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The map label for this gene is ppdK [H]

Identifier: 170749215

GI number: 170749215

Start: 2951413

End: 2954103

Strand: Reverse

Name: ppdK [H]

Synonym: Mrad2831_2808

Alternate gene names: 170749215

Gene position: 2954103-2951413 (Counterclockwise)

Preceding gene: 170749216

Following gene: 170749214

Centisome position: 48.6

GC content: 68.64

Gene sequence:

>2691_bases
ATGGCGACCGGCAGCGCGAAGTGGGTCTATGCCTTCGGCGACGGCAAGGCGGAGGGCAAGTCGCAGATGCGCGACTTGCT
CGGCGGCAAGGGCGCCAATCTGGCCGAGATGTCCAACCTGGGCCTGCCCGTCCCGCCCGGCTTCACCATCACCACCGAGG
TCTGCACCTACTTCTACGATCACGGCAAGGCCTACCCGCCGGAGCTGAAGGACCAGGTCTCGGCCGCGCTCGCGCAGGTC
GGCGCGCTCACCGGCCGCAAGTTCGGCGACTCGACCAATCCGCTGCTCGTCTCGGTCCGCTCGGGCGCCCGGGCCTCGAT
GCCCGGCATGATGGACACGGTGCTCAATCTCGGCCTCAACGACGAGACCGTCCTGGCCCTCGCCAAGGAGGCCGACGACG
AGCGCTTCGCCTACGATTCCTATCGCCGCTTCATCACCATGTACTCCAACGTCGTCCTCGGCGTGGAGCACCACGCCTTC
GAGGAGGCGCTGGAGCAGTACAAGGAAGGCAAGGGCTTCGACCTCGACACCGAGCTCGGCGCCGGCGACTGGAAGACCCT
CATCCAGACCTACAAGAAGATCGTGCGCGACGAGCACGGCTCCGACTTCCCGCAGGGGGCGGACGACCAGCTCTGGGGCG
CGATCGGCGCCGTGTTCAATTCCTGGATGATTCCCCGCGCGAAGAAGTACCGGGAGCTGAACAACATCCCGGAGAGCTGG
GGCACGGCCGTCAACGTGCAGGCCATGGTGTTCGGCAACATGGGCGACACCTCGGCCACCGGCGTCGCCTTCACCCGCAA
TCCCTCCACCGGCGAGAAGGCGCTGTACGGCGAGTTCCTGATCAACGCGCAGGGCGAGGACGTGGTGGCGGGCATCCGCA
CGCCGCAGAACATCACCGAGAAGGCGCGGGAAGAGGCCGGAAGCGACAAGCCTTCGATGGAGAAGGCCATGCCCGAGAGC
TTCGCGGAGCTGACCCGGATCTACGGGATCCTGGAGTCGCATTACCGTGACATGCAGGACATGGAGTTCACCATCGAGAA
GGGGAAGCTCTGGATGCTCCAGACCCGCAACGGCAAGCGCACGGCCAAGGCCGCCCTGCGCATCGCCGTCGATCTGGCGG
GCGAGGGGCTGATCTCCCGCGAGGAGGCGATCGGCCGCGTCGAGCCCGGCGCCCTCGATCAGCTCCTGCACCCGACGATC
GACCCGAAGGCCGAGCGCAAGGTCATCGCCTCCGGCCTGCCGGCCTCGCCCGGCGCGGCGACCGGCGAGATCGTGTTCAA
CTCCGAGGACGCGGAGGCGCACCGCAAGGCCGAGCGCAAGTGCATCCTGGTGCGGATCGAGACCTCGCCCGAGGACATCC
ACGGCATGCACGCCGCCGAGGGCATCCTCACCACCCGCGGCGGCATGACCAGCCACGCGGCCGTCGTCGCGCGCGGCATG
GGCAAGCCCTGCGTCTCCGGCGTCGGCTCGATCCGGATCGACTACAAGGCGCAGACCCTCACGGTCGGAGGCACGACGCT
GAAGGCCGGCGACAAGATCACCATCGACGGCTCCACCGGTCAGGTGATCCAGGGTGAGGTGAAGATGCTGGAGCCCGAGC
TCTCCGGCGATTTCGCCACGCTGATGGAGTGGGCGGACGCGTTCCGGGCCATGAAGGTCCGGGCGAACGCCGACACCCCG
ACCGATGCGCGCACCGCCCGCAACTTCGGCGCCGAGGGCATCGGCCTCTGCCGCACGGAGCACATGTTCTTCGAGGGCGA
CCGCATCATCGCGGTGCGCGAGATGATCCTGGCGGACGACGCCGAGGGCCGCCGCGCGGCGCTGGCGAAGATCCTGCCCT
ACCAGCGCCAGGACTTCCTGGAGCTGTTCACGATCATGTCCGGCCTGCCGGTCACGATCCGCCTGCTCGACCCGCCGCTC
CACGAGTTCCTGCCGCACACCGACGCCGAGGTCGCCGAAGTCGTGAAGGCGACGGGCGTCTCCGAGGAGAAGATCCGGCA
TCGGATGCGCGAGCTCTCCGAGCATAACCCGATGCTCGGCTTCCGCGGCTGCCGCCTGGCGATCGCCTTCCCGGAGATCG
CCGAGATGCAGGCCCGGGCCATCTTCGAGGCCGCCGTCCAGGCTGCCGCGGACACCGGCAAGGCAGTGACGCCCGAAGTC
ATGGTGCCGCTGGTCTTCACCCGCGCCGAGTTCGACATCGTGAAGGCCCGCATCGACGCCATGGCGAGGGCCGTGTCCGA
GGAAAAGGGCGCGACCCTCGACTACCAAGTCGGCACGATGATCGAGCTGCCGCGCGCCGCCCTCAAGGCCGGCGAGATCG
CCGAGACCGCGGAGTTCTTCTCCTTCGGCACCAACGACCTGACGCAGACGGCGCTCGGCATCTCCCGCGACGACGCCGCC
ACCTTCCTGGGGCCCTACACCCAGAAGGGCATCCTGCCGGCCGATCCGTTCATCACGATCGATCAGGAGGGCGTGGGCGA
GCTGGTGCGCATCGGCGTGGAGCGCGGCCGCAAGACGCGTCCGGACATCAAGCTCGGCATCTGCGGCGAGCACGGCGGCG
ACCCCGCCTCGGTGGGCTTCTGCCAGGAGGTCGGGCTCGATTACGTGTCCTGCTCGCCCTACCGGGTGCCGATCGCCCGG
CTCGCGGCCGCCCAGGCAGCGCTGGGCAAGGTCGCCGGCAAGGAAGGCTGA

