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The map label for this gene is nth [H]

Identifier: 170749886

GI number: 170749886

Start: 3703603

End: 3704466

Strand: Direct

Name: nth [H]

Synonym: Mrad2831_3486

Alternate gene names: 170749886

Gene position: 3703603-3704466 (Clockwise)

Preceding gene: 170749881

Following gene: 170749887

Centisome position: 60.94

GC content: 71.41

Gene sequence:

>864_bases
ATGCCCGCCAGAAAGACTCCCCCTCCCGTGACGGATCGCGCCGGTCGGCGCGCCGCGGGACCGGCGGAGGCCGCGCCCGG
GGCCGTGACCGAGACGTCGCGAACGAAGCGGCGCGAGGACGCGGCGGAGAGCGTGGCCAGAGGCCCGTCCCGGAGCGCCG
CCCCGGCGCGGCTCGCGGGTCCGATCGGGCGCGCCGTCACCGCGCAGGTGGACACGGCTCCCGAGGCCGTCGACCCGGCG
ACGCTCGCCGAGATCTTCCGCCGCTTCCAGGCGGCGGAACCGGAGCCCAAGGGTGAGCTCCACTACGTGAATCCGTTCAC
GCTGCTCGTTGCCGTGGTGCTGTCGGCCCAGGCGACCGACCGCGGCGTCAATCTCGCCACCGGCCCGCTCTTCGCGGTCG
CCGACACGCCCGAGAAGATGCTGGCCCTCGGCGAGGACAGGGTCCGCGATTTCGTGCGCACGATCGGGCTGTTCAACACC
AAGGCGAAGAACGTCGTCGCCCTCTCGCGGATCCTCGTGGACGAGCACGGCGGCACGGTGCCGGCGAGCCTGGAAGCCCT
CCAGGTTCTGCCCGGTGTCGGCGCCAAGACCGCGAGCGTGGTGCTCAACATCGCCTTCGGGGTTCCGCGGATCGCCGTGG
ACACCCATATCTTCCGGGTCTCGAACCGGATCCCGCTCTTCGTCGGCGCGACCACCGACAAGGTGCAGGCCGGTCTCGAG
GCTATCGTGCCGGATTCCTACCGGCTCCACGCCCATCACTGGCTGATCCTGCACGGCCGCTACACCTGCAAGGCGCGCAA
GCCCGAATGCCCGCGCTGCCACATCGCCGACCTCTGCCGCTACCCGTCCAAGACGACCGCGTGA

Upstream 100 bases:

>100_bases
ATCGTCGCGGTGAAGACGGGCCGGTCGATCGTGGCCGGATCGAGCCCGTAGGGGTGGACAGAAGGATTGCCGCTCGCCAT
GCGATCCATATAACGCGCCG

Downstream 100 bases:

>100_bases
CGGCGGCGTGACGGGCTGCCGAGGTCGTTCCGGGCACGTCCCGGCCGGCCTCCGCAATCCCGCCCTGCGGGCTAACGCGT
TGTGCTGGAAGCCCGCATCC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MPARKTPPPVTDRAGRRAAGPAEAAPGAVTETSRTKRREDAAESVARGPSRSAAPARLAGPIGRAVTAQVDTAPEAVDPA
TLAEIFRRFQAAEPEPKGELHYVNPFTLLVAVVLSAQATDRGVNLATGPLFAVADTPEKMLALGEDRVRDFVRTIGLFNT
KAKNVVALSRILVDEHGGTVPASLEALQVLPGVGAKTASVVLNIAFGVPRIAVDTHIFRVSNRIPLFVGATTDKVQAGLE
AIVPDSYRLHAHHWLILHGRYTCKARKPECPRCHIADLCRYPSKTTA

Sequences:

>Translated_287_residues
MPARKTPPPVTDRAGRRAAGPAEAAPGAVTETSRTKRREDAAESVARGPSRSAAPARLAGPIGRAVTAQVDTAPEAVDPA
TLAEIFRRFQAAEPEPKGELHYVNPFTLLVAVVLSAQATDRGVNLATGPLFAVADTPEKMLALGEDRVRDFVRTIGLFNT
KAKNVVALSRILVDEHGGTVPASLEALQVLPGVGAKTASVVLNIAFGVPRIAVDTHIFRVSNRIPLFVGATTDKVQAGLE
AIVPDSYRLHAHHWLILHGRYTCKARKPECPRCHIADLCRYPSKTTA
>Mature_286_residues
PARKTPPPVTDRAGRRAAGPAEAAPGAVTETSRTKRREDAAESVARGPSRSAAPARLAGPIGRAVTAQVDTAPEAVDPAT
LAEIFRRFQAAEPEPKGELHYVNPFTLLVAVVLSAQATDRGVNLATGPLFAVADTPEKMLALGEDRVRDFVRTIGLFNTK
AKNVVALSRILVDEHGGTVPASLEALQVLPGVGAKTASVVLNIAFGVPRIAVDTHIFRVSNRIPLFVGATTDKVQAGLEA
IVPDSYRLHAHHWLILHGRYTCKARKPECPRCHIADLCRYPSKTTA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=180, Percent_Identity=31.6666666666667, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=52.4038461538462, Blast_Score=230, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17554540, Length=186, Percent_Identity=34.9462365591398, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=29.3785310734463, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 30601; Mature: 30470

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARKTPPPVTDRAGRRAAGPAEAAPGAVTETSRTKRREDAAESVARGPSRSAAPARLAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHC
PIGRAVTAQVDTAPEAVDPATLAEIFRRFQAAEPEPKGELHYVNPFTLLVAVVLSAQATD
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHCCC
RGVNLATGPLFAVADTPEKMLALGEDRVRDFVRTIGLFNTKAKNVVALSRILVDEHGGTV
CCCEEECCCEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
PASLEALQVLPGVGAKTASVVLNIAFGVPRIAVDTHIFRVSNRIPLFVGATTDKVQAGLE
CHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEHHHEEEEECCCCEEEEECCHHHHHHHHH
AIVPDSYRLHAHHWLILHGRYTCKARKPECPRCHIADLCRYPSKTTA
HHCCCCEEEEEEEEEEEECCEEECCCCCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
PARKTPPPVTDRAGRRAAGPAEAAPGAVTETSRTKRREDAAESVARGPSRSAAPARLAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHC
PIGRAVTAQVDTAPEAVDPATLAEIFRRFQAAEPEPKGELHYVNPFTLLVAVVLSAQATD
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHCCC
RGVNLATGPLFAVADTPEKMLALGEDRVRDFVRTIGLFNTKAKNVVALSRILVDEHGGTV
CCCEEECCCEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
PASLEALQVLPGVGAKTASVVLNIAFGVPRIAVDTHIFRVSNRIPLFVGATTDKVQAGLE
CHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEHHHEEEEECCCCEEEEECCHHHHHHHHH
AIVPDSYRLHAHHWLILHGRYTCKARKPECPRCHIADLCRYPSKTTA
HHCCCCEEEEEEEEEEEECCEEECCCCCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]