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The map label for this gene is emrB [H]

Identifier: 170749841

GI number: 170749841

Start: 3656841

End: 3658427

Strand: Reverse

Name: emrB [H]

Synonym: Mrad2831_3441

Alternate gene names: 170749841

Gene position: 3658427-3656841 (Counterclockwise)

Preceding gene: 170749842

Following gene: 170749840

Centisome position: 60.19

GC content: 68.37

Gene sequence:

>1587_bases
ATGGCCGCCAGCGCCGCCCTCGCGCCCGGCGCGCCGACCCGCGCCGCCGAGCCGCCCCTCGACCGCCGCCGCATGGTGGC
GTTCGTCTGCATGGTCTTCGGCATGTTCATGGCGATCCTGGACATCCAGATCGTCTCGGCCTCCCTGAACGAGATCCAGG
CCGGGCTCTCGGCCTCCGGCGACGAGATCCCCTGGGTCCAGACCAGCTACCTGATTGCCGAGGTCATCTCGATCCCGCTC
TCCGGCACCCTGTCGCGGGTGCTCTCGACGCGCTGGATGTTCGCCATCTCGGCGGGCGGCTTCACCCTGATGAGCCTGAT
GTGCGCCACCTCCTCGTCGATCGGCGAGATGATCGTCTGGCGGGCGCTCCAGGGCTTCATCGGCGGCGGCATGATCCCGA
CCGTCTTCGCCGCGGCCTTCACGATCTTCCCGCCCTCGAAGCGGACCATCGTCTCGCCGATGATCGGCCTCGTGGCGACG
CTGGCGCCGACGATCGGCCCGACGGTGGGCGGCTACCTGACTGACCTGATGTCGTGGCACTGGCTGTTCCTGATCAACGT
CGTGCCGGGCATCTTCGTGACCGTCTCGACCTGGATCCTGGTCGATTTCGACGAGCCCAATTACGGCCTGATCAGATCGT
TCGACTGGGCCGGCCTCGCCTTCATGGCCGGCTTCCTCGGCTGCCTCGAGTACGTGCTGGAGGAGGGGCCGAACCACGAC
TGGCTGCAGGACGAGGCGGTCTTCGCCTGCGCGGTCGTCTGCGGGGCGTCGGCGCTGCTGTTCTTCTGGCGGGTCTTCAC
GGCGAAGCAGCCGATCGTCGATCTCCGGGCCTTCTCGGACCGGAACTTCGCCAGCGGCTGCGTGGCGAGCTTCGTGCTGG
GCATCGGCCTCTACGGTCTGACCTACATCTACCCGGTCTACCTCGCCCGGGTGCGCGGCTACTCGGCGCTGATGATCGGC
GAGACGATGTTCGTCTCCGGCCTGTGCATGTTCGCCACCGCGCCGATCGCCGGCCGGCTCTCGGGCAGGGTCGATCCCCG
GATCATGATGGGGATCGGCTTCGTCGGCTTCGCCTGCGGCACCTGGATCGTCACCGGGCTCACCAAGGACTGGGACTTCT
ACGAGCTGCTGCTGCCGCAGATCCTGCGCGGCTGCTCGCTGATGCTGTGCATGATCCCGATCAACAACATCGCGCTGGGC
ACGCTGCCGCCGGCACGGATGAAGAACGCGTCGGGGCTGTTCAACCTGACCCGCAACCTCGGCGGCGCGGTCGGGCTCGC
GCTGATCAACACGATCCTCAACGACCGCTGGGACCTGCACCTCGCCCGGCTGCACGAGCGCTTCACCTGGGCGAACACGG
CCGCGCTGGAGCGCCTCGACGCGCTGCAGCGCGGCTTCGACGCCTACGGCAGCGCCGCCTCCGGCATGGCCCTGAAGGCG
ATGACCAACACGGTGCGGATCCAGGGCCTCGTCATGGCCTTCGAGGACGTGTTCCTGTCCCTCACCGTCCTGTTCCTCGC
GATGGCCTGCGCGGTCCCCATGATCCGGCGGCCGAATGCCGGCGCGGGCGGAGGCGGCGGCCACTGA

Upstream 100 bases:

>100_bases
CGGTGGTGCGGGTCGACACCCGCACCGGCGCGGACCGCTCCGTCCTCGACCGCGCCTCCGCGCCGCGGCCGGTCAGCACC
GCGAGCCGCTGAGACCGGCG

Downstream 100 bases:

>100_bases
CGGGCGGGACCGCCGCGCGCGATCGGCCGGTGCGGTTTCGGACGGTGCGTGCGACGTTCCGCGGCCGAGCCGCAGGATTG
CGTTGGCGGTCGGCACCGCC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MAASAALAPGAPTRAAEPPLDRRRMVAFVCMVFGMFMAILDIQIVSASLNEIQAGLSASGDEIPWVQTSYLIAEVISIPL
SGTLSRVLSTRWMFAISAGGFTLMSLMCATSSSIGEMIVWRALQGFIGGGMIPTVFAAAFTIFPPSKRTIVSPMIGLVAT
LAPTIGPTVGGYLTDLMSWHWLFLINVVPGIFVTVSTWILVDFDEPNYGLIRSFDWAGLAFMAGFLGCLEYVLEEGPNHD
WLQDEAVFACAVVCGASALLFFWRVFTAKQPIVDLRAFSDRNFASGCVASFVLGIGLYGLTYIYPVYLARVRGYSALMIG
ETMFVSGLCMFATAPIAGRLSGRVDPRIMMGIGFVGFACGTWIVTGLTKDWDFYELLLPQILRGCSLMLCMIPINNIALG
TLPPARMKNASGLFNLTRNLGGAVGLALINTILNDRWDLHLARLHERFTWANTAALERLDALQRGFDAYGSAASGMALKA
MTNTVRIQGLVMAFEDVFLSLTVLFLAMACAVPMIRRPNAGAGGGGGH

Sequences:

>Translated_528_residues
MAASAALAPGAPTRAAEPPLDRRRMVAFVCMVFGMFMAILDIQIVSASLNEIQAGLSASGDEIPWVQTSYLIAEVISIPL
SGTLSRVLSTRWMFAISAGGFTLMSLMCATSSSIGEMIVWRALQGFIGGGMIPTVFAAAFTIFPPSKRTIVSPMIGLVAT
LAPTIGPTVGGYLTDLMSWHWLFLINVVPGIFVTVSTWILVDFDEPNYGLIRSFDWAGLAFMAGFLGCLEYVLEEGPNHD
WLQDEAVFACAVVCGASALLFFWRVFTAKQPIVDLRAFSDRNFASGCVASFVLGIGLYGLTYIYPVYLARVRGYSALMIG
ETMFVSGLCMFATAPIAGRLSGRVDPRIMMGIGFVGFACGTWIVTGLTKDWDFYELLLPQILRGCSLMLCMIPINNIALG
TLPPARMKNASGLFNLTRNLGGAVGLALINTILNDRWDLHLARLHERFTWANTAALERLDALQRGFDAYGSAASGMALKA
MTNTVRIQGLVMAFEDVFLSLTVLFLAMACAVPMIRRPNAGAGGGGGH
>Mature_527_residues
AASAALAPGAPTRAAEPPLDRRRMVAFVCMVFGMFMAILDIQIVSASLNEIQAGLSASGDEIPWVQTSYLIAEVISIPLS
GTLSRVLSTRWMFAISAGGFTLMSLMCATSSSIGEMIVWRALQGFIGGGMIPTVFAAAFTIFPPSKRTIVSPMIGLVATL
APTIGPTVGGYLTDLMSWHWLFLINVVPGIFVTVSTWILVDFDEPNYGLIRSFDWAGLAFMAGFLGCLEYVLEEGPNHDW
LQDEAVFACAVVCGASALLFFWRVFTAKQPIVDLRAFSDRNFASGCVASFVLGIGLYGLTYIYPVYLARVRGYSALMIGE
TMFVSGLCMFATAPIAGRLSGRVDPRIMMGIGFVGFACGTWIVTGLTKDWDFYELLLPQILRGCSLMLCMIPINNIALGT
LPPARMKNASGLFNLTRNLGGAVGLALINTILNDRWDLHLARLHERFTWANTAALERLDALQRGFDAYGSAASGMALKAM
TNTVRIQGLVMAFEDVFLSLTVLFLAMACAVPMIRRPNAGAGGGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=505, Percent_Identity=29.9009900990099, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=28.8577154308617, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1790195, Length=458, Percent_Identity=24.4541484716157, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788392, Length=403, Percent_Identity=27.0471464019851, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081983, Length=422, Percent_Identity=26.5402843601896, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325455, Length=152, Percent_Identity=30.2631578947368, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56874; Mature: 56743

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASAALAPGAPTRAAEPPLDRRRMVAFVCMVFGMFMAILDIQIVSASLNEIQAGLSASG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEIPWVQTSYLIAEVISIPLSGTLSRVLSTRWMFAISAGGFTLMSLMCATSSSIGEMIVW
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCHHHHHHHH
RALQGFIGGGMIPTVFAAAFTIFPPSKRTIVSPMIGLVATLAPTIGPTVGGYLTDLMSWH
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WLFLINVVPGIFVTVSTWILVDFDEPNYGLIRSFDWAGLAFMAGFLGCLEYVLEEGPNHD
HHHHHHHHHHHHHEEEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
WLQDEAVFACAVVCGASALLFFWRVFTAKQPIVDLRAFSDRNFASGCVASFVLGIGLYGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TYIYPVYLARVRGYSALMIGETMFVSGLCMFATAPIAGRLSGRVDPRIMMGIGFVGFACG
HHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHH
TWIVTGLTKDWDFYELLLPQILRGCSLMLCMIPINNIALGTLPPARMKNASGLFNLTRNL
HHHHHCCCCCCHHHHHHHHHHHHHHHHEEHEEECCCEEECCCCHHHHCCCCHHHHHHHHH
GGAVGLALINTILNDRWDLHLARLHERFTWANTAALERLDALQRGFDAYGSAASGMALKA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHH
MTNTVRIQGLVMAFEDVFLSLTVLFLAMACAVPMIRRPNAGAGGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AASAALAPGAPTRAAEPPLDRRRMVAFVCMVFGMFMAILDIQIVSASLNEIQAGLSASG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEIPWVQTSYLIAEVISIPLSGTLSRVLSTRWMFAISAGGFTLMSLMCATSSSIGEMIVW
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCHHHHHHHH
RALQGFIGGGMIPTVFAAAFTIFPPSKRTIVSPMIGLVATLAPTIGPTVGGYLTDLMSWH
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WLFLINVVPGIFVTVSTWILVDFDEPNYGLIRSFDWAGLAFMAGFLGCLEYVLEEGPNHD
HHHHHHHHHHHHHEEEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
WLQDEAVFACAVVCGASALLFFWRVFTAKQPIVDLRAFSDRNFASGCVASFVLGIGLYGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TYIYPVYLARVRGYSALMIGETMFVSGLCMFATAPIAGRLSGRVDPRIMMGIGFVGFACG
HHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHH
TWIVTGLTKDWDFYELLLPQILRGCSLMLCMIPINNIALGTLPPARMKNASGLFNLTRNL
HHHHHCCCCCCHHHHHHHHHHHHHHHHEEHEEECCCEEECCCCHHHHCCCCHHHHHHHHH
GGAVGLALINTILNDRWDLHLARLHERFTWANTAALERLDALQRGFDAYGSAASGMALKA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHH
MTNTVRIQGLVMAFEDVFLSLTVLFLAMACAVPMIRRPNAGAGGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]