Upstream 100 bases:

>100_bases
ACAGGGCCATCGCGCGTTCATCTTGCGTTGCGTTGCGGGATGGTCCAACCCCGCCCATCAGCTCGGCCGGCGCCGGGCAC
TAGATACGGACGGGATCGAC

Downstream 100 bases:

>100_bases
GGCCGGTCCGGGGGGCGCCGAGCGCGTCCCCCTTTCGGCTATCAACCGTCCGGACGAGGATCAGTGGAAAACTCGCCTTT
CGGTCGAACTCGCCTCCTCG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 896; Mature: 895

Protein sequence:

>896_residues
MATGSAKWVYAFGDGKAEGKSQMRDLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYFYDHGKAYPPELKDQVSAALAQV
GALTGRKFGDSTNPLLVSVRSGARASMPGMMDTVLNLGLNDETVLALAKEADDERFAYDSYRRFITMYSNVVLGVEHHAF
EEALEQYKEGKGFDLDTELGAGDWKTLIQTYKKIVRDEHGSDFPQGADDQLWGAIGAVFNSWMIPRAKKYRELNNIPESW
GTAVNVQAMVFGNMGDTSATGVAFTRNPSTGEKALYGEFLINAQGEDVVAGIRTPQNITEKAREEAGSDKPSMEKAMPES
FAELTRIYGILESHYRDMQDMEFTIEKGKLWMLQTRNGKRTAKAALRIAVDLAGEGLISREEAIGRVEPGALDQLLHPTI
DPKAERKVIASGLPASPGAATGEIVFNSEDAEAHRKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGM
GKPCVSGVGSIRIDYKAQTLTVGGTTLKAGDKITIDGSTGQVIQGEVKMLEPELSGDFATLMEWADAFRAMKVRANADTP
TDARTARNFGAEGIGLCRTEHMFFEGDRIIAVREMILADDAEGRRAALAKILPYQRQDFLELFTIMSGLPVTIRLLDPPL
HEFLPHTDAEVAEVVKATGVSEEKIRHRMRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAADTGKAVTPEV
MVPLVFTRAEFDIVKARIDAMARAVSEEKGATLDYQVGTMIELPRAALKAGEIAETAEFFSFGTNDLTQTALGISRDDAA
TFLGPYTQKGILPADPFITIDQEGVGELVRIGVERGRKTRPDIKLGICGEHGGDPASVGFCQEVGLDYVSCSPYRVPIAR
LAAAQAALGKVAGKEG

Sequences:

>Translated_896_residues
MATGSAKWVYAFGDGKAEGKSQMRDLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYFYDHGKAYPPELKDQVSAALAQV
GALTGRKFGDSTNPLLVSVRSGARASMPGMMDTVLNLGLNDETVLALAKEADDERFAYDSYRRFITMYSNVVLGVEHHAF
EEALEQYKEGKGFDLDTELGAGDWKTLIQTYKKIVRDEHGSDFPQGADDQLWGAIGAVFNSWMIPRAKKYRELNNIPESW
GTAVNVQAMVFGNMGDTSATGVAFTRNPSTGEKALYGEFLINAQGEDVVAGIRTPQNITEKAREEAGSDKPSMEKAMPES
FAELTRIYGILESHYRDMQDMEFTIEKGKLWMLQTRNGKRTAKAALRIAVDLAGEGLISREEAIGRVEPGALDQLLHPTI
DPKAERKVIASGLPASPGAATGEIVFNSEDAEAHRKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGM
GKPCVSGVGSIRIDYKAQTLTVGGTTLKAGDKITIDGSTGQVIQGEVKMLEPELSGDFATLMEWADAFRAMKVRANADTP
TDARTARNFGAEGIGLCRTEHMFFEGDRIIAVREMILADDAEGRRAALAKILPYQRQDFLELFTIMSGLPVTIRLLDPPL
HEFLPHTDAEVAEVVKATGVSEEKIRHRMRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAADTGKAVTPEV
MVPLVFTRAEFDIVKARIDAMARAVSEEKGATLDYQVGTMIELPRAALKAGEIAETAEFFSFGTNDLTQTALGISRDDAA
TFLGPYTQKGILPADPFITIDQEGVGELVRIGVERGRKTRPDIKLGICGEHGGDPASVGFCQEVGLDYVSCSPYRVPIAR
LAAAQAALGKVAGKEG
>Mature_895_residues
ATGSAKWVYAFGDGKAEGKSQMRDLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYFYDHGKAYPPELKDQVSAALAQVG
ALTGRKFGDSTNPLLVSVRSGARASMPGMMDTVLNLGLNDETVLALAKEADDERFAYDSYRRFITMYSNVVLGVEHHAFE
EALEQYKEGKGFDLDTELGAGDWKTLIQTYKKIVRDEHGSDFPQGADDQLWGAIGAVFNSWMIPRAKKYRELNNIPESWG
TAVNVQAMVFGNMGDTSATGVAFTRNPSTGEKALYGEFLINAQGEDVVAGIRTPQNITEKAREEAGSDKPSMEKAMPESF
AELTRIYGILESHYRDMQDMEFTIEKGKLWMLQTRNGKRTAKAALRIAVDLAGEGLISREEAIGRVEPGALDQLLHPTID
PKAERKVIASGLPASPGAATGEIVFNSEDAEAHRKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMG
KPCVSGVGSIRIDYKAQTLTVGGTTLKAGDKITIDGSTGQVIQGEVKMLEPELSGDFATLMEWADAFRAMKVRANADTPT
DARTARNFGAEGIGLCRTEHMFFEGDRIIAVREMILADDAEGRRAALAKILPYQRQDFLELFTIMSGLPVTIRLLDPPLH
EFLPHTDAEVAEVVKATGVSEEKIRHRMRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAADTGKAVTPEVM
VPLVFTRAEFDIVKARIDAMARAVSEEKGATLDYQVGTMIELPRAALKAGEIAETAEFFSFGTNDLTQTALGISRDDAAT
FLGPYTQKGILPADPFITIDQEGVGELVRIGVERGRKTRPDIKLGICGEHGGDPASVGFCQEVGLDYVSCSPYRVPIARL
AAAQAALGKVAGKEG

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=459, Percent_Identity=24.6187363834423, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1787994, Length=716, Percent_Identity=24.3016759776536, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1789193, Length=416, Percent_Identity=25.9615384615385, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788726, Length=470, Percent_Identity=23.6170212765957, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96954; Mature: 96823

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGSAKWVYAFGDGKAEGKSQMRDLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYFYD
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEHHHHHHHHHH
HGKAYPPELKDQVSAALAQVGALTGRKFGDSTNPLLVSVRSGARASMPGMMDTVLNLGLN
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCC
DETVLALAKEADDERFAYDSYRRFITMYSNVVLGVEHHAFEEALEQYKEGKGFDLDTELG
CHHEEEEEHHCCCCHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCCCCCCCCCC
AGDWKTLIQTYKKIVRDEHGSDFPQGADDQLWGAIGAVFNSWMIPRAKKYRELNNIPESW
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
GTAVNVQAMVFGNMGDTSATGVAFTRNPSTGEKALYGEFLINAQGEDVVAGIRTPQNITE
CCEEEEEEEEEECCCCCCCCCEEEECCCCCCCHHEEEEEEECCCCCCEEEECCCCHHHHH
KAREEAGSDKPSMEKAMPESFAELTRIYGILESHYRDMQDMEFTIEKGKLWMLQTRNGKR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEECCCEEEEEECCCCH
TAKAALRIAVDLAGEGLISREEAIGRVEPGALDQLLHPTIDPKAERKVIASGLPASPGAA
HHHHHHHEEEEECCCCCCCHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
TGEIVFNSEDAEAHRKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGM
CCEEEECCCCHHHHHHHCCCEEEEEEECCCHHHCCHHHHCCEEEECCCCCHHHHHHHHCC
GKPCVSGVGSIRIDYKAQTLTVGGTTLKAGDKITIDGSTGQVIQGEVKMLEPELSGDFAT
CCHHHCCCCCEEEEEEEEEEEECCEEEECCCEEEEECCCCCEEECCHHEECCCCCCCHHH
LMEWADAFRAMKVRANADTPTDARTARNFGAEGIGLCRTEHMFFEGDRIIAVREMILADD
HHHHHHHHHHEEEECCCCCCCHHHHHHHCCCCCCEEEEECCEEECCCEEEEHHHHHHHCC
AEGRRAALAKILPYQRQDFLELFTIMSGLPVTIRLLDPPLHEFLPHTDAEVAEVVKATGV
CCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHCCC
SEEKIRHRMRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAADTGKAVTPEV
CHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MVPLVFTRAEFDIVKARIDAMARAVSEEKGATLDYQVGTMIELPRAALKAGEIAETAEFF
HHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCHHHHHHCCHHHHHHHH
SFGTNDLTQTALGISRDDAATFLGPYTQKGILPADPFITIDQEGVGELVRIGVERGRKTR
HCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCC
PDIKLGICGEHGGDPASVGFCQEVGLDYVSCSPYRVPIARLAAAQAALGKVAGKEG
CCEEEEECCCCCCCCCCCHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATGSAKWVYAFGDGKAEGKSQMRDLLGGKGANLAEMSNLGLPVPPGFTITTEVCTYFYD
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEHHHHHHHHHH
HGKAYPPELKDQVSAALAQVGALTGRKFGDSTNPLLVSVRSGARASMPGMMDTVLNLGLN
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCC
DETVLALAKEADDERFAYDSYRRFITMYSNVVLGVEHHAFEEALEQYKEGKGFDLDTELG
CHHEEEEEHHCCCCHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCCCCCCCCCC
AGDWKTLIQTYKKIVRDEHGSDFPQGADDQLWGAIGAVFNSWMIPRAKKYRELNNIPESW
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
GTAVNVQAMVFGNMGDTSATGVAFTRNPSTGEKALYGEFLINAQGEDVVAGIRTPQNITE
CCEEEEEEEEEECCCCCCCCCEEEECCCCCCCHHEEEEEEECCCCCCEEEECCCCHHHHH
KAREEAGSDKPSMEKAMPESFAELTRIYGILESHYRDMQDMEFTIEKGKLWMLQTRNGKR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEECCCEEEEEECCCCH
TAKAALRIAVDLAGEGLISREEAIGRVEPGALDQLLHPTIDPKAERKVIASGLPASPGAA
HHHHHHHEEEEECCCCCCCHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
TGEIVFNSEDAEAHRKAERKCILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGM
CCEEEECCCCHHHHHHHCCCEEEEEEECCCHHHCCHHHHCCEEEECCCCCHHHHHHHHCC
GKPCVSGVGSIRIDYKAQTLTVGGTTLKAGDKITIDGSTGQVIQGEVKMLEPELSGDFAT
CCHHHCCCCCEEEEEEEEEEEECCEEEECCCEEEEECCCCCEEECCHHEECCCCCCCHHH
LMEWADAFRAMKVRANADTPTDARTARNFGAEGIGLCRTEHMFFEGDRIIAVREMILADD
HHHHHHHHHHEEEECCCCCCCHHHHHHHCCCCCCEEEEECCEEECCCEEEEHHHHHHHCC
AEGRRAALAKILPYQRQDFLELFTIMSGLPVTIRLLDPPLHEFLPHTDAEVAEVVKATGV
CCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHCCC
SEEKIRHRMRELSEHNPMLGFRGCRLAIAFPEIAEMQARAIFEAAVQAAADTGKAVTPEV
CHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MVPLVFTRAEFDIVKARIDAMARAVSEEKGATLDYQVGTMIELPRAALKAGEIAETAEFF
HHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCHHHHHHCCHHHHHHHH
SFGTNDLTQTALGISRDDAATFLGPYTQKGILPADPFITIDQEGVGELVRIGVERGRKTR
HCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCC
PDIKLGICGEHGGDPASVGFCQEVGLDYVSCSPYRVPIARLAAAQAALGKVAGKEG
CCEEEEECCCCCCCCCCCHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]