Click here to switch to the map view.

The map label for this gene is ptsN [H]

Identifier: 170749391

GI number: 170749391

Start: 3140019

End: 3140486

Strand: Reverse

Name: ptsN [H]

Synonym: Mrad2831_2984

Alternate gene names: 170749391

Gene position: 3140486-3140019 (Counterclockwise)

Preceding gene: 170749392

Following gene: 170749390

Centisome position: 51.67

GC content: 74.15

Gene sequence:

>468_bases
ATGCCAGTTCTGGAATTCCTCGACCCCGAGTCGGTCGTGCCGGCCCTGCGCGCCCGCGCCAAGAAGCAGGTGCTGCAGGA
TCTCGCCGCGCAGGCGGCGCGGCGCCTGCCCGCCCTCGGCGAGCGCCCCGTCTTCGACACCCTTCTCCAGCGCGAGCGGC
TCGGCTCGACCGGCATCGGCGACGGGGTGGCGATCCCGCACGGCAAGCTGCCCGGCCTGGACCGTCTCTTCGGGCTGTTC
GCGCGCTTCGACCGCCCGGTGGATTTCGAGGCCCTGGATGGCCAGCCCGTGGACATCGCCTTCCTGCTGCTCGCGCCCGA
GGGTGCCGGCGCCGACCACCTGAAGGCCCTGGCGCAGGTGGCCCGTATCCTGCGCGAGCCGGGCATGCTCGCGCATATCC
GCGCCGCCCGGGACGCCGGGGCGCTCTACGCGCTCCTGACGCGCTCGACGGCACCGCAGGCCGCCTGA

Upstream 100 bases:

>100_bases
CGCCCATTGAATCCAGCGGGGGCGCGGCCGGAACGGTCGTCGACGCCCCTGCGACGAGGCCCGCTTCCTCGCGAGCGGGC
GACGGGGCAACGGTCGTTCG

Downstream 100 bases:

>100_bases
TGATCCCGAGCTTCCGCATCGCCGCCCTGATCCTCGCGGCCGGCGTCTCCGGCGCCGCCGCCCAGGGCGAGACCACCTGC
GCCCGCGACGTCCTGGTGGC

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MPVLEFLDPESVVPALRARAKKQVLQDLAAQAARRLPALGERPVFDTLLQRERLGSTGIGDGVAIPHGKLPGLDRLFGLF
ARFDRPVDFEALDGQPVDIAFLLLAPEGAGADHLKALAQVARILREPGMLAHIRAARDAGALYALLTRSTAPQAA

Sequences:

>Translated_155_residues
MPVLEFLDPESVVPALRARAKKQVLQDLAAQAARRLPALGERPVFDTLLQRERLGSTGIGDGVAIPHGKLPGLDRLFGLF
ARFDRPVDFEALDGQPVDIAFLLLAPEGAGADHLKALAQVARILREPGMLAHIRAARDAGALYALLTRSTAPQAA
>Mature_154_residues
PVLEFLDPESVVPALRARAKKQVLQDLAAQAARRLPALGERPVFDTLLQRERLGSTGIGDGVAIPHGKLPGLDRLFGLFA
RFDRPVDFEALDGQPVDIAFLLLAPEGAGADHLKALAQVARILREPGMLAHIRAARDAGALYALLTRSTAPQAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=142, Percent_Identity=38.7323943661972, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI2367327, Length=134, Percent_Identity=29.8507462686567, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 16586; Mature: 16455

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLEFLDPESVVPALRARAKKQVLQDLAAQAARRLPALGERPVFDTLLQRERLGSTGIG
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
DGVAIPHGKLPGLDRLFGLFARFDRPVDFEALDGQPVDIAFLLLAPEGAGADHLKALAQV
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHH
ARILREPGMLAHIRAARDAGALYALLTRSTAPQAA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PVLEFLDPESVVPALRARAKKQVLQDLAAQAARRLPALGERPVFDTLLQRERLGSTGIG
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
DGVAIPHGKLPGLDRLFGLFARFDRPVDFEALDGQPVDIAFLLLAPEGAGADHLKALAQV
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHH
ARILREPGMLAHIRAARDAGALYALLTRSTAPQAA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]