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The map label for this gene is potD-A [H]

Identifier: 170749635

GI number: 170749635

Start: 3419276

End: 3420307

Strand: Reverse

Name: potD-A [H]

Synonym: Mrad2831_3232

Alternate gene names: 170749635

Gene position: 3420307-3419276 (Counterclockwise)

Preceding gene: 170749636

Following gene: 170749634

Centisome position: 56.28

GC content: 65.02

Gene sequence:

>1032_bases
ATGCGTCTTCTGACCCCGCTCCTCGCGGCCTCCGCCCTGGTCGCATCCATCATCCCGGTGCAGGCGGCCGAGAAGCTCGT
CGTCAGCACCTGGGGCGGCAGCTTCCGCGATCTGATCGACGAGGCCATCGCCACGAAGTTCAAGGCGGAGACCGGCGCCG
AGGTCGAGTACATCACCGGCGGTACCATCGACCGGCTCAACAAGGCCAAGCTCGCCAAGGACGCGCCCGAGAGCGATGTG
ACCTTCACGACCGCCCATGTCGGCTGGCTCTACGTCAATGACGGCCTGTTCGAGACGCTCGACATGGCCAAGATCCCGAA
CGCGGCGAACCTCGTCGAGCAGGCCCGGGTCAGCCCGGCGCATCTCGGCACTTGGGGCTACGTCTACACGATCGGCTACC
GCGCGGACCTGACGCCCAAGGGCATGACCTTCGGCTCCTGGGCCGATCTCTGGGACCCGAAGCTCAAGAACAGCCTGGCC
GCGCCCGATTTCGACCCGAGCCACATCATCGCCGTCTCGGCGCTGCTCTCGGGCGGCGACCCGGCGACCTGGGAGAAGGG
GCAGGCCAAGCTGGAGGCGCTGAAGCCGAACTTCCGGGCCTTCTACACCAACGATGCCAACAGCCAGCAGCTGATCGCCA
CCGGCGAGACGCCCGTTCAGGTCGTGCTGACCATGAACGCCTACTACATGAAGGGCCAGGACGTGCCGGTCACCGTGGTG
ATCCCGAAGGAGGGTGGGGTTCTCGGCATCGACACGGTCGCGATCAACAAGGGTTCCAAGAAGGCTGAGCTCGCCTACAA
GTTCATCAACATCGCTCTCGATCCCGAAGTACAGGCCAAGATCGCGCAGCTGAAGAAGGGCAGCCCGACCGTCACCAACG
CCAAGGTCGATCCCGAGATCGCCAAGCTGCCGGGTGTGTTCACCACCGCCGAGCAGTGGAAGACCCAGGCGATCAACATC
GACCCGAAGCTCCGGGCCGAGAAGACCGCGCTCTGGCGCAAGTGGTTCGCCGAGAACATCATGGCGCGCTAG

Upstream 100 bases:

>100_bases
ATCTCGATCGAAACCGGAACAGTTTCTCCAGGATAAAATACATCTCTGGCATTCGATTTGCTCGGCGCGCGGTACTGCAG
AGAAAGGAACTGTCGACGTG

Downstream 100 bases:

>100_bases
GGCCCGCGCCATGCGCCTCGCTCGACGGGAAGCCTCATGACACCCGCGATCCGCCGCCGGCCCTTCCTGCCGCTGTTCGT
GACGCCGGCGGTGCTGGTCG

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRLLTPLLAASALVASIIPVQAAEKLVVSTWGGSFRDLIDEAIATKFKAETGAEVEYITGGTIDRLNKAKLAKDAPESDV
TFTTAHVGWLYVNDGLFETLDMAKIPNAANLVEQARVSPAHLGTWGYVYTIGYRADLTPKGMTFGSWADLWDPKLKNSLA
APDFDPSHIIAVSALLSGGDPATWEKGQAKLEALKPNFRAFYTNDANSQQLIATGETPVQVVLTMNAYYMKGQDVPVTVV
IPKEGGVLGIDTVAINKGSKKAELAYKFINIALDPEVQAKIAQLKKGSPTVTNAKVDPEIAKLPGVFTTAEQWKTQAINI
DPKLRAEKTALWRKWFAENIMAR

Sequences:

