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The map label for this gene is livK [C]

Identifier: 170749308

GI number: 170749308

Start: 3055557

End: 3056816

Strand: Reverse

Name: livK [C]

Synonym: Mrad2831_2901

Alternate gene names: 170749308

Gene position: 3056816-3055557 (Counterclockwise)

Preceding gene: 170749314

Following gene: 170749307

Centisome position: 50.29

GC content: 67.54

Gene sequence:

>1260_bases
ATGACGGACCGGTACGGGGATTCCCTTTCTCGCCGCAGCCTTCTGCGCGCCGGCGGCGCGGCGCTCCTCGCGCCGGCCCT
CGCGAGCCCGGCGCTCTCTCAGGGACAGTCGGAGGTGATCCGGATCGGCCATCTGACGCCGCGCACGGGCTTCCTCGGCC
CGCTCGGGGAGTTCGCCGTGCAGGCGGCCCAGCTCGCGGTGGAGGAGATCAACGCCGCCGGCGGCATCGCGGGCCGGAAG
GTCGAGCTGCTCTCGGAGGACAGCGTCAATCCGCAGACCGCCTCGGCCAAGGCCGAGCGCATGGTCCAGCGCGACCGCGT
GGCCTGCCTCGTCGGCGAGATCAACTCGGCCTCCGCGCTGGCGATCAGCCAGGTCGCCCAGCGGGAGAAAATCCTGTTCA
TCAACACCGGCGCCAACTCGGACGCGCTGCGCGGCTCCGACTGCAAGCGCTTCATGTTCCACGTCGAGTCGCAGAACACC
ATGATGGTGCGCGCCTGCGGGCGGGCGCTGCTCGACCGGGGCCTGATCAAGGGCCGCAAGGTCTACGCGCTCACGGCCGA
CTACGCCTTCGGCCACGATCTCCTGAAGCAGGCGAAGAAGTACTTCGCGGCCAACGACGCCACCCTCGTGGGCGAGGACC
TGATCCCCACCGAGCTGACCGACTTCTCGCCGTTCCTGCTGAAGCTCCGTCAGGCCAAGCCGGACCTCGTGATCTGCAAC
CTCGCGGGCGCGCAGATCACCAACTTCCTCAAGCAGTACAGCGAGTTCGGCCTGCCGTTCCCGGTCGCCGGATTCGGCTT
CGACACGGCGCTCGCCTGGGGCGCCGGGCCGGGCAACTTCCTCGGTACGTGGCCGGTGATCTGGCATCACCAGATCGACT
CGCCCTCGGCCAAGAAGTTCGTCGCCGCCTTCAAGGCCAAGTACAAGAAGCCGCCGGAGAACCAGGGCTGGGGCGACTAC
ACCGCGATGAAGATCGTCGCGCAATCCATGAACGAGCTGAAATCGACCGAGTCGATCAAGGTCGTGGAGCACCTCGAATC
GGGCGCCAAGTTCGACATCGCGAAGGTCCGCGAGGGCTATTTCCGCCCCTGGGATCACGAGCTGATGCAGGAGATGTTCG
CGATCACCGCCCTGCCGGCCGATCAGGTCAAGAACCCTTTCGACATCTTCACCTCGACCGGCCCGCTGCCCGGCCCCGGC
GAGAGCCTGGAAGTGCTCGCCACCACCCGCGACGAGAACGCCTGCACCTTCCCGGCGTGA

Upstream 100 bases:

>100_bases
CCGGCACTATCGCGCTAGTGATCTGTGATCACAGTTCGGGTGAGGGCCGTCAGAGCCCGCCGCAACAACCCGGCTGGAGG
ACACGCACGGGAGCGGAACG

Downstream 100 bases:

>100_bases
GGCGGCCATGCAGCTCGTCTTCATCCTGGAGCAGGTGCTCAACGGCCTGCTGATCGGCGTGCAGTACCTGCTGATCGCGC
TGGGCCTGTCGCTGATCTTC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MTDRYGDSLSRRSLLRAGGAALLAPALASPALSQGQSEVIRIGHLTPRTGFLGPLGEFAVQAAQLAVEEINAAGGIAGRK
VELLSEDSVNPQTASAKAERMVQRDRVACLVGEINSASALAISQVAQREKILFINTGANSDALRGSDCKRFMFHVESQNT
MMVRACGRALLDRGLIKGRKVYALTADYAFGHDLLKQAKKYFAANDATLVGEDLIPTELTDFSPFLLKLRQAKPDLVICN
LAGAQITNFLKQYSEFGLPFPVAGFGFDTALAWGAGPGNFLGTWPVIWHHQIDSPSAKKFVAAFKAKYKKPPENQGWGDY
TAMKIVAQSMNELKSTESIKVVEHLESGAKFDIAKVREGYFRPWDHELMQEMFAITALPADQVKNPFDIFTSTGPLPGPG
ESLEVLATTRDENACTFPA

Sequences:

>Translated_419_residues
MTDRYGDSLSRRSLLRAGGAALLAPALASPALSQGQSEVIRIGHLTPRTGFLGPLGEFAVQAAQLAVEEINAAGGIAGRK
VELLSEDSVNPQTASAKAERMVQRDRVACLVGEINSASALAISQVAQREKILFINTGANSDALRGSDCKRFMFHVESQNT
MMVRACGRALLDRGLIKGRKVYALTADYAFGHDLLKQAKKYFAANDATLVGEDLIPTELTDFSPFLLKLRQAKPDLVICN
LAGAQITNFLKQYSEFGLPFPVAGFGFDTALAWGAGPGNFLGTWPVIWHHQIDSPSAKKFVAAFKAKYKKPPENQGWGDY
TAMKIVAQSMNELKSTESIKVVEHLESGAKFDIAKVREGYFRPWDHELMQEMFAITALPADQVKNPFDIFTSTGPLPGPG
ESLEVLATTRDENACTFPA
>Mature_418_residues
TDRYGDSLSRRSLLRAGGAALLAPALASPALSQGQSEVIRIGHLTPRTGFLGPLGEFAVQAAQLAVEEINAAGGIAGRKV
ELLSEDSVNPQTASAKAERMVQRDRVACLVGEINSASALAISQVAQREKILFINTGANSDALRGSDCKRFMFHVESQNTM
MVRACGRALLDRGLIKGRKVYALTADYAFGHDLLKQAKKYFAANDATLVGEDLIPTELTDFSPFLLKLRQAKPDLVICNL
AGAQITNFLKQYSEFGLPFPVAGFGFDTALAWGAGPGNFLGTWPVIWHHQIDSPSAKKFVAAFKAKYKKPPENQGWGDYT
AMKIVAQSMNELKSTESIKVVEHLESGAKFDIAKVREGYFRPWDHELMQEMFAITALPADQVKNPFDIFTSTGPLPGPGE
SLEVLATTRDENACTFPA

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=334, Percent_Identity=23.9520958083832, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994941, Length=338, Percent_Identity=23.3727810650888, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 45469; Mature: 45338

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRYGDSLSRRSLLRAGGAALLAPALASPALSQGQSEVIRIGHLTPRTGFLGPLGEFAV
CCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCHHHHHH
QAAQLAVEEINAAGGIAGRKVELLSEDSVNPQTASAKAERMVQRDRVACLVGEINSASAL
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHH
AISQVAQREKILFINTGANSDALRGSDCKRFMFHVESQNTMMVRACGRALLDRGLIKGRK
HHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCEEHHHHHHHHHHHHHHCCCCE
VYALTADYAFGHDLLKQAKKYFAANDATLVGEDLIPTELTDFSPFLLKLRQAKPDLVICN
EEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEE
LAGAQITNFLKQYSEFGLPFPVAGFGFDTALAWGAGPGNFLGTWPVIWHHQIDSPSAKKF
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHH
VAAFKAKYKKPPENQGWGDYTAMKIVAQSMNELKSTESIKVVEHLESGAKFDIAKVREGY
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHCC
FRPWDHELMQEMFAITALPADQVKNPFDIFTSTGPLPGPGESLEVLATTRDENACTFPA
CCCCHHHHHHHHHHHHCCCHHHCCCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TDRYGDSLSRRSLLRAGGAALLAPALASPALSQGQSEVIRIGHLTPRTGFLGPLGEFAV
CCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCHHHHHH
QAAQLAVEEINAAGGIAGRKVELLSEDSVNPQTASAKAERMVQRDRVACLVGEINSASAL
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHH
AISQVAQREKILFINTGANSDALRGSDCKRFMFHVESQNTMMVRACGRALLDRGLIKGRK
HHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCEEHHHHHHHHHHHHHHCCCCE
VYALTADYAFGHDLLKQAKKYFAANDATLVGEDLIPTELTDFSPFLLKLRQAKPDLVICN
EEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEE
LAGAQITNFLKQYSEFGLPFPVAGFGFDTALAWGAGPGNFLGTWPVIWHHQIDSPSAKKF
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHH
VAAFKAKYKKPPENQGWGDYTAMKIVAQSMNELKSTESIKVVEHLESGAKFDIAKVREGY
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHCC
FRPWDHELMQEMFAITALPADQVKNPFDIFTSTGPLPGPGESLEVLATTRDENACTFPA
CCCCHHHHHHHHHHHHCCCHHHCCCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA