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The map label for this gene is panB

Identifier: 170748380

GI number: 170748380

Start: 2092116

End: 2092934

Strand: Reverse

Name: panB

Synonym: Mrad2831_1962

Alternate gene names: 170748380

Gene position: 2092934-2092116 (Counterclockwise)

Preceding gene: 170748381

Following gene: 170748379

Centisome position: 34.44

GC content: 69.35

Gene sequence:

>819_bases
ATGTCGCAGGTCGTGCAGTCCCGACGGCTCCGGGCGATCGATATCGGCAAGCGCAAGGCCGAGGGGCGCAAGATCATCGC
GCTCACCGCCTACCACGCCCACACGGCCGGCATCGTCGATCCCTATTGCGACTTCATCCTCGTGGGCGATTCCCTCGGCA
TGGTCATGCACGGGATGGAATCGACGATCCCCGTGACCCTGGAGATGATGATCCTCCAGGCCCAGGCGGTGATCCGCGGC
ACCGAGAAGGCGCTGGTCGTGGTCGACATGCCGTTCGGCTCCTACGAGGCGAGCCGGGAGCAGGCCTTCCTGAACGCCTC
CCGGGTCCTGAAGGAGACCGGCGCCGGCGCCATCAAGATGGAGGGCGGCGCCCATTTCGCCGAGACGGTGGCGTTCCTCG
TCCAGCGCGGCGTTCCGGTGATGGGCCATATCGGCCTCACCCCGCAGGCGGTGAACACGATGGGCGGCTTCAAGGTGCAG
GGCCGGGGGCCGGACGACGAGCGCCGACTCCTGGAGGATGCCCGCGCCATCTCGGAGGCCGGCGCCTTCGCGATCGTGCT
CGAGGGCATCGTCGAGCCGGTGGCGCGGGCGATCGCGTCCTCGCCCCGGGTCACCGCCGCCACGATCGGCATCGGCGCCT
CGGCGGTCTGCGACGGGCAGATCCTGGTTCTGGAGGACATGCTCGGGCTGTCCGAGCGCACGCCGAAATTCGTGCGCCAG
TTCGGGAGCCTGCGGACCCACATCGAGGACGCCGTGAAGGCCTACGCCGATGAGGTCCGGGCCGGGCGGTTCCCGGCCGA
CGACCACACCTACGGGTAG

Upstream 100 bases:

>100_bases
AGAGAGGCCCGGAACGAGGCTCACGGAGCGGGCGCGCCCGCTCCCGGGCGGGAGAGGGGACCCGCGCCTCGTCCTGGGTG
GCCGGAATGAGGTCTGAACC

Downstream 100 bases:

>100_bases
GGCAGGGCGGCCCATGAAGCGCGTGATCGGGCCGTGGAGCGCTGCCGCCGCGTGGCCTGTCCGCGCGCTCGCGGTCTGCG
CCGCGGTCGCCGGACCGGGA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSQVVQSRRLRAIDIGKRKAEGRKIIALTAYHAHTAGIVDPYCDFILVGDSLGMVMHGMESTIPVTLEMMILQAQAVIRG
TEKALVVVDMPFGSYEASREQAFLNASRVLKETGAGAIKMEGGAHFAETVAFLVQRGVPVMGHIGLTPQAVNTMGGFKVQ
GRGPDDERRLLEDARAISEAGAFAIVLEGIVEPVARAIASSPRVTAATIGIGASAVCDGQILVLEDMLGLSERTPKFVRQ
FGSLRTHIEDAVKAYADEVRAGRFPADDHTYG

Sequences:

>Translated_272_residues
MSQVVQSRRLRAIDIGKRKAEGRKIIALTAYHAHTAGIVDPYCDFILVGDSLGMVMHGMESTIPVTLEMMILQAQAVIRG
TEKALVVVDMPFGSYEASREQAFLNASRVLKETGAGAIKMEGGAHFAETVAFLVQRGVPVMGHIGLTPQAVNTMGGFKVQ
GRGPDDERRLLEDARAISEAGAFAIVLEGIVEPVARAIASSPRVTAATIGIGASAVCDGQILVLEDMLGLSERTPKFVRQ
FGSLRTHIEDAVKAYADEVRAGRFPADDHTYG
>Mature_271_residues
SQVVQSRRLRAIDIGKRKAEGRKIIALTAYHAHTAGIVDPYCDFILVGDSLGMVMHGMESTIPVTLEMMILQAQAVIRGT
EKALVVVDMPFGSYEASREQAFLNASRVLKETGAGAIKMEGGAHFAETVAFLVQRGVPVMGHIGLTPQAVNTMGGFKVQG
RGPDDERRLLEDARAISEAGAFAIVLEGIVEPVARAIASSPRVTAATIGIGASAVCDGQILVLEDMLGLSERTPKFVRQF
GSLRTHIEDAVKAYADEVRAGRFPADDHTYG

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=257, Percent_Identity=43.1906614785992, Blast_Score=190, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319653, Length=268, Percent_Identity=35.4477611940299, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_METRJ (B1LTL1)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754640.1
- ProteinModelPortal:   B1LTL1
- SMR:   B1LTL1
- GeneID:   6137991
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_1962
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29119; Mature: 28988

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: ACT_SITE 188-188 BINDING 89-89 BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVVQSRRLRAIDIGKRKAEGRKIIALTAYHAHTAGIVDPYCDFILVGDSLGMVMHGME
CCHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHCCC
STIPVTLEMMILQAQAVIRGTEKALVVVDMPFGSYEASREQAFLNASRVLKETGAGAIKM
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
EGGAHFAETVAFLVQRGVPVMGHIGLTPQAVNTMGGFKVQGRGPDDERRLLEDARAISEA
CCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHC
GAFAIVLEGIVEPVARAIASSPRVTAATIGIGASAVCDGQILVLEDMLGLSERTPKFVRQ
CHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCEEEEEHHHHCCCCCCHHHHHH
FGSLRTHIEDAVKAYADEVRAGRFPADDHTYG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SQVVQSRRLRAIDIGKRKAEGRKIIALTAYHAHTAGIVDPYCDFILVGDSLGMVMHGME
CHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHCCC
STIPVTLEMMILQAQAVIRGTEKALVVVDMPFGSYEASREQAFLNASRVLKETGAGAIKM
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
EGGAHFAETVAFLVQRGVPVMGHIGLTPQAVNTMGGFKVQGRGPDDERRLLEDARAISEA
CCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHC
GAFAIVLEGIVEPVARAIASSPRVTAATIGIGASAVCDGQILVLEDMLGLSERTPKFVRQ
CHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCEEEEEHHHHCCCCCCHHHHHH
FGSLRTHIEDAVKAYADEVRAGRFPADDHTYG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA