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The map label for this gene is potH [H]

Identifier: 170749634

GI number: 170749634

Start: 3418376

End: 3419239

Strand: Reverse

Name: potH [H]

Synonym: Mrad2831_3231

Alternate gene names: 170749634

Gene position: 3419239-3418376 (Counterclockwise)

Preceding gene: 170749635

Following gene: 170749633

Centisome position: 56.26

GC content: 70.25

Gene sequence:

>864_bases
ATGACACCCGCGATCCGCCGCCGGCCCTTCCTGCCGCTGTTCGTGACGCCGGCGGTGCTGGTCGCCGCCGCCATCGTGGC
CGCCATGGCGGCGATCCTGCGCTACAGCCTGCACGCCTACGTGCCGGGATCCCTCGACGTCGGCGGTCTCACGCTGGAGA
ACTTCTCCGATCTCGGCCGCCCGATCGTGCTCCGGGCGCTGCGCGACACGGTGCTGATCTGCTTCGAGACGGCGCTGATC
ACCCTGGTCGTCGGCTACCCCGTCGCCTACGCCCTGGTGCGCACCCGCTCGCTGCTGCTGAAGTCCGTCATCCTGATCGT
CGCGGTGACCCCGCTCTTCCTCGGCGAGGTGGTGCGCACCTATGCCTGGACGGTGGTGCTCGGCAATTCCGGCTTCGTGA
ACGCCACCTTGCGCCAGCTCGGCCTGATCGAGCGGCCGATCCAGCTCATGTTCACCGAGCTCGGGGTGATCGTCGCCCTC
GTGCACGTCACGCTGCCGGTGATGGTCATCATGCTGGCCGCGGCCCTCGCGCATATCGACCGCGACTACGAGCGCGCGGC
CCAGAGCCTCGGCGCCGGGCCGGTGCGCACGTTCCTCACCGTGACGCTGCCGCTCTCGATGCCCGGCGTGGTGGCGGGCT
TCACCACCGCCTTCGCCTGGACGTTCAGCGCCTTCGCGACGCCCCAGGTGATCGGCGGCGGAAAGGTCGGGGTCGTCTCG
ACCCTGGTCTACCAGCTCGGCCTGTCCTCCTTCAATTTCCCGGTCGCGGCGTCCCTGAGCCTCGCCGGCCTCGTCCTGGC
GCTCGGAACGATGTGGCTCGTGCGCCGCTGCGCCCGCCCGCTCGAAGCGCTCGGAGGCCACTGA

Upstream 100 bases:

>100_bases
GCTCCGGGCCGAGAAGACCGCGCTCTGGCGCAAGTGGTTCGCCGAGAACATCATGGCGCGCTAGGGCCCGCGCCATGCGC
CTCGCTCGACGGGAAGCCTC

Downstream 100 bases:

>100_bases
TGCGCCGCGGCCTCCGCACCCGGCTTCTCGGGATCTCCGGCAGGGTCCTCGTCGGGCTGATCCTCGCCTTCATCGTGCTG
CCGGCCTTCGTGGTGACGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTPAIRRRPFLPLFVTPAVLVAAAIVAAMAAILRYSLHAYVPGSLDVGGLTLENFSDLGRPIVLRALRDTVLICFETALI
TLVVGYPVAYALVRTRSLLLKSVILIVAVTPLFLGEVVRTYAWTVVLGNSGFVNATLRQLGLIERPIQLMFTELGVIVAL
VHVTLPVMVIMLAAALAHIDRDYERAAQSLGAGPVRTFLTVTLPLSMPGVVAGFTTAFAWTFSAFATPQVIGGGKVGVVS
TLVYQLGLSSFNFPVAASLSLAGLVLALGTMWLVRRCARPLEALGGH

Sequences:

>Translated_287_residues
MTPAIRRRPFLPLFVTPAVLVAAAIVAAMAAILRYSLHAYVPGSLDVGGLTLENFSDLGRPIVLRALRDTVLICFETALI
TLVVGYPVAYALVRTRSLLLKSVILIVAVTPLFLGEVVRTYAWTVVLGNSGFVNATLRQLGLIERPIQLMFTELGVIVAL
VHVTLPVMVIMLAAALAHIDRDYERAAQSLGAGPVRTFLTVTLPLSMPGVVAGFTTAFAWTFSAFATPQVIGGGKVGVVS
TLVYQLGLSSFNFPVAASLSLAGLVLALGTMWLVRRCARPLEALGGH
>Mature_286_residues
TPAIRRRPFLPLFVTPAVLVAAAIVAAMAAILRYSLHAYVPGSLDVGGLTLENFSDLGRPIVLRALRDTVLICFETALIT
LVVGYPVAYALVRTRSLLLKSVILIVAVTPLFLGEVVRTYAWTVVLGNSGFVNATLRQLGLIERPIQLMFTELGVIVALV
HVTLPVMVIMLAAALAHIDRDYERAAQSLGAGPVRTFLTVTLPLSMPGVVAGFTTAFAWTFSAFATPQVIGGGKVGVVST
LVYQLGLSSFNFPVAASLSLAGLVLALGTMWLVRRCARPLEALGGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=254, Percent_Identity=28.3464566929134, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=29.9145299145299, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787713, Length=244, Percent_Identity=29.0983606557377, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1786980, Length=102, Percent_Identity=35.2941176470588, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30522; Mature: 30391

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAIRRRPFLPLFVTPAVLVAAAIVAAMAAILRYSLHAYVPGSLDVGGLTLENFSDLGR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHCCH
PIVLRALRDTVLICFETALITLVVGYPVAYALVRTRSLLLKSVILIVAVTPLFLGEVVRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAWTVVLGNSGFVNATLRQLGLIERPIQLMFTELGVIVALVHVTLPVMVIMLAAALAHID
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDYERAAQSLGAGPVRTFLTVTLPLSMPGVVAGFTTAFAWTFSAFATPQVIGGGKVGVVS
HHHHHHHHHCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
TLVYQLGLSSFNFPVAASLSLAGLVLALGTMWLVRRCARPLEALGGH
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPAIRRRPFLPLFVTPAVLVAAAIVAAMAAILRYSLHAYVPGSLDVGGLTLENFSDLGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHCCH
PIVLRALRDTVLICFETALITLVVGYPVAYALVRTRSLLLKSVILIVAVTPLFLGEVVRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAWTVVLGNSGFVNATLRQLGLIERPIQLMFTELGVIVALVHVTLPVMVIMLAAALAHID
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDYERAAQSLGAGPVRTFLTVTLPLSMPGVVAGFTTAFAWTFSAFATPQVIGGGKVGVVS
HHHHHHHHHCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
TLVYQLGLSSFNFPVAASLSLAGLVLALGTMWLVRRCARPLEALGGH
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]