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The map label for this gene is ghrA [H]

Identifier: 170749615

GI number: 170749615

Start: 3397297

End: 3398229

Strand: Reverse

Name: ghrA [H]

Synonym: Mrad2831_3212

Alternate gene names: 170749615

Gene position: 3398229-3397297 (Counterclockwise)

Preceding gene: 170749616

Following gene: 170749612

Centisome position: 55.91

GC content: 71.06

Gene sequence:

>933_bases
ATGGTGATCCCCGTCGTTCTCAAGACCGTTCCGGAGCACGCCCGCGTCTGGCACCGCGTGTTCGCCCAGGAGGCGCCGGA
GCTGAGCCTGGTCGACTGGGCGCACGGCACGCGGTTTCCCGGCGTGAGCGCCTTCGCCGGTTGGCAGCCGCCCGCGGACT
TGGCCGAGCAGATGCCCGACCTGCGCCTCGTGTTCTCCGTCGGCGCCGGGATCGATCACCTGCCGCTCGATGCCCTGCCG
GAGCCCGTGCACATCGTCCGCATGGTCGCCCCCGACGTGACGCGCTCCATGGTCGAGTATGTCACGCTCGGCGTGCTGGC
TCTGCACCGGGACCTCGTTCCTTACGCGAGCGAGACCCGTCGGGGCATCTGGAAGCCGCGGGCGATCCGCACGGCCCGGA
CCACCCAGGTCGGAATCCTCGGCCTGGGCGTGCTCGGGAGCGCGGCGGCCGCGGCCCTGGCCGGGTTCGGCTTCCAAGTT
CGCGGCTGGGCGAGAAGCCCCAAGACGCTGGACGGCGTCACGTGCTTCGCCGGCCGTTCGGCGCTCCCGGATTTCCTGTC
CGGCTGCAACATCCTCGTGTGCCTGCTGCCCCTGACGCCGGAAACGCGCGGACTGCTCGACGGAGACCTGTTCCGGCATC
TGCCGGCCGGGGCCGCGCTCCTGAATGCAGGGCGCGGTGCTCATGTCGTCGAGCGCGACCTGATCGCGGCGTTGGACACG
GGCCAGCTCGGGGCTGCCATTCTCGACGTGCTGTCGGAGGAACCGCCGCCCGCGGATCATCCGCTCCTCGGCCACCCGCG
GATCCTCGTGACTCCTCACAGCGCCAGCGCGAGCCAGCCGGACGATGCGGCGCGTCAGATGATTGCGGCGGTTCGAGCCC
TTCGCGACGGGCAGCCGCTGCTCAACCGCGTTGACCGTCGCCGCGGTTTCTGA

Upstream 100 bases:

>100_bases
GGGGACGCTGCGCCGCCCGGAGGGGCCGAAGGATCGCCGAGGCGGCGCGCCCGGCGCAGCGTCGCGGACCGCCGAACTGC
GCCGGGAGCCGTGTAGGGCG

Downstream 100 bases:

>100_bases
GCGCCGTCGACGATTTCCGGATCAAACCGGTCCGGCCGACGTCCGCTCCGTGCGGCGCGGAGAATCGGGCGGCGGGGTGC
GGCGGCCCGAGTTCCTGTAA

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MVIPVVLKTVPEHARVWHRVFAQEAPELSLVDWAHGTRFPGVSAFAGWQPPADLAEQMPDLRLVFSVGAGIDHLPLDALP
EPVHIVRMVAPDVTRSMVEYVTLGVLALHRDLVPYASETRRGIWKPRAIRTARTTQVGILGLGVLGSAAAAALAGFGFQV
RGWARSPKTLDGVTCFAGRSALPDFLSGCNILVCLLPLTPETRGLLDGDLFRHLPAGAALLNAGRGAHVVERDLIAALDT
GQLGAAILDVLSEEPPPADHPLLGHPRILVTPHSASASQPDDAARQMIAAVRALRDGQPLLNRVDRRRGF

Sequences:

>Translated_310_residues
MVIPVVLKTVPEHARVWHRVFAQEAPELSLVDWAHGTRFPGVSAFAGWQPPADLAEQMPDLRLVFSVGAGIDHLPLDALP
EPVHIVRMVAPDVTRSMVEYVTLGVLALHRDLVPYASETRRGIWKPRAIRTARTTQVGILGLGVLGSAAAAALAGFGFQV
RGWARSPKTLDGVTCFAGRSALPDFLSGCNILVCLLPLTPETRGLLDGDLFRHLPAGAALLNAGRGAHVVERDLIAALDT
GQLGAAILDVLSEEPPPADHPLLGHPRILVTPHSASASQPDDAARQMIAAVRALRDGQPLLNRVDRRRGF
>Mature_310_residues
MVIPVVLKTVPEHARVWHRVFAQEAPELSLVDWAHGTRFPGVSAFAGWQPPADLAEQMPDLRLVFSVGAGIDHLPLDALP
EPVHIVRMVAPDVTRSMVEYVTLGVLALHRDLVPYASETRRGIWKPRAIRTARTTQVGILGLGVLGSAAAAALAGFGFQV
RGWARSPKTLDGVTCFAGRSALPDFLSGCNILVCLLPLTPETRGLLDGDLFRHLPAGAALLNAGRGAHVVERDLIAALDT
GQLGAAILDVLSEEPPPADHPLLGHPRILVTPHSASASQPDDAARQMIAAVRALRDGQPLLNRVDRRRGF

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=251, Percent_Identity=32.6693227091633, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI23308577, Length=233, Percent_Identity=28.755364806867, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI61743967, Length=257, Percent_Identity=28.4046692607004, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4557497, Length=257, Percent_Identity=28.4046692607004, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI145580578, Length=237, Percent_Identity=27.0042194092827, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4557499, Length=237, Percent_Identity=27.0042194092827, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI145580575, Length=237, Percent_Identity=27.0042194092827, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI87081824, Length=300, Percent_Identity=37.6666666666667, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI87082289, Length=241, Percent_Identity=32.3651452282158, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25147481, Length=255, Percent_Identity=28.6274509803922, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=226, Percent_Identity=25.6637168141593, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324964, Length=224, Percent_Identity=25.8928571428571, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320925, Length=199, Percent_Identity=28.643216080402, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322116, Length=199, Percent_Identity=28.643216080402, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585516, Length=289, Percent_Identity=27.3356401384083, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI28574286, Length=174, Percent_Identity=32.183908045977, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571528, Length=227, Percent_Identity=28.6343612334802, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921140, Length=248, Percent_Identity=26.6129032258064, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45552429, Length=175, Percent_Identity=30.8571428571429, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585514, Length=175, Percent_Identity=30.8571428571429, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28574282, Length=175, Percent_Identity=30.8571428571429, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28574284, Length=175, Percent_Identity=30.8571428571429, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551003, Length=175, Percent_Identity=30.8571428571429, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646446, Length=255, Percent_Identity=28.6274509803922, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646448, Length=255, Percent_Identity=28.6274509803922, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646452, Length=255, Percent_Identity=28.6274509803922, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646450, Length=255, Percent_Identity=28.6274509803922, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI62472511, Length=255, Percent_Identity=28.6274509803922, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33212; Mature: 33212

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIPVVLKTVPEHARVWHRVFAQEAPELSLVDWAHGTRFPGVSAFAGWQPPADLAEQMPD
CCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCC
LRLVFSVGAGIDHLPLDALPEPVHIVRMVAPDVTRSMVEYVTLGVLALHRDLVPYASETR
EEEEEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RGIWKPRAIRTARTTQVGILGLGVLGSAAAAALAGFGFQVRGWARSPKTLDGVTCFAGRS
HCCCCCHHHHHHHHCEEHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEECCCCC
ALPDFLSGCNILVCLLPLTPETRGLLDGDLFRHLPAGAALLNAGRGAHVVERDLIAALDT
CCHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCC
GQLGAAILDVLSEEPPPADHPLLGHPRILVTPHSASASQPDDAARQMIAAVRALRDGQPL
CHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHH
LNRVDRRRGF
HHHHHHHCCC
>Mature Secondary Structure
MVIPVVLKTVPEHARVWHRVFAQEAPELSLVDWAHGTRFPGVSAFAGWQPPADLAEQMPD
CCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCC
LRLVFSVGAGIDHLPLDALPEPVHIVRMVAPDVTRSMVEYVTLGVLALHRDLVPYASETR
EEEEEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RGIWKPRAIRTARTTQVGILGLGVLGSAAAAALAGFGFQVRGWARSPKTLDGVTCFAGRS
HCCCCCHHHHHHHHCEEHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEECCCCC
ALPDFLSGCNILVCLLPLTPETRGLLDGDLFRHLPAGAALLNAGRGAHVVERDLIAALDT
CCHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCC
GQLGAAILDVLSEEPPPADHPLLGHPRILVTPHSASASQPDDAARQMIAAVRALRDGQPL
CHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHH
LNRVDRRRGF
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA