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The map label for this gene is pbpG [H]

Identifier: 170749580

GI number: 170749580

Start: 3353875

End: 3356061

Strand: Reverse

Name: pbpG [H]

Synonym: Mrad2831_3175

Alternate gene names: 170749580

Gene position: 3356061-3353875 (Counterclockwise)

Preceding gene: 170749581

Following gene: 170749579

Centisome position: 55.22

GC content: 71.24

Gene sequence:

>2187_bases
ATGGCCAAGGGTCGGGGCAGGATTGAGCCGAACTTCGACGTGCCCGAGGGGCGCGCGCGGGATCGCGGCGAACTCGACCT
GCGCCTGACTCGGGAAGACCGGCCGGGAGCAGGAGACGGCGTGGCGAAGCGATCGGGCGGCGGAGCGCGGCAGCCGAACA
AGGCGCCGGCGCGGACCACGGGGCGAGCGAAGGCCTCGGGTCGCGGACGACGCCGGCGTTCCTGGCTCGGCCGCATCGTC
TACGGCACCGTCGTCCTCGGCCTCTGGGGTGTGATCGGGCTCGCCGGCCTCATCGCCTACCACGCGTCGCAACTGCCGCC
GATCGACCAGCTCGCGGTGCCGAAGCGGCCGCCCAACATCGCCATCCTGGCGAGCGACGGCTCGCTGCTCGCCAATCGCG
GCGAGACCGGCGGCCGTGTGGTGTCGATCAAGGAGCTGCCGCCCTACCTGCCGCGCGCCTTCGTGGCGATCGAGGATCGG
CGCTTCTACCAGCATTTCGGCGTGGATCCCGTCGGCATCGTCCGGGCCATCGGCCAGAACCTGACCCGTCGCGGCGTGGC
GCAGGGCGGCTCGACCCTGACCCAGCAGCTCGCCAAGAACCTGTTCCTGACCCCGGAGCGCTCGGCCTCGCGCAAGATCC
AGGAGGCGATCCTCGCGCTCTGGCTCGAGCACAAGTACACCAAGGACGAGATCCTCGAACTCTACCTGAACCGCGTCTAT
TTCGGCGCCGGCGCCTACGGCGTCGAGGCGGCGGCGCAGCGCTATTTCGGCAAGCCCGCCAAGGAGGTGAGCCTCGCGCA
GGCCGCGATGCTCGGCGGGCTCGTGCAGGCGCCCTCGCGCCTCGCCCCGAACCGCAACCTGCCGGCCGCCCAGGCCCGCG
CCGCCCAGGTCCTCGCCGCCATGCAGGAGCTGGGCTTCGCGTCGGCGCAGGACGTCAAGGTTGCGCTCGCCCAGCCGGCG
AAGCCCGCCAACGCCCGCGGCGGCGGGTCGGCCAACTACGTCGCCGACCTCGTGATGGACGTGCTCGACGATTACGTCGG
CAAGTTCGACACCGACATCACGGTCCAGACCACCGTCGATCCCAGCCTCCAGGCGGCGGCCGAGAAGGCGCTGACCGACG
AGCTCAACGCGAAGGGGACGCGCTACAACGTCAGCCAGGGCGCCCTGGTCTCGATGCGGCCCGACGGCGCCATCCGCGCC
CTGATCGGCGGCCGCGACTACGCGCAGAGCCAGTTCAACCGGGCCACGACCGCCAAGCGTCAGCCCGGCTCCTCGTTCAA
GCCGTTCGTCTACCTCGCGGCCGTGGAGCACGGCGCCACGCCCGACACGGTCCGGGAGGACGCGCCGATCCGGATCGGCA
ACTGGGCGCCGGAGAACTACTCCCACCGCTATGAGGGCCCCGTCACGCTGCGGACGGCGCTCGCCCAGTCGCTCAACACG
GTCGCGGTGCGCCTCGGCCAGGAGGTCGGTCCGAAGACCGTGGTCCAGACCGCCCAGCGCCTCGGCATCACCTCGCCGCT
CCAGGCCAACGGGTCCATCGCCCTCGGTACGTCCGAGGTCACGCTCCTGGAGATGGTCGGCGCCTACGGGGCCTTCGCCA
ACGGCGGCATCGGCGTGATCCCGTACGTCGTCACCGCCGTGAAGAGCCAGGACGGGAAGGTGCTCTACAAGCGCTCCGAC
AACGGTCTGGGCCGGGTCATCGACGCCAACGCCGACGGCATGATGAACGCCATGATGCACGAGACCTTCGTGAGCGGCAC
CGGCAAGAAGGCCGACATCCCCGGCTGGGACCTCGCGGGGAAGAGCGGCACCACGCAGGATTACCGCGACGCGTGGTTCA
TGGGCTTCTCGGGCAGCCTCGTGACCGGGGTCTGGCTCGGCAACGACGACGGCGAGCTCACCAAGAAGGTCACCGGCGGC
AACCTGCCGGCGGAGATCTGGAAGACCTACATGACCCAGGCGCTGAAGGGGCAGACGCCGGTCCCGCTCCCGGGCCTGAA
TCGCTGGCGCCGGCAGCCGGAGACGACCGCCTCGGTGGCGAGCGCCGCCCCGGCTGGCCCGGGCGGGCTCCTCGGTCAGA
TCCTCGGCGAGCCGGAGGCGCCGGCGCCGCGCCAGGCCGCGCCGGCACGGCGCGCCCAGAAGGATGACCGCAACTTCATC
GAGAAGCTGTTCGGCATCGGGGACTGA

Upstream 100 bases:

>100_bases
TCCGGACGGGGCGCGAAACCGCCGCATTTGGCGTGCGGAGACCGTACTGCGGTGCGCCGGACAGACGCGCGTCGTCGCGG
AACATTCGAAGACGGGATGC

Downstream 100 bases:

>100_bases
CGCGCCGCGCCTGGCCAGGATCGCGCAAGGGCGCGCGTCGCGGGCGGGTGGCCGCCCGCGACGCGAGCCGTCCTGTATCG
ACCCGACGACGGCGGGGTTC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MAKGRGRIEPNFDVPEGRARDRGELDLRLTREDRPGAGDGVAKRSGGGARQPNKAPARTTGRAKASGRGRRRRSWLGRIV
YGTVVLGLWGVIGLAGLIAYHASQLPPIDQLAVPKRPPNIAILASDGSLLANRGETGGRVVSIKELPPYLPRAFVAIEDR
RFYQHFGVDPVGIVRAIGQNLTRRGVAQGGSTLTQQLAKNLFLTPERSASRKIQEAILALWLEHKYTKDEILELYLNRVY
FGAGAYGVEAAAQRYFGKPAKEVSLAQAAMLGGLVQAPSRLAPNRNLPAAQARAAQVLAAMQELGFASAQDVKVALAQPA
KPANARGGGSANYVADLVMDVLDDYVGKFDTDITVQTTVDPSLQAAAEKALTDELNAKGTRYNVSQGALVSMRPDGAIRA
LIGGRDYAQSQFNRATTAKRQPGSSFKPFVYLAAVEHGATPDTVREDAPIRIGNWAPENYSHRYEGPVTLRTALAQSLNT
VAVRLGQEVGPKTVVQTAQRLGITSPLQANGSIALGTSEVTLLEMVGAYGAFANGGIGVIPYVVTAVKSQDGKVLYKRSD
NGLGRVIDANADGMMNAMMHETFVSGTGKKADIPGWDLAGKSGTTQDYRDAWFMGFSGSLVTGVWLGNDDGELTKKVTGG
NLPAEIWKTYMTQALKGQTPVPLPGLNRWRRQPETTASVASAAPAGPGGLLGQILGEPEAPAPRQAAPARRAQKDDRNFI
EKLFGIGD

Sequences:

>Translated_728_residues
MAKGRGRIEPNFDVPEGRARDRGELDLRLTREDRPGAGDGVAKRSGGGARQPNKAPARTTGRAKASGRGRRRRSWLGRIV
YGTVVLGLWGVIGLAGLIAYHASQLPPIDQLAVPKRPPNIAILASDGSLLANRGETGGRVVSIKELPPYLPRAFVAIEDR
RFYQHFGVDPVGIVRAIGQNLTRRGVAQGGSTLTQQLAKNLFLTPERSASRKIQEAILALWLEHKYTKDEILELYLNRVY
FGAGAYGVEAAAQRYFGKPAKEVSLAQAAMLGGLVQAPSRLAPNRNLPAAQARAAQVLAAMQELGFASAQDVKVALAQPA
KPANARGGGSANYVADLVMDVLDDYVGKFDTDITVQTTVDPSLQAAAEKALTDELNAKGTRYNVSQGALVSMRPDGAIRA
LIGGRDYAQSQFNRATTAKRQPGSSFKPFVYLAAVEHGATPDTVREDAPIRIGNWAPENYSHRYEGPVTLRTALAQSLNT
VAVRLGQEVGPKTVVQTAQRLGITSPLQANGSIALGTSEVTLLEMVGAYGAFANGGIGVIPYVVTAVKSQDGKVLYKRSD
NGLGRVIDANADGMMNAMMHETFVSGTGKKADIPGWDLAGKSGTTQDYRDAWFMGFSGSLVTGVWLGNDDGELTKKVTGG
NLPAEIWKTYMTQALKGQTPVPLPGLNRWRRQPETTASVASAAPAGPGGLLGQILGEPEAPAPRQAAPARRAQKDDRNFI
EKLFGIGD
>Mature_727_residues
AKGRGRIEPNFDVPEGRARDRGELDLRLTREDRPGAGDGVAKRSGGGARQPNKAPARTTGRAKASGRGRRRRSWLGRIVY
GTVVLGLWGVIGLAGLIAYHASQLPPIDQLAVPKRPPNIAILASDGSLLANRGETGGRVVSIKELPPYLPRAFVAIEDRR
FYQHFGVDPVGIVRAIGQNLTRRGVAQGGSTLTQQLAKNLFLTPERSASRKIQEAILALWLEHKYTKDEILELYLNRVYF
GAGAYGVEAAAQRYFGKPAKEVSLAQAAMLGGLVQAPSRLAPNRNLPAAQARAAQVLAAMQELGFASAQDVKVALAQPAK
PANARGGGSANYVADLVMDVLDDYVGKFDTDITVQTTVDPSLQAAAEKALTDELNAKGTRYNVSQGALVSMRPDGAIRAL
IGGRDYAQSQFNRATTAKRQPGSSFKPFVYLAAVEHGATPDTVREDAPIRIGNWAPENYSHRYEGPVTLRTALAQSLNTV
AVRLGQEVGPKTVVQTAQRLGITSPLQANGSIALGTSEVTLLEMVGAYGAFANGGIGVIPYVVTAVKSQDGKVLYKRSDN
GLGRVIDANADGMMNAMMHETFVSGTGKKADIPGWDLAGKSGTTQDYRDAWFMGFSGSLVTGVWLGNDDGELTKKVTGGN
LPAEIWKTYMTQALKGQTPVPLPGLNRWRRQPETTASVASAAPAGPGGLLGQILGEPEAPAPRQAAPARRAQKDDRNFIE
KLFGIGD

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=542, Percent_Identity=35.7933579335793, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI1788867, Length=553, Percent_Identity=32.368896925859, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082258, Length=297, Percent_Identity=39.0572390572391, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=40.6060606060606, Blast_Score=120, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 77597; Mature: 77466

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGRGRIEPNFDVPEGRARDRGELDLRLTREDRPGAGDGVAKRSGGGARQPNKAPARTT
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GRAKASGRGRRRRSWLGRIVYGTVVLGLWGVIGLAGLIAYHASQLPPIDQLAVPKRPPNI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCE
AILASDGSLLANRGETGGRVVSIKELPPYLPRAFVAIEDRRFYQHFGVDPVGIVRAIGQN
EEEECCCCEEECCCCCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHH
LTRRGVAQGGSTLTQQLAKNLFLTPERSASRKIQEAILALWLEHKYTKDEILELYLNRVY
HHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FGAGAYGVEAAAQRYFGKPAKEVSLAQAAMLGGLVQAPSRLAPNRNLPAAQARAAQVLAA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHH
MQELGFASAQDVKVALAQPAKPANARGGGSANYVADLVMDVLDDYVGKFDTDITVQTTVD
HHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
PSLQAAAEKALTDELNAKGTRYNVSQGALVSMRPDGAIRALIGGRDYAQSQFNRATTAKR
HHHHHHHHHHHHHHHCCCCCEEECCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHCCC
QPGSSFKPFVYLAAVEHGATPDTVREDAPIRIGNWAPENYSHRYEGPVTLRTALAQSLNT
CCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
VAVRLGQEVGPKTVVQTAQRLGITSPLQANGSIALGTSEVTLLEMVGAYGAFANGGIGVI
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCHHH
PYVVTAVKSQDGKVLYKRSDNGLGRVIDANADGMMNAMMHETFVSGTGKKADIPGWDLAG
HHHHHHHCCCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KSGTTQDYRDAWFMGFSGSLVTGVWLGNDDGELTKKVTGGNLPAEIWKTYMTQALKGQTP
CCCCCCHHHHHEEECCCCCEEEEEEECCCCCHHHHEECCCCCCHHHHHHHHHHHHCCCCC
VPLPGLNRWRRQPETTASVASAAPAGPGGLLGQILGEPEAPAPRQAAPARRAQKDDRNFI
CCCCCCHHHHCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
EKLFGIGD
HHHHCCCC
>Mature Secondary Structure 
AKGRGRIEPNFDVPEGRARDRGELDLRLTREDRPGAGDGVAKRSGGGARQPNKAPARTT
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GRAKASGRGRRRRSWLGRIVYGTVVLGLWGVIGLAGLIAYHASQLPPIDQLAVPKRPPNI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCE
AILASDGSLLANRGETGGRVVSIKELPPYLPRAFVAIEDRRFYQHFGVDPVGIVRAIGQN
EEEECCCCEEECCCCCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHH
LTRRGVAQGGSTLTQQLAKNLFLTPERSASRKIQEAILALWLEHKYTKDEILELYLNRVY
HHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FGAGAYGVEAAAQRYFGKPAKEVSLAQAAMLGGLVQAPSRLAPNRNLPAAQARAAQVLAA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHH
MQELGFASAQDVKVALAQPAKPANARGGGSANYVADLVMDVLDDYVGKFDTDITVQTTVD
HHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
PSLQAAAEKALTDELNAKGTRYNVSQGALVSMRPDGAIRALIGGRDYAQSQFNRATTAKR
HHHHHHHHHHHHHHHCCCCCEEECCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHCCC
QPGSSFKPFVYLAAVEHGATPDTVREDAPIRIGNWAPENYSHRYEGPVTLRTALAQSLNT
CCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
VAVRLGQEVGPKTVVQTAQRLGITSPLQANGSIALGTSEVTLLEMVGAYGAFANGGIGVI
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCHHH
PYVVTAVKSQDGKVLYKRSDNGLGRVIDANADGMMNAMMHETFVSGTGKKADIPGWDLAG
HHHHHHHCCCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KSGTTQDYRDAWFMGFSGSLVTGVWLGNDDGELTKKVTGGNLPAEIWKTYMTQALKGQTP
CCCCCCHHHHHEEECCCCCEEEEEEECCCCCHHHHEECCCCCCHHHHHHHHHHHHCCCCC
VPLPGLNRWRRQPETTASVASAAPAGPGGLLGQILGEPEAPAPRQAAPARRAQKDDRNFI
CCCCCCHHHHCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
EKLFGIGD
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]