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The map label for this gene is hmp [H]

Identifier: 170749727

GI number: 170749727

Start: 3527208

End: 3529271

Strand: Direct

Name: hmp [H]

Synonym: Mrad2831_3326

Alternate gene names: 170749727

Gene position: 3527208-3529271 (Clockwise)

Preceding gene: 170749726

Following gene: 170749728

Centisome position: 58.03

GC content: 73.64

Gene sequence:

>2064_bases
ATGGCTGACGGTCAAACCTTTGCGACGCTGCCGACCTGGCATGCCGGCGAGACGTCCATCCAGGAGTCGGTCGGCGCCGC
CGACCGGATGGCGGTGATCGGCCAGAGGGTGGTGCGAGATTTCATGCCCGATCAGCACCGCGCCTTCTACGCGCAGATCC
CCTTCATCGTGCTGGGCAGCGTCGACCGGCAGGGCGACGCCTGGGCCACGCTCCTGGCCGGCAGGCCCGGCTTCATCACC
TCGCCCTCGGCCACGACCCTCGACATCGCCGCGCGACCGGACCCGGCCGACCCCGCGTCCGAGGGCACGCGCGCCGGCGA
CGCCATCGGTCTCCTGGGGATCGAGCTGAGCAGCCGCCGCCGCAACCGCGTCAACGGCCTCGTCCGCTCCACGGACGGCG
GGGTCCTGCACTTCGACGTCGATCAGAGCTTCGGGAACTGCCCGCAATACATCCAGCTGCGCGACATGACCTTCGTGGGC
GAGCCGGGCGTCCCCGTCCCGGTCTCCGCCGAGGAGAGCGCCGAGCTCGACGCGTCGGCGCGCGCCCTGATCGCGGCGGC
CGACACGTTCTTCGTGGCCTCCTACGCCGACCGCGAGGGCCGGCGCCAGGTCGACGTCTCGCATCGCGGCGGCAAGGCGG
GGTTCGTCCGCGTCGCCGCGGACGGCACGCTGACCATCCCCGACTTCGCCGGCAACCTGTTCTTCGCCACCCTCGGGAAC
ATCCTCCTGAACGGCAGGGCCGGCCTGGTCTTCACCGATTTCGAGACGGGCGATCTCCTGCAGCTGACCGGCGACGCCGC
GGTGATCCTGGACTCGCCCGAGATCGCCGCGTTCCAGGGCGCCGAGCGGCTCTGGACCTTCCGGCCGCGCCGGGTGATCC
GCCGGCGCGGCGCCCTGCCGCTGCGCTGGACGTTCCGGGCGGACGGCTGGTCGCCGAACGCGCTGATGACCGGCGACTGG
GCGGAGGCCGCCGACCGCGTCCGGGCGACCGGGCGCGCGACGCGGTGGCGGCCGTTCCGGGTGGCGAAGATCGTCGACGA
GAGCCGCACCATCCGGTCCTTCCACCTGGCACCGGACGACGGGGCCGGGCAACTCCCGCACCGCGCGGGCCAGCATCTCC
CGATCCGCGTGACGCTGCCCGGGGCGGAGGCGCCGGTGATCCGGACCTACACGCTCTCGGTCGCGCCCTCGGATCCCACC
TACCGGATCAGCGTGAAGCGCGACGGCGCCGTCTCGCAGCACCTCCACGACCGCCTTCGGGTCGGGGACATCGTCGAGGC
GCGCGCGCCCGACGGCGGCTTCACCCTCGATGCGGGCGCGGCGCGCCCCGCGGTGCTGCTCGCCGGCGGCGTCGGGATCA
CGCCGCTCCTCGCGATGCTCCGGCACGTGGTCTACGAGGGCCTGCGCACCCGCAGGATCCGCCCGACCACGCTCGTCTAC
GCCGCCCGCACGCGCGCCGAGCGCGCCTTCGAGGGGGAGCTGTCCGAACTCGCCGCGGCGGCGCAGGGGGCGGTCCGGAT
CGTGCGCGTCCTGAGCGACGTGTCCGATGCCCGGCCCGGCACCGATTACGAGGCCGCGGGCCGGATCGATATGGCGCTGC
TCACGCGGTTCCTCGCCTTCGCGGACTACGACTTCTACCTGTGCGGCCCACCGGCTTTCACCCAGGCGCTCTACGACGGG
TTGCGCGGCTACAACGTGGCGGACGACCGGATCCACGCGGAGGCGTTCGGGCCCTCGGGGCTGGTCCGCAACGCGGGGAC
GGCGGTGGCCGCGCGCCCGCGCCCGCCGGCCGCCACCGAGACGGTGCCGGTCGCCTTTCTCGACACCGGTAAGGAGGCGC
GCTGGACGCCGAATTCAGGCAGCCTCCTCGACCTCGCCGAAGCCCGCGGCCTCGCGCCGGATTTCAGCTGCCGCGAAGGC
AGCTGCGGGACCTGCCGGACCAAGCTGCTCGCCGGCGCGGTGACCTACCTCCGGGAACCGAGCGCGCCCGTGGCGGAGGA
CGAGGTGCTGATCTGCTCCGCAGTGCCCGCGGAGGGCACAGGCCGGGTGCAGCTCGCGCTCTGA

Upstream 100 bases:

>100_bases
CCCTTCGCCGAGACTCCCGCGGGCCTCACGGCGGCGGCGTGACCAGAAGAGCCCCGGGCGCACCCGCGCCCGGGAGCTGG
ACCGCGTGGAGGAGGCGGAG

Downstream 100 bases:

>100_bases
CGCGCGGGACGGCGCGGCCCGCGCCGTTTTCCACCTTCCGCGCCCGGCGGCGCCGGATCATTCTGCGCAGCGCCGATACC
GGAGCCGGGCTGAACCTTCA

Product: oxidoreductase FAD/NAD(P)-binding subunit

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MADGQTFATLPTWHAGETSIQESVGAADRMAVIGQRVVRDFMPDQHRAFYAQIPFIVLGSVDRQGDAWATLLAGRPGFIT
SPSATTLDIAARPDPADPASEGTRAGDAIGLLGIELSSRRRNRVNGLVRSTDGGVLHFDVDQSFGNCPQYIQLRDMTFVG
EPGVPVPVSAEESAELDASARALIAAADTFFVASYADREGRRQVDVSHRGGKAGFVRVAADGTLTIPDFAGNLFFATLGN
ILLNGRAGLVFTDFETGDLLQLTGDAAVILDSPEIAAFQGAERLWTFRPRRVIRRRGALPLRWTFRADGWSPNALMTGDW
AEAADRVRATGRATRWRPFRVAKIVDESRTIRSFHLAPDDGAGQLPHRAGQHLPIRVTLPGAEAPVIRTYTLSVAPSDPT
YRISVKRDGAVSQHLHDRLRVGDIVEARAPDGGFTLDAGAARPAVLLAGGVGITPLLAMLRHVVYEGLRTRRIRPTTLVY
AARTRAERAFEGELSELAAAAQGAVRIVRVLSDVSDARPGTDYEAAGRIDMALLTRFLAFADYDFYLCGPPAFTQALYDG
LRGYNVADDRIHAEAFGPSGLVRNAGTAVAARPRPPAATETVPVAFLDTGKEARWTPNSGSLLDLAEARGLAPDFSCREG
SCGTCRTKLLAGAVTYLREPSAPVAEDEVLICSAVPAEGTGRVQLAL

Sequences:

>Translated_687_residues
MADGQTFATLPTWHAGETSIQESVGAADRMAVIGQRVVRDFMPDQHRAFYAQIPFIVLGSVDRQGDAWATLLAGRPGFIT
SPSATTLDIAARPDPADPASEGTRAGDAIGLLGIELSSRRRNRVNGLVRSTDGGVLHFDVDQSFGNCPQYIQLRDMTFVG
EPGVPVPVSAEESAELDASARALIAAADTFFVASYADREGRRQVDVSHRGGKAGFVRVAADGTLTIPDFAGNLFFATLGN
ILLNGRAGLVFTDFETGDLLQLTGDAAVILDSPEIAAFQGAERLWTFRPRRVIRRRGALPLRWTFRADGWSPNALMTGDW
AEAADRVRATGRATRWRPFRVAKIVDESRTIRSFHLAPDDGAGQLPHRAGQHLPIRVTLPGAEAPVIRTYTLSVAPSDPT
YRISVKRDGAVSQHLHDRLRVGDIVEARAPDGGFTLDAGAARPAVLLAGGVGITPLLAMLRHVVYEGLRTRRIRPTTLVY
AARTRAERAFEGELSELAAAAQGAVRIVRVLSDVSDARPGTDYEAAGRIDMALLTRFLAFADYDFYLCGPPAFTQALYDG
LRGYNVADDRIHAEAFGPSGLVRNAGTAVAARPRPPAATETVPVAFLDTGKEARWTPNSGSLLDLAEARGLAPDFSCREG
SCGTCRTKLLAGAVTYLREPSAPVAEDEVLICSAVPAEGTGRVQLAL
>Mature_686_residues
ADGQTFATLPTWHAGETSIQESVGAADRMAVIGQRVVRDFMPDQHRAFYAQIPFIVLGSVDRQGDAWATLLAGRPGFITS
PSATTLDIAARPDPADPASEGTRAGDAIGLLGIELSSRRRNRVNGLVRSTDGGVLHFDVDQSFGNCPQYIQLRDMTFVGE
PGVPVPVSAEESAELDASARALIAAADTFFVASYADREGRRQVDVSHRGGKAGFVRVAADGTLTIPDFAGNLFFATLGNI
LLNGRAGLVFTDFETGDLLQLTGDAAVILDSPEIAAFQGAERLWTFRPRRVIRRRGALPLRWTFRADGWSPNALMTGDWA
EAADRVRATGRATRWRPFRVAKIVDESRTIRSFHLAPDDGAGQLPHRAGQHLPIRVTLPGAEAPVIRTYTLSVAPSDPTY
RISVKRDGAVSQHLHDRLRVGDIVEARAPDGGFTLDAGAARPAVLLAGGVGITPLLAMLRHVVYEGLRTRRIRPTTLVYA
ARTRAERAFEGELSELAAAAQGAVRIVRVLSDVSDARPGTDYEAAGRIDMALLTRFLAFADYDFYLCGPPAFTQALYDGL
RGYNVADDRIHAEAFGPSGLVRNAGTAVAARPRPPAATETVPVAFLDTGKEARWTPNSGSLLDLAEARGLAPDFSCREGS
CGTCRTKLLAGAVTYLREPSAPVAEDEVLICSAVPAEGTGRVQLAL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG3576

COG function: function code R; Predicted flavin-nucleotide-binding protein structurally related to pyridoxine 5'-phosphate oxidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=248, Percent_Identity=31.8548387096774, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787658, Length=355, Percent_Identity=26.4788732394366, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787098, Length=347, Percent_Identity=26.2247838616715, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788104, Length=352, Percent_Identity=24.4318181818182, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321673, Length=265, Percent_Identity=30.188679245283, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 73641; Mature: 73510

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGQTFATLPTWHAGETSIQESVGAADRMAVIGQRVVRDFMPDQHRAFYAQIPFIVLGS
CCCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEC
VDRQGDAWATLLAGRPGFITSPSATTLDIAARPDPADPASEGTRAGDAIGLLGIELSSRR
CCCCCCCEEEEECCCCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECHHH
RNRVNGLVRSTDGGVLHFDVDQSFGNCPQYIQLRDMTFVGEPGVPVPVSAEESAELDASA
HHHHCCEEEECCCCEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCH
RALIAAADTFFVASYADREGRRQVDVSHRGGKAGFVRVAADGTLTIPDFAGNLFFATLGN
HEEEEEHHHEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHH
ILLNGRAGLVFTDFETGDLLQLTGDAAVILDSPEIAAFQGAERLWTFRPRRVIRRRGALP
HEECCCCCEEEEECCCCCEEEECCCEEEEECCCCHHHHCCCHHHHCCCHHHHHHHCCCCC
LRWTFRADGWSPNALMTGDWAEAADRVRATGRATRWRPFRVAKIVDESRTIRSFHLAPDD
EEEEEEECCCCCCCEECCCHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCCEEEEECCCCC
GAGQLPHRAGQHLPIRVTLPGAEAPVIRTYTLSVAPSDPTYRISVKRDGAVSQHLHDRLR
CCCCCCHHCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHH
VGDIVEARAPDGGFTLDAGAARPAVLLAGGVGITPLLAMLRHVVYEGLRTRRIRPTTLVY
HCCEEECCCCCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
AARTRAERAFEGELSELAAAAQGAVRIVRVLSDVSDARPGTDYEAAGRIDMALLTRFLAF
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
ADYDFYLCGPPAFTQALYDGLRGYNVADDRIHAEAFGPSGLVRNAGTAVAARPRPPAATE
HCCCEEEECCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHCCCCCEEEECCCCCCCCC
TVPVAFLDTGKEARWTPNSGSLLDLAEARGLAPDFSCREGSCGTCRTKLLAGAVTYLREP
CEEEEEEECCCCCEECCCCCCEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
SAPVAEDEVLICSAVPAEGTGRVQLAL
CCCCCCCCEEEEEECCCCCCCCEEEEC
>Mature Secondary Structure 
ADGQTFATLPTWHAGETSIQESVGAADRMAVIGQRVVRDFMPDQHRAFYAQIPFIVLGS
CCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEC
VDRQGDAWATLLAGRPGFITSPSATTLDIAARPDPADPASEGTRAGDAIGLLGIELSSRR
CCCCCCCEEEEECCCCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECHHH
RNRVNGLVRSTDGGVLHFDVDQSFGNCPQYIQLRDMTFVGEPGVPVPVSAEESAELDASA
HHHHCCEEEECCCCEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCH
RALIAAADTFFVASYADREGRRQVDVSHRGGKAGFVRVAADGTLTIPDFAGNLFFATLGN
HEEEEEHHHEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHH
ILLNGRAGLVFTDFETGDLLQLTGDAAVILDSPEIAAFQGAERLWTFRPRRVIRRRGALP
HEECCCCCEEEEECCCCCEEEECCCEEEEECCCCHHHHCCCHHHHCCCHHHHHHHCCCCC
LRWTFRADGWSPNALMTGDWAEAADRVRATGRATRWRPFRVAKIVDESRTIRSFHLAPDD
EEEEEEECCCCCCCEECCCHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCCEEEEECCCCC
GAGQLPHRAGQHLPIRVTLPGAEAPVIRTYTLSVAPSDPTYRISVKRDGAVSQHLHDRLR
CCCCCCHHCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHH
VGDIVEARAPDGGFTLDAGAARPAVLLAGGVGITPLLAMLRHVVYEGLRTRRIRPTTLVY
HCCEEECCCCCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
AARTRAERAFEGELSELAAAAQGAVRIVRVLSDVSDARPGTDYEAAGRIDMALLTRFLAF
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
ADYDFYLCGPPAFTQALYDGLRGYNVADDRIHAEAFGPSGLVRNAGTAVAARPRPPAATE
HCCCEEEECCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHCCCCCEEEECCCCCCCCC
TVPVAFLDTGKEARWTPNSGSLLDLAEARGLAPDFSCREGSCGTCRTKLLAGAVTYLREP
CEEEEEEECCCCCEECCCCCCEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
SAPVAEDEVLICSAVPAEGTGRVQLAL
CCCCCCCCEEEEEECCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8682784; 9384377 [H]