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The map label for this gene is ilvD2 [H]

Identifier: 170749331

GI number: 170749331

Start: 3082295

End: 3084025

Strand: Reverse

Name: ilvD2 [H]

Synonym: Mrad2831_2924

Alternate gene names: 170749331

Gene position: 3084025-3082295 (Counterclockwise)

Preceding gene: 170749332

Following gene: 170749330

Centisome position: 50.74

GC content: 70.88

Gene sequence:

>1731_bases
ATGGTGACGCGTTTGAACGACAAGTCGAAGCTGCCGAGCCGCCACGTCACGGAGGGGCCCGACCGCGCCCCGCACCGCTC
CTACCTCTACGCCATGGGCCTGACCCGCGAGCAGATCCACCAGCCGCTGGTGGGTGTCGCCTCGTGCTGGAACGAGGCCG
CGCCCTGCAACATCTCGCTGATGCGCCAGGCGCAGGCGGTGAAGAAGGGCGTCGCCGCCGCCAACGGGACGCCGCGCGAG
TTCTGCACGATCACCGTCACCGACGGCATCGCCATGGGCCACGGCGGCATGCGCGCGTCGCTGCCGTCGCGGGAGGTTAT
CGCCGACTCGGTCGAGCTGACCATGCGCGGCCACGCCTACGACGCCCTCGTGGGTCTGGCGGGCTGCGACAAGTCGCTGC
CCGGCATGATGATGGCGATGGTCCGCCTGAACGTGCCCTCGATCTTCATCTACGGCGGCTCGATCCTGCCGGGCTCGTTC
CGTGGCAAGCCCGTGACCGTTCAGGACCTGTTCGAGGCGGTCGGCAAGGTGGCCGTCGGCGACATGAGCCTCGAGGATCT
CGACGAGCTGGAGCAGGTCGCCTGCCCGTCGGCCGGCGCCTGCGGCGCGCAGTTCACCGCCAACACCATGGCGACCGTCT
CCGAGGCGATCGGCCTGGCGCTGCCGTACTCGGCCGGCGCCCCGGCCCCCTACGAGATCCGCGACAAGTTCTGCGCGACC
GCCGGCGAGAAGGTGATGGAGCTTCTGGAGAAGCGCATCCGCCCGCGCGACATCGTCACCCGCAAGGCCCTGGAGAACGC
CGCCACGGTGGTGGCGGCCTCGGGCGGCTCGACCAACGCGGCGCTGCACCTGCCGGCGATCGCGCACGAGTGCGGCATCG
AGTTCACGCTGTTCGACGTGGCCGAGATCTTCCGGCGCACGCCCTACATCGCCGACCTGAAGCCCGGCGGGCGCTATGTC
GCCAAGGACATGTTCGAGGTCGGCGGCATCCCGCTGCTGATGAAGACGCTCCTCGACCACGGCTTCATGCACGGCGACTG
CATGACGGTCACCGGCCGCACCATCGCGGAGAACATGGACCGGGTGACCTGGAACCCGGACCAGGACGTGGTCTACCCGG
CCAACCGCCCGATCACCCCGACCGGCGGCGTCGTCGGCCTCAAGGGCAACCTCGCCCCCGAGGGCGCGATCGTGAAGGTC
GCCGGCATCCCGGCCGAGAAGCAGGTCTTCACCGGCCCGGCCCGCGTGTTCGACGGCGAGGAGGCCTGCTTCGCGGCCGT
GCAGGCGCGCAGCTACAAGGAAGGCGACGTCCTCGTGATCCGCTACGAGGGTCCGCGGGGCGGCCCCGGCATGCGCGAGA
TGCTGTCGACCACCGCGGCCCTCTACGGCCAGGGCATGGGCGACAAGGTCGCGCTGATCACCGACGGGCGCTTCTCGGGC
GCGACCCGGGGCTTCTGCGTCGGCCATGTCGGCCCCGAGGCCGCCGTGGGCGGCCCGATCGGGCTGCTCCGCGACGGCGA
CGTGATCACCCTCGACGCGATCCAGGGCACGCTCAGCGTCGCCCTGTCCGACGCGGAACTGGCCGAGCGCCGCAAGGCCT
GGACCGCGCGGCCCAACACCGCGACCTCCGGCTACCTCTGGAAATACGCGCAGGGCGTCGGCCCGGCCCTGTACGGCGCC
GTCACCCATCCGGGAGGTGCCAAGGAGACGCAGAGCTATGCGGACGTCTAG

Upstream 100 bases:

>100_bases
TTCCCGAGTACGCGGACGGATTTCCCGGCGCTTCGCGAACGGCGACGCGCCGCGGTTCAACGCACCCTCGGGATCGGCTG
CGAAGGCTTGGTCTGAAGTC

Downstream 100 bases:

>100_bases
GACTGACCTCAGCCGGCGCCGGGCGCCGGCCGGGGCCGATCCCGGTCGGTTCCGGTGCGTCGGCGGGCTCGCGGTCGCGG
CCTGGGCGCTCGCCCTGCTC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 2 [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MVTRLNDKSKLPSRHVTEGPDRAPHRSYLYAMGLTREQIHQPLVGVASCWNEAAPCNISLMRQAQAVKKGVAAANGTPRE
FCTITVTDGIAMGHGGMRASLPSREVIADSVELTMRGHAYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGSF
RGKPVTVQDLFEAVGKVAVGDMSLEDLDELEQVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPAPYEIRDKFCAT
AGEKVMELLEKRIRPRDIVTRKALENAATVVAASGGSTNAALHLPAIAHECGIEFTLFDVAEIFRRTPYIADLKPGGRYV
AKDMFEVGGIPLLMKTLLDHGFMHGDCMTVTGRTIAENMDRVTWNPDQDVVYPANRPITPTGGVVGLKGNLAPEGAIVKV
AGIPAEKQVFTGPARVFDGEEACFAAVQARSYKEGDVLVIRYEGPRGGPGMREMLSTTAALYGQGMGDKVALITDGRFSG
ATRGFCVGHVGPEAAVGGPIGLLRDGDVITLDAIQGTLSVALSDAELAERRKAWTARPNTATSGYLWKYAQGVGPALYGA
VTHPGGAKETQSYADV

Sequences:

>Translated_576_residues
MVTRLNDKSKLPSRHVTEGPDRAPHRSYLYAMGLTREQIHQPLVGVASCWNEAAPCNISLMRQAQAVKKGVAAANGTPRE
FCTITVTDGIAMGHGGMRASLPSREVIADSVELTMRGHAYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGSF
RGKPVTVQDLFEAVGKVAVGDMSLEDLDELEQVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPAPYEIRDKFCAT
AGEKVMELLEKRIRPRDIVTRKALENAATVVAASGGSTNAALHLPAIAHECGIEFTLFDVAEIFRRTPYIADLKPGGRYV
AKDMFEVGGIPLLMKTLLDHGFMHGDCMTVTGRTIAENMDRVTWNPDQDVVYPANRPITPTGGVVGLKGNLAPEGAIVKV
AGIPAEKQVFTGPARVFDGEEACFAAVQARSYKEGDVLVIRYEGPRGGPGMREMLSTTAALYGQGMGDKVALITDGRFSG
ATRGFCVGHVGPEAAVGGPIGLLRDGDVITLDAIQGTLSVALSDAELAERRKAWTARPNTATSGYLWKYAQGVGPALYGA
VTHPGGAKETQSYADV
>Mature_576_residues
MVTRLNDKSKLPSRHVTEGPDRAPHRSYLYAMGLTREQIHQPLVGVASCWNEAAPCNISLMRQAQAVKKGVAAANGTPRE
FCTITVTDGIAMGHGGMRASLPSREVIADSVELTMRGHAYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGSF
RGKPVTVQDLFEAVGKVAVGDMSLEDLDELEQVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPAPYEIRDKFCAT
AGEKVMELLEKRIRPRDIVTRKALENAATVVAASGGSTNAALHLPAIAHECGIEFTLFDVAEIFRRTPYIADLKPGGRYV
AKDMFEVGGIPLLMKTLLDHGFMHGDCMTVTGRTIAENMDRVTWNPDQDVVYPANRPITPTGGVVGLKGNLAPEGAIVKV
AGIPAEKQVFTGPARVFDGEEACFAAVQARSYKEGDVLVIRYEGPRGGPGMREMLSTTAALYGQGMGDKVALITDGRFSG
ATRGFCVGHVGPEAAVGGPIGLLRDGDVITLDAIQGTLSVALSDAELAERRKAWTARPNTATSGYLWKYAQGVGPALYGA
VTHPGGAKETQSYADV

