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The map label for this gene is hycF [C]

Identifier: 170749599

GI number: 170749599

Start: 3373396

End: 3375423

Strand: Reverse

Name: hycF [C]

Synonym: Mrad2831_3195

Alternate gene names: 170749599

Gene position: 3375423-3373396 (Counterclockwise)

Preceding gene: 170749612

Following gene: 170749595

Centisome position: 55.54

GC content: 72.93

Gene sequence:

>2028_bases
TTGTCCGACCGCCTCGTATTCGCCTGCTCCTGCGAGCGGACGATGTCCCTCGATACCGCCGTCATTGGCCAGGCCTGCGG
CGGCGAGCTTCGCACCGCCGACCAGCTCTGCGGCCGCGAGCTCGATCGCTTCCGGGAGGCCCTGGCGACCGGGCGGCCCG
TCACCGTGTCCTGCACGCTCCAGGCGCCACTGTTCGACGAGGTCGCCGAGGAGGCAGGGGCGGAGGACCGGGTGGTCTAC
GCCAAGATCCGGGAGACCGCCGGCTGGTCGTCCGACGCCGCGGCGGCCGGACCGAAGATGGCCGCCCTGCTCGCCGCGGC
GGCGGAGCCGCTCCCCGATGCCGCCACCGTGGCCCTGGAGAGCCGCGGCGTCGCCTTGATCTACGGGCGCGACGAGGTGG
CGGTCGAGGCCGGCCGGCGGCTCGCCGAGCATCTGGACATCACCGTCCTGCTGACGCGCCCCGGCGAGATTCCGCCGCTG
CACCGCAACGCCTTCCCAGTCTTGCAGGGCACGATCCGGACCGCGAAGGGATATCTCGGTGCGTTCGAGCTCGGCATCGA
CGATTACGCCCTGCCGGCCCCGTCCTCGCGCACGCATCTCGTCTTCGGCGCTCCGCGCAACGGGGCCGTGTCGACCTGCG
ATCTCGTCATCGACCTCTCGGCCGGCGCGCCGCTGTTTCCGGCACACGCGGTGCGGAGCGGTTATCTGCGCGCGGATCCC
GGCGATCCGGCCGCCGTCGAGCGCGTGCTGGCGGAGGCGTCCCATCTGGTCGGCACGTTTGACAAGCCGCGCTTCATCGA
CTTCCACGGTGACTTGTGCGCGCACAGCCGCTCCCGCATCACGGGCTGCACGCGCTGCCTCGATGTCTGCCCGACCGGCG
CGATCGCGCCCGCGGGCGACACCGTGCGGATCGACCCATACGTCTGCGCGGGATGCGGCTCCTGTGCCGCACTCTGCCCG
ACCGGGGCCGCCGCTTACGCGCTGCCGACCTCCGACGCGCTGATGCGCCGGCTGCGCAGCCTGATGCGCGCCTACCGGAC
GGCGGGCGGGCAGAACGCCCTCGTCCTGGTCCACGACGGCGACCACGGCGAGCCGCTGATCGACGCGCTCGGCCGCTACG
GCGACGGCCTCCCCGCCCACGTGCTGCCGGTGCAGGTCAACGAGGTCACGCAGATCGGACCGGAGGTCATCGCGGCCCTG
TTCGCCTACGGCGCGGCCGGAATCCGGGTCCTGGTCCGGGCCCGTGCCAAGCACGACCTCGAGCCCCTGTACCGGACCCT
GACCCTCGGGGAGACGCTGGCGCAGGCGCTGGGCTACGGTGCGGAGGACGGCGGGCGCGTGGTCGAGACCATCGAGACCG
ATGATCCGGATCTCCTGGGCGCCGCCCTCCGGCAGGCCACGCCGGGGCGGCAGGCCGCGGTGCCGGCCGGTCTGCTGTTC
CTGGGGAACAAGCGCGAGGTGCTGCGCCATACCTTCCGCGGCATGCACAGTGCCGCACCGGCACCCGTCGACCGGGTTCC
GCTCGCGGCCGGCGCCCCCTTCGGCGGCCTCGATTTCCGGGTCGCGGACTGCACCCTGTGCCTCGCCTGCGTCAGCGCCT
GCCCGACCCACGCCCTCTCCGACAGTGCCGAGCAGCCGCTTCTCGGCTTCGAGGAGAGCCTCTGCGTGCAGTGCGGCCTC
TGCGCCGCGACCTGCCCGGAGGACGTCATCACCCTGCGCCCGCAGGTGGATTTCGCGGCCTGGGCGGCGCCCCGCCGGGT
CGTCAAGGAGGAAGAGCCCTTCTGCTGCACGCAATGCGCCAAGCCCTTCGGCACGCGCTCGACGATCGAGCGCATCGTCG
AGCGGCTGCGCGAGCGGCACTGGATGTTCAGCGGGGCGGGCGGCGAAGCGCGCCTGCGTGCGCTGACGATGTGCGAGGAT
TGCCGCGTCGAGGTCGCGGTGAACCAGGGCTTCGATCCGCACGCAGCGCCGGAACGCGACAAGCCCCGGACGACCGAGGA
TTACCTGCGCGCGCGCGCGGGCGGCTGA

Upstream 100 bases:

>100_bases
CGCCCGCATGCCGGATCGTTGTTTCGAATGGTTCGAGTATAAGGGACGGGCCCCGCCGCGCGAAGGGGCCTCCCATTCGC
ACTTGTACCCGGGTCCGATC

Downstream 100 bases:

>100_bases
CGGGCGCCTCAGCGAAGCGCGGTGGCGCGTTCGAGGAATTCGATCAGCGCCGCGCGGTCCGCGTCGTTGCCGATCGTCTG
CTCGGGCATCTTCGTCCCGG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: Methyl-Viologen-Reducing Hydrogenase Delta Subunit; 4Fe-4S Ferredoxin; 4fe-4S Ferredoxin; Polyferredoxins; Ferridoxin Protein

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MSDRLVFACSCERTMSLDTAVIGQACGGELRTADQLCGRELDRFREALATGRPVTVSCTLQAPLFDEVAEEAGAEDRVVY
AKIRETAGWSSDAAAAGPKMAALLAAAAEPLPDAATVALESRGVALIYGRDEVAVEAGRRLAEHLDITVLLTRPGEIPPL
HRNAFPVLQGTIRTAKGYLGAFELGIDDYALPAPSSRTHLVFGAPRNGAVSTCDLVIDLSAGAPLFPAHAVRSGYLRADP
GDPAAVERVLAEASHLVGTFDKPRFIDFHGDLCAHSRSRITGCTRCLDVCPTGAIAPAGDTVRIDPYVCAGCGSCAALCP
TGAAAYALPTSDALMRRLRSLMRAYRTAGGQNALVLVHDGDHGEPLIDALGRYGDGLPAHVLPVQVNEVTQIGPEVIAAL
FAYGAAGIRVLVRARAKHDLEPLYRTLTLGETLAQALGYGAEDGGRVVETIETDDPDLLGAALRQATPGRQAAVPAGLLF
LGNKREVLRHTFRGMHSAAPAPVDRVPLAAGAPFGGLDFRVADCTLCLACVSACPTHALSDSAEQPLLGFEESLCVQCGL
CAATCPEDVITLRPQVDFAAWAAPRRVVKEEEPFCCTQCAKPFGTRSTIERIVERLRERHWMFSGAGGEARLRALTMCED
CRVEVAVNQGFDPHAAPERDKPRTTEDYLRARAGG

Sequences:

>Translated_675_residues
MSDRLVFACSCERTMSLDTAVIGQACGGELRTADQLCGRELDRFREALATGRPVTVSCTLQAPLFDEVAEEAGAEDRVVY
AKIRETAGWSSDAAAAGPKMAALLAAAAEPLPDAATVALESRGVALIYGRDEVAVEAGRRLAEHLDITVLLTRPGEIPPL
HRNAFPVLQGTIRTAKGYLGAFELGIDDYALPAPSSRTHLVFGAPRNGAVSTCDLVIDLSAGAPLFPAHAVRSGYLRADP
GDPAAVERVLAEASHLVGTFDKPRFIDFHGDLCAHSRSRITGCTRCLDVCPTGAIAPAGDTVRIDPYVCAGCGSCAALCP
TGAAAYALPTSDALMRRLRSLMRAYRTAGGQNALVLVHDGDHGEPLIDALGRYGDGLPAHVLPVQVNEVTQIGPEVIAAL
FAYGAAGIRVLVRARAKHDLEPLYRTLTLGETLAQALGYGAEDGGRVVETIETDDPDLLGAALRQATPGRQAAVPAGLLF
LGNKREVLRHTFRGMHSAAPAPVDRVPLAAGAPFGGLDFRVADCTLCLACVSACPTHALSDSAEQPLLGFEESLCVQCGL
CAATCPEDVITLRPQVDFAAWAAPRRVVKEEEPFCCTQCAKPFGTRSTIERIVERLRERHWMFSGAGGEARLRALTMCED
CRVEVAVNQGFDPHAAPERDKPRTTEDYLRARAGG
>Mature_674_residues
SDRLVFACSCERTMSLDTAVIGQACGGELRTADQLCGRELDRFREALATGRPVTVSCTLQAPLFDEVAEEAGAEDRVVYA
KIRETAGWSSDAAAAGPKMAALLAAAAEPLPDAATVALESRGVALIYGRDEVAVEAGRRLAEHLDITVLLTRPGEIPPLH
RNAFPVLQGTIRTAKGYLGAFELGIDDYALPAPSSRTHLVFGAPRNGAVSTCDLVIDLSAGAPLFPAHAVRSGYLRADPG
DPAAVERVLAEASHLVGTFDKPRFIDFHGDLCAHSRSRITGCTRCLDVCPTGAIAPAGDTVRIDPYVCAGCGSCAALCPT
GAAAYALPTSDALMRRLRSLMRAYRTAGGQNALVLVHDGDHGEPLIDALGRYGDGLPAHVLPVQVNEVTQIGPEVIAALF
AYGAAGIRVLVRARAKHDLEPLYRTLTLGETLAQALGYGAEDGGRVVETIETDDPDLLGAALRQATPGRQAAVPAGLLFL
GNKREVLRHTFRGMHSAAPAPVDRVPLAAGAPFGGLDFRVADCTLCLACVSACPTHALSDSAEQPLLGFEESLCVQCGLC
AATCPEDVITLRPQVDFAAWAAPRRVVKEEEPFCCTQCAKPFGTRSTIERIVERLRERHWMFSGAGGEARLRALTMCEDC
RVEVAVNQGFDPHAAPERDKPRTTEDYLRARAGG

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 71571; Mature: 71440

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLVFACSCERTMSLDTAVIGQACGGELRTADQLCGRELDRFREALATGRPVTVSCTL
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
QAPLFDEVAEEAGAEDRVVYAKIRETAGWSSDAAAAGPKMAALLAAAAEPLPDAATVALE
CCCHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHEEEEE
SRGVALIYGRDEVAVEAGRRLAEHLDITVLLTRPGEIPPLHRNAFPVLQGTIRTAKGYLG
CCCEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCE
AFELGIDDYALPAPSSRTHLVFGAPRNGAVSTCDLVIDLSAGAPLFPAHAVRSGYLRADP
EEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCEECCC
GDPAAVERVLAEASHLVGTFDKPRFIDFHGDLCAHSRSRITGCTRCLDVCPTGAIAPAGD
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
TVRIDPYVCAGCGSCAALCPTGAAAYALPTSDALMRRLRSLMRAYRTAGGQNALVLVHDG
EEEECCEEECCCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
DHGEPLIDALGRYGDGLPAHVLPVQVNEVTQIGPEVIAALFAYGAAGIRVLVRARAKHDL
CCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
EPLYRTLTLGETLAQALGYGAEDGGRVVETIETDDPDLLGAALRQATPGRQAAVPAGLLF
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCEEE
LGNKREVLRHTFRGMHSAAPAPVDRVPLAAGAPFGGLDFRVADCTLCLACVSACPTHALS
ECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCHHHC
DSAEQPLLGFEESLCVQCGLCAATCPEDVITLRPQVDFAAWAAPRRVVKEEEPFCCTQCA
CCCCCCCCCCCHHHHHHHCCHHHCCCHHHEEECCCCCHHHHHHHHHHHCCCCCCHHHHHC
KPFGTRSTIERIVERLRERHWMFSGAGGEARLRALTMCEDCRVEVAVNQGFDPHAAPERD
CCCCCHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCC
KPRTTEDYLRARAGG
CCCCHHHHHHHCCCC
>Mature Secondary Structure 
SDRLVFACSCERTMSLDTAVIGQACGGELRTADQLCGRELDRFREALATGRPVTVSCTL
CCCEEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
QAPLFDEVAEEAGAEDRVVYAKIRETAGWSSDAAAAGPKMAALLAAAAEPLPDAATVALE
CCCHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHEEEEE
SRGVALIYGRDEVAVEAGRRLAEHLDITVLLTRPGEIPPLHRNAFPVLQGTIRTAKGYLG
CCCEEEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCE
AFELGIDDYALPAPSSRTHLVFGAPRNGAVSTCDLVIDLSAGAPLFPAHAVRSGYLRADP
EEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCEECCC
GDPAAVERVLAEASHLVGTFDKPRFIDFHGDLCAHSRSRITGCTRCLDVCPTGAIAPAGD
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
TVRIDPYVCAGCGSCAALCPTGAAAYALPTSDALMRRLRSLMRAYRTAGGQNALVLVHDG
EEEECCEEECCCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
DHGEPLIDALGRYGDGLPAHVLPVQVNEVTQIGPEVIAALFAYGAAGIRVLVRARAKHDL
CCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
EPLYRTLTLGETLAQALGYGAEDGGRVVETIETDDPDLLGAALRQATPGRQAAVPAGLLF
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCEEE
LGNKREVLRHTFRGMHSAAPAPVDRVPLAAGAPFGGLDFRVADCTLCLACVSACPTHALS
ECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCHHHC
DSAEQPLLGFEESLCVQCGLCAATCPEDVITLRPQVDFAAWAAPRRVVKEEEPFCCTQCA
CCCCCCCCCCCHHHHHHHCCHHHCCCHHHEEECCCCCHHHHHHHHHHHCCCCCCHHHHHC
KPFGTRSTIERIVERLRERHWMFSGAGGEARLRALTMCEDCRVEVAVNQGFDPHAAPERD
CCCCCHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCC
KPRTTEDYLRARAGG
CCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA