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The map label for this gene is garD [H]

Identifier: 170749464

GI number: 170749464

Start: 3231251

End: 3232774

Strand: Reverse

Name: garD [H]

Synonym: Mrad2831_3059

Alternate gene names: 170749464

Gene position: 3232774-3231251 (Counterclockwise)

Preceding gene: 170749465

Following gene: 170749462

Centisome position: 53.19

GC content: 69.49

Gene sequence:

>1524_bases
ATGTCGACCTACCCCGTGATCACCCTTCGGCCGGCCGATGGTGTCGCCGTGGCCCGCGGCGCCATCGAGCCGGGAATGCC
GGTCGCCGACGGCGTCGCGGCGGCGGAGCGGATCCCGGCCGGCCACAAGGTCGCGGTCCGGGCCCATCGCGCTGGCGAGC
CGGTGACCAAGTACGGACAGATCATCGGCTTCGCCAAGGACGACATCGCGGCCGGCCGCCACGTCCACGTCCACAATCTC
GGGATGGGCGAGTTCGCCCGGGACTACGCGTTCGGGGTCGACGCGAGGCCGACCCATCTGGTGACGCCGCCCGCGACCTT
CCAGGGCATCCGCCGCCCCGACGGGCGCGTGGCGACGCGCAACTACGTGGGCATCCTCACCTCGGTGAATTGCAGCGCGC
ACGTCGCCGACCTGATCGCCGACGCGTTCCGGCGGCACCCGATCCTCGGCCTCGACCCGCTCGCTCCCTATCCCAACGTC
GACGGTGTCGTGGCGCTGACCCACAAGACCGGCTGCGGCATGGCGATGGGCGAGCCGCTGCGGCTCCTGCGCCGGACGCT
CGCGGGCTACGCGCGGCACGTCAACTTCTCCCACATCGTGATGATCGGGCTCGGCTGCGAGGTGAACCAGATCGGCGCCT
TCTTGGAGGAGCAGGGGCTCGGCGACCGGATCCGCAGCATGAACATCCAGAGCCTCGGCGGCACACGGAAGACCGTCGAG
GCCGGCATCGCGTTCGTGAAGGACATCCTGGCCGAGGCGAACGTGGTCCGCCGCGAGCCGGTGCCGGCGAGCGAGCTGAT
CGTCGCCCTGCAATGCGGCGGCTCGGACGGCTATTCCGGAGTCTCGGCCAACCCGGCTCTGGGAATCGCCAGCGACCTCG
TGGTGCGGAACGGCGGCACGGTGATCCTGTCGGAGACCACCGAGACCTACGGCGCCGAGCACCTATTCACCCGCCGCGCC
GTCAGTCCGGAAGTCGGCCAGAAGCTCGTCGACCTGATGCACTGGTGGGAGGAGTACACCCGCAAGGAGGGCTCGGAGAT
CGACGCCAACCCGTCGCCGGGCAACAAGGCCGGCGGCCTGACGACGATCCTGGAGAAGTCGCTCGGCGCCATGGCGAAGG
CCGGCAGCACCAACCTCGTGGACGTGCTGCGCTACGCCGATCCCGTCACCGCCAAGGGCTTCGTCTTCATGGACACGCCC
GGCTACGACCCGGTCTCGGCGACCGGGCAGGTCGCGGGCGGGGCGAACCTCGTCTGCTTCACCACGGGACGCGGCTCCGT
ATTCGGCTGCAAGCCGTCGCCCTCGATCAAAATCGCCACCAACTCCGCCATGTACCGGCGGCTCGAGGAGGACATGGACG
TCAATTGCGGCACGATCCTCGACGGTGAAGAGAGCGTGGAGCAGGCTGGGCGCCGGATCTTCGACCTGATCCTGCGGGTC
GCCGGCGGCGAGCGGACGAAGAGCGAGCAGTTCGATTTCGGCTCGTCGGAATTCGCGCCCTGGCAGATCGGCGCGACCGT
GTGA

Upstream 100 bases:

>100_bases
AGATCGCGCCGCTGCCGGTCCGCGACGCCCGCGCCCCGGCTCTGCGCCCCGAGGCGGCGCGGTGACGGCGAGCGGCCCGA
TCGAATCCGAGGAGAGACCG

Downstream 100 bases:

>100_bases
GGGGCCCGGGCGTGCGCTCCGCCGTCGTTGCGAGCGCAGCGACGGTCAGGGCGATCACCGAGCAGGCCGCATCTCAAAGG
TCCGTTCGCACCACGCCCGG

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSTYPVITLRPADGVAVARGAIEPGMPVADGVAAAERIPAGHKVAVRAHRAGEPVTKYGQIIGFAKDDIAAGRHVHVHNL
GMGEFARDYAFGVDARPTHLVTPPATFQGIRRPDGRVATRNYVGILTSVNCSAHVADLIADAFRRHPILGLDPLAPYPNV
DGVVALTHKTGCGMAMGEPLRLLRRTLAGYARHVNFSHIVMIGLGCEVNQIGAFLEEQGLGDRIRSMNIQSLGGTRKTVE
AGIAFVKDILAEANVVRREPVPASELIVALQCGGSDGYSGVSANPALGIASDLVVRNGGTVILSETTETYGAEHLFTRRA
VSPEVGQKLVDLMHWWEEYTRKEGSEIDANPSPGNKAGGLTTILEKSLGAMAKAGSTNLVDVLRYADPVTAKGFVFMDTP
GYDPVSATGQVAGGANLVCFTTGRGSVFGCKPSPSIKIATNSAMYRRLEEDMDVNCGTILDGEESVEQAGRRIFDLILRV
AGGERTKSEQFDFGSSEFAPWQIGATV

Sequences:

>Translated_507_residues
MSTYPVITLRPADGVAVARGAIEPGMPVADGVAAAERIPAGHKVAVRAHRAGEPVTKYGQIIGFAKDDIAAGRHVHVHNL
GMGEFARDYAFGVDARPTHLVTPPATFQGIRRPDGRVATRNYVGILTSVNCSAHVADLIADAFRRHPILGLDPLAPYPNV
DGVVALTHKTGCGMAMGEPLRLLRRTLAGYARHVNFSHIVMIGLGCEVNQIGAFLEEQGLGDRIRSMNIQSLGGTRKTVE
AGIAFVKDILAEANVVRREPVPASELIVALQCGGSDGYSGVSANPALGIASDLVVRNGGTVILSETTETYGAEHLFTRRA
VSPEVGQKLVDLMHWWEEYTRKEGSEIDANPSPGNKAGGLTTILEKSLGAMAKAGSTNLVDVLRYADPVTAKGFVFMDTP
GYDPVSATGQVAGGANLVCFTTGRGSVFGCKPSPSIKIATNSAMYRRLEEDMDVNCGTILDGEESVEQAGRRIFDLILRV
AGGERTKSEQFDFGSSEFAPWQIGATV
>Mature_506_residues
STYPVITLRPADGVAVARGAIEPGMPVADGVAAAERIPAGHKVAVRAHRAGEPVTKYGQIIGFAKDDIAAGRHVHVHNLG
MGEFARDYAFGVDARPTHLVTPPATFQGIRRPDGRVATRNYVGILTSVNCSAHVADLIADAFRRHPILGLDPLAPYPNVD
GVVALTHKTGCGMAMGEPLRLLRRTLAGYARHVNFSHIVMIGLGCEVNQIGAFLEEQGLGDRIRSMNIQSLGGTRKTVEA
GIAFVKDILAEANVVRREPVPASELIVALQCGGSDGYSGVSANPALGIASDLVVRNGGTVILSETTETYGAEHLFTRRAV
SPEVGQKLVDLMHWWEEYTRKEGSEIDANPSPGNKAGGLTTILEKSLGAMAKAGSTNLVDVLRYADPVTAKGFVFMDTPG
YDPVSATGQVAGGANLVCFTTGRGSVFGCKPSPSIKIATNSAMYRRLEEDMDVNCGTILDGEESVEQAGRRIFDLILRVA
GGERTKSEQFDFGSSEFAPWQIGATV

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=504, Percent_Identity=39.8809523809524, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI1789475, Length=507, Percent_Identity=35.8974358974359, Blast_Score=269, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 53817; Mature: 53686

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTYPVITLRPADGVAVARGAIEPGMPVADGVAAAERIPAGHKVAVRAHRAGEPVTKYGQ
CCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
IIGFAKDDIAAGRHVHVHNLGMGEFARDYAFGVDARPTHLVTPPATFQGIRRPDGRVATR
HEEECCHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEECCCHHHHCCCCCCCCEEEC
NYVGILTSVNCSAHVADLIADAFRRHPILGLDPLAPYPNVDGVVALTHKTGCGMAMGEPL
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHH
RLLRRTLAGYARHVNFSHIVMIGLGCEVNQIGAFLEEQGLGDRIRSMNIQSLGGTRKTVE
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCHHHCCCCHHHHH
AGIAFVKDILAEANVVRREPVPASELIVALQCGGSDGYSGVSANPALGIASDLVVRNGGT
HHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCHHHEEEECCCE
VILSETTETYGAEHLFTRRAVSPEVGQKLVDLMHWWEEYTRKEGSEIDANPSPGNKAGGL
EEEECCCHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
TTILEKSLGAMAKAGSTNLVDVLRYADPVTAKGFVFMDTPGYDPVSATGQVAGGANLVCF
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEE
TTGRGSVFGCKPSPSIKIATNSAMYRRLEEDMDVNCGTILDGEESVEQAGRRIFDLILRV
ECCCCCEEECCCCCCEEEEECHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHH
AGGERTKSEQFDFGSSEFAPWQIGATV
CCCCCCCCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure 
STYPVITLRPADGVAVARGAIEPGMPVADGVAAAERIPAGHKVAVRAHRAGEPVTKYGQ
CCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
IIGFAKDDIAAGRHVHVHNLGMGEFARDYAFGVDARPTHLVTPPATFQGIRRPDGRVATR
HEEECCHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEECCCHHHHCCCCCCCCEEEC
NYVGILTSVNCSAHVADLIADAFRRHPILGLDPLAPYPNVDGVVALTHKTGCGMAMGEPL
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHH
RLLRRTLAGYARHVNFSHIVMIGLGCEVNQIGAFLEEQGLGDRIRSMNIQSLGGTRKTVE
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCHHHCCCCHHHHH
AGIAFVKDILAEANVVRREPVPASELIVALQCGGSDGYSGVSANPALGIASDLVVRNGGT
HHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCHHHEEEECCCE
VILSETTETYGAEHLFTRRAVSPEVGQKLVDLMHWWEEYTRKEGSEIDANPSPGNKAGGL
EEEECCCHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
TTILEKSLGAMAKAGSTNLVDVLRYADPVTAKGFVFMDTPGYDPVSATGQVAGGANLVCF
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEE
TTGRGSVFGCKPSPSIKIATNSAMYRRLEEDMDVNCGTILDGEESVEQAGRRIFDLILRV
ECCCCCEEECCCCCCEEEEECHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHH
AGGERTKSEQFDFGSSEFAPWQIGATV
CCCCCCCCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]