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The map label for this gene is dusA [H]

Identifier: 170748394

GI number: 170748394

Start: 2103887

End: 2104846

Strand: Reverse

Name: dusA [H]

Synonym: Mrad2831_1976

Alternate gene names: 170748394

Gene position: 2104846-2103887 (Counterclockwise)

Preceding gene: 170748398

Following gene: 170748390

Centisome position: 34.63

GC content: 75.73

Gene sequence:

>960_bases
ATGATGGACTGGACGGACCGGCACTGCCGGGCGTTCCACCGGTCGCTGTCGGCGCGCGCCCGCCTCTACACCGAGATGGT
GACCACCGGCGCGGTGCTGCACGGGCCGCGCGAGCGGCTGCTCGGCTTCGCGGCGGTGGAGCACCCGGTGGCGGTCCAGC
TCGGCGGCTCGGACCCGGGGGAGCTGGCGCAGGCCGCCCGCATCGCCGCGGATCTCGGCTACGACGAGGTCAATCTGAAT
GTCGGCTGCCCCTCGGACCGGGTGCAGGAGGGCCGCTTCGGCGCCTGCCTGATGCGCGAGCCGGCCCTGGTGGGTGACTG
CGTCGCCGCCATGAAGGCGGCGGTCCCCGTGCCGGTGACGGTGAAGTGCCGGATCGGCGTCGACGATCAGGACCCCGAGG
CGGCGCTCGACGCCCTGGCCGACGCCGTGGTGGCGGCGGGCGTCGACGGGCTGATCGTCCACGCCCGCAAGGCCTGGCTC
CAGGGCCTCTCGCCCAAGGAGAACCGGGACGTGCCCCCCCTCGATTACGGCCGCGCCGCGCGGCTGAAGCGGCGGCTTCC
CGGGCTGCCGATCGCGGTCAACGGCGGCCTGCGCGACCTGGATTCCGCGCGGGCGCGGCTCGCCGAGGTCGACGGCGTCA
TGGTCGGCCGCGCCGCCTACACCGAGCCGGCACTGCTGCTCGGCGTCGATCCCGACCTGTTCGGCGTGCCGGCCCCGGCC
CCCGACGTTTTCGCGGCGGTCGCGATGTTCGAGCCCTACATCGCCGCGTGCCTGGCCCGGGGCGAGCGCCTGCACGCGGT
CACCCGGCACATGCTGGGACTGTTCAACGGTCGGCCGGGCGCGCGGGCCTTCCGGCGCCACCTCTCGACCCACGGGATGC
GCCCGGAGGCCGACCTCGCGACCCTGCGGGAGGCGGTGGCGCTGGTCTCACGCGCCGCGCCCGAGGCGCGCGCCGCCTGA

Upstream 100 bases:

>100_bases
CGATGGGGCCCGGCGAGGTACAGCACGAGAGCGACAGCAAGTGATTGCCGCAACGGAAGAACCTGGAAATCTCAGCGGAT
GGCGCTTCTCCGTCGCCCCG

Downstream 100 bases:

>100_bases
GCGGCGGCCCGGTCAGGGCCGGCGCAGGATCAGGCGGTCGCCCCGGACCTCGTAGCCGGACAGGCGCGACAGGAAGGTCT
GGCCGAGGAGATTCGCCGAG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MMDWTDRHCRAFHRSLSARARLYTEMVTTGAVLHGPRERLLGFAAVEHPVAVQLGGSDPGELAQAARIAADLGYDEVNLN
VGCPSDRVQEGRFGACLMREPALVGDCVAAMKAAVPVPVTVKCRIGVDDQDPEAALDALADAVVAAGVDGLIVHARKAWL
QGLSPKENRDVPPLDYGRAARLKRRLPGLPIAVNGGLRDLDSARARLAEVDGVMVGRAAYTEPALLLGVDPDLFGVPAPA
PDVFAAVAMFEPYIAACLARGERLHAVTRHMLGLFNGRPGARAFRRHLSTHGMRPEADLATLREAVALVSRAAPEARAA

