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The map label for this gene is folC [H]

Identifier: 170749781

GI number: 170749781

Start: 3589049

End: 3590374

Strand: Reverse

Name: folC [H]

Synonym: Mrad2831_3380

Alternate gene names: 170749781

Gene position: 3590374-3589049 (Counterclockwise)

Preceding gene: 170749782

Following gene: 170749780

Centisome position: 59.07

GC content: 72.4

Gene sequence:

>1326_bases
ATGGCCTCGTCCGACGCCCTGATGGCGCGCTTCCTGGCGCTCCACCCCCGCACCATCGACCTGTCGCTCGGCCGCATCGA
GCGGCTGCTCGCGCGGCTGAACCATCCGGAGCGCCGCCTGCCGCCCGTGATCCACGTGGCCGGCACCAACGGCAAGGGCT
CGACCATCGCGTTCATGCGGTCGATCCTGGAGGCCGGCGGCTTGGCCGCCCACGTCTACACCTCCCCGCACCTCGTGCGA
TTCCACGAGCGGATGCGCATCGGCGGGATCGGCGGCGGGCACTTCGTGGACGAGGACCGTCTGGCCGACGCCTTCGCGCG
CTGTGAGAGCGCCAATGCCGGCGAGCCGATCACGATGTTCGAGATCACCACGGCCGCGGCGTTCCTGCTCTTCTCGGAGA
GCCCCGCCGACGTGCTGCTCCTGGAAGTCGGCCTCGGCGGCCGGCTCGACGCCACCAACGTGATCGACCGGCCGGCCTGC
GCGGTGGTGACGCCGATCGGCCGCGACCATGCCGAGTATCTCGGCGACACGGTCGAGTCGGTCGCGACCGAGAAGGCCGG
GATCTTCAAGCGCGGCTGCCCGGCGGTGATCGCCGCCCAGGACTACGCCGAGGCCGACGCCGTCCTCTGCCGGCGCGCGG
AAGCCGTCGGCGCCGGGCCCGTGCTGGTCGGTAATCAGGACTTCTCCGTGCACGAGGAGCGCGGCCGGCTCGTCTACCAG
GACGAGACCGACCTGTTCGACCTCCCGCGGCCGCGCCTCGCCGGGCGCCATCAGCTGGTCAACGCCGGAACGGCGATCAC
CGCGCTGCGCGCCGCCGGGTTCGGCGATATCGGGACGGCGGCCCTAGAGCGGGGGCTGACCGAGGTCGAGTGGCCCGGCC
GGCTGCAGCGTCTCGGGCGCGGCCGACTGGCCGCCCAGCTCGATCCCGGCACCGAGCTCTGGCTCGACGGCGGCCACAAC
ATCGACGGCGGCCGGATCCTCGCCGCCGCCATGGCGGATCTCGGCGAGAAGAGCGACGTGCCGCTCGTCCTCGTGGTCGG
CCTGCTCGGCACGAAGGACGCCGACGGCTTCCTGCGCAACTTCGTGGGTCTGGCTCGGGCCCTCGTGGCCGTTCCGATCA
CCGGAACGATGGCCGCGCGCCCGGCCGAGGAGGTCGCGCGGATCGGCGAGGAGGTCGGCCTCTCGACCTACGCGGCGCCG
AGCATCGAGGCGGCCCTTGCCCTGGTGCGGGACATCGCCTTCGAGCGCCCTCCGCGGATCTTGATCTGCGGATCGCTCTA
CTTGGCCGGCATGGTTCTCGCCGCCAACGACACGCCGCCGGTCTGA

Upstream 100 bases:

>100_bases
ACCAGCTCAAGGAGACGATCGCGCGTCTCTGCAGTCTCCTGACCAACGTGTCCCAGGCCGGCGCACCGGCCGCGGCCTCC
GAGGCGGCCTGAGCCCGGCG

Downstream 100 bases:

>100_bases
GTCGTCTCCAGTCTGGCGACTCCCCGCACGCCGGCTGTATCTAGTGGCCGGCACGCCGGACACCTGTGCAATTCGGATTC
TGCATTCGAAAATCCGTCGA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MASSDALMARFLALHPRTIDLSLGRIERLLARLNHPERRLPPVIHVAGTNGKGSTIAFMRSILEAGGLAAHVYTSPHLVR
FHERMRIGGIGGGHFVDEDRLADAFARCESANAGEPITMFEITTAAAFLLFSESPADVLLLEVGLGGRLDATNVIDRPAC
AVVTPIGRDHAEYLGDTVESVATEKAGIFKRGCPAVIAAQDYAEADAVLCRRAEAVGAGPVLVGNQDFSVHEERGRLVYQ
DETDLFDLPRPRLAGRHQLVNAGTAITALRAAGFGDIGTAALERGLTEVEWPGRLQRLGRGRLAAQLDPGTELWLDGGHN
IDGGRILAAAMADLGEKSDVPLVLVVGLLGTKDADGFLRNFVGLARALVAVPITGTMAARPAEEVARIGEEVGLSTYAAP
SIEAALALVRDIAFERPPRILICGSLYLAGMVLAANDTPPV

Sequences:

>Translated_441_residues
MASSDALMARFLALHPRTIDLSLGRIERLLARLNHPERRLPPVIHVAGTNGKGSTIAFMRSILEAGGLAAHVYTSPHLVR
FHERMRIGGIGGGHFVDEDRLADAFARCESANAGEPITMFEITTAAAFLLFSESPADVLLLEVGLGGRLDATNVIDRPAC
AVVTPIGRDHAEYLGDTVESVATEKAGIFKRGCPAVIAAQDYAEADAVLCRRAEAVGAGPVLVGNQDFSVHEERGRLVYQ
DETDLFDLPRPRLAGRHQLVNAGTAITALRAAGFGDIGTAALERGLTEVEWPGRLQRLGRGRLAAQLDPGTELWLDGGHN
IDGGRILAAAMADLGEKSDVPLVLVVGLLGTKDADGFLRNFVGLARALVAVPITGTMAARPAEEVARIGEEVGLSTYAAP
SIEAALALVRDIAFERPPRILICGSLYLAGMVLAANDTPPV
>Mature_440_residues
ASSDALMARFLALHPRTIDLSLGRIERLLARLNHPERRLPPVIHVAGTNGKGSTIAFMRSILEAGGLAAHVYTSPHLVRF
HERMRIGGIGGGHFVDEDRLADAFARCESANAGEPITMFEITTAAAFLLFSESPADVLLLEVGLGGRLDATNVIDRPACA
VVTPIGRDHAEYLGDTVESVATEKAGIFKRGCPAVIAAQDYAEADAVLCRRAEAVGAGPVLVGNQDFSVHEERGRLVYQD
ETDLFDLPRPRLAGRHQLVNAGTAITALRAAGFGDIGTAALERGLTEVEWPGRLQRLGRGRLAAQLDPGTELWLDGGHNI
DGGRILAAAMADLGEKSDVPLVLVVGLLGTKDADGFLRNFVGLARALVAVPITGTMAARPAEEVARIGEEVGLSTYAAPS
IEAALALVRDIAFERPPRILICGSLYLAGMVLAANDTPPV

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=291, Percent_Identity=34.020618556701, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI66932984, Length=291, Percent_Identity=34.020618556701, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1788654, Length=432, Percent_Identity=33.3333333333333, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71984923, Length=320, Percent_Identity=31.5625, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17553148, Length=320, Percent_Identity=31.5625, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=320, Percent_Identity=31.5625, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=440, Percent_Identity=29.0909090909091, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6324815, Length=457, Percent_Identity=25.601750547046, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24641571, Length=316, Percent_Identity=26.8987341772152, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=32.4022346368715, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46697; Mature: 46566

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSDALMARFLALHPRTIDLSLGRIERLLARLNHPERRLPPVIHVAGTNGKGSTIAFMR
CCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCHHHCCCEEEEECCCCCCHHHHHHH
SILEAGGLAAHVYTSPHLVRFHERMRIGGIGGGHFVDEDRLADAFARCESANAGEPITMF
HHHHHCCEEEEEECCCHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
EITTAAAFLLFSESPADVLLLEVGLGGRLDATNVIDRPACAVVTPIGRDHAEYLGDTVES
EHHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHH
VATEKAGIFKRGCPAVIAAQDYAEADAVLCRRAEAVGAGPVLVGNQDFSVHEERGRLVYQ
HHHHHHCHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEECCCCCCEECCCCCEEEE
DETDLFDLPRPRLAGRHQLVNAGTAITALRAAGFGDIGTAALERGLTEVEWPGRLQRLGR
CCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHCC
GRLAAQLDPGTELWLDGGHNIDGGRILAAAMADLGEKSDVPLVLVVGLLGTKDADGFLRN
CCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHCCCCHHHHHHH
FVGLARALVAVPITGTMAARPAEEVARIGEEVGLSTYAAPSIEAALALVRDIAFERPPRI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCE
LICGSLYLAGMVLAANDTPPV
EEECCHHHHCEEEECCCCCCC
>Mature Secondary Structure 
ASSDALMARFLALHPRTIDLSLGRIERLLARLNHPERRLPPVIHVAGTNGKGSTIAFMR
CCCHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCHHHCCCEEEEECCCCCCHHHHHHH
SILEAGGLAAHVYTSPHLVRFHERMRIGGIGGGHFVDEDRLADAFARCESANAGEPITMF
HHHHHCCEEEEEECCCHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
EITTAAAFLLFSESPADVLLLEVGLGGRLDATNVIDRPACAVVTPIGRDHAEYLGDTVES
EHHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHH
VATEKAGIFKRGCPAVIAAQDYAEADAVLCRRAEAVGAGPVLVGNQDFSVHEERGRLVYQ
HHHHHHCHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEECCCCCCEECCCCCEEEE
DETDLFDLPRPRLAGRHQLVNAGTAITALRAAGFGDIGTAALERGLTEVEWPGRLQRLGR
CCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHCC
GRLAAQLDPGTELWLDGGHNIDGGRILAAAMADLGEKSDVPLVLVVGLLGTKDADGFLRN
CCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHCCCCHHHHHHH
FVGLARALVAVPITGTMAARPAEEVARIGEEVGLSTYAAPSIEAALALVRDIAFERPPRI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCE
LICGSLYLAGMVLAANDTPPV
EEECCHHHHCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]