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The map label for this gene is potH [H]

Identifier: 170749279

GI number: 170749279

Start: 3021689

End: 3022660

Strand: Reverse

Name: potH [H]

Synonym: Mrad2831_2872

Alternate gene names: 170749279

Gene position: 3022660-3021689 (Counterclockwise)

Preceding gene: 170749280

Following gene: 170749278

Centisome position: 49.73

GC content: 71.3

Gene sequence:

>972_bases
ATGAGGACCGGTGTGCCGGACGAGGACCCCGCGGCGGGGCAACCGGGGGCCGGGTCCGCGCGCGGCCGGACCCGCGGCCG
GCTGTTCGACCGGCTGGTCCGCCTCCCGCCCTTCCTGTGGCTGGCGGCGTTCTTCCTGCTGCCCTTCGCCATCACGCTCA
AGATCAGCCTGTCGGAACCCGCCACCGCGATCCCGCCCTACCTGCCGCTCCTCGACTGGCGCGGCGGGCTCGACGGCTGG
TCGGCCTTCCTCGAAGCCCTGAACTTCGACAATTACCTGACGCTCGCCGCCGATCCCCTCTACCGCGACGCGGCCCTCGG
CTCCCTGACCTACGCGGGCGTGGCCAGCCTGATCCTCGTCGGGCTGGGGACGCCTCTGGCCTACGCCCTGGCGCGGGCGC
CGTCCCGCTGGCAGCCGCTCCTCGTGGCGCTGGTGATCGTGCCGTTCTGGACGAGCTTCCTGATCCGGGTCTACGCCTGG
ATCGCGATCCTCAAGCCCGAGGGGCTGCTCAACCTCGCCCTGCGCCACCTCGACCTGATCGACGCGCCGCTGACCATCCT
CAACACCGACGCGGCGGTGATCGTCGGCCTCGTCTACGCCTACCTGCCCTTCATGGTGCTGCCGCTCTACGCGGTGATGA
ACCGCCTCGATCCGACCCTGTGCGAGGCCGCGGCGGATCTCGGCGCTTCGCCGAGCCGGGTGTTCTGGACCGTGACCGTG
CCGCTCAGCCTGCCGGGGATCGCGGCCGGCGCGGGGCTCTGCTTCATCCCGATGGTCGGCGAGTTCGTGATCCCCGACCT
GCTCGGCGGGTCTGAGACGCTGATGCTCGGTCGGGTGCTCTGGACCGAATTCTTCTCGAACCGCGACTGGCCGCTGGCCT
CGGCGGTCGCCGTGCTGATCCTCGCCATCGTCATCGGACCGGTGGTGCTGTTCCGGGACAGCCTGCGCGCGGGCGAGGGC
GAGGCGCCGTGA

Upstream 100 bases:

>100_bases
CCACCCTGCGGGCGAGCGGGCGGCCCGCGGAGGCCGGCGCGGCGCCGGGCTCGGACGCGCGCGCCGGTTTCGCGGCCGAC
GTCGCGGTGATCCTTCCGGC

Downstream 100 bases:

>100_bases
CCTGGGTGACCCGCACCGCCCTGGCGGCCGGCCTGGGTTTCCTCTACGGGCCGATCGCGGTGCTGATCGTGTACTCGTTC
AACGCCTCGCCGATGGTGAC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MRTGVPDEDPAAGQPGAGSARGRTRGRLFDRLVRLPPFLWLAAFFLLPFAITLKISLSEPATAIPPYLPLLDWRGGLDGW
SAFLEALNFDNYLTLAADPLYRDAALGSLTYAGVASLILVGLGTPLAYALARAPSRWQPLLVALVIVPFWTSFLIRVYAW
IAILKPEGLLNLALRHLDLIDAPLTILNTDAAVIVGLVYAYLPFMVLPLYAVMNRLDPTLCEAAADLGASPSRVFWTVTV
PLSLPGIAAGAGLCFIPMVGEFVIPDLLGGSETLMLGRVLWTEFFSNRDWPLASAVAVLILAIVIGPVVLFRDSLRAGEG
EAP

Sequences:

>Translated_323_residues
MRTGVPDEDPAAGQPGAGSARGRTRGRLFDRLVRLPPFLWLAAFFLLPFAITLKISLSEPATAIPPYLPLLDWRGGLDGW
SAFLEALNFDNYLTLAADPLYRDAALGSLTYAGVASLILVGLGTPLAYALARAPSRWQPLLVALVIVPFWTSFLIRVYAW
IAILKPEGLLNLALRHLDLIDAPLTILNTDAAVIVGLVYAYLPFMVLPLYAVMNRLDPTLCEAAADLGASPSRVFWTVTV
PLSLPGIAAGAGLCFIPMVGEFVIPDLLGGSETLMLGRVLWTEFFSNRDWPLASAVAVLILAIVIGPVVLFRDSLRAGEG
EAP
>Mature_323_residues
MRTGVPDEDPAAGQPGAGSARGRTRGRLFDRLVRLPPFLWLAAFFLLPFAITLKISLSEPATAIPPYLPLLDWRGGLDGW
SAFLEALNFDNYLTLAADPLYRDAALGSLTYAGVASLILVGLGTPLAYALARAPSRWQPLLVALVIVPFWTSFLIRVYAW
IAILKPEGLLNLALRHLDLIDAPLTILNTDAAVIVGLVYAYLPFMVLPLYAVMNRLDPTLCEAAADLGASPSRVFWTVTV
PLSLPGIAAGAGLCFIPMVGEFVIPDLLGGSETLMLGRVLWTEFFSNRDWPLASAVAVLILAIVIGPVVLFRDSLRAGEG
EAP

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=268, Percent_Identity=52.9850746268657, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1787369, Length=225, Percent_Identity=45.3333333333333, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1787713, Length=210, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34762; Mature: 34762

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTGVPDEDPAAGQPGAGSARGRTRGRLFDRLVRLPPFLWLAAFFLLPFAITLKISLSEP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCC
ATAIPPYLPLLDWRGGLDGWSAFLEALNFDNYLTLAADPLYRDAALGSLTYAGVASLILV
CCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
GLGTPLAYALARAPSRWQPLLVALVIVPFWTSFLIRVYAWIAILKPEGLLNLALRHLDLI
HCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DAPLTILNTDAAVIVGLVYAYLPFMVLPLYAVMNRLDPTLCEAAADLGASPSRVFWTVTV
HCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEE
PLSLPGIAAGAGLCFIPMVGEFVIPDLLGGSETLMLGRVLWTEFFSNRDWPLASAVAVLI
CCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LAIVIGPVVLFRDSLRAGEGEAP
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRTGVPDEDPAAGQPGAGSARGRTRGRLFDRLVRLPPFLWLAAFFLLPFAITLKISLSEP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCC
ATAIPPYLPLLDWRGGLDGWSAFLEALNFDNYLTLAADPLYRDAALGSLTYAGVASLILV
CCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
GLGTPLAYALARAPSRWQPLLVALVIVPFWTSFLIRVYAWIAILKPEGLLNLALRHLDLI
HCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DAPLTILNTDAAVIVGLVYAYLPFMVLPLYAVMNRLDPTLCEAAADLGASPSRVFWTVTV
HCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEE
PLSLPGIAAGAGLCFIPMVGEFVIPDLLGGSETLMLGRVLWTEFFSNRDWPLASAVAVLI
CCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LAIVIGPVVLFRDSLRAGEGEAP
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]