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The map label for this gene is rsuA [H]

Identifier: 170749852

GI number: 170749852

Start: 3667159

End: 3667899

Strand: Reverse

Name: rsuA [H]

Synonym: Mrad2831_3452

Alternate gene names: 170749852

Gene position: 3667899-3667159 (Counterclockwise)

Preceding gene: 170749853

Following gene: 170749850

Centisome position: 60.35

GC content: 75.44

Gene sequence:

>741_bases
ATGAGCGCGGCGAGATCCGTCCGCCTGGACAAGCTCCTCGCCAATCTCGGCTACGGCTCGCGCCGCGAGATCCAGGCCCT
CGCCCGCGCCGGCGCGATCCGCCTCGACGGGGCTGAACTCGCGGATGCCGGCGACCGGGTCGCCCTCGCGCCGGACCTGC
CGGAGCGCCTCACCGTCGACGGCGAATCCCTCGACCCGCTCCCCGGCCTGTGCCTGATGCTGCACAAGCCGCTGGGCGTG
ACCTGCTCGCACAAGGAGGCCGGCCCGCTGGTCTACGGGCTCCTCCCCGAGCGCTGGCGCCGCCGCGACCCGCCGCTCTC
CACGGTGGGACGCCTCGACAAGGAGACCTCGGGCCTGCTGCTGCTGACCGACGACGGGGCGCTGCTGCACCGGATCATCA
GCCCGAAGGCGCAGGTGGCGAAGCGCTACCGCGTGACCCTCGACCGGCCGCTGACCGGCCGCGAGGCGGAGGTGTTCGCC
GCGGGCACGCTCGTCCTGGAGGGCGAGGAGCGCCCGCTCCTGCCGGTGCGGCTCGACGTCGAGGACGCGACCCGCTGCGC
GGTGACCCTGACGGAGGGCCGGTACCACCAGGTCCGCCGGATGTTCGCGGCCGTCGGCAACCACGTCGCGGCGCTGCACC
GGGACCGGGTCGGCGGCCTCGCTCTGCCGGCGGACCTGCCGGCGGGCGCGCACCGGGTGATGACGGCCGAGGACGTGGCG
GCGGTGTTCGCGGGCGGGTGA

Upstream 100 bases:

>100_bases
TACGAGGCGAGCCTGCGCGAGGCGTTCCGGCACGTCGCGGTGGCCGTCCAGGCGAACGGCTACCGGGTCTACGAGGCCCG
CCGGTGAGGGCGCCGCTTCC

Downstream 100 bases:

>100_bases
TCCGGGCTGCGCGCCTGTCAGTGCAGAGCCCGGGCGAGGGCGACGACCGCGCCGATGACAGCGAGTGTCTGGAAGCCGAT
CGAGCCCGCCACCCAGCGCA

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSAARSVRLDKLLANLGYGSRREIQALARAGAIRLDGAELADAGDRVALAPDLPERLTVDGESLDPLPGLCLMLHKPLGV
TCSHKEAGPLVYGLLPERWRRRDPPLSTVGRLDKETSGLLLLTDDGALLHRIISPKAQVAKRYRVTLDRPLTGREAEVFA
AGTLVLEGEERPLLPVRLDVEDATRCAVTLTEGRYHQVRRMFAAVGNHVAALHRDRVGGLALPADLPAGAHRVMTAEDVA
AVFAGG

Sequences:

>Translated_246_residues
MSAARSVRLDKLLANLGYGSRREIQALARAGAIRLDGAELADAGDRVALAPDLPERLTVDGESLDPLPGLCLMLHKPLGV
TCSHKEAGPLVYGLLPERWRRRDPPLSTVGRLDKETSGLLLLTDDGALLHRIISPKAQVAKRYRVTLDRPLTGREAEVFA
AGTLVLEGEERPLLPVRLDVEDATRCAVTLTEGRYHQVRRMFAAVGNHVAALHRDRVGGLALPADLPAGAHRVMTAEDVA
AVFAGG
>Mature_245_residues
SAARSVRLDKLLANLGYGSRREIQALARAGAIRLDGAELADAGDRVALAPDLPERLTVDGESLDPLPGLCLMLHKPLGVT
CSHKEAGPLVYGLLPERWRRRDPPLSTVGRLDKETSGLLLLTDDGALLHRIISPKAQVAKRYRVTLDRPLTGREAEVFAA
GTLVLEGEERPLLPVRLDVEDATRCAVTLTEGRYHQVRRMFAAVGNHVAALHRDRVGGLALPADLPAGAHRVMTAEDVAA
VFAGG

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=245, Percent_Identity=41.6326530612245, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787524, Length=238, Percent_Identity=34.453781512605, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=28.6307053941909, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26458; Mature: 26327

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARSVRLDKLLANLGYGSRREIQALARAGAIRLDGAELADAGDRVALAPDLPERLTVD
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCHHCCCCCEEEECCCCCCCEEEC
GESLDPLPGLCLMLHKPLGVTCSHKEAGPLVYGLLPERWRRRDPPLSTVGRLDKETSGLL
CCCCCCCCHHHHHHCCCCCCEECCCCCCCEEEECCCHHHHCCCCCHHHHHCCCCCCCCEE
LLTDDGALLHRIISPKAQVAKRYRVTLDRPLTGREAEVFAAGTLVLEGEERPLLPVRLDV
EEECCCHHHHHHHCCHHHHHHHHEEEECCCCCCCCCEEEEEEEEEECCCCCCCEEEEECC
EDATRCAVTLTEGRYHQVRRMFAAVGNHVAALHRDRVGGLALPADLPAGAHRVMTAEDVA
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEHHHHH
AVFAGG
HHCCCC
>Mature Secondary Structure 
SAARSVRLDKLLANLGYGSRREIQALARAGAIRLDGAELADAGDRVALAPDLPERLTVD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCHHCCCCCEEEECCCCCCCEEEC
GESLDPLPGLCLMLHKPLGVTCSHKEAGPLVYGLLPERWRRRDPPLSTVGRLDKETSGLL
CCCCCCCCHHHHHHCCCCCCEECCCCCCCEEEECCCHHHHCCCCCHHHHHCCCCCCCCEE
LLTDDGALLHRIISPKAQVAKRYRVTLDRPLTGREAEVFAAGTLVLEGEERPLLPVRLDV
EEECCCHHHHHHHCCHHHHHHHHEEEECCCCCCCCCEEEEEEEEEECCCCCCCEEEEECC
EDATRCAVTLTEGRYHQVRRMFAAVGNHVAALHRDRVGGLALPADLPAGAHRVMTAEDVA
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEHHHHH
AVFAGG
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]