Sequences:

>Translated_319_residues
MMDWTDRHCRAFHRSLSARARLYTEMVTTGAVLHGPRERLLGFAAVEHPVAVQLGGSDPGELAQAARIAADLGYDEVNLN
VGCPSDRVQEGRFGACLMREPALVGDCVAAMKAAVPVPVTVKCRIGVDDQDPEAALDALADAVVAAGVDGLIVHARKAWL
QGLSPKENRDVPPLDYGRAARLKRRLPGLPIAVNGGLRDLDSARARLAEVDGVMVGRAAYTEPALLLGVDPDLFGVPAPA
PDVFAAVAMFEPYIAACLARGERLHAVTRHMLGLFNGRPGARAFRRHLSTHGMRPEADLATLREAVALVSRAAPEARAA
>Mature_319_residues
MMDWTDRHCRAFHRSLSARARLYTEMVTTGAVLHGPRERLLGFAAVEHPVAVQLGGSDPGELAQAARIAADLGYDEVNLN
VGCPSDRVQEGRFGACLMREPALVGDCVAAMKAAVPVPVTVKCRIGVDDQDPEAALDALADAVVAAGVDGLIVHARKAWL
QGLSPKENRDVPPLDYGRAARLKRRLPGLPIAVNGGLRDLDSARARLAEVDGVMVGRAAYTEPALLLGVDPDLFGVPAPA
PDVFAAVAMFEPYIAACLARGERLHAVTRHMLGLFNGRPGARAFRRHLSTHGMRPEADLATLREAVALVSRAAPEARAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI145693211, Length=311, Percent_Identity=54.6623794212219, Blast_Score=341, Evalue=3e-95,
Organism=Escherichia coli, GI1788462, Length=209, Percent_Identity=34.9282296650718, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789660, Length=229, Percent_Identity=28.82096069869, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=30.5164319248826, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=147, Percent_Identity=31.2925170068027, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24580595, Length=219, Percent_Identity=30.1369863013699, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920448, Length=219, Percent_Identity=30.1369863013699, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 33942; Mature: 33942

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDWTDRHCRAFHRSLSARARLYTEMVTTGAVLHGPRERLLGFAAVEHPVAVQLGGSDPG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCHH
ELAQAARIAADLGYDEVNLNVGCPSDRVQEGRFGACLMREPALVGDCVAAMKAAVPVPVT
HHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEE
VKCRIGVDDQDPEAALDALADAVVAAGVDGLIVHARKAWLQGLSPKENRDVPPLDYGRAA
EEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
RLKRRLPGLPIAVNGGLRDLDSARARLAEVDGVMVGRAAYTEPALLLGVDPDLFGVPAPA
HHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCEEECCHHCCCCEEEEECCCCCCCCCCCC
PDVFAAVAMFEPYIAACLARGERLHAVTRHMLGLFNGRPGARAFRRHLSTHGMRPEADLA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHH
TLREAVALVSRAAPEARAA
HHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MMDWTDRHCRAFHRSLSARARLYTEMVTTGAVLHGPRERLLGFAAVEHPVAVQLGGSDPG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCHH
ELAQAARIAADLGYDEVNLNVGCPSDRVQEGRFGACLMREPALVGDCVAAMKAAVPVPVT
HHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEE
VKCRIGVDDQDPEAALDALADAVVAAGVDGLIVHARKAWLQGLSPKENRDVPPLDYGRAA
EEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
RLKRRLPGLPIAVNGGLRDLDSARARLAEVDGVMVGRAAYTEPALLLGVDPDLFGVPAPA
HHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCEEECCHHCCCCEEEEECCCCCCCCCCCC
PDVFAAVAMFEPYIAACLARGERLHAVTRHMLGLFNGRPGARAFRRHLSTHGMRPEADLA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHH
TLREAVALVSRAAPEARAA
HHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]