDIWVTANFKETQITDMRQGQPVDIEIDAYPGRKI
CEEEEEECCHHHHHHCCCEEEEEECCCEEEEECCCCCCHHHCCCCCCEEEEEECCCCCEE
QGTVASVQPGSGTAFSLLPAQNATGNYVKVTQRIPVKIVVKNWPTDVSIGPGMSIVPTVT
EEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCEECCEEE
VR
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]