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The map label for this gene is yoaE [H]

Identifier: 170749641

GI number: 170749641

Start: 3429786

End: 3431825

Strand: Reverse

Name: yoaE [H]

Synonym: Mrad2831_3238

Alternate gene names: 170749641

Gene position: 3431825-3429786 (Counterclockwise)

Preceding gene: 170749642

Following gene: 170749637

Centisome position: 56.46

GC content: 75.0

Gene sequence:

>2040_bases
TTGGTCCGGACCGTCTGCCCGCACGATTGTCCCTCTGCCTGCAGCCTCGACGTGCAGGTCGCGGACGGGCGCGTCGTGTC
GGTCCGGGGCGGCGACAATCCCTACACGGCCGGGGTGATCTGCGCGAAGGTCAGCCGCTACGGCGAGCGCGTCCACCACC
CGGACCGGATCCTCCACCCGCTGGAGCGCGTCGGGCCGAAGGGCGGCGCGGCGTGGCGCCGGATCTCCTGGGACGCGGCG
CTCGACCGGCTGGCGGCGGCCTTCTCGGAGACGGCGGACCGTTGGGGGGCGGAGGCGGTCTGGCCGTACAATTCGGGCGG
GACCATGGGCCTCGTCCAGCGCGACGGTATCCACCGGCTCACCCACGTGATGGGCTACTCGCGGCGCAAGCGCACCATCT
GCACGGCGGTCGCGATCGCGGGCTGGAGCGCCGGGGTCGGCCGGATCGCCGGCCCGGATCCCCGCGAGATGGCGCTCTCC
GACCTGATCGTTGTCTGGGGCGGCAACCCGGTGAGCACCCAGGTCAACGCGATGACCCACATCAGCCGCGCCCGCAAGAC
ACGGGGCGCCAAGCTGGTGGTGATCGATCCGTACCGCACCGGGACCGCGGCGGCGGCCGACCTCCACCTCGCGCTCCGGC
CCGGCACCGACGGGGCGCTCGCCTGCGCGGTGCTCCACGTCCTGTTCCGCGACGGCCACGCGGACCGGGCCTACCTCGCC
GCCCAGGCCGAGGACACGGCGGCGCTGGAGGCGCATCTGGCGTCGCGCACCCCCGAATGGGCGGCGGCGATCACCGGCCT
GGAGCCCGCCGCGATCGAGGCCTTCGCGGCCCTCTACGGGCGCACGCCGCGCAGCTACATCCGCTGCGGCTTCGGCTTCA
CCCGCAGCCGCAACGGCGCGGTGAACCTGCACGCGGTGACCTGCCTGCCGACCGTCACCGGCGCCTGGCAGCACGAGGGC
GGCGGCGCCCTCTGGCAGCACGCCGCGATCTTCAACTGGGACAAGACCCTGACGGAGGGGCTCGACGCCAGGGCGCCCGG
CGTGCGCGAGCTCGACATGAGCCGGATCGGCGCCGTGCTCACCGACGATCCCGACGCCCTGCTGGGCGGCCCGCCGGTCC
GCGCCATGCTGATCCAGTCGGCCAATCCCGCGGTCTCCGCGCCCGACAGCGCCCGGGTGCGCGCGGGGCTGACGCGGCCG
GACGTGTTCGTGGCCGTGCACGAGCAGTTCATGACCGAGACGGCCGCGCTCGCCGACCTCGTGCTGCCGGCCACCACCTT
CCTGGAACACGACGACATCTACGGCGCCGGCGGCCACAGCCACATCCAGGCCGGGCCGGCGATCCTGGAGCCGCCGGGCG
AGTGCCGCTCGAACCACGCGCTCCTGTCGGGGCTCGCCGCCCGGCTCGGCGCGGACCACGCGGGCTTCCGCCTGACCGCC
CGGGACCTCGCCGACGCCACCCTCGCGCGCTCCGGCTGGGGCGGTCTCGACAGGCTGGAGGCGGAGGGCTGGATCGACGC
GCAGCCGGATTTCGCCGAGAGCCACTTCCTGACCGGGTTCGGCCATCCCGACGGGCGCTTCCGCTTCGCCCCCGACTGGG
CGGCGCTGGGTCCGCGCGCGGCCGGCATGCCGGCCCTCCCGGATCACTGGCCGGAGGCCGAGCCCGCCGACGCGGAGCAC
CCGTTCCGGATGATCGCCCCGCCGGCCCGTCAGTTCCTCAACGCGACCTTCACCAACGTGCCCGAGTCGCTCCGCCGCGA
GGGGCGGCCGACCTGCCTGATCCACCCGGCGGACGGCGTGCGGCTCGGGATCGGTACGGGCGACGCCGTCGAGGTCGGCA
ATGCCCGCGGTCGGGTCCGGTTGCACGCGCGGCTCGCCGAGGGGCAGCAGCCGGGCGTCGTGGTGGTCGAGAGCCTGTGG
CCGAGCGCGGCCTTCGCGGGCGGGGACGGGGGCATCAACACGCTGATCGGGTCGGCGCCGGCCGCGCCCAACGACGGTGC
CGCCTTCCACGACACTGCGGTCTGGGTTCGAGCGGCCTGA

Upstream 100 bases:

>100_bases
CCCGAGAGGCGGCGGCATGCGCGCCTTGCGGTCTTCCGGCCGAAGGCCCAGAACCGCACGGTCCTTGCTTCCCCATGCTG
GCGCCACGGGAGTTCGTCGC

Downstream 100 bases:

>100_bases
GCCGGGCGCTCGGGCCGTCGAAACTCACGCTCCTTTTCCGGTGCCCCCCTCGCAGAAGCCGGGGACGGACAATCGACAGC
AATCCGTTGCCCGGAATCCC

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MVRTVCPHDCPSACSLDVQVADGRVVSVRGGDNPYTAGVICAKVSRYGERVHHPDRILHPLERVGPKGGAAWRRISWDAA
LDRLAAAFSETADRWGAEAVWPYNSGGTMGLVQRDGIHRLTHVMGYSRRKRTICTAVAIAGWSAGVGRIAGPDPREMALS
DLIVVWGGNPVSTQVNAMTHISRARKTRGAKLVVIDPYRTGTAAAADLHLALRPGTDGALACAVLHVLFRDGHADRAYLA
AQAEDTAALEAHLASRTPEWAAAITGLEPAAIEAFAALYGRTPRSYIRCGFGFTRSRNGAVNLHAVTCLPTVTGAWQHEG
GGALWQHAAIFNWDKTLTEGLDARAPGVRELDMSRIGAVLTDDPDALLGGPPVRAMLIQSANPAVSAPDSARVRAGLTRP
DVFVAVHEQFMTETAALADLVLPATTFLEHDDIYGAGGHSHIQAGPAILEPPGECRSNHALLSGLAARLGADHAGFRLTA
RDLADATLARSGWGGLDRLEAEGWIDAQPDFAESHFLTGFGHPDGRFRFAPDWAALGPRAAGMPALPDHWPEAEPADAEH
PFRMIAPPARQFLNATFTNVPESLRREGRPTCLIHPADGVRLGIGTGDAVEVGNARGRVRLHARLAEGQQPGVVVVESLW
PSAAFAGGDGGINTLIGSAPAAPNDGAAFHDTAVWVRAA

Sequences:

>Translated_679_residues
MVRTVCPHDCPSACSLDVQVADGRVVSVRGGDNPYTAGVICAKVSRYGERVHHPDRILHPLERVGPKGGAAWRRISWDAA
LDRLAAAFSETADRWGAEAVWPYNSGGTMGLVQRDGIHRLTHVMGYSRRKRTICTAVAIAGWSAGVGRIAGPDPREMALS
DLIVVWGGNPVSTQVNAMTHISRARKTRGAKLVVIDPYRTGTAAAADLHLALRPGTDGALACAVLHVLFRDGHADRAYLA
AQAEDTAALEAHLASRTPEWAAAITGLEPAAIEAFAALYGRTPRSYIRCGFGFTRSRNGAVNLHAVTCLPTVTGAWQHEG
GGALWQHAAIFNWDKTLTEGLDARAPGVRELDMSRIGAVLTDDPDALLGGPPVRAMLIQSANPAVSAPDSARVRAGLTRP
DVFVAVHEQFMTETAALADLVLPATTFLEHDDIYGAGGHSHIQAGPAILEPPGECRSNHALLSGLAARLGADHAGFRLTA
RDLADATLARSGWGGLDRLEAEGWIDAQPDFAESHFLTGFGHPDGRFRFAPDWAALGPRAAGMPALPDHWPEAEPADAEH
PFRMIAPPARQFLNATFTNVPESLRREGRPTCLIHPADGVRLGIGTGDAVEVGNARGRVRLHARLAEGQQPGVVVVESLW
PSAAFAGGDGGINTLIGSAPAAPNDGAAFHDTAVWVRAA
>Mature_679_residues
MVRTVCPHDCPSACSLDVQVADGRVVSVRGGDNPYTAGVICAKVSRYGERVHHPDRILHPLERVGPKGGAAWRRISWDAA
LDRLAAAFSETADRWGAEAVWPYNSGGTMGLVQRDGIHRLTHVMGYSRRKRTICTAVAIAGWSAGVGRIAGPDPREMALS
DLIVVWGGNPVSTQVNAMTHISRARKTRGAKLVVIDPYRTGTAAAADLHLALRPGTDGALACAVLHVLFRDGHADRAYLA
AQAEDTAALEAHLASRTPEWAAAITGLEPAAIEAFAALYGRTPRSYIRCGFGFTRSRNGAVNLHAVTCLPTVTGAWQHEG
GGALWQHAAIFNWDKTLTEGLDARAPGVRELDMSRIGAVLTDDPDALLGGPPVRAMLIQSANPAVSAPDSARVRAGLTRP
DVFVAVHEQFMTETAALADLVLPATTFLEHDDIYGAGGHSHIQAGPAILEPPGECRSNHALLSGLAARLGADHAGFRLTA
RDLADATLARSGWGGLDRLEAEGWIDAQPDFAESHFLTGFGHPDGRFRFAPDWAALGPRAAGMPALPDHWPEAEPADAEH
PFRMIAPPARQFLNATFTNVPESLRREGRPTCLIHPADGVRLGIGTGDAVEVGNARGRVRLHARLAEGQQPGVVVVESLW
PSAAFAGGDGGINTLIGSAPAAPNDGAAFHDTAVWVRAA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds (By Similarity). [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=766, Percent_Identity=25.4569190600522, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI87081797, Length=766, Percent_Identity=24.4125326370757, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI171474008, Length=770, Percent_Identity=24.6753246753247, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI3868721, Length=687, Percent_Identity=24.8908296943231, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI145693196, Length=710, Percent_Identity=25.4929577464789, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI87081994, Length=745, Percent_Identity=23.2214765100671, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1787231, Length=731, Percent_Identity=22.2982216142271, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 71898; Mature: 71898

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRTVCPHDCPSACSLDVQVADGRVVSVRGGDNPYTAGVICAKVSRYGERVHHPDRILHP
CCCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
LERVGPKGGAAWRRISWDAALDRLAAAFSETADRWGAEAVWPYNSGGTMGLVQRDGIHRL
HHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCHHHH
THVMGYSRRKRTICTAVAIAGWSAGVGRIAGPDPREMALSDLIVVWGGNPVSTQVNAMTH
HHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCCHHHHHHHH
ISRARKTRGAKLVVIDPYRTGTAAAADLHLALRPGTDGALACAVLHVLFRDGHADRAYLA
HHHHHHHCCCEEEEECCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEE
AQAEDTAALEAHLASRTPEWAAAITGLEPAAIEAFAALYGRTPRSYIRCGFGFTRSRNGA
ECCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCE
VNLHAVTCLPTVTGAWQHEGGGALWQHAAIFNWDKTLTEGLDARAPGVRELDMSRIGAVL
EEEEEEEEECCCCCCCEECCCCCHHHCCEEEECCHHHHCCCCCCCCCCCHHCHHHCCEEE
TDDPDALLGGPPVRAMLIQSANPAVSAPDSARVRAGLTRPDVFVAVHEQFMTETAALADL
ECCCCHHCCCCCCEEEEEECCCCCCCCCCCCHHEECCCCCCEEEEHHHHHHHHHHHHHHH
VLPATTFLEHDDIYGAGGHSHIQAGPAILEPPGECRSNHALLSGLAARLGADHAGFRLTA
HHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHCCCHHHHHHHHHHHCCCCCCCEEEH
RDLADATLARSGWGGLDRLEAEGWIDAQPDFAESHFLTGFGHPDGRFRFAPDWAALGPRA
HHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCHHHCCCCC
AGMPALPDHWPEAEPADAEHPFRMIAPPARQFLNATFTNVPESLRREGRPTCLIHPADGV
CCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCC
RLGIGTGDAVEVGNARGRVRLHARLAEGQQPGVVVVESLWPSAAFAGGDGGINTLIGSAP
EEECCCCCCEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCEECCCCCCHHHHHCCCC
AAPNDGAAFHDTAVWVRAA
CCCCCCCEEECEEEEEEEC
>Mature Secondary Structure
MVRTVCPHDCPSACSLDVQVADGRVVSVRGGDNPYTAGVICAKVSRYGERVHHPDRILHP
CCCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
LERVGPKGGAAWRRISWDAALDRLAAAFSETADRWGAEAVWPYNSGGTMGLVQRDGIHRL
HHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCHHHH
THVMGYSRRKRTICTAVAIAGWSAGVGRIAGPDPREMALSDLIVVWGGNPVSTQVNAMTH
HHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCCHHHHHHHH
ISRARKTRGAKLVVIDPYRTGTAAAADLHLALRPGTDGALACAVLHVLFRDGHADRAYLA
HHHHHHHCCCEEEEECCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEE
AQAEDTAALEAHLASRTPEWAAAITGLEPAAIEAFAALYGRTPRSYIRCGFGFTRSRNGA
ECCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCE
VNLHAVTCLPTVTGAWQHEGGGALWQHAAIFNWDKTLTEGLDARAPGVRELDMSRIGAVL
EEEEEEEEECCCCCCCEECCCCCHHHCCEEEECCHHHHCCCCCCCCCCCHHCHHHCCEEE
TDDPDALLGGPPVRAMLIQSANPAVSAPDSARVRAGLTRPDVFVAVHEQFMTETAALADL
ECCCCHHCCCCCCEEEEEECCCCCCCCCCCCHHEECCCCCCEEEEHHHHHHHHHHHHHHH
VLPATTFLEHDDIYGAGGHSHIQAGPAILEPPGECRSNHALLSGLAARLGADHAGFRLTA
HHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHCCCHHHHHHHHHHHCCCCCCCEEEH
RDLADATLARSGWGGLDRLEAEGWIDAQPDFAESHFLTGFGHPDGRFRFAPDWAALGPRA
HHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCHHHCCCCC
AGMPALPDHWPEAEPADAEHPFRMIAPPARQFLNATFTNVPESLRREGRPTCLIHPADGV
CCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCC
RLGIGTGDAVEVGNARGRVRLHARLAEGQQPGVVVVESLWPSAAFAGGDGGINTLIGSAP
EEECCCCCCEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCEECCCCCCHHHHHCCCC
AAPNDGAAFHDTAVWVRAA
CCCCCCCEEECEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]