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=577, Percent_Identity=37.9549393414211, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI2367371, Length=485, Percent_Identity=36.4948453608247, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1786464, Length=495, Percent_Identity=37.3737373737374, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1788157, Length=508, Percent_Identity=28.9370078740157, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322476, Length=567, Percent_Identity=43.5626102292769, Blast_Score=460, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 60858; Mature: 60858

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTRLNDKSKLPSRHVTEGPDRAPHRSYLYAMGLTREQIHQPLVGVASCWNEAAPCNISL
CCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHH
MRQAQAVKKGVAAANGTPREFCTITVTDGIAMGHGGMRASLPSREVIADSVELTMRGHAY
HHHHHHHHHHHHHCCCCCCEEEEEEEECCEEECCCCCCCCCCCHHHHHCCEEEEECCCHH
DALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGSFRGKPVTVQDLFEAVGKVAVG
HHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCCEEHHHHHHHHCCEEEC
DMSLEDLDELEQVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPAPYEIRDKFCAT
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCEECCCCCCCCCCHHHHHHHHHH
AGEKVMELLEKRIRPRDIVTRKALENAATVVAASGGSTNAALHLPAIAHECGIEFTLFDV
HHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCCCCCCEEECCHHHHHHCCEEEHHHH
AEIFRRTPYIADLKPGGRYVAKDMFEVGGIPLLMKTLLDHGFMHGDCMTVTGRTIAENMD
HHHHHHCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCHHHHHCCC
RVTWNPDQDVVYPANRPITPTGGVVGLKGNLAPEGAIVKVAGIPAEKQVFTGPARVFDGE
CCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCEECCCHHEECCH
EACFAAVQARSYKEGDVLVIRYEGPRGGPGMREMLSTTAALYGQGMGDKVALITDGRFSG
HHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
ATRGFCVGHVGPEAAVGGPIGLLRDGDVITLDAIQGTLSVALSDAELAERRKAWTARPNT
CCCCEEEECCCCCCCCCCCEEEECCCCEEEEEECCCEEHEEECHHHHHHHHHHCCCCCCC
ATSGYLWKYAQGVGPALYGAVTHPGGAKETQSYADV
CCCCHHHHHHHCCCHHHHCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MVTRLNDKSKLPSRHVTEGPDRAPHRSYLYAMGLTREQIHQPLVGVASCWNEAAPCNISL
CCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHH
MRQAQAVKKGVAAANGTPREFCTITVTDGIAMGHGGMRASLPSREVIADSVELTMRGHAY
HHHHHHHHHHHHHCCCCCCEEEEEEEECCEEECCCCCCCCCCCHHHHHCCEEEEECCCHH
DALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGSFRGKPVTVQDLFEAVGKVAVG
HHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCCEEHHHHHHHHCCEEEC
DMSLEDLDELEQVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPAPYEIRDKFCAT
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCEECCCCCCCCCCHHHHHHHHHH
AGEKVMELLEKRIRPRDIVTRKALENAATVVAASGGSTNAALHLPAIAHECGIEFTLFDV
HHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCCCCCCEEECCHHHHHHCCEEEHHHH
AEIFRRTPYIADLKPGGRYVAKDMFEVGGIPLLMKTLLDHGFMHGDCMTVTGRTIAENMD
HHHHHHCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCHHHHHCCC
RVTWNPDQDVVYPANRPITPTGGVVGLKGNLAPEGAIVKVAGIPAEKQVFTGPARVFDGE
CCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCEECCCHHEECCH
EACFAAVQARSYKEGDVLVIRYEGPRGGPGMREMLSTTAALYGQGMGDKVALITDGRFSG
HHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
ATRGFCVGHVGPEAAVGGPIGLLRDGDVITLDAIQGTLSVALSDAELAERRKAWTARPNT
CCCCEEEECCCCCCCCCCCEEEECCCCEEEEEECCCEEHEEECHHHHHHHHHHCCCCCCC
ATSGYLWKYAQGVGPALYGAVTHPGGAKETQSYADV
CCCCHHHHHHHCCCHHHHCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]