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The map label for this gene is rpoN2 [H]

Identifier: 170748838

GI number: 170748838

Start: 2583554

End: 2585128

Strand: Reverse

Name: rpoN2 [H]

Synonym: Mrad2831_2420

Alternate gene names: 170748838

Gene position: 2585128-2583554 (Counterclockwise)

Preceding gene: 170748841

Following gene: 170748837

Centisome position: 42.53

GC content: 72.44

Gene sequence:

>1575_bases
ATGAGTTTGGTGCAACGGCTGGAAGTGCGTCAGGGCCAGGCCCTGGTGATGACGCCGCAGCTCCTGCAGGCGATCAAGCT
TCTGCAATTGTCGCACCTCGATCTCTCGGCCTACGTCGAGGCCGAGCTGGAGCGCAACCCGCTGCTGGAGCGCGCCGAGC
CGGACCTGCCGGCCGCGGGCGATGCCGCCGAATCGGGCGGCCACGCCGACGAGGCGGGGTCGGACGGGTTCGAGGCGGCT
GAGCCCGAATCGTGGCTCTCGGCCGACCTCAACCCGAGCCGCGGCGAGATCGAGAGCGACTTCGACACGCGCCTCGACAA
CGTCTTCCCCGACGAGAGCCCCGTCCAGGCGCGCGAGGCGGGCGGGGGCGACATGCTCTCGCTGACGCCGCCGCCCTACG
GCAGCACCGGCGGCAGCTTCGACGCCGAGGCCCCCGACTTCGAGGCGACCCTGACGGCCGAGACCTCGCTGCGCGAGCAC
CTCGCGGGCCAGCTCGACCTCGCGACCCGGGATCCGGCCGAGCGGCTGATCGGCGGGTTCCTGATCGACGCCGTCGACGA
GGCCGGCTACCTGCGCGAGACGGTGGAGGGCGTGGCCGAGCGCCTCGGCGCCGACCCGGCCCTCGTCGGCCGGATGCTGG
CGCTGGTCCAGACCTTCGACCCGCCCGGGATCGCGGCCCGCGACCTCGCCGAGTGCCTCGCGATCCAGCTGCGCGAGCGC
GACCGGTTCGACCCGGCGATGCAGGCGCTGGTCTCGCGGCTGGACCTCGTGGCCAAGCGGGACTTCGCCGCGCTGCGCCG
CCTCTGCGGCGTCGACGACGAGGACCTCGTCGACATGCTGGGCGAGATCCGGCGCCTGGACCCGAAGCCGGGCCGGGCCT
TCGGGTCGAGCGCCGTCGAGGTCCTGGTTCCGGACGTGTTCGTGCGCGCCGCGCCGGACGGCTCCTGGCTGGTCGAGCTG
AACAGCGAGGCGCTGCCCCGGGTCCTGGTGAACCAGAGCTACTACGCCCGCGTCTCGCGCAACGCCGCCGCGGAGGGCGA
CAAGGCGTTCCTGTCGGAGGCGCTGCAGAACGCCAACTGGCTGACCCGCTCGCTGGAGCAGCGTGCCCGCACCATCCTGA
AGGTCGCCACCGAGATCGTCCGCCAGCAGGACGGTTTCTTCGTCCACGGCGTCACCCACCTGCGGCCGCTGAACCTCAAG
ACCGTGGCCGAGGCGATCGGCATGCACGAATCGACCGTCTCGCGGGTCACCTCCAACAAGGCCATCGGCACGAGCCGGGG
GACCCTGGAGATGAAGTACTTCTTCACCGCGGCGATCCCCGGGGCGGCCGGCGCCGCCGCCCACTCCTCGGAGGCGGTGC
GCCACCGCATCAAGCAGCTCGTCGACGGCGAGACGCCGGACGACGTGCTGTCCGACGACGCGCTGGTGCAGAAGCTGCGC
GGGGAGGGCGTCGACATCGCCCGGCGCACGGTGGCGAAGTACCGCGAGTCCCTGCGGATCCCGAGCTCGATCGAGCGCCG
CCGGGAGCGTTTCGCCCACGGCTCCAGCCAGCACGTCGCCCTGGCGCTGCGCTGA

Upstream 100 bases:

>100_bases
GGATCGGGCTAGAGAAAGCGCAACGGAACAAGCAAGAACCATGCCGTGAGCAGCGTGGCTCCGGCTGGCGAACCGCGCCG
GAAGGGCATCGGGGCCTCGC

Downstream 100 bases:

>100_bases
TCGCTCCGGACGGGGTTGCGCCGGGCGCCCGTCCGGCCCATCCGTCGTGGCCTTCGATCGACGCCTGGACCTGCGATGAC
CCCGACCGGAACGCCCTCGC

Product: RNA polymerase, sigma 54 subunit, RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSLVQRLEVRQGQALVMTPQLLQAIKLLQLSHLDLSAYVEAELERNPLLERAEPDLPAAGDAAESGGHADEAGSDGFEAA
EPESWLSADLNPSRGEIESDFDTRLDNVFPDESPVQAREAGGGDMLSLTPPPYGSTGGSFDAEAPDFEATLTAETSLREH
LAGQLDLATRDPAERLIGGFLIDAVDEAGYLRETVEGVAERLGADPALVGRMLALVQTFDPPGIAARDLAECLAIQLRER
DRFDPAMQALVSRLDLVAKRDFAALRRLCGVDDEDLVDMLGEIRRLDPKPGRAFGSSAVEVLVPDVFVRAAPDGSWLVEL
NSEALPRVLVNQSYYARVSRNAAAEGDKAFLSEALQNANWLTRSLEQRARTILKVATEIVRQQDGFFVHGVTHLRPLNLK
TVAEAIGMHESTVSRVTSNKAIGTSRGTLEMKYFFTAAIPGAAGAAAHSSEAVRHRIKQLVDGETPDDVLSDDALVQKLR
GEGVDIARRTVAKYRESLRIPSSIERRRERFAHGSSQHVALALR

Sequences:

>Translated_524_residues
MSLVQRLEVRQGQALVMTPQLLQAIKLLQLSHLDLSAYVEAELERNPLLERAEPDLPAAGDAAESGGHADEAGSDGFEAA
EPESWLSADLNPSRGEIESDFDTRLDNVFPDESPVQAREAGGGDMLSLTPPPYGSTGGSFDAEAPDFEATLTAETSLREH
LAGQLDLATRDPAERLIGGFLIDAVDEAGYLRETVEGVAERLGADPALVGRMLALVQTFDPPGIAARDLAECLAIQLRER
DRFDPAMQALVSRLDLVAKRDFAALRRLCGVDDEDLVDMLGEIRRLDPKPGRAFGSSAVEVLVPDVFVRAAPDGSWLVEL
NSEALPRVLVNQSYYARVSRNAAAEGDKAFLSEALQNANWLTRSLEQRARTILKVATEIVRQQDGFFVHGVTHLRPLNLK
TVAEAIGMHESTVSRVTSNKAIGTSRGTLEMKYFFTAAIPGAAGAAAHSSEAVRHRIKQLVDGETPDDVLSDDALVQKLR
GEGVDIARRTVAKYRESLRIPSSIERRRERFAHGSSQHVALALR
>Mature_523_residues
SLVQRLEVRQGQALVMTPQLLQAIKLLQLSHLDLSAYVEAELERNPLLERAEPDLPAAGDAAESGGHADEAGSDGFEAAE
PESWLSADLNPSRGEIESDFDTRLDNVFPDESPVQAREAGGGDMLSLTPPPYGSTGGSFDAEAPDFEATLTAETSLREHL
AGQLDLATRDPAERLIGGFLIDAVDEAGYLRETVEGVAERLGADPALVGRMLALVQTFDPPGIAARDLAECLAIQLRERD
RFDPAMQALVSRLDLVAKRDFAALRRLCGVDDEDLVDMLGEIRRLDPKPGRAFGSSAVEVLVPDVFVRAAPDGSWLVELN
SEALPRVLVNQSYYARVSRNAAAEGDKAFLSEALQNANWLTRSLEQRARTILKVATEIVRQQDGFFVHGVTHLRPLNLKT
VAEAIGMHESTVSRVTSNKAIGTSRGTLEMKYFFTAAIPGAAGAAAHSSEAVRHRIKQLVDGETPDDVLSDDALVQKLRG
EGVDIARRTVAKYRESLRIPSSIERRRERFAHGSSQHVALALR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=513, Percent_Identity=37.4269005847953, Blast_Score=321, Evalue=8e-89,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56921; Mature: 56789

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVQRLEVRQGQALVMTPQLLQAIKLLQLSHLDLSAYVEAELERNPLLERAEPDLPAAG
CCHHHHHHHHCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCC
DAAESGGHADEAGSDGFEAAEPESWLSADLNPSRGEIESDFDTRLDNVFPDESPVQAREA
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHC
GGGDMLSLTPPPYGSTGGSFDAEAPDFEATLTAETSLREHLAGQLDLATRDPAERLIGGF
CCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LIDAVDEAGYLRETVEGVAERLGADPALVGRMLALVQTFDPPGIAARDLAECLAIQLRER
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DRFDPAMQALVSRLDLVAKRDFAALRRLCGVDDEDLVDMLGEIRRLDPKPGRAFGSSAVE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCHHH
VLVPDVFVRAAPDGSWLVELNSEALPRVLVNQSYYARVSRNAAAEGDKAFLSEALQNANW
HHHCHHHEEECCCCCEEEEECCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LTRSLEQRARTILKVATEIVRQQDGFFVHGVTHLRPLNLKTVAEAIGMHESTVSRVTSNK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCHHHHHHHHCCCHHHHHHHHCCC
AIGTSRGTLEMKYFFTAAIPGAAGAAAHSSEAVRHRIKQLVDGETPDDVLSDDALVQKLR
CCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHC
GEGVDIARRTVAKYRESLRIPSSIERRRERFAHGSSQHVALALR
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
SLVQRLEVRQGQALVMTPQLLQAIKLLQLSHLDLSAYVEAELERNPLLERAEPDLPAAG
CHHHHHHHHCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCC
DAAESGGHADEAGSDGFEAAEPESWLSADLNPSRGEIESDFDTRLDNVFPDESPVQAREA
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHC
GGGDMLSLTPPPYGSTGGSFDAEAPDFEATLTAETSLREHLAGQLDLATRDPAERLIGGF
CCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LIDAVDEAGYLRETVEGVAERLGADPALVGRMLALVQTFDPPGIAARDLAECLAIQLRER
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DRFDPAMQALVSRLDLVAKRDFAALRRLCGVDDEDLVDMLGEIRRLDPKPGRAFGSSAVE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCHHH
VLVPDVFVRAAPDGSWLVELNSEALPRVLVNQSYYARVSRNAAAEGDKAFLSEALQNANW
HHHCHHHEEECCCCCEEEEECCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LTRSLEQRARTILKVATEIVRQQDGFFVHGVTHLRPLNLKTVAEAIGMHESTVSRVTSNK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCHHHHHHHHCCCHHHHHHHHCCC
AIGTSRGTLEMKYFFTAAIPGAAGAAAHSSEAVRHRIKQLVDGETPDDVLSDDALVQKLR
CCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHC
GEGVDIARRTVAKYRESLRIPSSIERRRERFAHGSSQHVALALR
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]