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The map label for this gene is emrA [H]

Identifier: 170749842

GI number: 170749842

Start: 3658436

End: 3659701

Strand: Reverse

Name: emrA [H]

Synonym: Mrad2831_3442

Alternate gene names: 170749842

Gene position: 3659701-3658436 (Counterclockwise)

Preceding gene: 170749844

Following gene: 170749841

Centisome position: 60.21

GC content: 73.93

Gene sequence:

>1266_bases
ATGTCCCTTCGCGAGGACGCCGATCGGCCGAGTTCCGGTCTCGAGACGGGTATTGAGACCGGTATTGAGACCCGAATCGA
GGCCGGGGACGAGGCGCGCGCCGAGCGCACCGCGCCGGCCGCCGCCGCGGCGTCCCTGCCGACGCCCGTGCCGGTCGAGG
CGCCGCCCGCGCGCAAGCGCTCCATGAGGCGGCTCGTCCTGCTCGGACTGCTGACGGCCGCCCTGGGCGGCGGCGCGTAT
CAGGGGTGGCAGTGGTGGACCGTCGGGCGGTTCTTCGTCTCGACCGACGACGCCTACGTCCAGGCCGACATCTCGGTCCT
CGCGGCCAAGATCCCGGGCTATCTCCAGTCGGTCCCGGTGGTGAACGGCCTCGCGGTGAGGAAGGGCGACGTCATCGCCA
AGCTCGACGACGGCGACTACCGCCTCGCGCTGCAGGCGGCGCAGGACAAGCTCGCCACCCAGGAGAGCACGATCGCGCGG
ATCGGCCGGCAGGCCGAGGCGGCGCAGGCCCAGGTCGCCCAGGCCGCCGCGCAGCTCGACGCGACCCGCGCCGACGCCGT
CCGCGCCCAGGCCGACTTCGCCCGGTCGACGCAGATGCAGGCGGATTACGTGGCCAAGTCGCGCATCGACCAGACCCGCG
CCGACCGCGACCGCACCGAGGCCTCCGTGAAGGCCATGGAGGCGGGCCTCCTCGCGGCCCGCGCCAACGTGGACGTGCTC
CAGGCGCAGAAGCGCGAGGCGGAGAGCCTCGCGGCGGAGCTGCGCACCGCCGTCGACCGGGCCCGGCGCGACCTCGACTT
CACGGTCATCCGCGCGCCCTTCGACGGTGTGGTCGGCAACAAGGCCGTCGAGGCCGGCGCCTATGTCGGGCCCGGCACGC
GGGTCGCCGCGCTGGTCCCGCTCCAGAGCGTCCGGATCGACGCGAACTTCAAGGAGACCCAGGTCGAGCGCATGCGTCCC
GGACAGCCGGTGACCGTGACGGTCGACGCCTATCCGGACCGTGAGATCCACGCCGTGGTGGAATCGTTCTCGCCGGCCTC
CGGCTCGGTCTTCAGCCTGCTGCCGCCCGACAACGCCACCGGCAACTTCACCAAGATCGTCCAGCGCCTGCCGGTCCGGG
TGCGCGTGCCCGAGGAGGTCGCCCGGGAGGGCCTGCTGCGGCCGGGCCTGTCGGCGGTGGTGCGGGTCGACACCCGCACC
GGCGCGGACCGCTCCGTCCTCGACCGCGCCTCCGCGCCGCGGCCGGTCAGCACCGCGAGCCGCTGA

Upstream 100 bases:

>100_bases
GAACCGTTCGGTCAAAATAACCTGCGGCTTCTTCGGGGGCGCGTCAAGTCGTGCCTGGTTATTGACCGAACGGTTCGGTC
ATCGATTTCGAGGCGCTGAT

Downstream 100 bases:

>100_bases
GACCGGCGATGGCCGCCAGCGCCGCCCTCGCGCCCGGCGCGCCGACCCGCGCCGCCGAGCCGCCCCTCGACCGCCGCCGC
ATGGTGGCGTTCGTCTGCAT

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSLREDADRPSSGLETGIETGIETRIEAGDEARAERTAPAAAAASLPTPVPVEAPPARKRSMRRLVLLGLLTAALGGGAY
QGWQWWTVGRFFVSTDDAYVQADISVLAAKIPGYLQSVPVVNGLAVRKGDVIAKLDDGDYRLALQAAQDKLATQESTIAR
IGRQAEAAQAQVAQAAAQLDATRADAVRAQADFARSTQMQADYVAKSRIDQTRADRDRTEASVKAMEAGLLAARANVDVL
QAQKREAESLAAELRTAVDRARRDLDFTVIRAPFDGVVGNKAVEAGAYVGPGTRVAALVPLQSVRIDANFKETQVERMRP
GQPVTVTVDAYPDREIHAVVESFSPASGSVFSLLPPDNATGNFTKIVQRLPVRVRVPEEVAREGLLRPGLSAVVRVDTRT
GADRSVLDRASAPRPVSTASR

Sequences:

>Translated_421_residues
MSLREDADRPSSGLETGIETGIETRIEAGDEARAERTAPAAAAASLPTPVPVEAPPARKRSMRRLVLLGLLTAALGGGAY
QGWQWWTVGRFFVSTDDAYVQADISVLAAKIPGYLQSVPVVNGLAVRKGDVIAKLDDGDYRLALQAAQDKLATQESTIAR
IGRQAEAAQAQVAQAAAQLDATRADAVRAQADFARSTQMQADYVAKSRIDQTRADRDRTEASVKAMEAGLLAARANVDVL
QAQKREAESLAAELRTAVDRARRDLDFTVIRAPFDGVVGNKAVEAGAYVGPGTRVAALVPLQSVRIDANFKETQVERMRP
GQPVTVTVDAYPDREIHAVVESFSPASGSVFSLLPPDNATGNFTKIVQRLPVRVRVPEEVAREGLLRPGLSAVVRVDTRT
GADRSVLDRASAPRPVSTASR
>Mature_420_residues
SLREDADRPSSGLETGIETGIETRIEAGDEARAERTAPAAAAASLPTPVPVEAPPARKRSMRRLVLLGLLTAALGGGAYQ
GWQWWTVGRFFVSTDDAYVQADISVLAAKIPGYLQSVPVVNGLAVRKGDVIAKLDDGDYRLALQAAQDKLATQESTIARI
GRQAEAAQAQVAQAAAQLDATRADAVRAQADFARSTQMQADYVAKSRIDQTRADRDRTEASVKAMEAGLLAARANVDVLQ
AQKREAESLAAELRTAVDRARRDLDFTVIRAPFDGVVGNKAVEAGAYVGPGTRVAALVPLQSVRIDANFKETQVERMRPG
QPVTVTVDAYPDREIHAVVESFSPASGSVFSLLPPDNATGNFTKIVQRLPVRVRVPEEVAREGLLRPGLSAVVRVDTRTG
ADRSVLDRASAPRPVSTASR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=384, Percent_Identity=33.8541666666667, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1788711, Length=370, Percent_Identity=31.6216216216216, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790519, Length=321, Percent_Identity=26.1682242990654, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790024, Length=239, Percent_Identity=26.7782426778243, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790012, Length=223, Percent_Identity=27.8026905829596, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789637, Length=346, Percent_Identity=24.5664739884393, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789900, Length=346, Percent_Identity=28.0346820809249, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45008; Mature: 44877

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLREDADRPSSGLETGIETGIETRIEAGDEARAERTAPAAAAASLPTPVPVEAPPARKR
CCCCCCCCCCCCHHHHHHHHCCHHHHCCCCHHHHHHCCCHHHHCCCCCCCCCCCCCHHHH
SMRRLVLLGLLTAALGGGAYQGWQWWTVGRFFVSTDDAYVQADISVLAAKIPGYLQSVPV
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCEEHHHHHHHHHHHCHHHHHCCC
VNGLAVRKGDVIAKLDDGDYRLALQAAQDKLATQESTIARIGRQAEAAQAQVAQAAAQLD
CCCEEEECCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ATRADAVRAQADFARSTQMQADYVAKSRIDQTRADRDRTEASVKAMEAGLLAARANVDVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QAQKREAESLAAELRTAVDRARRDLDFTVIRAPFDGVVGNKAVEAGAYVGPGTRVAALVP
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHCCCEECCCCCEEEEEC
LQSVRIDANFKETQVERMRPGQPVTVTVDAYPDREIHAVVESFSPASGSVFSLLPPDNAT
HHHEEECCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCCC
GNFTKIVQRLPVRVRVPEEVAREGLLRPGLSAVVRVDTRTGADRSVLDRASAPRPVSTAS
CHHHHHHHHCCCEEECCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCC
R
C
>Mature Secondary Structure 
SLREDADRPSSGLETGIETGIETRIEAGDEARAERTAPAAAAASLPTPVPVEAPPARKR
CCCCCCCCCCCHHHHHHHHCCHHHHCCCCHHHHHHCCCHHHHCCCCCCCCCCCCCHHHH
SMRRLVLLGLLTAALGGGAYQGWQWWTVGRFFVSTDDAYVQADISVLAAKIPGYLQSVPV
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCEEHHHHHHHHHHHCHHHHHCCC
VNGLAVRKGDVIAKLDDGDYRLALQAAQDKLATQESTIARIGRQAEAAQAQVAQAAAQLD
CCCEEEECCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ATRADAVRAQADFARSTQMQADYVAKSRIDQTRADRDRTEASVKAMEAGLLAARANVDVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QAQKREAESLAAELRTAVDRARRDLDFTVIRAPFDGVVGNKAVEAGAYVGPGTRVAALVP
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHCCCEECCCCCEEEEEC
LQSVRIDANFKETQVERMRPGQPVTVTVDAYPDREIHAVVESFSPASGSVFSLLPPDNAT
HHHEEECCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCCC
GNFTKIVQRLPVRVRVPEEVAREGLLRPGLSAVVRVDTRTGADRSVLDRASAPRPVSTAS
CHHHHHHHHCCCEEECCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]