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The map label for this gene is ilvD [H]

Identifier: 170749255

GI number: 170749255

Start: 2997274

End: 2999103

Strand: Reverse

Name: ilvD [H]

Synonym: Mrad2831_2848

Alternate gene names: 170749255

Gene position: 2999103-2997274 (Counterclockwise)

Preceding gene: 170749258

Following gene: 170749251

Centisome position: 49.34

GC content: 70.16

Gene sequence:

>1830_bases
ATGCCCGCCTATCGCTCACGCACCACGACCCACGGCCGCAATATGGCCGGCGCCCGCGGCCTCTGGCGCGCCACCGGCAT
GAAGGACTCGGACTTCGGCAAGCCGATCATCGCCGTGGTGAACTCCTTCACGCAGTTCGTGCCGGGCCACGTCCACCTCA
AGGATCTCGGCCAGCTCGTCGCGCGGGAGATTGAGGCGGCCGGCGGCGTCGCCAAGGAGTTCAACACCATCGCGGTCGAT
GACGGCATCGCGATGGGCCACGACGGGATGCTGTACTCGCTGCCGTCCCGCGAATTGATCGCCGATTCCGTCGAGTACAT
GGTCAACGCGCACTGCGCCGACGCGATGGTGTGCATCTCGAACTGCGACAAGATCACCCCCGGCATGCTGATGGCGACTT
TGCGCCTCAACATCCCGACCGTGTTCGTCTCCGGCGGCCCGATGGAGGCCGGCAAGGTCCAGCTGCCGGGCATCGCCAAG
AAGGTCGACCTCGTCGACGCGATGATCGCCGCCGCCGACGACCGGATCTCCGACGAGGATGTCGCCGTCATCGAGCGCTC
GGCTTGCCCGACCTGCGGCTCCTGCTCGGGCATGTTCACGGCGAACTCGATGAACTGCCTCACCGAGGCCCTCGGGCTGG
CGCTGCCGGGCAACGGCTCGGTGCTCGCCACCCACGCCGACCGCAAGCGCCTCTTCGTCGAGGCCGGCCACCTGATCGTC
GATCTGGCCCGCCGCCACTACGAGCAGGACGACGCCGGCGTGCTGCCGCGCGCGGTCGCGAGCTTCCGGGCGTTCGAGAA
CGCCATGACCCTCGACATCGCCATGGGCGGGTCGACCAACACGGTGCTGCACCTGCTGGCCGCCGCCCACGAGGGCGAGG
TGCCCTTCACCATGGCGGACATCGACCGGCTGTCGCGGCGTGTGCCGGTGCTGTGCAAGGTCGCCCCGGCAGTGGCCAAC
GTCCACATGGAGGACGTGCATCGCGCCGGCGGGATCATGGGCATCCTGGGCGAGCTCGACCGGGCCGGCCTGATCGACAC
CAGCGTCGGCAACGTCTCTGCCGGGACGCTCGGCGCCGCGCTCGCCCGCTGGGACGTGCGCGGCGAACCGGCCGCCTCGG
TGAAGACCTTCTACCAAGCGGCGCCGGGCGGCGTGCCGACCCAGGTGGCGTTCAGTCAGGAATCCCGGTTCGACGCGCTC
GATCTCGACCGCGAGGGCGGCGTCATCCGCGACGCGGCACACGCCTATTCGCAGGATGGCGGCCTCGCCGTGCTCTACGG
CAACCTCGCCGAGCAGGGCTGTATCGTGAAGACCGCGGGCGTCGACGCCTCGATTCTGACCTTCACGGGCACCGCCCGCG
TGTTCGAGAGCCAGGACGCGGCCGTCGACGGCATCCTCAACGGCAAGGTGACGGCGGGCGAGGTCGTGCTGATCCGCTAC
GAGGGTCCCCGCGGCGGTCCCGGCATGCAGGAGATGCTCTACCCGACGAGCTACCTGAAATCGAAGGGGCTCGGGAAGGC
CTGCGCCCTCGTCACCGACGGGCGGTTCTCCGGCGGGTCGTCGGGCCTCTCCATCGGGCACGTCTCGCCGGAGGCCGCCG
AGGGCGGCCTGATCGGCCTCGTGCGCGACGGCGACCGGATCGCCATCGACATCCCGAACCGGCGAATCCAGCTCGAGGTC
GACCAGGCAGAACTGGATCAGCGGCGCAGCGCCCAGGAGGCTGCGGGCTGGCAGCCGGCGAAGCCGCGCAAGCGCAAGGT
CAGCGCGGCGCTGCGCGCCTACGCTCTGCTCACGACGAGCGCGGCGCACGGCGCGGTTCGCCGGGTCTGA

Upstream 100 bases:

>100_bases
CGCCAGAAGCATGCCACGGGTGCCAGCCCCTCCGCGACGCTTGCGGATGCGCGGGCGCGGGCGCAGAAGCCGCTCACCTT
TGAACTGGTCGAAGCTCTCG

Downstream 100 bases:

>100_bases
TCAGCGGAGTTCGAGAACCAGGGCGGTGATGGCGGCCAGCAGCGCCGCCGCTGCCACCGCGAGGCCGGTGAGGCGCGCGC
GAAGGGACCCTGTCGTCTCG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MPAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVD
DGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMATLRLNIPTVFVSGGPMEAGKVQLPGIAK
KVDLVDAMIAAADDRISDEDVAVIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKRLFVEAGHLIV
DLARRHYEQDDAGVLPRAVASFRAFENAMTLDIAMGGSTNTVLHLLAAAHEGEVPFTMADIDRLSRRVPVLCKVAPAVAN
VHMEDVHRAGGIMGILGELDRAGLIDTSVGNVSAGTLGAALARWDVRGEPAASVKTFYQAAPGGVPTQVAFSQESRFDAL
DLDREGGVIRDAAHAYSQDGGLAVLYGNLAEQGCIVKTAGVDASILTFTGTARVFESQDAAVDGILNGKVTAGEVVLIRY
EGPRGGPGMQEMLYPTSYLKSKGLGKACALVTDGRFSGGSSGLSIGHVSPEAAEGGLIGLVRDGDRIAIDIPNRRIQLEV
DQAELDQRRSAQEAAGWQPAKPRKRKVSAALRAYALLTTSAAHGAVRRV

Sequences:

>Translated_609_residues
MPAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVD
DGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMATLRLNIPTVFVSGGPMEAGKVQLPGIAK
KVDLVDAMIAAADDRISDEDVAVIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKRLFVEAGHLIV
DLARRHYEQDDAGVLPRAVASFRAFENAMTLDIAMGGSTNTVLHLLAAAHEGEVPFTMADIDRLSRRVPVLCKVAPAVAN
VHMEDVHRAGGIMGILGELDRAGLIDTSVGNVSAGTLGAALARWDVRGEPAASVKTFYQAAPGGVPTQVAFSQESRFDAL
DLDREGGVIRDAAHAYSQDGGLAVLYGNLAEQGCIVKTAGVDASILTFTGTARVFESQDAAVDGILNGKVTAGEVVLIRY
EGPRGGPGMQEMLYPTSYLKSKGLGKACALVTDGRFSGGSSGLSIGHVSPEAAEGGLIGLVRDGDRIAIDIPNRRIQLEV
DQAELDQRRSAQEAAGWQPAKPRKRKVSAALRAYALLTTSAAHGAVRRV
>Mature_608_residues
PAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDD
GIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMATLRLNIPTVFVSGGPMEAGKVQLPGIAKK
VDLVDAMIAAADDRISDEDVAVIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKRLFVEAGHLIVD
LARRHYEQDDAGVLPRAVASFRAFENAMTLDIAMGGSTNTVLHLLAAAHEGEVPFTMADIDRLSRRVPVLCKVAPAVANV
HMEDVHRAGGIMGILGELDRAGLIDTSVGNVSAGTLGAALARWDVRGEPAASVKTFYQAAPGGVPTQVAFSQESRFDALD
LDREGGVIRDAAHAYSQDGGLAVLYGNLAEQGCIVKTAGVDASILTFTGTARVFESQDAAVDGILNGKVTAGEVVLIRYE
GPRGGPGMQEMLYPTSYLKSKGLGKACALVTDGRFSGGSSGLSIGHVSPEAAEGGLIGLVRDGDRIAIDIPNRRIQLEVD
QAELDQRRSAQEAAGWQPAKPRKRKVSAALRAYALLTTSAAHGAVRRV

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=610, Percent_Identity=74.4262295081967, Blast_Score=922, Evalue=0.0,
Organism=Escherichia coli, GI2367371, Length=589, Percent_Identity=29.2020373514431, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1788157, Length=548, Percent_Identity=29.3795620437956, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1786464, Length=521, Percent_Identity=31.2859884836852, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322476, Length=614, Percent_Identity=37.785016286645, Blast_Score=357, Evalue=4e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 64084; Mature: 63953

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHCCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHH
NCDKITPGMLMATLRLNIPTVFVSGGPMEAGKVQLPGIAKKVDLVDAMIAAADDRISDED
CCCCCCCCCEEEEEEECCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCC
VAVIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKRLFVEAGHLIV
CEEEECCCCCCCCCCCCCEECCHHHHHHHHHCEEECCCCCEEEEECCCCEEEHHHHHHHH
DLARRHYEQDDAGVLPRAVASFRAFENAMTLDIAMGGSTNTVLHLLAAAHEGEVPFTMAD
HHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEHHH
IDRLSRRVPVLCKVAPAVANVHMEDVHRAGGIMGILGELDRAGLIDTSVGNVSAGTLGAA
HHHHHCCCCEEEEECHHHHHCCHHHHHHHCCHHHHHHCCCCCCCEECCCCCCCCCHHHHH
LARWDVRGEPAASVKTFYQAAPGGVPTQVAFSQESRFDALDLDREGGVIRDAAHAYSQDG
HHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCEEHHHHHHHCCCC
GLAVLYGNLAEQGCIVKTAGVDASILTFTGTARVFESQDAAVDGILNGKVTAGEVVLIRY
CEEEEECCCCCCCCEEEECCCCEEEEEEECCHHEECCCCCCCCCEECCCEEECEEEEEEE
EGPRGGPGMQEMLYPTSYLKSKGLGKACALVTDGRFSGGSSGLSIGHVSPEAAEGGLIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEE
VRDGDRIAIDIPNRRIQLEVDQAELDQRRSAQEAAGWQPAKPRKRKVSAALRAYALLTTS
EECCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
AAHGAVRRV
HHCCHHCCC
>Mature Secondary Structure 
PAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHCCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHH
NCDKITPGMLMATLRLNIPTVFVSGGPMEAGKVQLPGIAKKVDLVDAMIAAADDRISDED
CCCCCCCCCEEEEEEECCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCC
VAVIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKRLFVEAGHLIV
CEEEECCCCCCCCCCCCCEECCHHHHHHHHHCEEECCCCCEEEEECCCCEEEHHHHHHHH
DLARRHYEQDDAGVLPRAVASFRAFENAMTLDIAMGGSTNTVLHLLAAAHEGEVPFTMAD
HHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEHHH
IDRLSRRVPVLCKVAPAVANVHMEDVHRAGGIMGILGELDRAGLIDTSVGNVSAGTLGAA
HHHHHCCCCEEEEECHHHHHCCHHHHHHHCCHHHHHHCCCCCCCEECCCCCCCCCHHHHH
LARWDVRGEPAASVKTFYQAAPGGVPTQVAFSQESRFDALDLDREGGVIRDAAHAYSQDG
HHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCEEHHHHHHHCCCC
GLAVLYGNLAEQGCIVKTAGVDASILTFTGTARVFESQDAAVDGILNGKVTAGEVVLIRY
CEEEEECCCCCCCCEEEECCCCEEEEEEECCHHEECCCCCCCCCEECCCEEECEEEEEEE
EGPRGGPGMQEMLYPTSYLKSKGLGKACALVTDGRFSGGSSGLSIGHVSPEAAEGGLIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEE
VRDGDRIAIDIPNRRIQLEVDQAELDQRRSAQEAAGWQPAKPRKRKVSAALRAYALLTTS
EECCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
AAHGAVRRV
HHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA