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The map label for this gene is phoB [H]

Identifier: 170748757

GI number: 170748757

Start: 2474331

End: 2475026

Strand: Reverse

Name: phoB [H]

Synonym: Mrad2831_2339

Alternate gene names: 170748757

Gene position: 2475026-2474331 (Counterclockwise)

Preceding gene: 170748758

Following gene: 170748754

Centisome position: 40.72

GC content: 72.41

Gene sequence:

>696_bases
ATGCAAGTCCTGATCGTCGAGGACGAGGAGGCGCTGACCACGCTGCTGCGCTACAACCTGGAGGCCGAGGGCTTCCTGGT
GGATTCGGCGGCGCGCGGCGACGACGCCGAGCTGCTCCTGGCGGAGCGGATCCCGGACCTCGTCCTGCTCGACTGGATGC
TGCCCGGCCTCTCCGGGATCGAGCTGTGCCGCCGGATCCGGGCGCGCCGGGAGACCGAGCGCCTGCCGGTGATCATGCTC
ACCGCCCGGGGCGAGGAGGGCGACCGCGTCCGCGGTCTCGGCACGGGCGCCGACGACTACATCGTCAAGCCGTTCTCGGT
GCCGGAACTGCTCGCCCGGATCCGCGCGCTGCTGCGCCGGGCCAAGCCCGCCCACGTCTCGGACCGGCTGGCGGCGGGCG
ATTTGGAGCTCGACCGCACCGGCCACCGCGTCCGTCGCGGCGGCGAGGAGCTGCATCTGGGCCCGACAGAGTTCCGGCTG
CTGGAATTCCTGATGCTGGCGCCGGGCCGGGTCTTCTCCCGCGAGCAGCTCCTCGACGGGGTCTGGGGCCACGACGTCTA
CATCGACGAGCGCACGGTGGACGTGCATGTCGGGCGGCTGCGCAAGGCGCTGAACGGCCCGCGCCAGGCCGACCCGATCC
GCACGGTCCGCGGCTCGGGCTACGCCTTCGACGAGACCTTCTCGCTCGAGGCCTGA

Upstream 100 bases:

>100_bases
GCGGGCATGGCCGGGGCCAGTAACCGTGCAGGTTGAGGCCGGGCGGCGCCTCCTGCACCATCCGCGGGACCGCCGGTAGA
ACGAGTCGTCGAACGTGAGC

Downstream 100 bases:

>100_bases
GGAGGCCCCATGGACTGCCGGAGCGACAGATCGCTCCGGCGCGCCGTCTCTGCTCAGGCGCCCGAGACCGCGGACGGGTT
CAGCGCGCCAGCGCCGCGCT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MQVLIVEDEEALTTLLRYNLEAEGFLVDSAARGDDAELLLAERIPDLVLLDWMLPGLSGIELCRRIRARRETERLPVIML
TARGEEGDRVRGLGTGADDYIVKPFSVPELLARIRALLRRAKPAHVSDRLAAGDLELDRTGHRVRRGGEELHLGPTEFRL
LEFLMLAPGRVFSREQLLDGVWGHDVYIDERTVDVHVGRLRKALNGPRQADPIRTVRGSGYAFDETFSLEA

Sequences:

>Translated_231_residues
MQVLIVEDEEALTTLLRYNLEAEGFLVDSAARGDDAELLLAERIPDLVLLDWMLPGLSGIELCRRIRARRETERLPVIML
TARGEEGDRVRGLGTGADDYIVKPFSVPELLARIRALLRRAKPAHVSDRLAAGDLELDRTGHRVRRGGEELHLGPTEFRL
LEFLMLAPGRVFSREQLLDGVWGHDVYIDERTVDVHVGRLRKALNGPRQADPIRTVRGSGYAFDETFSLEA
>Mature_231_residues
MQVLIVEDEEALTTLLRYNLEAEGFLVDSAARGDDAELLLAERIPDLVLLDWMLPGLSGIELCRRIRARRETERLPVIML
TARGEEGDRVRGLGTGADDYIVKPFSVPELLARIRALLRRAKPAHVSDRLAAGDLELDRTGHRVRRGGEELHLGPTEFRL
LEFLMLAPGRVFSREQLLDGVWGHDVYIDERTVDVHVGRLRKALNGPRQADPIRTVRGSGYAFDETFSLEA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=52.6548672566372, Blast_Score=228, Evalue=2e-61,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=43.1111111111111, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=40.8888888888889, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=38.3928571428571, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=40.7894736842105, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=37.219730941704, Blast_Score=143, Evalue=8e-36,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=38.8646288209607, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790552, Length=223, Percent_Identity=42.152466367713, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=38.2222222222222, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=34.375, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=42.4242424242424, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=35.0649350649351, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=36.0869565217391, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=33.7606837606838, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=38.8888888888889, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=30.5785123966942, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI48994928, Length=117, Percent_Identity=35.8974358974359, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=112, Percent_Identity=31.25, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25971; Mature: 25971

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVLIVEDEEALTTLLRYNLEAEGFLVDSAARGDDAELLLAERIPDLVLLDWMLPGLSGI
CEEEEECCHHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHCCHHEEHHHHHCCCCHH
ELCRRIRARRETERLPVIMLTARGEEGDRVRGLGTGADDYIVKPFSVPELLARIRALLRR
HHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCCCEECCCCHHHHHHHHHHHHHH
AKPAHVSDRLAAGDLELDRTGHRVRRGGEELHLGPTEFRLLEFLMLAPGRVFSREQLLDG
CCCCCCHHHHCCCCCEECCCCHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHCC
VWGHDVYIDERTVDVHVGRLRKALNGPRQADPIRTVRGSGYAFDETFSLEA
CCCCEEEEECCEEEHHHHHHHHHHCCCCCCCCHHHHCCCCCEECCEEECCC
>Mature Secondary Structure
MQVLIVEDEEALTTLLRYNLEAEGFLVDSAARGDDAELLLAERIPDLVLLDWMLPGLSGI
CEEEEECCHHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHCCHHEEHHHHHCCCCHH
ELCRRIRARRETERLPVIMLTARGEEGDRVRGLGTGADDYIVKPFSVPELLARIRALLRR
HHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCCCEECCCCHHHHHHHHHHHHHH
AKPAHVSDRLAAGDLELDRTGHRVRRGGEELHLGPTEFRLLEFLMLAPGRVFSREQLLDG
CCCCCCHHHHCCCCCEECCCCHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHCC
VWGHDVYIDERTVDVHVGRLRKALNGPRQADPIRTVRGSGYAFDETFSLEA
CCCCEEEEECCEEEHHHHHHHHHHCCCCCCCCHHHHCCCCCEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]