>Translated_343_residues
MRLLTPLLAASALVASIIPVQAAEKLVVSTWGGSFRDLIDEAIATKFKAETGAEVEYITGGTIDRLNKAKLAKDAPESDV
TFTTAHVGWLYVNDGLFETLDMAKIPNAANLVEQARVSPAHLGTWGYVYTIGYRADLTPKGMTFGSWADLWDPKLKNSLA
APDFDPSHIIAVSALLSGGDPATWEKGQAKLEALKPNFRAFYTNDANSQQLIATGETPVQVVLTMNAYYMKGQDVPVTVV
IPKEGGVLGIDTVAINKGSKKAELAYKFINIALDPEVQAKIAQLKKGSPTVTNAKVDPEIAKLPGVFTTAEQWKTQAINI
DPKLRAEKTALWRKWFAENIMAR
>Mature_343_residues
MRLLTPLLAASALVASIIPVQAAEKLVVSTWGGSFRDLIDEAIATKFKAETGAEVEYITGGTIDRLNKAKLAKDAPESDV
TFTTAHVGWLYVNDGLFETLDMAKIPNAANLVEQARVSPAHLGTWGYVYTIGYRADLTPKGMTFGSWADLWDPKLKNSLA
APDFDPSHIIAVSALLSGGDPATWEKGQAKLEALKPNFRAFYTNDANSQQLIATGETPVQVVLTMNAYYMKGQDVPVTVV
IPKEGGVLGIDTVAINKGSKKAELAYKFINIALDPEVQAKIAQLKKGSPTVTNAKVDPEIAKLPGVFTTAEQWKTQAINI
DPKLRAEKTALWRKWFAENIMAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=295, Percent_Identity=29.8305084745763, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787078, Length=358, Percent_Identity=26.536312849162, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37231; Mature: 37231

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLTPLLAASALVASIIPVQAAEKLVVSTWGGSFRDLIDEAIATKFKAETGAEVEYITG
CCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEC
GTIDRLNKAKLAKDAPESDVTFTTAHVGWLYVNDGLFETLDMAKIPNAANLVEQARVSPA
CCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCC
HLGTWGYVYTIGYRADLTPKGMTFGSWADLWDPKLKNSLAAPDFDPSHIIAVSALLSGGD
CCCCCEEEEEEEEEECCCCCCCCCCCCHHHCCHHHHCCCCCCCCCCHHHHEEEHHHCCCC
PATWEKGQAKLEALKPNFRAFYTNDANSQQLIATGETPVQVVLTMNAYYMKGQDVPVTVV
CCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEECCCCEEEEEEEEEEEECCCCCEEEEE
IPKEGGVLGIDTVAINKGSKKAELAYKFINIALDPEVQAKIAQLKKGSPTVTNAKVDPEI
EECCCCEEEEEEEEECCCCCHHHEEEEEEEEEECCHHHHHHHHHCCCCCCEECCCCCCHH
AKLPGVFTTAEQWKTQAINIDPKLRAEKTALWRKWFAENIMAR
HHCCCCEECHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLLTPLLAASALVASIIPVQAAEKLVVSTWGGSFRDLIDEAIATKFKAETGAEVEYITG
CCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEC
GTIDRLNKAKLAKDAPESDVTFTTAHVGWLYVNDGLFETLDMAKIPNAANLVEQARVSPA
CCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCC
HLGTWGYVYTIGYRADLTPKGMTFGSWADLWDPKLKNSLAAPDFDPSHIIAVSALLSGGD
CCCCCEEEEEEEEEECCCCCCCCCCCCHHHCCHHHHCCCCCCCCCCHHHHEEEHHHCCCC
PATWEKGQAKLEALKPNFRAFYTNDANSQQLIATGETPVQVVLTMNAYYMKGQDVPVTVV
CCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEEECCCCEEEEEEEEEEEECCCCCEEEEE
IPKEGGVLGIDTVAINKGSKKAELAYKFINIALDPEVQAKIAQLKKGSPTVTNAKVDPEI
EECCCCEEEEEEEEECCCCCHHHEEEEEEEEEECCHHHHHHHHHCCCCCCEECCCCCCHH
AKLPGVFTTAEQWKTQAINIDPKLRAEKTALWRKWFAENIMAR
HHCCCCEECHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]